scholarly journals Soy protein isolate-carboxymethyl cellulose conjugates with pH sensitivity for sustained avermectin release

2019 ◽  
Vol 6 (7) ◽  
pp. 190685 ◽  
Author(s):  
Long Chen ◽  
Hongjun Zhou ◽  
Li Hao ◽  
Huayao Chen ◽  
Xinhua Zhou
Keyword(s):  
Release Rate ◽  
Soy Protein ◽  
Carboxymethyl Cellulose ◽  
Uv Light ◽  
Average Particle Size ◽  
Soy Protein Isolate ◽  
Weibull Model ◽  
Protein Isolate ◽  
Toxicity Tests ◽  
Average Particle

Carboxymethyl cellulose (CMC) was grafted onto the surface of soy protein isolate (SPI) to obtain soy protein isolate-carboxymethyl cellulose conjugate (SPC). Avermectin (AVM) was hydrophobically encapsulated as a model drug to obtain SPC@AVM. The reaction between SPI and CMC was confirmed by infrared spectroscopy, thermal analysis and SDS-PAGE electrophoresis. The results of scanning electron microscopy showed that the average particle size of the drug-loaded microspheres was 129 nm and the shape of microspheres changed from block to spherical after the addition of AVM. After encapsulation of AVM, the absolute value of zeta potential was greater than 15 mV, which indicated better stability. Compared to AVM solution, SPC@AVM showed more wettability on the leaf surface and the contact angle on the leaves decreased from 71.64° to 57.33°. The maximum liquid holding capacity increased by 41.41%, from 8.85 to 12.52 mg cm −2 , which effectively reduced leaf loss. SPC@AVM also prevented UV photolysis, wherein the half-life was extended from 18 to 68 min when exposed to UV light. Moreover, toxicity tests showed that the encapsulation of AVM was beneficial to retain the insecticidal effect of AVM in the presence of ultraviolet light. The release rate of AVM showed pH responsiveness and the release rate under neutral conditions was faster than acidic and alkaline conditions. Moreover, the process conformed to the Weibull model.

scholarly journals Interaction of Soy Protein Isolate Hydrolysates with Cyanidin-3-O-Glucoside and Its Effect on the In Vitro Antioxidant Capacity of the Complexes under Neutral Condition

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1721
Author(s):  
Yaru Wu ◽  
Zhucheng Yin ◽  
Xuejiao Qie ◽  
Yao Chen ◽  
Maomao Zeng ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Soy Protein ◽  
Average Particle Size ◽  
Soy Protein Isolate ◽  
Surface Hydrophobicity ◽  
Protein Isolate ◽  
Antagonistic Effect ◽  
Masking Effect ◽  
Average Particle

The interaction of soy protein isolate (SPI) and its hydrolysates (SPIHs) with cyanidin-3-O-glucoside (C3G) at pH 7.0 were investigated to clarify the changes in the antioxidant capacity of their complexes. The results of intrinsic fluorescence revealed that C3G binds to SPI/SPIHs mainly through hydrophobic interaction, and the binding affinity of SPI was stronger than that of SPIHs. Circular dichroism and Fourier-transform infrared spectroscopy analyses revealed that the interaction with C3G did not significantly change the secondary structures of SPI/SPIHs, while the surface hydrophobicity and average particle size of proteins decreased. Furthermore, the SPI/SPIHs-C3G interaction induced an antagonistic effect on the antioxidant capacity (ABTS and DPPH) of the complex system, with the masking effect on the ABTS scavenging capacity of the SPIHs-C3G complexes being lower than that of the SPI-C3G complexes. This study contributes to the design and development of functional beverages that are rich in hydrolysates and anthocyanins.

scholarly journals Physical stability and rheological behavior of Pickering emulsions stabilized by protein–polysaccharide hybrid nanoconjugates

2021 ◽  
Vol 10 (1) ◽  
pp. 1293-1305
Author(s):  
See Kiat Wong ◽  
Liang Ee Low ◽  
Janarthanan Supramaniam ◽  
Sivakumar Manickam ◽  
Tin Wui Wong ◽  
...  
Keyword(s):  
Shear Rate ◽  
Average Particle Size ◽  
Soy Protein Isolate ◽  
Physical Stability ◽  
Shear Thickening ◽  
Protein Isolate ◽  
Average Particle ◽  
Pickering Emulsions ◽  
Lower Shear ◽  
Pre Treatment

Abstract This study investigated the emulsifying properties of a protein–polysaccharide hybrid nanoconjugate system comprising cellulose nanocrystals (CNC, 1% w/v) and soy protein isolate at various concentrations (SPI, 1–3% w/v). The average particle size of the nanoconjugate increased, and the zeta potential decreased when 3% (w/v) of SPI was used. The contact angle and thermal stability of CNC improved with the conjugation of SPI. Upon Pickering emulsification, 0.5% (w/v) of CNC–SPI nanoconjugate as particle stabilizer was sufficient to obtain stable emulsions. The CNC–SPI1 formulation (CNC to SPI, 1:1) provided the emulsion with the smallest droplet size and higher emulsifying activity. Intriguingly, ultrasound (US) pre-treatment on nanoconjugates before emulsification significantly reduced the size of the emulsion. The rheological assessment demonstrated that the CNC–SPI-stabilized emulsions exhibit shear thinning behavior at a lower shear rate and shear thickening behavior at a higher shear rate, indicating the interruption of existing attractive interactions between the CNC particles. All emulsions exhibited higher elastic modulus (G′) than viscous modulus (G″), suggesting high viscoelastic properties of the emulsions. This study demonstrates that CNC–SPI nanoconjugate with optimum protein to polysaccharide ratio has great potential as a natural particle stabilizer in food and nutraceutical emulsion applications.

scholarly journals Reducing Fat Content of Fried Potato Pellet Chips Using Carboxymethyl Cellulose and Soy Protein Isolate Solutions as Coating Films

2016 ◽  
Vol 8 (3) ◽  
pp. 162 ◽  
Author(s):  
Malak M. Angor
Keyword(s):  
Soy Protein ◽  
Carboxymethyl Cellulose ◽  
Soy Protein Isolate ◽  
Protein Isolate ◽  
Deep Fat Frying ◽  
Oil Uptake ◽  
Coating Films ◽  
Effective Level ◽  
Sensorial Attributes ◽  
Fat Uptake

<p>The aim of this work was to investigate the influence of different percentages of Carboxymethyl Cellulose (CMC) and Soy Protein Isolate (SPI) as coating material films for potato pellet chips during deep-fat frying on fat uptake, and sensory properties. Coating solutions of 2%, 6%, 10% and 14% w/v were prepared and heated to 90 ºC for 5 minutes then cooled. Samples were dipped in the coating solutions followed by air drying. The treatments were fried and analyzed for fat uptake and moisture retention. Sensorial attributes were also measured. The results obtained have shown that all coating treatments were reduced oil uptake during deep fat frying. The 10% was the most effective level in reducing fat uptake for both coating films. SPI coating films was reducing fat uptake more than CMC for all levels. All samples were found to improve all sensorial features of potato pellet chips compared to control.</p>

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