Genetic loss of importin α4 causes abnormal sperm morphology and impacts on male fertility in mouse

2020 ◽  
Vol 34 (12) ◽  
pp. 16224-16242
Author(s):  
Yoichi Miyamoto ◽  
Mitsuho Sasaki ◽  
Haruhiko Miyata ◽  
Yoko Monobe ◽  
Masahiro Nagai ◽  
...  
Keyword(s):  
Male Fertility ◽  
Sperm Morphology ◽  
Abnormal Sperm

scholarly journals The Tug1 Locus is Essential for Male Fertility

2019 ◽  
Author(s):  
Jordan P. Lewandowski ◽  
Gabrijela Dumbović ◽  
Audrey R. Watson ◽  
Taeyoung Hwang ◽  
Emily Jacobs-Palmer ◽  
...  
Keyword(s):  
Male Fertility ◽  
Sperm Morphology ◽  
Sperm Count ◽  
Initial Study ◽  
Genomic Region ◽  
Complete Penetrance ◽  
Abnormal Sperm ◽  
Lncrna Locus ◽  
Mammalian Genomes ◽  
Taurine Upregulated Gene 1

ABSTRACTBackgroundSeveral long noncoding RNAs (lncRNAs) have been shown to function as central components of molecular machines that play fundamental roles in biology. While the number of annotated lncRNAs in mammalian genomes has greatly expanded, their functions remain largely uncharacterized. This is compounded by the fact that identifying lncRNA loci that have robust and reproducible phenotypes when mutated has been a challenge.ResultsWe previously generated a cohort of 20 lncRNA loci knockout mice. Here, we extend our initial study and provide a more detailed analysis of the highly conserved lncRNA locus, Taurine Upregulated Gene 1 (Tug1). We report that Tug1 knockout male mice are sterile with complete penetrance due to a low sperm count and abnormal sperm morphology. Having identified a lncRNA loci with a robust phenotype, we wanted to determine which, if any, potential elements contained in the Tug1 genomic region (DNA, RNA, protein, or the act of transcription) have activity. Using engineered mouse models and cell-based assays, we provide evidence that the Tug1 locus harbors three distinct regulatory activities – two noncoding and one coding: (i) a cis DNA repressor that regulates many neighboring genes, (ii) a lncRNA that can regulate genes by a trans-based function, and finally (iii) Tug1 encodes an evolutionary conserved peptide that when overexpressed impacts mitochondrial membrane potential.ConclusionsOur results reveal an essential role for the Tug1 locus in male fertility and uncover three distinct regulatory activities in the Tug1 locus, thus highlighting the complexity present at lncRNA loci.

Standard Sperm Morphology as a Predictor of Male Fertility Potential

1992 ◽  
Vol 28 (1) ◽  
pp. 39-41 ◽  
Author(s):  
J. H. Check ◽  
A. Bollendorf ◽  
M. Press ◽  
T. Blue
Keyword(s):  
Male Fertility ◽  
Sperm Morphology ◽  
Fertility Potential

Reprint of: Predictive value of abnormal sperm morphology in in vitro fertilization

2019 ◽  
Vol 112 (4) ◽  
pp. e61-e66 ◽  
Author(s):  
Thinus F. Kruger ◽  
Anibal A. Acosta ◽  
Kathryn F. Simmons ◽  
R. James Swanson ◽  
James F. Matta ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Predictive Value ◽  
Sperm Morphology ◽  
Vitro Fertilization ◽  
Abnormal Sperm

scholarly journals A Common Protamine 1 Promoter Polymorphism (-190 C->A) Correlates With Abnormal Sperm Morphology and Increased Protamine P1/P2 Ratio in Infertile Patients

2008 ◽  
Vol 29 (5) ◽  
pp. 540-548 ◽  
Author(s):  
C. Gazquez ◽  
J. Oriola ◽  
S. de Mateo ◽  
J. M. Vidal-Taboada ◽  
J. L. Ballesca ◽  
...  
Keyword(s):  
Sperm Morphology ◽  
Promoter Polymorphism ◽  
Abnormal Sperm ◽  
Infertile Patients ◽  
Protamine 1

2033 GENOME-WIDE ASSOCIATION STUDY IDENTIFIES CANDIDATE GENES FOR ABNORMAL SPERM MORPHOLOGY IN TWO COHORTS OF MEN

2013 ◽  
Vol 189 (4S) ◽  
Author(s):  
James Hotaling ◽  
Gulum Kosova ◽  
Samuel Ohlander ◽  
Craig Niederberger ◽  
Gail S. Prins ◽  
...  
Keyword(s):  
Association Study ◽  
Candidate Genes ◽  
Genome Wide Association Study ◽  
Sperm Morphology ◽  
Genome Wide Association ◽  
Abnormal Sperm ◽  
Genome Wide
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