scholarly journals Rhodosalinus halophilus sp. nov., isolated from a marine solar saltern, and emended description of the genus Rhodosalinus

2019 ◽  
Vol 69 (10) ◽  
pp. 3305-3309 ◽  
Author(s):  
Zhen-Wei Liu ◽  
Tao-Jie Wang ◽  
Hao Yang ◽  
De-Chen Lu ◽  
Zong-Jun Du
Keyword(s):  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Emended Description

scholarly journals Planococcus salinarum sp. nov., isolated from a marine solar saltern, and emended description of the genus Planococcus

2010 ◽  
Vol 60 (4) ◽  
pp. 754-758 ◽  
Author(s):  
Jung-Hoon Yoon ◽  
So-Jung Kang ◽  
Soo-Young Lee ◽  
Ki-Hoon Oh ◽  
Tae-Kwang Oh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Rrna Gene Sequence ◽  
Marine Solar Saltern ◽  
Emended Description ◽  
Type Strains

A Gram-positive, non-motile and coccoid-, short rod- or rod-shaped bacterial strain, ISL-16T, was isolated from a marine solar saltern in Korea and its taxonomic position was investigated using a polyphasic taxonomic approach. Strain ISL-16T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain ISL-16T joined the cluster comprising species of the genus Planococcus. Its 16S rRNA gene sequence contained the same signature nucleotides as those defined for the genus Planococcus. Strain ISL-16T exhibited 16S rRNA gene sequence similarity values of 96.9–98.2 % to the type strains of species of the genus Planococcus. Strain ISL-16T contained MK-8 and MK-7 as the predominant menaquinones and anteiso-C15 : 0, C16 : 1 ω7c alcohol and anteiso-C17 : 0 as the major fatty acids. The DNA G+C content was 48.3 mol%. DNA–DNA relatedness values between strain ISL-16T and the type strains of species of the genus Planococcus were 15–28 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, enabled strain ISL-16T to be differentiated from recognized species of the genus Planococcus. On the basis of the data presented, strain ISL-16T is considered to represent a novel species of the genus Planococcus, for which the name Planococcus salinarum sp. nov. is proposed. The type strain is ISL-16T (=KCTC 13584T=CCUG 57753T). An emended description of the genus Planococcus is also given.

scholarly journals Halogeometricum rufum sp. nov., a halophilic archaeon from a marine solar saltern, and emended description of the genus Halogeometricum

2010 ◽  
Vol 60 (11) ◽  
pp. 2613-2617 ◽  
Author(s):  
Heng-Lin Cui ◽  
Xin Yang ◽  
Xia Gao ◽  
Xin-Yi Li ◽  
Xue-Wei Xu ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Halophilic Archaea ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Gene Sequence Analysis ◽  
Nacl Concentration ◽  
Optimum Ph ◽  
Emended Description ◽  
Halogeometricum Borinquense

Two halophilic archaea, strains RO1-4T and RO1-64, were isolated from a marine solar saltern in Jiangsu, China. Cells of the two strains were pleomorphic, motile, and stained Gram-negative. Colonies were red-pigmented. Strains RO1-4T and RO1-64 were able to grow at 25–55 °C (optimum 40–42 °C), at 2.1–5.1 M NaCl (optimum 3.9 M NaCl), at 0.05–0.7 M MgCl2 (optimum 0.3 M MgCl2) and at pH 6.0–8.5 (optimum pH 7.0). Cells lyse in distilled water and the minimal NaCl concentration to prevent cell lysis is 12 % (w/v). On the basis of 16S rRNA gene sequence analysis, strains RO1-4T and RO1-64 were closely related to Halogeometricum borinquense PR3T (98.0 and 98.2 % similarity, respectively) and Halosarcina pallida BZ256T (97.8 and 97.9 %). The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two glycolipids (S-DGD-1 and DGD-1). The DNA G+C contents of strains RO1-4T and RO1-64 are 64.9 and 62.4 mol%, respectively. The DNA–DNA hybridization value between strains RO1-4T and RO1-64 was 83.0 % and both strains showed low DNA–DNA relatedness with Halogeometricum borinquense PR3T (42.5 and 50.1 % relatedness, respectively) and Halosarcina pallida BZ256T (37.6 and 42.1 % relatedness). It was concluded that strains RO1-4T and RO1-64 represent a novel species of the genus Halogeometricum, for which the name Halogeometricum rufum sp. nov. is proposed. The type strain is RO1-4T (=CGMCC 1.7736T =JCM 15770T).

scholarly journals Halochromatium roseum sp. nov., a non-motile phototrophic gammaproteobacterium with gas vesicles, and emended description of the genus Halochromatium

2007 ◽  
Vol 57 (9) ◽  
pp. 2110-2113 ◽  
Author(s):  
P. Anil Kumar ◽  
T. N. R. Srinivas ◽  
Ch. Sasikala ◽  
Ch. V. Ramana
Keyword(s):  
Novel Species ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Gas Vesicles ◽  
16S Rrna Gene Sequences ◽  
Marine Solar Saltern ◽  
Purple Sulfur Bacterium ◽  
Absolute Requirement ◽  
Intracytoplasmic Membranes ◽  
Emended Description

A rod-shaped, marine, phototrophic, purple sulfur bacterium containing gas vesicles was isolated from a marine solar saltern at Kakinada, India. Cells of strain JA134T are Gram-negative, non-motile rods, with vesicular intracytoplasmic membranes, and the strain has an absolute requirement for NaCl for growth. Bacteriochlorophyll a and the carotenoid okenone are present as photosynthetic pigments. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain JA134T clusters with members of the genus Halochromatium, but is distinctly separated from the species reported so far. The morphological and physiological differences of strain JA134T from other Halochromatium species support the description of strain JA134T (=ATCC BAA-1363T =DSM 18859T =JCM 14151T) as the type strain of a novel species, Halochromatium roseum sp. nov.

Natronoarchaeum rubrum sp. nov., isolated from a marine solar saltern, and emended description of the genus Natronoarchaeum

2014 ◽  
Vol 64 (Pt_3) ◽  
pp. 952-956 ◽  
Author(s):  
Xing-Xing Qiu ◽  
Mei-Lin Zhao ◽  
Heng-Lin Cui
Keyword(s):  
16S Rrna ◽  
Type Species ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Content Type ◽  
Link Type ◽  
Emended Description

A halophilic archaeal strain, GX48T, was isolated from the Gangxi marine solar saltern near Weihai city in Shandong Province, China. Cells of the strain were rod-shaped, stained Gram-negative and formed red-pigmented colonies. Strain GX48T was able to grow at 25–50 °C (optimum 37 °C), in the presence of 1.4–4.8 M NaCl (optimum 2.6 M NaCl), with 0–1.0 M MgCl2 (optimum 0.05 M MgCl2) and at pH 5.5–9.5 (optimum pH 7.0). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two major glycolipids chromatographically identical to those of Natronoarchaeum mannanilyticum YSM-123T and Natronoarchaeum philippinense 294-194-5T. 16S rRNA gene analysis revealed that strain GX48T had two dissimilar 16S rRNA genes and both of them were phylogenetically related to those of the two current members of the genus Natronoarchaeum (96.2–98.3 % similarities). The rpoB′ gene sequence similarities between strain GX48T and Natronoarchaeum mannanilyticum YSM-123T and Natronoarchaeum philippinense 294-194-5T were 96.0 % and 94.7 %, respectively. The DNA G+C content of strain GX48T was 66.2 mol%. Strain GX48T showed low DNA–DNA relatedness with the two members of the genus Natronoarchaeum . It was concluded that strain GX48T ( = CGMCC 1.10388T = JCM 17119T) represents a novel species of the genus Natronoarchaeum , for which the name Natronoarchaeum rubrum sp. nov. is proposed. An emended description of the genus Natronoarchaeum is also presented.

scholarly journals Halomicroarcula limicola sp. nov., isolated from a marine solar saltern, and emended description of the genus Halomicroarcula

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1747-1751 ◽  
Author(s):  
Wen-Jiao Zhang ◽  
Heng-Lin Cui
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Content Type ◽  
Link Type ◽  
Nacl Concentration ◽  
Emended Description

Halophilic archaeal strain YGHS32T was isolated from the Yinggehai marine solar saltern near Shanya city of Hainan Province, China. Cells of the strain were pleomorphic and lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. Strain YGHS32T was able to grow at 20–50 °C (optimum 37 °C), in the presence of 0.9–4.8 M NaCl (optimum 2.1 M NaCl), with 0.005–1.0 M MgCl2 (optimum 0.3 M MgCl2) and at pH 6.0–8.5 (optimum pH 7.5). The minimal NaCl concentration to prevent cell lysis was 5 % (w/v). The major polar lipids of the strain were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and four major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether, mannosyl glucosyl diether, glucosyl mannosyl glucosyl diether and a diglycosyl diether. Strain YGHS32T had two dissimilar 16S rRNA genes and both of them were phylogenetically related to those of Halomicroarcula pellucida JCM 17820T (92.9–96.3 % sequence similarity). The rpoB′ gene sequence similarity between strain YGHS32T and Halomicroarcula pellucida JCM 17820T was 91.3 %. The DNA G+C content of strain YGHS32T was 64.0 mol%. The DNA–DNA hybridization value between strain YGHS32T and Halomicroarcula pellucida JCM 17820T was 45 %. It was concluded that strain YGHS32T ( = CGMCC 1.12129T = JCM 18640T) represents a novel species of the genus Halomicroarcula , for which the name Halomicroarcula limicola sp. nov. is proposed. An emended description of the genus Halomicroarcula is also presented.

scholarly journals Haladaptatus litoreus sp. nov., an extremely halophilic archaeon from a marine solar saltern, and emended description of the genus Haladaptatus

2010 ◽  
Vol 60 (5) ◽  
pp. 1085-1089 ◽  
Author(s):  
Heng-Lin Cui ◽  
Fang-Fang Sun ◽  
Xia Gao ◽  
Ying Dong ◽  
Xue-Wei Xu ◽  
...  
Keyword(s):  
Halophilic Archaea ◽  
Single Species ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Gene Sequence Analysis ◽  
Emended Description ◽  
Extremely Halophilic Archaeon ◽  
Low Levels ◽  
Ph Range

Two extremely halophilic archaea, strains RO1-28T and RO1-22, were isolated from a marine solar saltern in Jiangsu, China. Both strains required at least 0.05 M Mg2+ and 1.7 M NaCl for growth. They were able to grow over a pH range of 6.0–8.5 and a temperature range of 25–55 °C, with optimal pH of 7.0 and optimal temperature of 37–40 °C. Based on 16S rRNA gene sequence analysis, strains RO1-28T and RO1-22 were closely related to Haladaptatus paucihalophilus, the single species of the genus Haladaptatus, with similarities of 94.0–95.2 %. The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and three glycolipids chromatographically identical to the glycolipids of Haladaptatus paucihalophilus JCM 13897T. Both strains RO1-28T and RO1-22 had a DNA G+C content of 54.0 mol% (HPLC). The DNA–DNA hybridization value between the two strains was more than 70 % (92 %) and both strains showed low levels of DNA–DNA relatedness (32 % and 33 %) with Haladaptatus paucihalophilus JCM 13897T. It was concluded that strains RO1-28T and RO1-22 represent a novel species of the genus Haladaptatus, for which the name Haladaptatus litoreus sp. nov. is proposed. The type strain is RO1-28T (=CGMCC 1.7737T =JCM 15771T).

scholarly journals Haloplanus vescus sp. nov., an extremely halophilic archaeon from a marine solar saltern, and emended description of the genus Haloplanus

2010 ◽  
Vol 60 (8) ◽  
pp. 1824-1827 ◽  
Author(s):  
Heng-Lin Cui ◽  
Xia Gao ◽  
Xin-Yi Li ◽  
Xue-Wei Xu ◽  
Yu-Guang Zhou ◽  
...  
Keyword(s):  
Surface Strain ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Halophilic Archaeon ◽  
Marine Solar Saltern ◽  
Gene Sequence Analysis ◽  
Nacl Concentration ◽  
Optimum Ph ◽  
Emended Description ◽  
Extremely Halophilic Archaeon

An extremely halophilic archaeon, strain RO5-8T, was isolated from a disused marine solar saltern in China. The cells were pleomorphic and flat. In static liquid medium, cells floated to the surface. Strain RO5-8T stained Gram-negative and colonies were pink-pigmented. It was able to grow at 30–50 °C (optimum 40 °C), at 2.6–4.3 M NaCl (optimum 3.1 M NaCl), at 0.03–0.5 M MgCl2 (optimum 0.03 M MgCl2) and at pH 5.5–7.5 (optimum pH 6.0–6.5). Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 12 % (w/v). The major polar lipids of strain RO5-8T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and one major glycolipid chromatographically identical to the sulfated mannosyl glucosyl diether S-DGD-1. On the basis of 16S rRNA gene sequence analysis, strain RO5-8T was closely related to three strains of Haloplanus natans with similarities of 97.3–97.6 %. The DNA G+C content of strain RO5-8T was 62.1 mol%. The DNA–DNA hybridization value between strain RO5-8T and Haloplanus natans JCM 14081T was 51.6 %. It was concluded that strain RO5-8T represents a novel species of the genus Haloplanus, for which the name Haloplanus vescus sp. nov. is proposed. The type strain is RO5-8T (=CGMCC 1.8712T =JCM 16055T).

scholarly journals Halogranum gelatinilyticum sp. nov. and Halogranum amylolyticum sp. nov., isolated from a marine solar saltern, and emended description of the genus Halogranum

2011 ◽  
Vol 61 (4) ◽  
pp. 911-915 ◽  
Author(s):  
Heng-Lin Cui ◽  
Xin Yang ◽  
Xia Gao ◽  
Xue-Wei Xu
Keyword(s):  
Type Strain ◽  
Gene Sequence ◽  
Novel Species ◽  
Rrna Gene ◽  
Solar Saltern ◽  
Marine Solar Saltern ◽  
Dna Relatedness ◽  
Gene Sequence Analysis ◽  
Optimum Ph ◽  
Emended Description

Two extremely halophilic archaeal strains, designated TNN44T and TNN58T, were isolated from Tainan marine solar saltern near Lianyungang city, Jiangsu province, China. Cells of the two strains were pleomorphic and Gram-stain-negative; colonies were red-pigmented. Strains TNN44T and TNN58T were able to grow at 20–50 °C (optimum 37 °C for both), in the presence of 1.4–5.1 M NaCl (optimum 3.4–3.9 M NaCl) and at pH 5.5–9.0 (optimum pH 6.5–7.0); neither strain required Mg2+ for growth. Cells lysed in distilled water. On the basis of 16S rRNA gene sequence analysis, strains TNN44T and TNN58T were related closely to Halogranum rubrum RO2-11T (96.2 and 97.2 % similarity, respectively). The polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate (trace), and one major glycolipid and one minor glycolipid chromatographically identical to sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, respectively; other trace unidentified lipids were also detected. The DNA G+C content of strains TNN44T and TNN58T was 64.0 and 62.0 mol%, respectively. The level of DNA–DNA relatedness between strains TNN44T and TNN58T was 37.2 %, and these two strains showed a low level of DNA–DNA relatedness with Halogranum rubrum RO2-11T (40.6 and 44.4 %, respectively). Two novel species of the genus Halogranum are proposed to accommodate these two strains, Halogranum gelatinilyticum sp. nov. (type strain TNN44T  = CGMCC 1.10119T  = JCM 16426T) and Halogranum amylolyticum sp. nov. (type strain TNN58T  = CGMCC 1.10121T  = JCM 16428T).

scholarly journals Salegentibacter salinarum sp. nov., isolated from a marine solar saltern

2008 ◽  
Vol 58 (2) ◽  
pp. 365-369 ◽  
Author(s):  
J.-H. Yoon ◽  
M.-H. Lee ◽  
S.-J. Kang ◽  
T.-K. Oh
Keyword(s):  
Solar Saltern ◽  
Marine Solar Saltern
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