scholarly journals Two different synchronous spectrofluorimetric approaches for simultaneous determination of febuxostat and ibuprofen

2021 ◽  
Vol 8 (5) ◽  
pp. 210354
Author(s):  
Galal Magdy ◽  
Fathalla Belal ◽  
Ahmed M. Abdel-Megied ◽  
Ahmed F. Abdel Hakiem
Keyword(s):  
Analytical Procedure ◽  
High Sensitivity ◽  
Dosage Forms ◽  
Simultaneous Estimation ◽  
Plasma Samples ◽  
Good Recovery ◽  
Synchronous Spectrofluorimetry ◽  
Tablet Dosage ◽  
First Time

Two green, simple and sensitive synchronous spectrofluorimetric methods were developed for the first time for the simultaneous estimation of febuxostat (FEB) and ibuprofen (IBU). Method I is constant-wavelength synchronous spectrofluorimetry where FEB and IBU were recorded at 329 and 258 nm, respectively, using Δ λ of 40 nm. Method II is constant-energy synchronous spectrofluorimetry using a wavenumber interval of −4000 cm −1 . All measurements were carried out in a borate buffer of pH 7 and distilled water for dilution which increased the methods' greenness. The two methods were rectilinear over concentration ranges of 30.0–700.0 ng ml −1 and 0.5–9.0 µg ml −1 in the first method and 20.0–500.0 ng ml −1 and 0.1–8.0 µg ml −1 in the second method for FEB and IBU, respectively. High sensitivity was attained for the two drugs with limits of quantitations (LODs) down to 0.41 and 5.51 ng ml −1 in the first method and 0.25 and 3.32 ng ml −1 in the second method for FEB and IBU, respectively. Recovery percentages were in the range of 97.3–101.9% after extraction from spiked human plasma samples, demonstrating high bioanalytical applicability. The two methods were further applied to tablet dosage forms with good recovery results. The methods' greenness was assessed according to the analytical Eco-Scale and Green Analytical Procedure Index guidelines.

Novel First Order Derivative UV Spectrophotometric Method for the Determination of Glimepiride in Solid Dosage Forms

2018 ◽  
Vol 8 (3) ◽  
Author(s):  
Santosh Karajgi . ◽  
Sunayana Mali ◽  
Ramaling Kotnal
Keyword(s):  
Dosage Forms ◽  
Order Derivative ◽  
Simultaneous Estimation ◽  
Drug Form ◽  
Solid Dosage Forms ◽  
First Order ◽  
Solid Dosage ◽  
Tablet Dosage ◽  
Guide Lines

Objective: An easy, perfect, specific and exact process has been studied for the simultaneous estimation of Glimepiride pure drug form as well as tablet dosage forms. Methods: A UV method for quantitative evaluation of Glimepiride by first order derivative peak detect method for determination in bulk as well as tablet dosage form is reported as there was a need to expand novel methods to analyze the drug. Results: Glimepiride has absorbance first derivative maxima at 225 nm in Methanol. Glimepiride follows Beer’s law in concentration range of 5-25µg/ml. The outcomes of the study were validated statistically and recovery studies were performed as per ICH guide lines. Conclusion: Thus the projected method can be applied competently for the estimation of Glimepiride in regular analysis in its dosage forms.

DEVELOPMENT AND VALIDATION OF UV SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF PROCHLORPERAZINE MALEATE AND PYRIDOXINE HYDROCHLORIDE IN TABLET DOSAGE FORM BY UV USING MULTI-COMPONENT MODE OF ANALYSIS

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (03) ◽  
pp. 20-25
Author(s):  
G. B Bhagwat ◽  
◽  
S. P Wate ◽  
A. S. Mundhey
Keyword(s):  
Efficient Method ◽  
Dosage Form ◽  
Uv Spectroscopy ◽  
Dosage Forms ◽  
Simultaneous Estimation ◽  
Analysis Method ◽  
Pyridoxine Hydrochloride ◽  
Development And Validation ◽  
Tablet Dosage

Prochlorperazine maleate and pyridoxine hydrochloride in combination are available as tablet dosage forms in the ratio of 1:5. A simple, reproducible and efficient method for the simultaneous determination of prochlorperazine maleate and pyridoxine hydrochloride in tablet dosage form has been developed. The developed method is based on the simultaneous estimation by UV Spectroscopy, using multi-component mode of analysis. In this method 0.3M HCl was used as solvent. Wavelengths selected for estimation of prochlorperazine maleate and pyridoxine hydrochloride in multi-component mode of analysis method were 254.5 nm and 290.5 nm respectively. Both drugs obey Beer-Lambert’s law in concentration range of 1-5 µg/mL (prochlorperazine maleate) and 5-25 µg/mL (pyridoxine hydrochloride). The results of analysis have been validated statistically and by recovery studies.

scholarly journals Simultaneous UV Spectrophotometric Determination of Cetrizine and Dextromethorphan in Tablet Dosage Form

2010 ◽  
Vol 7 (s1) ◽  
pp. S314-S318 ◽  
Author(s):  
R. Vijayalakshmi ◽  
S. Bhargavi ◽  
M. D. Dhanaraju
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Determination ◽  
Dosage Form ◽  
Dosage Forms ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Isobestic Point ◽  
Ich Guidelines ◽  
Tablet Dosage

Two accurate, precise, sensitve and economical procedures for simultaneous estimation of cetrizine and dextromethorphan in tablet dosage forms have been developed. First method employs formation and solving of simultaneous equations using 230 nm and 280 nm as two analytical wavelengths for both drugs in methanol. The second method isQ-analysis based on measurement of absorptivity at 224 nm (as isobestic point) and 280 nm (λmaxof CTZ). Cetrizine and dextromethorphan at their respective λmax280 nm and 230 nm and at 224 nm (isobestic point) shows linearity in a concentration range of 10-30 mcg/mL for both the drugs. The recovery studies confirmed accuracy of the proposed methods and low values of standard deviation confirmed precision of the methods. The methods were validated as per ICH guidelines.

Development of an HPLC-UV Method for Quantification of Stattic

2019 ◽  
Vol 15 (6) ◽  
pp. 568-573
Author(s):  
Soheil Sedaghat ◽  
Ommoleila Molavi ◽  
Akram Faridi ◽  
Ali Shayanfar ◽  
Mohammad Reza Rashidi
Keyword(s):  
High Performance ◽  
Accurate Method ◽  
Plasma Samples ◽  
Promising Target ◽  
Relative Standard ◽  
Dimerization Inhibitor ◽  
Oncogenic Protein ◽  
First Time ◽  
Transcription 3

Background: Signal transducer and activator of transcription 3 (STAT3), an oncogenic protein found constitutively active in many types of human malignancies, is considered to be a promising target for cancer therapy. Objective: In this study for the first time, a simple and accurate method has been developed for the determination of a STAT3 dimerization inhibitor called stattic in aqueous and plasma samples. Methods: A reverse-phase high-performance liquid chromatography (RP-HPLC) composed of C18 column as stationary phase, and the mixture of acetonitrile (60%) and water (40%) as mobile phase with a UV detection at 215 nm were applied for quantification of stattic. The developed method was validated by Food and Drug Administration (FDA) guideline. Results: The method provided a linear range between 1-40 and 2.5-40 µg mL-1 for aqueous and plasma samples, respectively, with a correlation coefficient of 0.999. The accuracy (as recovery) of the developed method was found to be between 95-105% for aqueous medium and 85-115% for plasma samples. The precision (as relative standard deviation) for aqueous and plasma samples was less than 6% and 15%, respectively. The sensitivity of the developed method based on FDA guideline was 1 µg mL-1 for aqueous and 2.5 µg mL-1 for plasma samples. Conclusion: These results show that the established method is a fast and accurate quantification for stattic in aqueous and plasma samples.

scholarly journals A new stability-indicating RP-HPLC method for the determination of dicyclomine hydrochloride and dimethicone combination in tablet dosage forms

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
J. Saroja ◽  
Anantha Lakshmi P.V. ◽  
Y. Rammohan ◽  
D. Divya Reddy
Keyword(s):  
Liquid Chromatography ◽  
Dosage Forms ◽  
Flow Speed ◽  
Hplc Method ◽  
Stability Indicating ◽  
Simultaneous Quantification ◽  
Rp Hplc ◽  
Tablet Formulations ◽  
Tablet Dosage

Abstract Background We describe a “stability-indicating liquid chromatography” technique for the estimation of dimethicone (DEC) and dicyclomine hydrochloride (DEH) in the established tablet formulations. Individual quantification of DEH and DEC was reported. But simultaneous quantification of DEH and DEC was lacking. DEH and DEC were analysed on an “XTerra C18 column (250 mm × 4.6 mm, 5 μm)” with the mobile phase solvent run isocratically with 0.1M K2HPO4-acetonitrile (55:45, v/v) on a flow speed of 1.0 mL/min. Results The chromatographic run period for the DEC and DEH assay was 6.0 min with retention times of 2.134 and 2.865 min, respectively. The method was validated for accuracy (99.453 to 100.417% and 99.703 to 100.303% recovery values for DEH and DEC, respectively), precision (RSV value 0.135% for DEC and 0.171% for DEH), linearity (5–15 μg/mL for DEH and 20–60 μg/mL for DEC), selectivity (no hinderance from excipients) and specificity (no hinderance from degradants) recovery. Conclusion The developed stability-indicating liquid chromatography process was well applied to established tablet formulations.

scholarly journals Simultaneous Estimation of Domperidone and Pantoprazole in Solid Dosage Form by UV Spectrophotometry

2006 ◽  
Vol 3 (3) ◽  
pp. 142-145
Author(s):  
P. Ravi Kumar ◽  
P. Bhanu Prakash ◽  
M. Murali Krishna ◽  
M. Santha Yadav ◽  
C. Asha Deepthi
Keyword(s):  
Simultaneous Equation ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Q Value ◽  
Value Analysis ◽  
Spectrophotometric Methods ◽  
Solid Dosage ◽  
Tablet Dosage

Domperidone is an antiemetic and pantoprazole is an antiulcer drug. Simple, precise, rapid and selective simultaneous equation and Q- analysis UV spectrophotometric methods have been developed for the simultaneous determination of domperidone and pantoprazole from combined tablet dosage forms. The methods involve solving of simultaneous equations and Q-value analysis based on measurement absorptivity at 216, 287 and 290 nm respectively. Linearity lies between 1-15 mcg/mL for domperidone and 0-50 mcg/mL for pantoprazole.

scholarly journals Validated RP-HPLC Method for the Determination of Mycophenolate Mofetil in Tablet Dosage Forms

2013 ◽  
Vol 25 (8) ◽  
pp. 4788-4790
Author(s):  
T. Vijaya Bhaskara Reddy ◽  
G. Ramu ◽  
M. Sravan Kumar ◽  
C. Rambabu
Keyword(s):  
Mycophenolate Mofetil ◽  
Dosage Forms ◽  
Hplc Method ◽  
Rp Hplc ◽  
Tablet Dosage
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