scholarly journals Enhancing the expression of ARK1 genes in poplar leads to multiple branches and transcriptomic changes

2020 ◽  
Vol 7 (9) ◽  
pp. 201201
Author(s):  
Xiaozhen Liu ◽  
Zhiming Zhang ◽  
Wen Bian ◽  
Anan Duan ◽  
Hanyao Zhang
Keyword(s):  
Transgenic Plants ◽  
Quantitative Polymerase Chain Reaction ◽  
Plant Growth And Development ◽  
Expression Levels ◽  
Qpcr Analysis ◽  
Transgenic Lines ◽  
Polymerase Chain ◽  
Transcriptomic Changes ◽  
Insight Into ◽  
Poplar Seedlings

The ARBORKNOX1 ( ARK1 ) gene is an important gene for regulating plant growth and development; however, transcriptomic responses of enhancing expression of ARK1 gene in poplar are poorly investigated. To provide insight into the gene function of the ARK1 gene in poplar, the ARK1 transgenic poplar ‘717' and ‘84 K' lines were obtained, the morphology of transgenic plants was observed, and transcriptome profiles were compared. The results showed that there were multiple branches in ARK1 transgenic seedlings compared with non-transgenic seedlings. The results of transcriptome analysis showed that there were significant differences in transcriptome profiles between the transgenic lines of ‘717' and ‘84 K', and between non-transgenic lines (CK) and transgenic plants. The real-time quantitative polymerase chain reaction (RT-qPCR) analysis confirmed the expression levels of the genes involved in the pathway of zeatin biosynthesis and brassinosteroid biosynthesis. The increase in expression levels of AHP and CYCD3 was related to multiple branches. Enhancing the expression of the ARK1 gene in poplar seedlings leads to multiple branches and transcriptomic changes.

scholarly journals Differential Expression of Benzophenone Synthase and Chalcone Synthase in Hypericum Sampsonii

2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Lili Huang ◽  
Hong Wang ◽  
Hechun Ye ◽  
Zhigao Du ◽  
Yansheng Zhang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Differential Expression ◽  
Chalcone Synthase ◽  
Quantitative Polymerase Chain Reaction ◽  
Transcript Level ◽  
Reproductive Stage ◽  
Vegetative Stage ◽  
Chain Reaction ◽  
Expression Levels ◽  
Polymerase Chain

cDNAs encoding Hypericum sampsonii benzophenone synthase (HsBPS) and chalcone synthase (HsCHS) were isolated and functionally characterized. Differential expressions of HsBPS and HsCHS were monitored using quantitative polymerase chain reaction (PCR). In the vegetative stage, HsBPS was highly expressed in the roots; its transcript level was approx. 100 times higher than that of HsCHS. Relatively high transcript amounts of HsBPS were also detected in older leaves, whereas the youngest leaves contained higher transcript amounts of HsCHS. In the reproductive stage, maximum HsCHS expression was detected in flowers, the transcript level being approx. 5 times higher than that of HsBPS. The inversed situation with a 10-fold difference in the expression levels was observed with fruits. High transcript amounts for both proteins were found in roots.

scholarly journals Supraphysiological Concentrations of Bisphenol A Alter the Expression of Extracellular Vesicle-Enriched miRNAs From Human Primary Granulosa Cells

2019 ◽  
Vol 169 (1) ◽  
pp. 5-13 ◽  
Author(s):  
Rodosthenis S Rodosthenous ◽  
Andrea A Baccarelli ◽  
Abdallah Mansour ◽  
Michal Adir ◽  
Ariel Israel ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Granulosa Cells ◽  
Target Genes ◽  
Quantitative Polymerase Chain Reaction ◽  
Extracellular Vesicle ◽  
Biological Mechanisms ◽  
Expression Levels ◽  
Biologically Relevant ◽  
Reproductive Effects ◽  
Polymerase Chain

Abstract Bisphenol A (BPA) is a widely used chemical that has been detected in follicular fluid and associated with adverse reproductive effects. Granulosa cells have an important role in follicular growth and oocyte maturation, however, little is known about the biological mechanisms of BPA toxicity on human granulosa cells. In this study, we exposed primary granulosa cells to different concentrations of BPA (0, 20, 200, 2000, and 20 000 ng/ml) and used quantitative polymerase chain reaction to measure the expression levels of miRNAs enriched in extracellular vesicles (EV-enriched miRNAs), and cellular levels of selected target genes of differentially expressed EV-enriched miRNAs. We found that exposure to 20 000 ng/ml BPA was associated with decreased levels of EV-miR-27b-3p (FC = 0.58, p = .04) and increased levels of its biologically relevant target genes FADD (FC = 1.22, p = .01), IGF1 (FC = 1.59, p = .06), and PPARG (FC = 1.73, p = .001) as compared with the control. In addition, we observed that under the same exposure conditions, the expression levels of miR-27b-3p in granulosa cells were also downregulated (FC = 0.65, p = .03) as compared with the control. Our findings suggest that both cellular and extracellular changes in gene expression may mediate BPA toxicity in granulosa cells.

Relationship Between MYCN Copy Number and Expression in Rhabdomyosarcomas and Correlation With Adverse Prognosis in the Alveolar Subtype

2005 ◽  
Vol 23 (4) ◽  
pp. 880-888 ◽  
Author(s):  
Daniel Williamson ◽  
Yong-Jie Lu ◽  
Tony Gordon ◽  
Raf Sciot ◽  
Anna Kelsey ◽  
...  
Keyword(s):  
Pediatric Cancer ◽  
Copy Number ◽  
Adverse Outcome ◽  
Quantitative Polymerase Chain Reaction ◽  
High Copy Number ◽  
Molecular Diagnostic ◽  
Chain Reaction ◽  
Clinicopathologic Characteristics ◽  
Expression Levels ◽  
Polymerase Chain

Purpose Amplification of the transcription factor MYCN is an important molecular diagnostic tool in stratifying treatment for neuroblastoma. Increased copy number and overexpression of MYCN in the pediatric cancer rhabdomyosarcoma has been described in a number of small studies with conflicting conclusions about its association with clinicopathologic characteristics. We aimed to study the phenomenon in the largest series to date. Patients and Methods Using quantitative polymerase chain reaction, we measured MYCN copy number and expression levels in rhabdomyosarcoma samples from 113 and 92 individuals with a confirmed diagnosis of rhabdomyosarcoma, respectively. Results Increased copy number of MYCN was found to be a feature of both the embryonal and alveolar subtypes. The copy number and expression levels were significantly greater in the alveolar subtype, although the range of expression in both subtypes spanned several orders of magnitude. MYCN copy number showed a significant correlation with expression in the alveolar subtype; this relationship between copy number and expression could be modeled as a logarithmic function. It is notable that relatively high expression frequently occurred in embryonal rhabdomyosarcoma without high copy number and that low expression was found in some cases with high copy number. In patients with alveolar rhabdomyosarcoma, overexpression (greater than median) or gain of genomic copies of MYCN were significantly associated with adverse outcome. Conclusion MYCN deregulation is a feature of rhabdomyosarcoma tumorigenesis, defines groups of patients with a poor prognosis, and is a potential target for novel therapies.

scholarly journals Transformation With a JrVTE1 Gene for Tocopherol Cyclase Increasing the Content Of Total Tocopherol in Sour Jujube (Zizyphus jujuba Mill.var. spinosus Hu.)

2018 ◽  
Vol 7 (1) ◽  
pp. 13
Author(s):  
Guan Qiuzhu ◽  
Sun Hongyan ◽  
Sun Qingrong
Keyword(s):  
Transgenic Plants ◽  
High Performance ◽  
Total Content ◽  
Total Tocopherol ◽  
Chain Reaction ◽  
Transgenic Lines ◽  
Zizyphus Jujuba ◽  
Tocopherol Cyclase ◽  
Polymerase Chain ◽  
Stems And Leaves

Tocopherol cyclase (VTE1) plays a key role in promoting the production of tocopherol and increasing vitamin E content in plants. JrVTE1 gene isolated and cloned from walnut was transformed into genome of sour jujube (Zizyphus jujuba Mill. var. spinosus Hu.) by Agrobacterium tumefaciens. Putative transgenic lines were checked by polymerase chain reaction (PCR). The content of tocopherol of the transgenic plants were determined by high performance liquid chromatography (HPLC). Compared to the non-transgenic sour jujube plants, the total content of tocopherol in transgenic plants was markedly increased in all tested tissues including the stems and leaves.

Regeneration of vgb-transgenic poplar (Populus alba×P. glandulosa) and the primary observation of growth

2006 ◽  
Vol 3 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Zhang Bing-Yu ◽  
Su Xiao-Hua ◽  
Li Yi-Liang ◽  
Huang Qin-Jun ◽  
Zhang Xiang-Hua ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Blot Hybridization ◽  
Pcr Amplification ◽  
First Year ◽  
Populus Alba ◽  
Rt Pcr ◽  
Dot Blot ◽  
Transgenic Lines ◽  
Polymerase Chain ◽  
Morphological Differences

AbstractIncreasing the growth rate is especially important for low-quality wood applications, so this has become an important goal in poplar breeding. The present study describes the transfer of Vitreoscilla haemoglobin (VHb) gene (vgb) driven by constitutive promoters, by Agrobacterium tumefaciens into poplar (Populus alba×P. glandulosa). From about 450 leaf discs used for transformation, 60 Kan-resistant plants were obtained, and 52 proved to be true transgenic plants. The transgenic nature of these plants was confirmed by polymerase chain reaction (PCR) amplification and Southern dot blot hybridization. The expression of vgb gene in transgenic plants was confirmed by reverse transcriptase-PCR (RT-PCR). The performance of the transgenic lines was evaluated during the first year of growth in a greenhouse. These plants showed no significant stable morphological differences from the untransformed plants. Among them, three vgb-transgenic lines exhibited noticeably higher growth rates in terms of height and diameter.

scholarly journals Propidium monoazide combined with qPCR to differentiate live and dead conidia of Neofabraea actinidiae

2018 ◽  
Vol 71 ◽  
pp. 348
Author(s):  
Lucia Ramos ◽  
I.P. Shamini Pushparajah ◽  
M. Shahjahan Kabir ◽  
Bethan E. Parry ◽  
Kerry R. Everett
Keyword(s):  
Light Emitting Diode ◽  
Quantitative Polymerase Chain Reaction ◽  
Propidium Monoazide ◽  
Chain Reaction ◽  
Light Emitting ◽  
Qpcr Analysis ◽  
Low Concentrations ◽  
Polymerase Chain ◽  
High Concentrations ◽  
Commercial Kit

Neofabraea actinidiae can occasionally cause post-harvest rot in kiwifruit. Quantitative polymerase chain reaction (qPCR) analysis represents a feasible and accurate option for identifying and quantifying this rot but is limited because qPCR results do not differentiate live and dead conidia. Propidium monoazide (PMA) is a photoreactive dye that penetrates into the damaged cell-wall membranes of dead conidia binding to the DNA and thus suppressing its amplification by qPCR. A commercial kit containing PMA was trialled for differentiating between live and dead N. actinidiae conidia. The most suitable conditions were 1 μM PMA with 10 min light emitting diode (LED) exposure, and could clearly distinguish high concentrations of live from similar concentrations of dead conidia when tested separately and as a mixture. Low concentrations of live N. actinidiae conidia could be distinguished from dead ones when tested separately, but not as a mixture. Additional work is needed to optimise the effectiveness of the PMA binding and apply this concept in the orchard.

Evaluating the diagnostic and prognostic value of serum long non-coding RNA CTC-497E21.4 in gastric cancer

2019 ◽  
Vol 57 (7) ◽  
pp. 1063-1072 ◽  
Author(s):  
Wei Zong ◽  
Wei Feng ◽  
Yun Jiang ◽  
Shaoqing Ju ◽  
Ming Cui ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Prognostic Value ◽  
Quantitative Polymerase Chain Reaction ◽  
High Sensitivity ◽  
Expression Levels ◽  
Non Coding Rna ◽  
Potential Biomarker ◽  
Polymerase Chain ◽  
Long Non Coding Rna

Abstract Background Long non-coding RNAs (lncRNAs) have been reported to play a key role in gastric cancer (GC) tumorigenesis. However, the clinical application value of serum lncRNAs in GC has remained largely unknown. We investigated the role of a novel lncRNA named CTC-497E21.4 in the diagnosis and the prognosis of GC. Methods We focused on evaluation of lncRNA CTC-497E21.4 by real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR). The study involved following aspects: (1) confirmation of the higher lncRNA CTC-497E21.4 expression in different types of GC specimens than corresponding controls; (2) evaluation of monitoring tumor dynamics by the serum lncRNA CTC-497E21.4 assay; (3) evaluation of the prognostic value of lncRNA CTC-497E21.4 assay in GC. Results (1) The method of RTFQ-PCR detection of lncRNA CTC-497E21.4 was evaluated to have high sensitivity and specificity. (2) The expression levels of lncRNA CTC-497E21.4 were higher in GC patients compared with corresponding controls (p<0.001), and the combination of serum lncRNA CTC-497E21.4, CEA and CA19-9 could improve diagnostic sensitivity (96.36%). (3) The serum lncRNA CTC-497E21.4 expression levels were lower in postoperative samples than preoperative samples (p=0.0021) and survival curves downloaded from TCGA showed high lncRNA CTC-497E21.4 levels were associated with poor OS of GC (p=0.0351). Conclusions lncRNA CTC-497E21.4 may be a potential biomarker for the diagnosis and the prognosis of GC.

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