scholarly journals Goat PDGFRB : unique mRNA expression profile in gonad and significant association between genetic variation and litter size

2019 ◽  
Vol 6 (1) ◽  
pp. 180805 ◽  
Author(s):  
Wenjing Yang ◽  
Hailong Yan ◽  
Ke Wang ◽  
Yang Cui ◽  
Tong Zhou ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Litter Size ◽  
Marker Assisted Selection ◽  
Developmental Stages ◽  
Growth Factor Receptor ◽  
Platelet Derived Growth Factor ◽  
Mrna Expression Profile ◽  
Genetic Breeding ◽  
Potential Association ◽  
Cashmere Goats

β-Type platelet-derived growth factor receptor ( PDGFRB ) is a typical tyrosine kinase, as a candidate gene associated with reproduction. Its main roles include regulation of gonocytes (migration and proliferation) and of the cell cycle. The objectives of this study were to identify mRNA expression of the goat PDGFRB gene, as well as insertion/deletion (indel) variants and their association with litter size in 1122 healthy Shaanbei white cashmere goats. The results revealed that PDGFRB was widely expressed in all tested tissues, and the expression levels in testes at different developmental stages indicated a potential association with the mitosis-to-meiosis transition. Furthermore, the expression of PDGFRB was relatively higher in the ovary tissue of mothers of two lambs compared with mothers of single lamb. These results implied that PDGFRB was related to goat fertility. Meanwhile, two intronic indels, 5 bp ( n = 501) and 10 bp ( n = 1122), were identified. Statistical analysis revealed that only the 10 bp indel was associated with first-born litter size ( n = 1122, p = 6.030 × 10 −5 ), and that individuals of the genotype insertion/deletion had larger litter sizes than those of genotype insertion/insertion. Overall, these results indicated that the 10 bp indel of PDGFRB could be used in marker-assisted selection during goat genetic breeding.

scholarly journals A quantitative RT-PCR study of the mRNA expression profile of the IGF axis during mammary gland development

2004 ◽  
Vol 33 (1) ◽  
pp. 195-207 ◽  
Author(s):  
M Boutinaud ◽  
JH Shand ◽  
MA Park ◽  
K Phillips ◽  
J Beattie ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Mrna Expression ◽  
Expression Profile ◽  
Mammary Gland Development ◽  
Developmental Stages ◽  
Normal Mammary Gland ◽  
Rt Pcr ◽  
Mrna Expression Profile ◽  
Igf I ◽  
Gland Development

We have used quantitative RT-PCR to analyse the mRNA expression profile of the major components of the IGF axis in different stages of murine mammary gland development, including late pregnancy, lactation and involution. We have shown that all the genes studied, IGF-I, IGF-II, IGF receptor (IGFR) and IGF-binding protein (IGFBP)-1 to -6, were expressed in every stage, albeit at greatly differing levels and displaying unique expression profiles between developmental stages. IGF-I was always expressed at significantly higher levels than either IGF-II or IGFR. This suggests that IGF-I may be the more important IGF during mammary morphogenesis. Overall, IGFBP-3 demonstrated the highest level of expression of any of the IGFBP genes throughout all the developmental stages studied. However, within developmental stages, by far the highest level of expression of any of the IGFBPs was that of IGFBP-5 at day 2 of involution; this was almost an order of magnitude higher than any of the other IGFBP levels recorded. This corroborated our previous findings that the levels of IGFBP-5 protein are highly elevated in the involuting mammary gland, and demonstrated that this up-regulation of IGFBP-5 operates at the level of transcriptional control or message stability. Comparison of the expression profile for these different genes would strongly suggest that they are likely to have differential functions throughout mammary gland development, and also highlights potential interactions and co-regulation between different members of this axis. In addition, our results have identified some similarities and differences in the expression of IGFBPs between the mouse mammary epithelial cell line, HC11, and the normal mammary gland which are worthy of study, most notably the differential regulation of IGFBP-2 and the site of expression of IGFBP-4 and -6. Overall, this study has demonstrated the importance and complexity of the IGF axis during mammary gland development and provides a valuable resource for future research in this area.

scholarly journals Polymorphisms within the Boule Gene Detected by Tetra-Primer Amplification Refractory Mutation System PCR (T-ARMS-PCR) are Significantly Associated with Goat Litter Size

Animals ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 910 ◽  
Author(s):  
Song ◽  
Li ◽  
Fei ◽  
Zhang ◽  
Pan ◽  
...  
Keyword(s):  
Litter Size ◽  
Selection Process ◽  
Amplification Refractory Mutation System ◽  
Female Reproduction ◽  
Arms Pcr ◽  
Potential Marker ◽  
Potential Association ◽  
Reproduction Traits ◽  
Mutation System ◽  
Cashmere Goats

As a gene contributing to spermatogenesis, the Boule gene (also called Boll), whose mutations result in azoospermia and sterility of flies and mice, was conserved in reductional maturation divisions. However, in goats, the polymorphisms of Boule, especially with regard to their fundamental roles in female reproduction traits, are still unknown. Therefore, the aims of this study were to detect a potential mutation (rs661484476: g.7254T>C) located in intron 2 of the Boule gene by tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) and to explore its potential association with the litter size of Shaanbei White-Cashmere goats (SBWGs). In this study, g.7254T>C was firstly detected. The TT genotype was the dominant genotype in the single-lamb group, and T was also the dominant allele in all tested groups. Additionally, the detected locus displayed moderate polymorphism with polymorphism information content (PIC) values among all studied goats ranging from 0.303 to 0.344. Notably, according to the χ2 test, the distribution differences for the genotypic frequencies between the single- and multi-lamb groups was significant (p = 0.014). Furthermore, the polymorphisms of the goat Boule gene were significantly associated with the goat litter size in SBWGs (p < 0.05), which indicated that g.7254T>C could be a potential marker in the marker-assisted selection process for potential litter size in goats. These results also indicated that the Boule gene might exercise important functions in female goat reproduction, which provided new insight for female goat breeding and could accelerate the process of goat breeding.

The mRNA expression profile of the goat prion protein testis-specific (PRNT) gene and its associations with litter size

2021 ◽  
Vol 165 ◽  
pp. 69-75
Author(s):  
Yi Bi ◽  
Shaoli Zhang ◽  
Jie Li ◽  
Libang He ◽  
Yuxin Kang ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Prion Protein ◽  
Litter Size ◽  
Expression Profile ◽  
Mrna Expression Profile

Goat CTNNB1: mRNA expression profile of alternative splicing in testis and association analysis with litter size

Gene ◽  
2018 ◽  
Vol 679 ◽  
pp. 297-304 ◽  
Author(s):  
Xuelian Zhang ◽  
Hailong Yan ◽  
Ke Wang ◽  
Tong Zhou ◽  
Mingyue Chen ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
Mrna Expression ◽  
Association Analysis ◽  
Litter Size ◽  
Expression Profile ◽  
Mrna Expression Profile
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