Adeno‐associated virus (AAV)‐mediated transduction of male germ line stem cells results in transgene transmission after germ cell transplantation

2007 ◽  
Vol 22 (2) ◽  
pp. 374-382 ◽  
Author(s):  
Ali Honaramooz ◽  
Susan Megee ◽  
Wenxian Zeng ◽  
Margret M. Destrempes ◽  
Susan A. Overton ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Germ Line ◽  
Male Germ Line ◽  
Adeno Associated Virus ◽  
Germ Cell Transplantation ◽  
Transgene Transmission

Germ cell transplantation for the propagation of companion animals, non-domestic and endangered species

2007 ◽  
Vol 19 (6) ◽  
pp. 732 ◽  
Author(s):  
I. Dobrinski ◽  
A. J. Travis
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Germ Line ◽  
Genetic Manipulation ◽  
Mammalian Species ◽  
Companion Animals ◽  
Reproductive Potential ◽  
Embryonic Stem ◽  
Germ Cell Transplantation

The transplantation of spermatogonial stem cells between males results in a recipient animal producing spermatozoa carrying a donor’s haplotype. First pioneered in rodents, this technique has now been used in several animal species. Importantly, germ cell transplantation was successful between unrelated, immuno-competent large animals, whereas efficient donor-derived spermatogenesis in rodents requires syngeneic or immuno-compromised recipients. Transplantation requires four steps: recipient preparation, donor cell isolation, transplantation and identifying donor-derived spermatozoa. There are two main applications for this technology. First, genetic manipulation of isolated germ line stem cells and subsequent transplantation will result in production of transgenic spermatozoa. Transgenesis through the male germ line has tremendous potential in species in which embryonic stem cells are not available and somatic cell nuclear transfer and reprogramming pose several problems. Second, spermatogonial stem cell transplantation within or between species offers a means of preserving the reproductive potential of genetically valuable individuals. This might have significance in the captive propagation of non-domestic animals of high conservation value. Transplantation of germ cells is a uniquely valuable approach for the study, preservation and manipulation of male fertility in mammalian species.

scholarly journals Male germ cell transplantation in livestock

2006 ◽  
Vol 18 (2) ◽  
pp. 13 ◽  
Author(s):  
J. R. Hill ◽  
I. Dobrinski
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Large Scale ◽  
Developmental State ◽  
Male Germ Cell ◽  
Seminiferous Tubules ◽  
Cell Transfer ◽  
Donor Sperm ◽  
Germ Cell Transplantation

Male germ cell transplantation is a powerful approach to study the control of spermatogenesis with the ultimate goal to enhance or suppress male fertility. In livestock animals, applications can be expanded to provide an alternative method of transgenesis and an alternative means of artificial insemination (AI). The transplantation technique uses testis stem cells, harvested from the donor animal. These donor stem cells are injected into seminiferous tubules, migrate from the lumen to relocate to the basement membrane and, amazingly, they can retain the capability to produce donor sperm in their new host. Adaptation of the mouse technique for livestock is progressing, with gradual gains in efficiency. Germ cell transfer in goats has produced offspring, but not yet in cattle and pigs. In goats and pigs, the applications of germ cell transplantation are mainly in facilitating transgenic animal production. In cattle, successful male germ cell transfer could create an alternative to AI in areas where it is impractical. Large-scale culture of testis stem cells would enhance the use of elite bulls by providing a renewable source of stem cells for transfer. Although still in a developmental state, germ cell transplantation is an emerging technology with the potential to create new opportunities in livestock production.

scholarly journals New directions in assisted breeding techniques for fish conservation

2020 ◽  
Vol 32 (9) ◽  
pp. 807 ◽  
Author(s):  
Nicola Rivers ◽  
Jonathan Daly ◽  
Peter Temple-Smith
Keyword(s):  
Endangered Species ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Fish Species ◽  
Captive Breeding ◽  
Germ Line ◽  
Management Strategies ◽  
Germ Cell Transplantation ◽  
Spawning Periods ◽  
Breeding Techniques

Fish populations continue to decline globally, signalling the need for new initiatives to conserve endangered species. Over the past two decades, with advances in our understanding of fish germ line biology, new exsitu management strategies for fish genetics and reproduction have focused on the use of germ line cells. The development of germ cell transplantation techniques for the purposes of propagating fish species, most commonly farmed species such as salmonids, has been gaining interest among conservation scientists as a means of regenerating endangered species. Previously, exsitu conservation methods in fish have been restricted to the cryopreservation of gametes or maintaining captive breeding colonies, both of which face significant challenges that have restricted their widespread implementation. However, advances in germ cell transplantation techniques have made its application in endangered species tangible. Using this approach, it is possible to preserve the genetics of fish species at any stage in their reproductive cycle regardless of sexual maturity or the limitations of brief annual spawning periods. Combining cryopreservation and germ cell transplantation will greatly expand our ability to preserve functional genetic samples from threatened species, to secure fish biodiversity and to produce new individuals to enhance or restore native populations.

scholarly journals Aging- and temperature-related activity of spermatogonial stem cells for germ cell transplantation in medaka

2020 ◽  
Vol 155 ◽  
pp. 213-221
Author(s):  
Rungsun Duangkaew ◽  
Fumi Kezuka ◽  
Kensuke Ichida ◽  
Surintorn Boonanuntanasarn ◽  
Goro Yoshizaki
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Spermatogonial Stem Cells ◽  
Related Activity ◽  
Germ Cell Transplantation

scholarly journals 022.Spermatogonial stem cells: from basic research to clinical applications

2004 ◽  
Vol 16 (9) ◽  
pp. 22
Author(s):  
S. Schlatt
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Basic Research ◽  
Testicular Tissue ◽  
Spermatogonial Stem Cells ◽  
Immunodeficient Mice ◽  
Germ Cell Transplantation ◽  
Oncological Patients

The testis contains undifferentiated spermatogonia and is therefore the only adult organ populated with proliferating germline cells. Whereas the biology of these cells is quite well understood in rodents, their modes of mitotic expansion and differentiation are poorly understood in primates. The existence of these cells offers clinically relevant options for preservation and restoration of male fertility. New approaches based on male germ cell transplantation and testicular tissue grafting can be applied to generate a limited number of sperm and could therefore be considered as important new avenues applicable to a variety of disciplines like animal conservation, genetic germline modification or restoration of fertility in oncological patients. In principle, germ cell transplantation presents a removal of the stem cell from the donor's niche and a transfer into the niche of a recipient. Grafting can be considered as a transplantation of the stem cell in conjunction with its niche. Germ cell transplantation of human spermatogonia into mouse testes revealed that the stem cells survive and expand but are not able to differentiate and complete spermatogenesis. We have developed an approach to infuse germ cells into monkey and human testes and showed that germ cell transplantation is feasible as an autologous approach in primates. Furthermore, we applied germ cell transplantation in the monkey model mimicking a gonadal protection strategy for oncological patients. Ectopic xenografting of testicular tissue was applied to generate fertile sperm from a variety of species. Newborn testicular tissue was grafted into the back skin of immunodeficient mice and developed up to qualitatively complete spermatogenesis. The rapid progress in the development of novel experimental strategies to generate sperm from cryopreserved spermatogonial stem cells or immature testicular tissue will lead to many new options for germline manipulation and fertility preservation.

021. Current progress into testis cell transfer between cattle breeds

2005 ◽  
Vol 17 (9) ◽  
pp. 67
Author(s):  
J. Hill ◽  
R. Davey ◽  
M. Herrid ◽  
K. Hutton ◽  
B. Kelley ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Germ Line ◽  
Bos Taurus ◽  
Seminiferous Tubules ◽  
Cell Transfer ◽  
Northern Australia ◽  
Male Germ Line ◽  
Early Results ◽  
Donor Cells

Male germline cell transfer has produced offspring in mice (Brinster and Zimmermann 1994). Recently the first livestock animal, a goat, was produced (Honaramooz et al. 2003), while early results in cattle are promising (Oatley et al. 2002; Izadyar et al. 2003). There is an opportunity to develop this technology for the beef industry by transferring male germ line stem cells between breeds to improve the genetics of extensive Australian beef herds. This project is a part of the CSIRO National Research Flagship program that combines expertise and facilities in divisions with complementary expertise at Monash University and the University of Sydney. The environmental constraints of Northern Australia dictate that Brahman type animals show far better survival than Bos taurus cattle, although the carcass value of Brahmans is lower than Bos taurus animals. Artificial insemination is impractical in Northern Australia and thus we aim to develop testis cell transfer technique in cattle to permit Brahman bulls to deliver semen from elite Bos taurus or composite bulls, thereby significantly increasing the growth rate, yield and meat quality of the northern beef herd. Experiments using cattle were performed to determine the applicability of techniques used in the mouse. Initial proof of concept has been achieved that germ cell transfer can result in the donor cells successfully colonizing areas of recipient testis. The viability of isolated testis cells following short term (24 h) culture has been demonstrated through transfer into recipient calves. We have completed >50 male germ cell transfers into recipient calves, using ultrasonographic guided injection into the rete testis. Success of this procedure has been demonstrated by persistence of PKH26 dyed donor cells in the seminiferous tubules of a majority of recipients >2 months after transfer. These recipient male calves have not been depleted of their endogenous spermatogonial populations and we thus expect the efficiency of the procedure to increase as depletion procedures (ongoing) are established. Concurrent with these developments has been research into large scale culture of male germ line stem cells to provide large numbers of stem cells for transplant. Culture of testis cell suspensions has demonstrated survival of enriched testis cells under varying media and culture conditions. Initial passaging of testis cell colonies has revealed mixed cell populations (immunohistochemistry positive for spermatogonia and somatic cells). Further studies will aim to demonstrate that these cultured donor cells are able to undergo spermatogenesis in the recipient animals.

scholarly journals Spermatogonial stem cells from domestic animals: progress and prospects

Reproduction ◽  
2014 ◽  
Vol 147 (3) ◽  
pp. R65-R74 ◽  
Author(s):  
Yi Zheng ◽  
Yaqing Zhang ◽  
Rongfeng Qu ◽  
Ying He ◽  
Xiue Tian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Germ Cell ◽  
Cell Transplantation ◽  
Pluripotent Stem Cells ◽  
Ethical Issues ◽  
Embryonic Stem ◽  
Domestic Animals ◽  
Spermatogonial Stem Cells ◽  
Germ Cell Transplantation

Spermatogenesis, an elaborate and male-specific process in adult testes by which a number of spermatozoa are produced constantly for male fertility, relies on spermatogonial stem cells (SSCs). As a sub-population of undifferentiated spermatogonia, SSCs are capable of both self-renewal (to maintain sufficient quantities) and differentiation into mature spermatozoa. SSCs are able to convert to pluripotent stem cells during in vitro culture, thus they could function as substitutes for human embryonic stem cells without ethical issues. In addition, this process does not require exogenous transcription factors necessary to produce induced-pluripotent stem cells from somatic cells. Moreover, combining genetic engineering with germ cell transplantation would greatly facilitate the generation of transgenic animals. Since germ cell transplantation into infertile recipient testes was first established in 1994, in vivo and in vitro study and manipulation of SSCs in rodent testes have been progressing at a staggering rate. By contrast, their counterparts in domestic animals, despite the failure to reach a comparable level, still burgeoned and showed striking advances. This review outlines the recent progressions of characterization, isolation, in vitro propagation, and transplantation of spermatogonia/SSCs from domestic animals, thereby shedding light on future exploration of these cells with high value, as well as contributing to the development of reproductive technology for large animals.

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