Transient receptor potential vanilloid 2 activation by focal mechanical stimulation requires interaction with the actin cytoskeleton and enhances growth cone motility

2016 ◽  
Vol 31 (4) ◽  
pp. 1368-1381 ◽  
Author(s):  
Shouta Sugio ◽  
Masami Nagasawa ◽  
Itaru Kojima ◽  
Yasuki Ishizaki ◽  
Koji Shibasaki
Keyword(s):  
Actin Cytoskeleton ◽  
Growth Cone ◽  
Mechanical Stimulation ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Transient Receptor Potential Vanilloid ◽  
Transient Receptor

scholarly journals TRPV2 interacts with actin and reorganizes submembranous actin cytoskeleton

2020 ◽  
Vol 40 (10) ◽  
Author(s):  
Manoj Yadav ◽  
Chandan Goswami
Keyword(s):  
Actin Cytoskeleton ◽  
Transient Receptor Potential ◽  
Neuronal Damage ◽  
Ectopic Expression ◽  
Receptor Potential ◽  
Transient Receptor Potential Vanilloid ◽  
Filamentous Actin ◽  
Peripheral Neuron ◽  
Neurite Initiation ◽  
Transient Receptor

Abstract The understanding of molecules and their role in neurite initiation and/or extension is not only helpful to prevent different neurodegenerative diseases but also can be important in neuronal damage repair. In this work, we explored the role of transient receptor potential vanilloid 2 (TRPV2), a non-selective cation channel in the context of neurite functions. We confirm that functional TRPV2 is endogenously present in F11 cell line, a model system mimicking peripheral neuron. In F11 cells, TRPV2 localizes in specific subcellular regions enriched with filamentous actin, such as in growth cone, filopodia, lamellipodia and in neurites. TRPV2 regulates actin cytoskeleton and also interacts with soluble actin. Ectopic expression of TRPV2-GFP in F11 cell induces more primary and secondary neurites, confirming its role in neurite initiation, extension and branching events. TRPV2-mediated neuritogenesis is dependent on wildtype TRPV2 as cells expressing TRPV2 mutants reveal no neuritogenesis. These findings are relevant to understand the sprouting of new neurites, neuroregeneration and neuronal plasticity at the cellular, subcellular and molecular levels. Such understanding may have further implications in neurodegeneration and peripheral neuropathy.

Sensitization of capsaicin-sensitive lung vagal afferents by anandamide in rats: role of transient receptor potential vanilloid 1 receptors

2009 ◽  
Vol 106 (4) ◽  
pp. 1142-1152 ◽  
Author(s):  
You Shuei Lin ◽  
Ruei-Lung Lin ◽  
Mauo-Ying Bien ◽  
Ching-Yin Ho ◽  
Yu Ru Kou
Keyword(s):  
Receptor Antagonist ◽  
Mechanical Stimulation ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Right Atrial ◽  
Transient Receptor Potential Vanilloid ◽  
Lung Inflation ◽  
Trpv1 Receptors ◽  
Tracheal Pressure ◽  
Transient Receptor

Anandamide (AEA), an arachidonic acid derivative produced during inflammatory conditions, is an endogenous agonist of both transient receptor potential vanilloid 1 (TRPV1) receptors and cannabinoid CB1 receptors. Sensitization of capsaicin-sensitive lung vagal afferent (CSLVA) fibers by chemical mediators is important in the pathogenesis of hyperreactive airway diseases. We investigated the effect of the intravenous infusion of AEA (2 mg·kg−1·ml−1, 0.5 ml/min for 2 min) on the sensitivity of CSLVA fibers to chemical and mechanical stimulation in anesthetized rats. In artificially ventilated rats, AEA infusion only mildly elevated the baseline activity of CSLVA fibers. However, CSLVA fiber responses to right atrial injection of capsaicin, AEA, or adenosine and to lung inflation (tracheal pressure = 30 cmH2O) were all markedly potentiated during AEA infusion, which reverted 20 min after termination of the infusion. The potentiating effect on the sensitivity of CSLVA fibers to adenosine injection or lung inflation was completely blocked by pretreatment with capsazepine (a TRPV1 receptor antagonist) but was unaffected by pretreatment with AM281 (a CB1 receptor antagonist). In spontaneously breathing rats, right atrial injection of adenosine evoked an apneic response that is presumably mediated through CSLVA fibers. Similarly, the adenosine-evoked apneic response was potentiated during AEA infusion, and this potentiating effect was also completely prevented by pretreatment with capsazepine. These results suggest that AEA infusion at the dose tested produces a mild activation of TRPV1 receptors and this nonspecifically increases CSLVA fiber sensitivity to chemical and mechanical stimulation.

Sign in / Sign up

Export Citation Format

Share Document