Carbon monoxide signals via inhibition of cytochrome c oxidase and generation of mitochondrial reactive oxygen species

2007 ◽  
Vol 21 (4) ◽  
pp. 1099-1106 ◽  
Author(s):  
Brian S. Zuckerbraun ◽  
Beek Yoke Chin ◽  
Martin Bilban ◽  
Joana de Costa d'Avila ◽  
Jayashree Rao ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Carbon Monoxide ◽  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Mitochondrial Reactive Oxygen Species

Bioenergetic remodeling during cellular differentiation: changes in cytochrome c oxidase regulation do not affect the metabolic phenotype

2004 ◽  
Vol 82 (3) ◽  
pp. 391-399 ◽  
Author(s):  
Carrie N Lyons ◽  
Scot C Leary ◽  
Christopher D Moyes
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Cytochrome C ◽  
Oxidative Phosphorylation ◽  
Cytochrome C Oxidase ◽  
Oxidative Metabolism ◽  
Specific Activity ◽  
Reactive Oxygen ◽  
Metabolic Phenotype ◽  
Oxygen Species

Myogenesis induces mitochondrial proliferation, a decrease in reactive oxygen species (ROS) production, and an increased reliance upon oxidative phosphorylation. While muscles typically possess 20%–40% excess capacity of cytochrome c oxidase (COX), undifferentiated myoblasts have only 5%–20% of the mitochondrial content of myotubes and muscles. We used two muscle lines (C2C12, Sol8) and 3T3-L1 pre-adipocytes to examine if changes in COX regulation or activity with differentiation cause a shift in metabolic phenotype (i.e., more oxidative, less glycolytic, less ROS). COX activity in vivo can be suppressed by its inhibitor, nitric oxide, or sub-optimal substrate (cytochrome c) concentrations. Inhibition of nitric oxide synthase via L-NAME had no effect on the respiration of adherent undifferentiated cells, although it did stimulate respiration of myoblasts in suspension. While cytochrome c content increased during differentiation, there was no correlation with respiratory rate or reliance on oxidative metabolism. There was no correlation between COX specific activity and oxidative metabolism between cell type or in relation to differentiation. These studies show that, despite the very low activities of COX, undifferentiated myoblasts and pre-adipocytes possess a reserve of COX capacity and changes in COX with differentiation do not trigger the shift in metabolic phenotype.Key words: oxidative phosphorylation, myogenesis, nitric oxide, reactive oxygen species, cytochrome c oxidase.

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