scholarly journals Paraburkholderia madseniana sp. nov., a phenolic acid-degrading bacterium isolated from acidic forest soil

2020 ◽  
Vol 70 (3) ◽  
pp. 2137-2146 ◽  
Author(s):  
Roland C. Wilhelm ◽  
Sean J. L. Murphy ◽  
Nicole M. Feriancek ◽  
David C. Karasz ◽  
Christopher M. DeRito ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Forest Soil ◽  
Phthalic Acid ◽  
Type Species ◽  
Rrna Gene ◽  
Hydroxybenzoic Acid ◽  
Content Type ◽  
Link Type ◽  
Degrading Bacterium ◽  
Type Strains

RP11T was isolated from forest soil following enrichment with 4-hydroxybenzoic acid. Cells of RP11T are aerobic, non-sporulating, exhibit swimming motility, and are rods (0.8 µm by 1.4 µm) that often occur as diplobacillus or in short chains (3–4 cells). Optimal growth on minimal media containing 4-hydroxybenzoic acid (µ=0.216 hr−1) occurred at 30 °C, pH 6.5 or 7.0 and 0% salinity. Comparative chemotaxonomic, genomic and phylogenetic analyses revealed the isolate was distinct from its closest relative type strains identified as Paraburkholderia aspalathi LMG 27731T, Paraburkholderia fungorum LMG 16225T and Paraburkholderia caffeinilytica CF1T. Strain RP11T is genetically distinct from P. aspalathi , its closest relative, in terms of 16S rRNA gene sequence similarity (98.7%), genomic average nucleotide identity (94%) and in silico DNA–DNA hybridization (56.7 %±2.8). The composition of fatty acids and substrate utilization pattern differentiated strain RP11T from its closest relatives, including growth on phthalic acid. Strain RP11T encoded the greatest number of aromatic degradation genes of all eleven closely related type strains and uniquely encoded a phthalic acid dioxygenase and paralog of the 3-hydroxybenzoate 4-monooxygenase. The only ubiquinone detected in strain RP11T was Q-8, and the major cellular fatty acids were C16 : 0, 3OH-C16 : 0, C17 : 0 cyclo, C19 : 0 cyclo ω8c, and summed feature 8 (C18 : 1 ω7c/ω6c). On the basis of this polyphasic approach, it was determined that strain RP11T represents a novel species from the genus Paraburkholderia for which the name Paraburkholderia madseniana sp. nov. is proposed. The type strain is RP11T (=DSM 110123T=LMG 31517T).

scholarly journals Paraburkholderia solitsugae sp. nov. and Paraburkholderia elongata sp. nov., phenolic acid-degrading bacteria isolated from forest soil and emended description of Paraburkholderia madseniana

2020 ◽  
Vol 70 (9) ◽  
pp. 5093-5105 ◽  
Author(s):  
Roland C. Wilhelm ◽  
K. Taylor Cyle ◽  
Carmen Enid Martinez ◽  
David C. Karasz ◽  
Jeffrey D. Newman ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Forest Soil ◽  
Type Strain ◽  
Type Species ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Degrading Bacteria ◽  
Gene Similarity ◽  
Type Strains

Two bacterial strains, 1NT and 5NT, were isolated from hemlock forest soil using a soluble organic matter enrichment. Cells of 1NT (0.65×1.85 µm) and 5NT (0.6×1.85 µm) are Gram-stain-negative, aerobic, motile, non-sporulating and exist as single rods, diplobacilli or in chains of varying length. During growth in dilute media (≤0.1× tryptic soy broth; TSB), cells are primarily motile with flagella. At higher concentrations (≥0.3× TSB), cells of both strains increasingly form non-motile chains, and cells of 5NT elongate (0.57×~7 µm) and form especially long filaments. Optimum growth of 1NT and 5NT occurred at 25–30 °C, pH 6.5–7.0 and <0.5% salinity. Results of comparative chemotaxonomic, genomic and phylogenetic analyses revealed that 1NT and 5NT were distinct from one another and their closest related type strains: Paraburkholderia madseniana RP11T, Paraburkholderia aspalathi LMG 27731T and Paraburkholderia caffeinilytica CF1T. The genomes of 1NT and 5NT had an average nucleotide identity (91.6 and 91.3%) and in silico DNA–DNA hybridization values (45.8%±2.6 and 45.5%±2.5) and differed in functional gene content from their closest related type strains. The composition of fatty acids and patterns of substrate use, including the catabolism of phenolic acids, also differentiated strains 1NT and 5NT from each other and their closest relatives. The only ubiquinone present in strains 1NT and 5NT was Q-8. The major cellular fatty acids were C16 : 0, 3OH-C16 : 0, C17 : 0 cyclo, C19 : 0 cyclo ω8c and summed features 2 (3OH-C14 : 0 / C16 : 1 iso I), 3 (C16 : 1 ω6c/ω7c) and 8 (C18 : 1 ω7c/ω6c). A third bacterium, strain RL16-012-BIC-B, was isolated from soil associated with shallow roots and was determined to be a strain of P. madseniana (ANI, 98.8%; 16S rRNA gene similarity, 100%). Characterizations of strain RL16-012-BIC-B (DSM 110723=LMG 31706) led to proposed emendments to the species description of P. madseniana . Our polyphasic approach demonstrated that strains 1NT and 5NT represent novel species from the genus Paraburkholderia for which the names Paraburkholderia solitsugae sp. nov. (type strain 1NT=DSM 110721T=LMG 31704T) and Paraburkholderia elongata sp. nov. (type strain 5NT=DSM 110722T=LMG 31705T) are proposed.

scholarly journals Rhizobium flavum sp. nov., a triazophos-degrading bacterium isolated from soil under the long-term application of triazophos

2014 ◽  
Vol 64 (Pt_6) ◽  
pp. 2017-2022 ◽  
Author(s):  
Tao Gu ◽  
Li Na Sun ◽  
Jun Zhang ◽  
Xin Hua Sui ◽  
Shun Peng Li
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Species ◽  
Rrna Gene ◽  
Gene Sequences ◽  
Phenotypic Properties ◽  
Content Type ◽  
Link Type ◽  
Degrading Bacterium ◽  
Type Strains

A Gram-stain-negative, non-motile, pale yellow, rod-shaped bacterial strain, YW14T, was isolated from soil and its taxonomic position was investigated by a polyphasic study. Strain YW14T did not form nodules on three different legumes, and the nodD and nifH genes were not detected by PCR. Strain YW14T contained Q-10 as the predominant ubiquinone. The major cellular fatty acid was C18 : 1ω7c. Phylogenetic analyses based on 16S rRNA gene sequences and seven housekeeping gene sequences (recA, atpD, glnII, gyrB, rpoB, dnaK and thrC) showed that strain YW14T belonged to the genus Rhizobium . Strain YW14T showed 16S rRNA gene sequence similarity of 93.4–97.3 % to the type strains of recognized species of the genus Rhizobium . DNA–DNA relatedness between strain YW14T and the type strains of Rhizobium sullae IS123T and Rhizobium yanglingense CCBAU 71623T was 19.6–25.7 %, indicating that strain YW14T was distinct from them genetically. Strain YW14T could also be differentiated from these phylogenetically related species of the genus Rhizobium by various phenotypic properties. On the basis of phenotypic properties, phylogenetic distinctiveness and genetic data, strain YW14T is considered to represent a novel species of the genus Rhizobium , for which the name Rhizobium flavum sp. nov. is proposed. The type strain is YW14T ( = KACC 17222T = CCTCC AB2013042T).

scholarly journals Croceicoccus bisphenolivorans sp. nov., a bisphenol A-degrading bacterium isolated from seawater

2021 ◽  
Vol 71 (2) ◽  
Author(s):  
Jiangwei Li ◽  
Anyi Hu ◽  
Min Lv ◽  
Chang-Ping Yu
Keyword(s):  
Fatty Acids ◽  
Bisphenol A ◽  
Type Species ◽  
River Estuary ◽  
Rrna Gene ◽  
Phenotypic Data ◽  
Jiulong River Estuary ◽  
Content Type ◽  
Link Type ◽  
Degrading Bacterium

A bisphenol A-degrading bacterium, designated as strain H4T, was isolated from surface seawater, which was sampled from the Jiulong River estuary in southeast PR China. Strain H4T is Gram-stain-negative, aerobic, short rod-shaped, lacking bacteriochlorophyll a, motile with multifibrillar stalklike fascicle structures and capable of degrading bisphenol A. Growth of strain H4T was observed at 24–45 °C (optimum, 32 °C), at pH 5.5–9 (optimum, pH 7.0) and in 0–7 % NaCl (optimum, 2 %; w/v) . The 16S rRNA gene sequence of strain H4T showed highest similarity to Croceicoccus pelagius Ery9T (98.7 %), Croceicoccus sediminis (98.3 %), Croceicoccus naphthovorans PQ-2T (98.1 %) and Croceicoccus ponticola GM-16T (97.6 %), followed by Croceicoccus marinus E4A9T (96.7 %) and Croceicoccus mobilis Ery22T (96.0 %). Phylogenetic analysis revealed that strain H4T fell within a clade comprising the type strains of Croceicoccus species and formed a phyletic line with them that was distinct from other members of the family Erythrobacteraceae . The sole respiratory quinone was quinone 10 (Q-10). The predominant fatty acids (>5 % of the total fatty acids) of strain H4T were summed feature 8 (C18 : 1  ω6c and/or C18 : 1  ω7c), summed feature 3 (C16 : 1  ω6c and/or C16 : 1  ω7c), C17 : 1  ω6c and C14 : 02-OH. The genomic DNA G+C content was 62.8 mol%. In the polar lipid profile, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, two unidentified phospholipids, two sphingoglycolipids and three unknown lipids were the major compounds. Based on the genotypic and phenotypic data, strain H4T represents a novel species of the genus Croceicoccus , for which the name Croceicoccus bisphenolivorans sp. nov. is proposed. The type strain is H4T (=DSM 102182T=MCCC1 K02301T).

Novosphingobium lindaniclasticum sp. nov., a hexachlorocyclohexane (HCH)-degrading bacterium isolated from an HCH dumpsite

2013 ◽  
Vol 63 (Pt_6) ◽  
pp. 2160-2167 ◽  
Author(s):  
Anjali Saxena ◽  
Shailly Anand ◽  
Ankita Dua ◽  
Naseer Sangwan ◽  
Fazlurrahman Khan ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Phylogenetic Analysis ◽  
Type Strain ◽  
Type Species ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Degrading Bacterium

A yellow-pigmented, Gram-negative, aerobic, non-motile, non-spore-forming, rod-shaped-bacterium, LE124T, was isolated from a hexachlorocyclohexane (HCH) dumpsite located in Lucknow, India. The type strain LE124T grew well with hexachlorocyclohexane as a sole carbon source, degrading it within 24 h of incubation. Phylogenetic analysis of strain LE124T showed highest 16S rRNA gene sequence similarity to Novosphingobium barchaimii LL02T (98.5 %), Novosphingobium panipatense SM16T (98.1 %), Novosphingobium soli CC-TPE-1T (97.9 %), Novosphingobium naphthalenivorans TUT562T (97.6 %), Novosphingobium mathurense SM117T (97.5 %) and Novosphingobium resinovorum NCIMB 8767T (97.5 %) and lower sequence similarity (<97 %) to all other members of the genus Novosphingobium . The DNA–DNA relatedness between strain LE124T and N. barchaimii LL02T and other related type strains was found to vary from 15 % to 45 % confirming that it represents a novel species. The genomic DNA G+C content of strain LE124T was 60.7 mol%. The predominant fatty acids were summed feature 8 (C18 : 1ω7c, 49.1 %), summed feature 3 (C16 : 1ω7c/C16 : 1ω6c, 19.9 %), C16 : 0 (6.7 %), C17 : 1ω6c (4.9 %) and a few hydroxyl fatty acids, C14 : 0 2-OH (9.4 %) and C16 : 0 2-OH (2.1 %). Polar lipids consisted mainly of phosphatidyldimethylethanolamine, phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, sphingoglycolipid and some unidentified lipids. The major respiratory quinone was ubiquinone Q-10. Spermidine was the major polyamine observed. Phylogenetic analysis, DNA–DNA hybridization, chemotaxonomic and phenotypic analysis support the conclusion that strain LE124T represents a novel species within the genus Novosphingobium for which we propose the name Novosphingbium lindaniclasticum sp. nov. The type strain is LE124T ( = CCM 7976T = DSM 25409T).

Aminivibrio pyruvatiphilus gen. nov., sp. nov., an anaerobic, amino-acid-degrading bacterium from soil of a Japanese rice field

2013 ◽  
Vol 63 (Pt_10) ◽  
pp. 3679-3686 ◽  
Author(s):  
Takuya Honda ◽  
Takashi Fujita ◽  
Akio Tonouchi
Keyword(s):  
Type Species ◽  
Rice Field ◽  
Enrichment Culture ◽  
Rrna Gene ◽  
Good Growth ◽  
Poor Growth ◽  
Content Type ◽  
Link Type ◽  
Degrading Bacterium ◽  
Type Strains

A novel anaerobic bacterium that could ferment amino acids and organic acids was isolated from an anaerobic, propionate-oxidizing enrichment culture originating from soil of a rice field in Japan. Cells of the isolate, designated strain 4F6ET, were Gram-staining-negative, oxidase- and catalase-negative, non-spore-forming, vibrio-shaped, motile rods (0.8×2.0–2.5 µm) with two or three lateral flagella. Growth occurred at 20–42 °C (optimum at 37–40 °C), at pH 6.4–8.4 (optimum at pH 7.3) and at 0–1.5 % (w/v) NaCl (optimum at 0–0.5 %). Good growth occurred on glycine, serine, cysteine, pyruvate and citrate, whereas poor growth was observed on threonine, glutamine, l-malate, α-ketoglutarate, peptone and Casamino acids. In co-culture with the hydrogen-utilizing methanogen Methanobacterium formicicum JCM 10132T, strain 4F6ET oxidized alanine, valine, leucine, isoleucine, methionine, aspartate, glutamate, histidine, asparagine and fumarate. Yeast extract was required for growth. The G+C content of genomic DNA was 61.9 mol%. A phylogenetic analysis based on comparison of the 16S rRNA gene sequence showed that the type strains of Fretibacterium fastidiosum , Aminobacterium colombiense and Aminobacterium mobile , members of the family Synergistaceae , were the closest relatives of strain 4F6ET, with low sequence similarities (89.3, 89.5 and 86.2 %, respectively). Strain 4F6ET contained iso-C13 : 0 (24.43 %), iso-C15 : 0 (16.47 %) and C19 : 1ω11c/C19 : 1ω9c (16.32 %) as the major fatty acids, which differed from those of F. fastidiosum , Aminobacterium colombiense and Aminobacterium mobile . On the basis of phenotypic, chemotaxonomic and phylogenetic differences between strain 4F6ET and the type strains of F. fastidiosum and Aminobacterium species, we propose that strain 4F6ET represents a novel genus and species, Aminivibrio pyruvatiphilus gen. nov., sp. nov. The type strain of Aminivibrio pyruvatiphilus is strain 4F6ET ( = JCM 18417T = DSM 25964T).

scholarly journals Zoogloea dura sp. nov., a N2-fixing bacterium isolated from forest soil and emendation of the genus Zoogloea and the species Zoogloea oryzae and Zoogloea ramigera

2020 ◽  
Vol 70 (10) ◽  
pp. 5312-5318 ◽  
Author(s):  
Ram Hari Dahal ◽  
Dhiraj Kumar Chaudhary ◽  
Dong-Uk Kim ◽  
Jaisoo Kim
Keyword(s):  
Forest Soil ◽  
Type Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Major Respiratory Quinone ◽  
Type Strains ◽  
Zoogloea Ramigera

A motile, Gram-stain-negative, rod-shaped bacterium, designated G-4-1-14T, was obtained from forest soil sampled at Gwanggyo mountain, Gyeonggi-do, Republic of Korea. Cells were colourless, aerobic, grew optimally at 28–35 °C and hydrolysed DNA and casein. Phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain G-4-1-14T formed a lineage within the genus Zoogloea . The closest members were Zoogloea resiniphila ATCC 70068T (98.6 % sequence similarity), Zoogloea caeni EMB43T (98.2 %), Zoogloea oryzae A-7T (97.7 %), Zoogloea ramigera IAM 12136T (96.9 %) and Zoogloea oleivorans BucT (96.2 %). The major respiratory quinone was ubiquinone-8 and the principal polar lipids were phosphatidylethanolamine, phosphatidyl-N-methylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The predominant cellular fatty acids were summed feature 3 (iso-C15 :0 2-OH/C16  : 1 ω7c) and C16 : 0. The DNA G+C content was 65.9 mol%. The average nucleotide identity and digital DNA–DNA hybridization relatedness values between strain G-4-1-14T and other type strains were ≤81.6 and ≤24.9 %, respectively, which are below the species demarcation thresholds. Based on the results of phenotypic, phylogenetic and genomic analyses, strain G-4-1-14T represents a novel species in the genus Zoogloea , for which the name Zoogloea dura sp. nov. is proposed. The type strain is G-4-1-14T (=KACC 21618T=NBRC 114358T). In addition, we propose emendation of the genus Zoogloea and the species Zoogloea oryzae and Zoogloea ramigera .

Sphingomonas yantingensis sp. nov., a mineral-weathering bacterium isolated from purplish paddy soil

2014 ◽  
Vol 64 (Pt_3) ◽  
pp. 1030-1034 ◽  
Author(s):  
Jing Huang ◽  
Zhi Huang ◽  
Zhen-Dong Zhang ◽  
Lin-Yan He ◽  
Xia-Fang Sheng
Keyword(s):  
Fatty Acids ◽  
Type Species ◽  
Mineral Weathering ◽  
Western China ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Phenotypic Characteristics ◽  
Content Type ◽  
Link Type ◽  
Type Strains

A novel type of mineral-weathering bacterium was isolated from purplish soils collected from Yanting (Sichuan, south-western China). Cells of strain 1007T were Gram-stain-negative and rod-shaped, motile and yellow-pigmented. The isolate was strictly aerobic, catalase- and oxidase-positive, and grew optimally at 28-30 °C and pH 6.0-7.0. The genomic DNA G+C content of strain 1007T was 67±0.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1007T belonged to the genus Sphingomonas and was most closely related to Sphingomonas pruni IFO 15498T (97.3 %), Sphingomonas mali IFO 15500T (97.2 %), Sphingomonas japonica KC7T (97.2 %) and Sphingomonas koreensis JSS26T (97.0 %). This affiliation of strain 1007T to the genus Sphingomonas was confirmed by the presence of Q-10 as the major ubiquinone, sphingoglycolipid, C14 : 0 2-OH and by the absence of 3-hydroxy fatty acids. The major polyamine was homospermidine. The main cellular fatty acids included summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0. Based on the low level of DNA–DNA relatedness (ranging from 26.1 % to 58.7 %) to these type strains of species of the genus Sphingomonas and unique phenotypic characteristics, strain 1007T represents a novel species of the genus Sphingomonas , for which the name Sphingomonas yantingensis sp. nov. is proposed. The type strain is 1007T ( = DSM 27244T = JCM 19201T = CCTCC AB 2013146T).

scholarly journals Burkholderia jiangsuensis sp. nov., a methyl parathion degrading bacterium, isolated from methyl parathion contaminated soil

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 3247-3253 ◽  
Author(s):  
Xu-Yun Liu ◽  
Chun-Xiu Li ◽  
Xiao-Jing Luo ◽  
Qi-Liang Lai ◽  
Jian-He Xu
Keyword(s):  
Type Species ◽  
Methyl Parathion ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Ethanolamine Phosphatidyl ◽  
Degrading Bacterium ◽  
Genotypic Characteristics ◽  
Type Strains

A methyl parathion (MP) degrading bacterial strain, designated MP-1T, was isolated from a waste land where pesticides were formerly manufactured in Jiangsu province, China. Polyphasic taxonomic studies showed that MP-1T is a Gram-stain-negative, non-spore-forming, rod-shaped and motile bacterium. The bacterium could grow at salinities of 0–1 % (w/v) and temperatures of 15–40 °C. Strain MP-1T could reduce nitrate to nitrite, utilize d-glucose and l-arabinose, but not produce indole, or hydrolyse gelatin. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that MP-1T belongs to the genus Burkholderia , showing highest sequence similarity to Burkholderia grimmiae DSM 25160T (98.5 %), and similar strains including Burkholderia zhejiangensis OP-1T (98.2 %), Burkholderia choica LMG 22940T (97.5 %), Burkholderia glathei DSM 50014T (97.4 %), Burkholderia terrestris LMG 22937T (97.2 %) and Burkholderia telluris LMG 22936T (97.0 %). In addition, the gyrB and recA gene segments of strain MP-1T exhibited less than 89.0 % and 95.1 % similarities with the most highly-related type strains indicated above. The G+C content of strain MP-1T was 62.6 mol%. The major isoprenoid quinone was ubiquinone Q-8. The predominant polar lipids comprised phosphatidyl ethanolamine, phosphatidyl glycerol, aminolipid and phospholipid. The principal fatty acids in strain MP-1T were C18 : 1ω7c/C18 : 1ω6c (23.3 %), C16 : 0 (16.8 %), cyclo-C17 : 0 (15.0 %), C16 : 1ω7c/C16 : 1ω6 (8.5 %), cyclo-C19 : 0ω8c (8.1 %), C16 : 1 iso I/C14 : 0 3-OH (5.7 %), C16 : 0 3-OH (5.6 %) and C16 : 02-OH (5.1 %). The DNA–DNA relatedness values between strain MP-1T and the three type strains ( B. grimmiae DSM 25160T, B. zhejiangensis OP-1T and B. glathei DSM 50014T) ranged from 24.6 % to 37.4 %. In accordance with phenotypic and genotypic characteristics, strain MP-1T represents a novel species of the genus Burkholderia , for which the name Burkholderia jiangsuensis sp. nov. is proposed, the type strain is MP-1T (LMG 27927T = MCCC 1K00250T).

scholarly journals Chitinophaga tropicalis sp. nov., isolated from forest soil

2020 ◽  
Vol 70 (6) ◽  
pp. 3859-3864 ◽  
Author(s):  
Xian-Jiao Zhang ◽  
Guang-Da Feng ◽  
Qing Yao ◽  
Jun Zhang ◽  
Le Thi Bich Phuong ◽  
...  
Keyword(s):  
Forest Soil ◽  
Dna Hybridization ◽  
National Park ◽  
Type Species ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Cat Tien National Park ◽  
Type Strains

A novel bacterial strain, designated ysch24T, was isolated from a forest soil sample collected from the Cat Tien National Park, southern Vietnam. Cells were Gram-stain-negative, aerobic, gliding, filamentous or rod-shaped. The results of 16S rRNA gene analyses revealed that strain ysch24T belongs to the genus Chitinophaga , and was most closely related to Chitinophaga silvisoli GDMCC 1.1411T (97.4 %), followed by Chitinophaga oryziterrae JCM 16595T (97.3 %) and Chitinophaga sancti NBRC 15057T (96.9 %). The average nucleotide identity and digital DNA–DNA hybridization values between strain ysch24T and closely related type strains were 72.0–74.0 % and 19.1–19.4 %, respectively. Major fatty acids were iso-C15 : 0, C16 : 1  ω5c and iso-C17 : 0 3-OH and the predominant respiratory quinone was menaquinone 7. Polar lipids consisted of phosphatidylethanolamine, four unidentified aminophospholipids, two unidentified phospholipids and four unidentified lipids. The genomic DNA G+C content was 45.6 mol%. The study clearly showed that strain ysch24T should represent a novel species of the genus Chitinophaga , for which the name Chitinophaga tropicalis sp. nov. is proposed. The type strain is ysch24T (=GDMCC 1.1355T=KACC 21527T).

Maribrevibacterium harenarium gen. nov., sp. nov., represented by a marine strain of the family Oceanospirillaceae

2021 ◽  
Vol 71 (7) ◽  
Author(s):  
Kunlian Mo ◽  
Qingjuan Wu ◽  
Yonghua Hu ◽  
Huiqin Huang
Keyword(s):  
Fatty Acids ◽  
Type Species ◽  
Polar Lipids ◽  
Rrna Gene ◽  
The Novel ◽  
Content Type ◽  
Cellular Fatty Acids ◽  
Link Type ◽  
The Family ◽  
Type Strains

A Gram-stain-negative, non-motile, facultatively anaerobic, short rod-shaped bacterium, designated HB171799T, was isolated from seacoast sandy soil collected at Qishui Bay, Hainan, PR China. The chemotaxonomic analysis revealed that the respiratory quinones were Q-8 and Q-7, and the major cellular fatty acids were summed feature 8 (comprising C18 : 1  ω7c and/or C18 : 1  ω6c), C16 : 0 and C18 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid. The size of the draft genome was 3.68 Mb with a DNA G+C content of 48.0 mol%. Results of phylogenetic analyses based on 16S rRNA gene and genome sequences showed that the novel isolate belonged to the family Oceanospirillaceae and formed a distinct subcluster at the base of the radiation of the genus Marinomonas . The highest sequence similarity (96.0 %) of the novel isolate was found to the type strains of Marinomonas fungiae JCM 18476T and Marinomonas ostreistagni DSM23425T. The whole genome-based phylogeny and differences in cellular fatty acids and polar lipids readily distinguished strain HB171799T from all the closely related validly published type strains. Strain HB171799T is therefore suggested to represent a novel species of a new genus in the family Oceanospirillaceae , for which the name Maribrevibacterium harenarium gen. nov., sp. nov. is proposed. The type strain is HB171799T (=CGMCC 1.16727T=JCM 33332T).

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