The permeability of heterocysts to the gases nitrogen and oxygen

1985 ◽  
Vol 226 (1244) ◽  
pp. 345-366 ◽  
Keyword(s):  
Gas Phase ◽  
Permeability Coefficient ◽  
Doubling Time ◽  
Diffusion Rate ◽  
Pressure Rise ◽  
Gas Diffusion ◽  
Fixation Rate ◽  
Gas Vacuoles ◽  
The Mean ◽  
Anaerobic Environment

Heterocysts of the cyanobacterium Anabaena flos-aquae retina gas vacuoles for several days after differentiation. It is demonstrated that the rate of gas diffusion into a heterocyst that is near an overlying gas phase can be determined approximately from observations on the rate of gas pressure rise required to collapse 50% of its gas vacuoles. The mean permeability coefficient ( α ) of heterocysts O 2 and N 2 was found to be 0.3 s -1 . From this it was calculated that the average permeability ( k ) of the heterocyst surface layer is about 0.4 μm s -1 (within a factor of 2). This is probably within the range that could be provided by a few layers of the 26-C glycolipids in the heterocyst envelope. It is likely, but not proven, that the main route for gas diffusion is through the envelope rather than through the terminal pores of the heterocyst. From measurements of cell nitrogen content (2.7 pg). doubling time (3 days) and heterocyst: vegetative cell ratio (1:24) it was calculated that the average heterocyst fixed 5.9 x 10 -18 mol N 2 s -1 ; this must equal the diffusion rate of N 2 inside the average heterocyst that was 22% below the outside air-saturated concentration. the maximum N 2 fixation rate allowed by the estimated permeability coefficeint would be 2.7 x 10 -17 mol s -1 per heterocyst, slightly greater than the maximum calcualted N 2 fixation rate. The observed permeability coefficient is low enough for the oxygen concentration in the heterocyst to be maintained close to zero by the probable rate of respiration, providing an anaerobic environment for nitrogenase. The rate of O 2 diffusion will limit the N 2 -fixation rate in the dark by limiting the rate at which ATP is supplied by oxidative phosphorylation.

A diffusion kinetic model for epitaxial growth of gallium arsenide layers from the gas phase

1985 ◽  
Vol 50 (8) ◽  
pp. 1774-1783 ◽  
Author(s):  
Emerich Erdös ◽  
Petr Voňka ◽  
Josef Stejskal ◽  
Přemysl Klíma
Keyword(s):  
Boundary Layer ◽  
Gallium Arsenide ◽  
Gas Phase ◽  
Diffusion Rate ◽  
Heterogeneous Reaction ◽  
Leading Edge ◽  
Quantitative Model ◽  
Diffusion Kinetic ◽  
The Mean ◽  
Substrate Size

The growth model of gallium arsenide epitaxial layers has been further extended in two ways. First of all, the location of the substrate in experiments in the reactor has been taken into account, and a general relationship has been derived for dependence of the mean effective thickness of the boundary layer on the distance of the substrate from the leading edge and on the substrate size. Secondly, the effect of rate of the chemical heterogeneous reaction has been investigated in addition to the diffusion rate on the resulting growth velocity of epitaxial gallium arsenide layers. A quantitative model comprising the rates of the both partial processes has been formulated. The theoretical relationships obtained in this way have been confronted with experimental results.

scholarly journals Assessing Early Heterogeneity in Doubling Times of the COVID-19 Epidemic across Prefectures in Mainland China, January–February, 2020

Epidemiologia ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 95-113 ◽  
Author(s):  
Isaac Chun-Hai Fung ◽  
Xiaolu Zhou ◽  
Chi-Ngai Cheung ◽  
Sylvia K. Ofori ◽  
Kamalich Muniz-Rodriguez ◽  
...  
Keyword(s):  
Regression Models ◽  
Doubling Time ◽  
Metropolitan Areas ◽  
Mainland China ◽  
Linear Regression Models ◽  
Case Count ◽  
Daily Time Series ◽  
The Mean ◽  
Doubling Times ◽  
Daily Time

To describe the geographical heterogeneity of COVID-19 across prefectures in mainland China, we estimated doubling times from daily time series of the cumulative case count between 24 January and 24 February 2020. We analyzed the prefecture-level COVID-19 case burden using linear regression models and used the local Moran’s I to test for spatial autocorrelation and clustering. Four hundred prefectures (~98% population) had at least one COVID-19 case and 39 prefectures had zero cases by 24 February 2020. Excluding Wuhan and those prefectures where there was only one case or none, 76 (17.3% of 439) prefectures had an arithmetic mean of the epidemic doubling time <2 d. Low-population prefectures had a higher per capita cumulative incidence than high-population prefectures during the study period. An increase in population size was associated with a very small reduction in the mean doubling time (−0.012, 95% CI, −0.017, −0.006) where the cumulative case count doubled ≥3 times. Spatial analysis revealed high case count clusters in Hubei and Heilongjiang and fast epidemic growth in several metropolitan areas by mid-February 2020. Prefectures in Hubei and neighboring provinces and several metropolitan areas in coastal and northeastern China experienced rapid growth with cumulative case count doubling multiple times with a small mean doubling time.

Pulmonary capillaries are recruited during pulsatile flow

2002 ◽  
Vol 92 (3) ◽  
pp. 1183-1190 ◽  
Author(s):  
Robert G. Presson ◽  
William A. Baumgartner ◽  
Amanda J. Peterson ◽  
Robb W. Glenny ◽  
Wiltz W. Wagner
Keyword(s):  
Pulsatile Flow ◽  
Left Atrial ◽  
Pulmonary Arterial Pressure ◽  
Pressure Rise ◽  
Capillary Recruitment ◽  
Pulmonary Capillaries ◽  
Pulmonary Capillary ◽  
Direct Measurements ◽  
Pulmonary Arterial ◽  
The Mean

Capillaries recruit when pulmonary arterial pressure rises. The duration of increased pressure imposed in such experiments is usually on the order of minutes, although recent work shows that the recruitment response can occur in <4 s. In the present study, we investigate whether the brief pressure rise during cardiac systole can also cause recruitment and whether the recruitment is maintained during diastole. To study these basic aspects of pulmonary capillary hemodynamics, isolated dog lungs were pump perfused alternately by steady flow and pulsatile flow with the mean arterial and left atrial pressures held constant. Several direct measurements of capillary recruitment were made with videomicroscopy. The total number and total length of perfused capillaries increased significantly during pulsatile flow by 94 and 105%, respectively. Of the newly recruited capillaries, 92% were perfused by red blood cells throughout the pulsatile cycle. These data provide the first direct account of how the pulmonary capillaries respond to pulsatile flow by showing that capillaries are recruited during the systolic pulse and that, once open, the capillaries remain open throughout the pulsatile cycle.

scholarly journals Viral Dynamics of Acute HIV-1 Infection

1999 ◽  
Vol 190 (6) ◽  
pp. 841-850 ◽  
Author(s):  
Susan J. Little ◽  
Angela R. McLean ◽  
Celsa A. Spina ◽  
Douglas D. Richman ◽  
Diane V. Havlir
Keyword(s):  
Antiretroviral Therapy ◽  
Hiv Infection ◽  
Doubling Time ◽  
Basic Reproductive Number ◽  
Reproductive Number ◽  
Target Cells ◽  
Viral Dynamics ◽  
Acute Hiv Infection ◽  
Acute Hiv ◽  
The Mean

Viral dynamics were intensively investigated in eight patients with acute HIV infection to define the earliest rates of change in plasma HIV RNA before and after the start of antiretroviral therapy. We report the first estimates of the basic reproductive number (R0), the number of cells infected by the progeny of an infected cell during its lifetime when target cells are not depleted. The mean initial viral doubling time was 10 h, and the peak of viremia occurred 21 d after reported HIV exposure. The spontaneous rate of decline (α) was highly variable among individuals. The phase 1 viral decay rate (δI = 0.3/day) in subjects initiating potent antiretroviral therapy during acute HIV infection was similar to estimates from treated subjects with chronic HIV infection. The doubling time in two subjects who discontinued antiretroviral therapy was almost five times slower than during acute infection. The mean basic reproductive number (R0) of 19.3 during the logarithmic growth phase of primary HIV infection suggested that a vaccine or postexposure prophylaxis of at least 95% efficacy would be needed to extinguish productive viral infection in the absence of drug resistance or viral latency. These measurements provide a basis for comparison of vaccine and other strategies and support the validity of the simian immunodeficiency virus macaque model of acute HIV infection.

scholarly journals Effect of growth regulators on 'Brookfield' apple gas diffusion and metabolism under controlled atmosphere storage

2014 ◽  
Vol 49 (5) ◽  
pp. 323-329 ◽  
Author(s):  
Auri Brackmann ◽  
Fabio Rodrigo Thewes ◽  
Rogerio de Oliveira Anese ◽  
Vanderlei Both
Keyword(s):  
Growth Regulators ◽  
Ethylene Production ◽  
Diffusion Rate ◽  
Gas Diffusion ◽  
Internal Space ◽  
Naphthaleneacetic Acid ◽  
Controlled Atmosphere ◽  
Water Control ◽  
Ethylene Concentration ◽  
Randomized Design

The objective of this work was to evaluate the effect of growth regulators on gas diffusion and on metabolism of 'Brookfield' apple, and to determine their correlation with quality characteristics of fruit stored in controlled atmosphere. A completely randomized design was used with four replicates. After eight months of storage, the effects of water (control), aminoethoxyvinylglycine (AVG), AVG + ethephon, AVG + naphthaleneacetic acid (NAA), ethephon + NAA, sole NAA, 1-MCP, ethylene absorption by potassium permanganate (ABS), AVG + ABS, and of AVG + 1-MCP - applied at different rates and periods - were evaluated on: gas diffusion rate, ethylene production, respiratory rate, internal ethylene concentration, internal CO2 content, mealiness, and intercellular space. Fruit from the control and sole NAA treatments had the highest mealiness occurrence. Growth regulators significantly changed the gaseous diffusion through the pulp of 'Brookfield' apple, mainly in the treatment AVG + ABS, which kept the highest gas diffusion rate. NAA spraying in the field, with or without another growth regulator, increased ripening metabolism by rising ethylene production and respiration rate, and reduced gas diffusion during shelf life. AVG spraying cannot avoid the ethephon effect during the ripening process, and reduces both the internal space and mealiness incidence, but it is not able to induce ethylene production or to increase respiration rates.

scholarly journals Linking lineage and population observables in biological branching processes

2019 ◽  
Author(s):  
Reinaldo García-García ◽  
Arthur Genthon ◽  
David Lacoste
Keyword(s):  
Population Dynamics ◽  
Generation Time ◽  
Doubling Time ◽  
Branching Processes ◽  
Underlying Mechanism ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Division Rate ◽  
Fluctuation Theorems ◽  
The Mean

Using a population dynamics inspired by an ensemble of growing cells, a set of fluctuation theorems linking observables measured at the lineage and population levels are derived. One of these relations implies inequalities comparing the population doubling time with the mean generation time at the lineage or population levels. We argue that testing these inequalities provides useful insights into the underlying mechanism controlling the division rate in such branching processes.

scholarly journals Crystal structure, computational study and Hirshfeld surface analysis of ethyl (2S,3R)-3-(3-amino-1H-1,2,4-triazol-1-yl)-2-hydroxy-3-phenylpropanoate

2019 ◽  
Vol 75 (12) ◽  
pp. 1919-1924
Author(s):  
Abdelkader Ben Ali ◽  
Youness El Bakri ◽  
Chin-Hung Lai ◽  
Jihad Sebhaoui ◽  
Lhoussaine El Ghayati ◽  
...  
Keyword(s):  
Hydrogen Bonds ◽  
Gas Phase ◽  
Surface Analysis ◽  
Computational Study ◽  
Hirshfeld Surface ◽  
Hirshfeld Surface Analysis ◽  
Dft Methods ◽  
Important Interaction ◽  
The Mean ◽  
Title Molecule

In the title molecule, C13H16N4O3, the mean planes of the phenyl and triazole rings are nearly perpendicular to one another as a result of the intramolecular C—H...O and C—H...π(ring) interactions. In the crystal, layers parallel to (101) are generated by O—H...N, N—H...O and N—H...N hydrogen bonds. The layers are connected by inversion-related pairs of C—H...O hydrogen bonds. The experimental molecular structure is close to the gas-phase geometry-optimized structure calculated by DFT methods. Hirshfeld surface analysis indicates that the most important interaction involving hydrogen in the title compound is the H...H contact. The contribution of the H...O, H...N, and H...H contacts are 13.6, 16.1, and 54.6%, respectively.

scholarly journals Central molecular zones in galaxies: 12CO-to-13CO ratios, carbon budget, and X factors

2020 ◽  
Vol 635 ◽  
pp. A131 ◽  
Author(s):  
F. P. Israel
Keyword(s):  
Gas Phase ◽  
Gas Content ◽  
Molecular Gas ◽  
Nearby Galaxy ◽  
Individual Values ◽  
Elemental Abundances ◽  
Hydrogen Column Density ◽  
Galaxy Center ◽  
The Mean ◽  
Co Emission

We present ground-based measurements of 126 nearby galaxy centers in 12CO and 92 in 13CO in various low-J transitions. More than 60 galaxies were measured in at least four lines. The average relative intensities of the first four J 12CO transitions are 1.00:0.92:0.70:0.57. In the first three J transitions, the average 12CO-to-13CO intensity ratios are 13.0, 11.6, and 12.8, with individual values in any transition ranging from 5 to 25. The sizes of central CO concentrations are well defined in maps, but poorly determined by multi-aperture photometry. On average, the J = 1−0 12CO fluxes increase linearly with the size of the observing beam. CO emission covers only a quarter of the HI galaxy disks. Using radiative transfer models (RADEX), we derived model gas parameters. The assumed carbon elemental abundances and carbon gas depletion onto dust are the main causes of uncertainty. The new CO data and published [CI] and [CII] data imply that CO, C°, and C+ each represent about one-third of the gas-phase carbon in the molecular interstellar medium. The mean beam-averaged molecular hydrogen column density is N(H2) = (1.5 ± 0.2)×1021 cm−2. Galaxy center CO-to-H2 conversion factors are typically ten times lower than the “standard” Milky Way X° disk value, with a mean X(CO) = (1.9 ± 0.2)×1019 cm−2/K km s−1 and a dispersion 1.7. The corresponding [CI]-H2 factor is five times higher than X(CO), with X[CI] = (9 ± 2)×1019 cm−2/K km s−1. No unique conversion factor can be determined for [CII]. The low molecular gas content of galaxy centers relative to their CO intensities is explained in roughly equal parts by high central gas-phase carbon abundances, elevated gas temperatures, and large gas velocity dispersions relative to the corresponding values in galaxy disks.

scholarly journals Accumulation of Norfloxacin byBacteroides fragilis

2000 ◽  
Vol 44 (9) ◽  
pp. 2361-2366 ◽  
Author(s):  
Vito Ricci ◽  
Laura J. V. Piddock
Keyword(s):  
Dry Weight ◽  
Aerobic Bacteria ◽  
External Concentration ◽  
Low Concentrations ◽  
Carbonyl Cyanide ◽  
The Mean ◽  
Time Required ◽  
State Concentration ◽  
Anaerobic Environment ◽  
Burk Plot

ABSTRACT The accumulation of norfloxacin by Bacteroides fragilisNCTC 9343 was determined by the modified fluorescence method. The time required to achieve a steady-state concentration (SSC) after allowingB. fragilis to accumulate norfloxacin in an aerobic or an anaerobic environment was ∼2 min; the SSC achieved in air was 90.28 ± 9.32 ng of norfloxacin/mg (dry weight) of cells, and that achieved anaerobically was 98.45 ± 3.7 ng of norfloxacin/mg (dry weight) of cells. Initial rates of accumulation were determined with a range of external concentrations, as up to 8 μg/ml the concentration of norfloxacin accumulated increased proportionally to the external concentration, 12.13 ng/mg (dry weight) of cells per μg of exogenous norfloxacin per ml. At concentrations above 10 μg/ml no increase in the rate of norfloxacin accumulation was observed. From the kinetic data, a Lineweaver-Burk plot calculated a Km of 5.03 μg/ml and a V max of 25.1 ng of norfloxacin/s. With an increase in temperature of between 0 and 30°C, the concentration of norfloxacin accumulated also increased proportionally at 4.722 ng of norfloxacin/mg (dry weight) of cells/°C. At low concentrations of glucose (<0.2%; 11 mM), the concentration of norfloxacin accumulated was decreased. With the addition of 100 μM carbonyl cyanidem-chlorophenylhydrazone (CCCP) the mean SSC of norfloxacin was increased to 116 ± 7.01 ng of norfloxacin/mg (dry weight) of cells; glucose had no significant effect in the presence of CCCP. Magnesium chloride (20 mM) decreased the SSC of norfloxacin to 40.5 ± 3.76 ng of norfloxacin per mg (dry weight) of cells. These data suggest that the mechanism of accumulation of norfloxacin byB. fragilis is similar to that of aerobic bacteria and that the fluoresence procedure is suitable for use with an anaerobic bacterium.

scholarly journals Uptake and reaction of HOBr on frozen and dry NaCl/NaBr surfaces between 253 and 233 K

2002 ◽  
Vol 2 (1) ◽  
pp. 79-91 ◽  
Author(s):  
J. W. Adams ◽  
N. S. Holmes ◽  
J. N. Crowley
Keyword(s):  
Gas Phase ◽  
Relative Concentration ◽  
Gas Diffusion ◽  
Major Product ◽  
The Arctic ◽  
Sea Spray ◽  
Mixed Salt ◽  
Arctic Boundary Layer ◽  
Similar Composition ◽  
Lower Limits

Abstract. The uptake and reaction of HOBr with frozen salt surfaces of variable NaCl / NaBr composition and temperature were investigated with a coated wall flow tube reactor coupled to a mass spectrometer for gas-phase analysis. HOBr is efficiently taken up onto the frozen surfaces at temperatures between 253 and 233 K where it reacts to form the di-halogens BrCl and Br2, which are subsequently released into the gas-phase. The uptake coefficient for HOBr reacting with a frozen, mixed salt surface of similar composition to sea-spray was <approx> 10-2. The relative concentration of BrCl and Br2 released to the gas-phase was found to be strongly dependent on the ratio of Cl- to Br - in the solution prior to freezing / drying. For a mixed salt surface of similar composition to sea-spray the major product at low conversion of surface reactants (i.e. Br - and Cl-) was Br2. Variation of the pH of the NaCl / NaBr solution used to prepare the frozen surfaces was found to have no significant influence on the results. The observations are explained in terms of initial formation of BrCl in a surface reaction of HOBr with Cl-, and conversion of BrCl to Br2 via reaction of surface Br -. Experiments on the uptake and reaction of BrCl with frozen NaCl / NaBr solutions served to confirm this hypothesis. The kinetics and products of the interactions of BrCl, Br2 and Cl2 with frozen salt surfaces were also investigated, and lower limits to the uptake coefficients of > 0.034, >0.025 and >0.028 respectively, were obtained. The uptake and reaction of HOBr on dry salt surfaces was also investigated and the results closely resemble those obtained for frozen surfaces. During the course of this study the gas diffusion coefficients of HOBr in He and H2O were also measured as (273 ± 1) Torr cm2 s-1 and (51 ± 1) Torr cm2 s-1, respectively, at 255 K. The implications of these results for modelling the chemistry of the Arctic boundary layer in springtime are discussed.

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