scholarly journals An aptasensor using ceria electrodeposited-screen-printed carbon electrode for detection of epithelial sodium channel protein as a hypertension biomarker

2021 ◽  
Vol 8 (2) ◽  
pp. 202040
Author(s):  
Yeni Wahyuni Hartati ◽  
Dina Ratna Komala ◽  
Diana Hendrati ◽  
Shabarni Gaffar ◽  
Ari Hardianto ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Incubation Time ◽  
Optimization Design ◽  
Electrochemical Impedance ◽  
Transmembrane Protein ◽  
Point Of Care ◽  
Epithelial Sodium Channel ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

Epithelial sodium channel (ENaC) is a transmembrane protein that has an essential role in maintaining the levels of sodium in blood plasma. A person with a family history of hypertension has a high enough amount of ENaC protein in the kidneys or other organs, so that the ENaC protein acts as a marker that a person is susceptible to hypertension. An aptasensor involves aptamers, which are oligonucleotides that function similar to antibodies, as sensing elements. An electrochemical aptasensor for the detection of ENaC was developed using a screen-printed carbon electrode (SPCE) which was modified by electrodeposition of cerium oxide (CeO 2 ). The aptamer immobilization was via the streptavidin–biotin system. The measurement of changes in current of the active redox [Fe(CN) 6 ] 3−/4− was carried out by differential pulse voltammetry. The surfaces of SPCE and SPCE/CeO 2 were characterized using scanning electron microscopy, voltammetry and electrochemical impedance spectroscopy. The Box–Behnken experimental optimization design revealed the streptavidin incubation time, aptamer incubation time and streptavidin concentrations were 30 min, 30 min and 10.8 µg ml −1 , respectively. Various concentrations of ENaC were used to obtain the linearity range of 0.05–3.0 ng ml −1 , and the limits of detection and quantification were 0.012 ng ml −1 and 0.038 ng ml −1 , respectively. This aptasensor method has the potential to measure the ENaC protein levels in urine samples as well as to be a point-of-care device.

Electrochemical Immunosensor Based on Highly Sensitive Amino Functionalized Graphene Nanoplatelets-Modified Screen Printed Carbon Electrode

2020 ◽  
Vol 833 ◽  
pp. 171-175
Author(s):  
Nurul Azurin Badruzaman ◽  
Mohd Azraie Mohd Azmi ◽  
Nur Azura Mohd Said
Keyword(s):  
Incubation Time ◽  
Electrochemical Impedance ◽  
Electrochemical Immunosensor ◽  
Graphene Nanoplatelets ◽  
Carbon Electrode ◽  
Functionalized Graphene ◽  
Working Electrode ◽  
Screen Printed Carbon Electrode ◽  
Rabbit Igg ◽  
Screen Printed

We presented here the development of an immunosensor based on graphene nanoplatelets-modified screen printed carbon electrode (SPCE) with incorporated rabbit IgG on the amino functionalized surface area. In order to improve sensitivity of working electrode, graphene-nanoplatelets solution was fabricated onto surface carbon working electrode. The effect of different (3-aminopropyl) triethoxysilane (APTES) concentrations (0.125, 0.5, 2 and 8% (v/v)) and incubation time of silanization (30, 60 and 90 min) were studied and compared. An electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize our immunosensor based. It is showed that the optimum APTES concentration which provides higher surface coverage and electron transfer rate was 2% concentration (v/v) at 60 min of incubation time. The modified surface was then evaluated by measuring immobilized rabbit IgG via indirect assay using horseradish peroxidase labelled secondary antibody. The optimum detection immobilized IgG was 0.05 mg/mL. These results indicate the potential for amino functionalized graphene nanoplatelets-modified SPCE in detecting protein biomarkers.

scholarly journals An Effective SARS-CoV-2 Electrochemical Biosensor with Modifiable Dual Probes Using a Modified Screen-Printed Carbon Electrode

Micromachines ◽  
2021 ◽  
Vol 12 (10) ◽  
pp. 1171
Author(s):  
Sow-Neng Pang ◽  
Yu-Lun Lin ◽  
Kai-Jie Yu ◽  
Yueh-Er Chiou ◽  
Wai-Hung Leung ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Transmission Rate ◽  
Point Of Care ◽  
Rapid Identification ◽  
Carbon Electrode ◽  
Target Nucleic Acid ◽  
Screen Printed Carbon Electrode ◽  
Polymerase Chain ◽  
User Friendly ◽  
Screen Printed

Due to the severe acute respiratory syndrome coronavirus (SARS-CoV-2, also called coronavirus disease 2019 (COVID-19)) pandemic starting in early 2020, all social activities ceased in order to combat its high transmission rate. Since vaccination combats one aspect for halting the spread of the virus, the biosensor community has looked at another aspect of reducing the burden of the COVID-19 pandemic on society by developing biosensors that incorporate point-of-care (POC) testing and the rapid identification of those affected in order to deploy appropriate measures. In this study, we aim first to propose a screen-printed carbon electrode (SPCE)-based electrochemical biosensor that meets the ASSURED criteria (i.e., affordable, sensitive, specific, user-friendly, rapid, equipment-free, and deliverable) for POC testing, but more importantly, we describe the novelty of our biosensor’s modifiability that uses custom dual probes made from target nucleic acid sequences. Additionally, regarding the sensitivity of the biosensor, the lowest sample concentration was 10 pM (p = 0.0257) without amplification, which might challenge the traditional technique of reverse transcriptase-polymerase chain reaction (RT-PCR). The purpose of this study is to develop a means of diagnostics for the current pandemic as well as to provide an established POC platform for future epidemics.

scholarly journals Non-Enzymatic Impedimetric Sensor Based on 3-Aminophenylboronic Acid Functionalized Screen-Printed Carbon Electrode for Highly Sensitive Glucose Detection

Sensors ◽  
2019 ◽  
Vol 19 (7) ◽  
pp. 1686 ◽  
Author(s):  
Ricardo Adriano Dorledo de Faria ◽  
Hassan Iden ◽  
Luiz Guilherme Dias Heneine ◽  
Tulio Matencio ◽  
Younès Messaddeq
Keyword(s):  
Boronic Acid ◽  
Glucose Sensor ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Phenylboronic Acid ◽  
Carbon Surface ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Highly Sensitive ◽  
Screen Printed

A highly sensitive glucose sensor was prepared by a one-step method using 3-aminophenyl boronic acid as a unit of recognition and a screen-printed carbon electrode (SPCE) as an electrochemical transducer. Scanning Electron Microscopy confirmed the success of the functionalization of the SPCE due to the presence of clusters of boronic acid distributed on the carbon surface. In agreement with the Electrochemical Impedance Spectroscopy (EIS) tests performed before and after the functionalization, Cyclic Voltammetry results indicated that the electroactivity of the electrode decreased 37.9% owing to the presence of the poly phenylboronic acid on the electrode surface. EIS revealed that the sensor was capable to selectively detect glucose at a broad range of concentrations (limit of detection of 8.53 × 10−9 M), not recognizing fructose and sucrose. The device presented a stable impedimetric response when immediately prepared but suffered the influence of the storage time and some interfering species (dopamine, NaCl and animal serum). The response time at optimized conditions was estimated to be equal to 4.0 ± 0.6 s.

scholarly journals Disposable Carbon Dots Modified Screen Printed Carbon Electrode Electrochemical Sensor Strip for Selective Detection of Ferric Ions

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Shao Chien Tan ◽  
Suk Fun Chin ◽  
Suh Cem Pang
Keyword(s):  
Electrochemical Sensor ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Carbon Nanodots ◽  
Selective Detection ◽  
Carbon Electrode ◽  
Ferric Ions ◽  
Detection Range ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

A disposable electrochemical sensor strip based on carbon nanodots (C-Dots) modified screen printed carbon electrode (SPCE) was fabricated for selective detection of ferric ions (Fe3+) in aqueous solution. C-Dots of mean diameters within the range of 1–7 nm were synthesized electrochemically from spent battery carbon rods. The analytical performance of this electrochemical sensor strip was characterized using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The deposition of C-Dots had enhanced the electron-transfer kinetics and current intensity of the SPCE remarkably by 734% as compared to that of unmodified SPCE. Under optimized conditions, the electrochemical sensor strip exhibited a linear detection range of 0.5 to 25.0 ppm Fe3+ with a limit of detection (LOD) of 0.44±0.04 ppm (at S/N ratio = 3). Validation of results by the electrochemical sensor strip was done by comparing analysis results obtained using an Atomic Absorption Spectrometer (AAS).

scholarly journals Electrochemical Determination of Lead & Copper Ions Using Thiolated Calix[4]arene-Modified Screen-Printed Carbon Electrode

Chemosensors ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 157
Author(s):  
Chong Jin Mei ◽  
Nor Azah Yusof ◽  
Shahrul Ainliah Alang Ahmad
Keyword(s):  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Copper Ions ◽  
Current Response ◽  
Carbon Electrode ◽  
Significant Drop ◽  
Screen Printed Carbon Electrode ◽  
Relative Standard ◽  
Competitive Ions ◽  
Screen Printed

This study used a thiolated calix[4]arene derivative modified on gold nanoparticles and a screen-printed carbon electrode (TC4/AuNPs/SPCE) for Pb2+ and Cu2+ determination. The surface of the modified electrode was characterised via Fourier-transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). Differential pulse voltammetry (DPV) was used for the detection of Pb2+ and Cu2+ under optimum conditions. The limit of detection (LOD) for detecting Pb2+ and Cu2+ was 0.7982 × 10−2 ppm and 1.3358 × 10−2 ppm, respectively. Except for Zn2+ and Hg2+, the presence of competitive ions caused little effect on the current response when detecting Pb2+. However, all competitive ions caused a significant drop in the current response when detecting Cu2+, except Ca2+ and Mg2+, suggesting the sensing platform is more selective toward Pb2+ ions rather than copper (Cu2+) ions. The electrochemical sensor demonstrated good reproducibility and excellent stability with a low relative standard deviation (RSD) value in detecting lead and copper ions. Most importantly, the result obtained in the analysis of Pb2+ and Cu2+ had good recovery in river water, demonstrating the applicability of the developed sensor for real samples.

Biological Multilayer Films Assembled by Redox Polymer and HRP on Screen-Printed Carbon Electrode

2012 ◽  
Vol 571 ◽  
pp. 56-59
Author(s):  
Yu Fang Sha ◽  
Mei Zhao ◽  
Ming Quan Yang ◽  
Hai Xin Bai ◽  
Man Zhao
Keyword(s):  
Horseradish Peroxidase ◽  
Electrostatic Interaction ◽  
Electrode Surface ◽  
Multilayer Films ◽  
Carbon Electrode ◽  
Layer By Layer ◽  
Redox Polymer ◽  
Screen Printed Carbon Electrode ◽  
Positively Charged ◽  
Screen Printed

Biological multilayer films of redox polymer and horseradish peroxidase (HRP) were successfully assembled on a screen-printed carbon electrode using layer-by-layer (LBL) assembled method based on the electrostatic interaction. The screen-printed carbon electrode surface was modified by the positively charged redox polymer, and the negatively charged HRP by LBL method.

Screen-printed carbon electrode for the electrochemical detection of conjugated bilirubin

2021 ◽  
pp. 130574
Author(s):  
P.E. Resmi ◽  
Jeethu Raveendran ◽  
P.V. Suneesh ◽  
T. Ramanchandran ◽  
Bipin G Nair ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Conjugated Bilirubin ◽  
Screen Printed

scholarly journals A graphene screen-printed carbon electrode for real-time measurements of unoccupied active sites in a cellulase

2014 ◽  
Vol 447 ◽  
pp. 162-168 ◽  
Author(s):  
Nicolaj Cruys-Bagger ◽  
Hirosuke Tatsumi ◽  
Kim Borch ◽  
Peter Westh
Keyword(s):  
Real Time ◽  
Active Sites ◽  
Carbon Electrode ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed
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