scholarly journals Rubrobacter spartanus sp. nov., a moderately thermophilic oligotrophic bacterium isolated from volcanic soil

2017 ◽  
Vol 67 (9) ◽  
pp. 3597-3602 ◽  
Author(s):  
Jeffrey S. Norman ◽  
Gary M. King ◽  
Maren L. Friesen
Keyword(s):  
Volcanic Soil ◽  
Moderately Thermophilic ◽  
Oligotrophic Bacterium

PEMANFAATAN TANAH VULKANIK DALAM SISTEM MULTIPLE SOIL LAYERING (MSL) TERHADAP PEMURNIAN AIR IRIGASI TERPOLUSI

Jurnal BiBieT ◽  
2017 ◽  
Vol 2 (2) ◽  
pp. 49
Author(s):  
Welly Herman ◽  
Darmawan Darmawan ◽  
Gusnidar Gusnidar
Keyword(s):  
Irrigation Water ◽  
Loading Rate ◽  
Quality Standard ◽  
High Ability ◽  
Volcanic Soil ◽  
Soil Mixture ◽  
Hydraulic Loading Rate ◽  
Hydraulic Loading ◽  
Soil Layering ◽  
Mesh Sieve

<p><em>The research aimed to make Volcanic soil of Multiple Soil Layering (MSL) with different Hydraulic Loading Rate (HLR) on the purification of polluted irrigation water and to determine the appropriate HLR against purification of polluted irrigation water. The research used an MSL system of the same Soil Mixture Block (SMB) size from a study done by </em><em>(Chen et al., 2007)</em><em> arranged in an Acrylic box measuring 50 cm x 10 cm x 60 cm (PxLxT). Making SMB is done by mixing volcanic soil, sawdust, iron, charcoal that has been mashed by 50 mesh sieve, with a combination of 7: 1: 1: 1. The MSL system is supplied with irrigation water taken from the Gunuang Nago irrigation and Pasar Baru area, Cupak Tangah village, Pauh IX sub-district, Padang continuously with different HLR of 250 L/m<sup>2</sup>/day,     500 L/m<sup>2</sup>/ day and 1000 L/m<sup>2</sup>/day. From the result of this research, it is found that MSL system can decrease pollutant content in polluted irrigation water until the concentration below the water quality standard based on PP. 82 of 2001 and MSL system with HLR 250 L/m<sup>2</sup>/day have high ability in purifying BOD and COD and HLR 1000 L/m<sup>2</sup>/day has a high ability in purifying NH<sub>4</sub><sup>+</sup>, NO<sub>2</sub><sup>-</sup> and NO<sub>3</sub><sup>-</sup> on polluted irrigation water.</em></p><p class="jbd-alamat"> </p><p class="jbd-alamat">Tujuan penelitian ini adalah untuk menentukan pengaruh tanah vulkanik dalam sistem <em>Multiple Soil Layering</em> (MSL) dengan <em>Hydraulic Loading Rate</em> (HLR) yang berbeda terhadap pemurnian air irigasi terpolusi. Penelitian  menggunakan sistem MSL dengan ukuran <em>Soil Mixture Block</em> (SMB) yang sama dari penelitian yang telah dilakukan (Chen, Sato, Wakatsuki, &amp; Masunaga, 2007)yang disusun di dalam kotak Acrylic berukuran 50 cm x 10 cm x 60 cm (PxLxT).  <em>Soil Mixture Block</em> terdiri dari tanah vulkanik, serbuk gergaji, besi, arang yang telah dihaluskan oleh ayakan 50 mesh, dengan perbandingan kombinasi 7:1:1:1.  Sistem MSL dialirkan air irigasi yang diambil dari irigasi Gunuang Nago dan Kawasan Pasar Baru, Kelurahan Cupak Tangah, Kecamatan Pauh IX, Padang secara terus menerus dengan HLR yang berbeda yaitu 250 L/m<sup>2</sup>/hari,  500 L/m<sup>2</sup>/hari dan 1000 L/m<sup>2</sup>/hari. Dari hasil penelitian diperoleh bahwa sistem MSL mampu menurunkan kandungan zat pencemar pada air irigasi terpolusi  mencapai kosentrasi di bawah baku mutu air berdasarkan PP No. 82 tahun 2001 dengan HLR 250 L/m<sup>2</sup>/hari mempunyai kemampuan yang tinggi dalam memurnikan kadar pencemar BOD dan COD sedangkan HLR 1000 L/m2/hari mempunyai kemampuan yang tinggi dalam memurnikan kadar pencemar NH<sub>4</sub><sup>+</sup>, NO<sub>2</sub><sup>-</sup> dan NO<sub>3</sub><sup>-</sup> pada air irigasi terpolusi.</p>

Ferrous iron oxidation in moderately thermophilic acidophile Sulfobacillus sibiricus N1T

2010 ◽  
Vol 56 (10) ◽  
pp. 803-808 ◽  
Author(s):  
Tatiana Y. Dinarieva ◽  
Anna E. Zhuravleva ◽  
Oksana A. Pavlenko ◽  
Iraida A. Tsaplina ◽  
Alexander I. Netrusov
Keyword(s):  
Ferrous Iron ◽  
High Performance ◽  
Iron Oxidation ◽  
Early Exponential Phase ◽  
Moderately Thermophilic ◽  
Terminal Oxidases ◽  
Cytochrome Bo ◽  
Ferrous Iron Oxidation ◽  
Transport Chain ◽  
Membrane Bound

The iron-oxidizing system of a moderately thermophilic, extremely acidophilic, gram-positive mixotroph, Sulfobacillus sibiricus N1T, was studied by spectroscopic, high-performance liquid chromatography and inhibitory analyses. Hemes B, A, and O were detected in membranes of S. sibiricus N1T. It is proposed that the electron transport chain from Fe2+ to O2 is terminated by 2 physiological oxidases: aa3-type cytochrome, which dominates in the early-exponential phase of growth, and bo3-type cytochrome, whose role in iron oxidation becomes more prominent upon growth of the culture. Both oxidases were sensitive to cyanide and azide. Cytochrome aa3 was more sensitive to cyanide and azide, with Ki values of 4.1 and 2.5 µmol·L–1, respectively, compared with Ki values for cytochrome bo3, which were 9.5 µmol·L–1 for cyanide and 7.0 µmol·L–1 for azide. This is the first evidence for the participation of a bo3-type oxidase in ferrous iron oxidation. The respiratory chain of the mixotroph contains, in addition to the 2 terminal oxidases, a membrane-bound cytochrome b573.

scholarly journals Decomposition of Rice Chaff Using a Cocultivation System of Thermobifida fusca and Ureibacillus thermosphaericus

Proceedings ◽  
2021 ◽  
Vol 66 (1) ◽  
pp. 31
Author(s):  
Sachiko Nakamura ◽  
Norio Kurosawa
Keyword(s):  
Lignin Peroxidase ◽  
Culture Medium ◽  
Reducing Sugars ◽  
Thermophilic Bacteria ◽  
Thermobifida Fusca ◽  
Bacterial Strains ◽  
Basal Salt Medium ◽  
Moderately Thermophilic ◽  
Fuels And Chemicals ◽  
Ureibacillus Thermosphaericus

Lignocellulosic biomass comprises cellulose, hemicellulose, and lignin and is a potential source of fuels and chemicals. Although this complex biomass is persistent, it can be cooperatively decomposed by a microbial consortium in nature. In this study, a coculture of the moderately thermophilic bacteria Thermobifida fusca and Ureibacillus thermosphaericus was used for biodegradation of rice chaff. The bacterial strains were incubated in modified Brock’s basal salt medium (pH 8.0) supplemented with yeast extract and rice chaff at 50 °C for 7 days. The concentration of reducing sugars and the enzymatic activities of laccase, lignin peroxidase, cellulase, and xylanase in the supernatant of the culture medium were measured every day. The concentrations of reducing sugars in solo cultures of T. fusca and U. thermosphaericus and a mixed culture of the two strains after 7 days of incubation were 0.047, 0.040, and 0.195 mg/mL, respectively, indicating that the decomposition of rice chaff was enhanced in the coculture. Based on the results, it is thought that the lignin surrounding the cellulose was decomposed by laccase and lignin peroxidase secreted from U. thermosphaericus, resulting in cellulose and hemicellulose in the rice chaff being easily decomposed by enzymes from T. fusca.

scholarly journals Membrane Lipid Composition of the Moderately Thermophilic Ammonia-Oxidizing Archaeon “Candidatus Nitrosotenuis uzonensis” at Different Growth Temperatures

2019 ◽  
Vol 85 (20) ◽  
Author(s):  
Nicole J. Bale ◽  
Marton Palatinszky ◽  
W. Irene C. Rijpstra ◽  
Craig W. Herbold ◽  
Michael Wagner ◽  
...  
Keyword(s):  
Lipid Composition ◽  
Strong Influence ◽  
Thermal Spring ◽  
Membrane Lipid ◽  
Effect Of Temperature ◽  
Environmental Niche ◽  
Membrane Lipid Composition ◽  
Moderately Thermophilic ◽  
Wide Range ◽  
Membrane Spanning

ABSTRACT “Candidatus Nitrosotenuis uzonensis” is the only cultured moderately thermophilic member of the thaumarchaeotal order Nitrosopumilales (NP) that contains many mesophilic marine strains. We examined its membrane lipid composition at different growth temperatures (37°C, 46°C, and 50°C). Its lipids were all membrane-spanning glycerol dialkyl glycerol tetraethers (GDGTs), with 0 to 4 cyclopentane moieties. Crenarchaeol (cren), the characteristic thaumarchaeotal GDGT, and its isomer (crenʹ) were present in high abundance (30 to 70%). The GDGT polar headgroups were mono-, di-, and trihexoses and hexose/phosphohexose. The ratio of glycolipid to phospholipid GDGTs was highest in the cultures grown at 50°C. With increasing growth temperatures, the relative contributions of cren and crenʹ increased, while those of GDGT-0 to GDGT-4 (including isomers) decreased. TEX86 (tetraether index of tetraethers consisting of 86 carbons)-derived temperatures were much lower than the actual growth temperatures, further demonstrating that TEX86 does not accurately reflect the membrane lipid adaptation of thermophilic Thaumarchaeota. As the temperature increased, specific GDGTs changed relative to their isomers, possibly representing temperature adaption-induced changes in cyclopentane ring stereochemistry. Comparison of a wide range of thaumarchaeotal core lipid compositions revealed that the “Ca. Nitrosotenuis uzonensis” cultures clustered separately from other members of the NP order and the Nitrososphaerales (NS) order. While phylogeny generally seems to have a strong influence on GDGT distribution, our analysis of “Ca. Nitrosotenuis uzonensis” demonstrates that its terrestrial, higher-temperature niche has led to a lipid composition that clearly differentiates it from other NP members and that this difference is mostly driven by its high crenʹ content. IMPORTANCE For Thaumarchaeota, the ratio of their glycerol dialkyl glycerol tetraether (GDGT) lipids depends on growth temperature, a premise that forms the basis of the widely applied TEX86 paleotemperature proxy. A thorough understanding of which GDGTs are produced by which Thaumarchaeota and what the effect of temperature is on their GDGT composition is essential for constraining the TEX86 proxy. “Ca. Nitrosotenuis uzonensis” is a moderately thermophilic thaumarchaeote enriched from a thermal spring, setting it apart in its environmental niche from the other marine mesophilic members of its order. Indeed, we found that the GDGT composition of “Ca. Nitrosotenuis uzonensis” cultures was distinct from those of other members of its order and was more similar to those of other thermophilic, terrestrial Thaumarchaeota. This suggests that while phylogeny has a strong influence on GDGT distribution, the environmental niche that a thaumarchaeote inhabits also shapes its GDGT composition.

scholarly journals Identification of Poly(cis-1,4-Isoprene) Degradation Intermediates during Growth of Moderately Thermophilic Actinomycetes on Rubber and Cloning of a Functional lcp Homologue from Nocardia farcinica Strain E1

2006 ◽  
Vol 72 (5) ◽  
pp. 3375-3382 ◽  
Author(s):  
Ebaid M. A. Ibrahim ◽  
Matthias Arensk�tter ◽  
Heinrich Luftmann ◽  
Alexander Steinb�chel
Keyword(s):  
Thermophilic Bacteria ◽  
Natural Rubber Latex ◽  
Gel Permeation Chromatography ◽  
Mycolic Acid ◽  
Rrna Gene ◽  
Ionization Mass ◽  
Thermophilic Actinomycetes ◽  
Moderately Thermophilic ◽  
Nocardia Farcinica ◽  
Different Strains

ABSTRACT The enrichment and isolation of thermophilic bacteria capable of rubber [poly(cis-1,4-isoprene)] degradation revealed eight different strains exhibiting both currently known strategies used by rubber-degrading mesophilic bacteria. Taxonomic characterization of these isolates by 16S rRNA gene sequence analysis demonstrated closest relationships to Actinomadura nitritigenes, Nocardia farcinica, and Thermomonospora curvata. While strains related to N. farcinica exhibited adhesive growth as described for mycolic acid-containing actinomycetes belonging to the genus Gordonia, strains related to A. nitritigenes and T. curvata formed translucent halos on natural rubber latex agar as described for several mycelium-forming actinomycetes. For all strains, optimum growth rates were observed at 50�C. The capability of rubber degradation was confirmed by mineralization experiments and by gel permeation chromatography (GPC). Intermediates resulting from early degradation steps were purified by preparative GPC, and their analysis by infrared spectroscopy revealed the occurrence of carbonyl carbon atoms. Staining with Schiff's reagent also revealed the presence of aldehyde groups in the intermediates. Bifunctional isoprenoid species terminated with a keto and aldehyde function were found by matrix-assisted laser desorption ionization-time-of-flight and electrospray ionization mass spectrometry analyses. Evidence was obtained that biodegradation of poly(cis-1,4-isoprene) is initiated by endocleavage, rather than by exocleavage. A gene (lcp) coding for a protein with high homology to Lcp (latex-clearing protein) from Streptomyces sp. strain K30 was identified in Nocardia farcinica E1. Streptomyces lividans TK23 expressing this Lcp homologue was able to cleave synthetic poly(cis-1,4-isoprene), confirming its involvement in initial polymer cleavage.

scholarly journals Structural and Biochemical Studies of a Moderately Thermophilic Exonuclease I from Methylocaldum szegediense

PLoS ONE ◽  
2015 ◽  
Vol 10 (2) ◽  
pp. e0117470
Author(s):  
Li Fei ◽  
SiSi Tian ◽  
Ruth Moysey ◽  
Mihaela Misca ◽  
John J. Barker ◽  
...  
Keyword(s):  
Moderately Thermophilic ◽  
Exonuclease I ◽  
Biochemical Studies ◽  
Methylocaldum Szegediense

scholarly journals Growth and Phylogenetic Properties of Novel Bacteria Belonging to the Epsilon Subdivision of the Proteobacteria Enriched fromAlvinella pompejana and Deep-Sea Hydrothermal Vents

2001 ◽  
Vol 67 (10) ◽  
pp. 4566-4572 ◽  
Author(s):  
Barbara J. Campbell ◽  
Christian Jeanthon ◽  
Joel E. Kostka ◽  
George W. Luther ◽  
S. Craig Cary
Keyword(s):  
Deep Sea ◽  
Hydrothermal Vent ◽  
Hydrothermal Vents ◽  
Heterotrophic Bacteria ◽  
Geographic Location ◽  
Optimal Growth ◽  
Small Subunit ◽  
Ribosomal Gene ◽  
Moderately Thermophilic ◽  
Novel Bacteria

ABSTRACT Recent molecular characterizations of microbial communities from deep-sea hydrothermal sites indicate the predominance of bacteria belonging to the epsilon subdivision of Proteobacteria(epsilon Proteobacteria). Here, we report the first enrichments and characterizations of four epsilonProteobacteria that are directly associated withAlvinella pompejana, a deep sea hydrothermal vent polychete, or with hydrothermal vent chimney samples. These novel bacteria were moderately thermophilic sulfur-reducing heterotrophs growing on formate as the energy and carbon source. In addition, two of them (Am-H and Ex-18.2) could grow on sulfur lithoautrotrophically using hydrogen as the electron donor. Optimal growth temperatures of the bacteria ranged from 41 to 45°C. Phylogenetic analysis of the small-subunit ribosomal gene of the two heterotrophic bacteria demonstrated 95% similarity to Sulfurospirillum arcachonense, an epsilon Proteobacteria isolated from an oxidized marine surface sediment. The autotrophic bacteria grouped within a deeply branching clade of the epsilonProteobacteria, to date composed only of uncultured bacteria detected in a sample from a hydrothermal vent along the mid-Atlantic ridge. A molecular survey of various hydrothermal vent environments demonstrated the presence of two of these bacteria (Am-N and Am-H) in more than one geographic location and habitat. These results suggest that certain epsilonProteobacteria likely fill important niches in the environmental habitats of deep-sea hydrothermal vents, where they contribute to overall carbon and sulfur cycling at moderate thermophilic temperatures.

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