scholarly journals New technologies to introduce a catalytic function into antibodies: A unique human catalytic antibody light chain showing degradation of β-amyloid molecule along with the peptidase activity

2019 ◽  
Vol 1 (2) ◽  
pp. 93-104
Author(s):  
Emi Hifumi ◽  
Hiroaki Taguchi ◽  
Eiichi Toorisaka ◽  
Taizo Uda
Keyword(s):  
Light Chain ◽  
New Technologies ◽  
Catalytic Antibody ◽  
Peptidase Activity ◽  
Catalytic Function ◽  
Β Amyloid

scholarly journals A new algorithm to convert a normal antibody into the corresponding catalytic antibody

2020 ◽  
Vol 6 (13) ◽  
pp. eaay6441
Author(s):  
Emi Hifumi ◽  
Hiroaki Taguchi ◽  
Haruna Tsuda ◽  
Tetsuro Minagawa ◽  
Tamami Nonaka ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Light Chain ◽  
Amino Acid Sequences ◽  
Catalytic Antibodies ◽  
Light Chains ◽  
Catalytic Antibody ◽  
Peptidase Activity ◽  
Peptide Substrate ◽  
Catalytic Function ◽  
Conserved Residue

Over thousands of monoclonal antibodies (mAbs) have been produced so far, and it would be valuable if these mAbs could be directly converted into catalytic antibodies. We have designed a system to realize the above concept by deleting Pro95, a highly conserved residue in CDR-3 of the antibody light chain. The deletion of Pro95 is a key contributor to catalytic function of the light chain. The S35 and S38 light chains have identical amino acid sequences except for Pro95. The former, with Pro95 did not show any catalytic activity, whereas the latter, without Pro95, exhibited peptidase activity. To verify the generality of this finding, we tested another light chain, T99wt, which had Pro95 and showed little catalytic activity. In contrast, a Pro95-deleted mutant enzymatically degraded the peptide substrate and amyloid-beta molecule. These two cases demonstrate the potential for a new method of creating catalytic antibodies from the corresponding mAbs.

Catalytic antibody light chain capable of cleaving a chemokine receptor CCR-5 peptide with a high reaction rate constant

2004 ◽  
Vol 86 (2) ◽  
pp. 217-225 ◽  
Author(s):  
Yukie Mitsuda ◽  
Emi Hifumi ◽  
Kumi Tsuruhata ◽  
Hiroko Fujinami ◽  
Naoki Yamamoto ◽  
...  
Keyword(s):  
Rate Constant ◽  
Chemokine Receptor ◽  
Light Chain ◽  
Reaction Rate ◽  
Reaction Rate Constant ◽  
Catalytic Antibody ◽  
High Reaction ◽  
High Reaction Rate

Targeted destruction of the HIV-1 coat protein gp41 by a catalytic antibody light chain

2002 ◽  
Vol 269 (1-2) ◽  
pp. 283-298 ◽  
Author(s):  
Emi Hifumi ◽  
Yukie Mitsuda ◽  
Kyoko Ohara ◽  
Taizo Uda
Keyword(s):  
Coat Protein ◽  
Light Chain ◽  
Catalytic Antibody ◽  
Protein Gp41 ◽  
Hiv 1

Biochemical features and antiviral activity of a monomeric catalytic antibody light‐chain 23D4 against influenza A virus

2015 ◽  
Vol 29 (6) ◽  
pp. 2347-2358 ◽  
Author(s):  
Emi Hifumi ◽  
Mitsue Arakawa ◽  
Shingo Matsumoto ◽  
Tatsuhiro Yamamoto ◽  
Yoshiki Katayama ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Influenza A Virus ◽  
Light Chain ◽  
Influenza A ◽  
Catalytic Antibody ◽  
Biochemical Features

scholarly journals Biochemical Features of a Catalytic Antibody Light Chain, 22F6, Prepared from Human Lymphocytes

2013 ◽  
Vol 288 (27) ◽  
pp. 19558-19568 ◽  
Author(s):  
Emi Hifumi ◽  
Naoko Fujimoto ◽  
Mitsue Arakawa ◽  
Eri Saito ◽  
Shingo Matsumoto ◽  
...  
Keyword(s):  
Light Chain ◽  
Human Lymphocytes ◽  
Catalytic Antibody ◽  
Biochemical Features

Investigation of active form of catalytic antibody light chain 41S-2-L

2005 ◽  
Vol 96 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Yukie Mitsuda ◽  
Kumi Tsuruhata ◽  
Emi Hifumi ◽  
Masahiro Takagi ◽  
Taizo Uda
Keyword(s):  
Light Chain ◽  
Active Form ◽  
Catalytic Antibody

scholarly journals Sleep, 24-h activity rhythms, and plasma markers of neurodegenerative disease

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Thom S. Lysen ◽  
M. Arfan Ikram ◽  
Mohsen Ghanbari ◽  
Annemarie I. Luik
Keyword(s):  
Neurodegenerative Disease ◽  
Light Chain ◽  
Multiple Testing ◽  
Neuronal Damage ◽  
Activity Rhythm ◽  
Neurofilament Light Chain ◽  
Neurofilament Light ◽  
Activity Rhythms ◽  
Total Tau ◽  
Β Amyloid

AbstractSleep and 24-h activity rhythm disturbances are associated with development of neurodegenerative diseases and related pathophysiological processes in the brain. We determined the cross-sectional relation of sleep and 24-h activity rhythm disturbances with plasma-based biomarkers that might signal neurodegenerative disease, in 4712 middle-aged and elderly non-demented persons. Sleep and activity rhythms were measured using the Pittsburgh Sleep Quality Index and actigraphy. Simoa assays were used to measure plasma levels of neurofilament light chain, and additionally β-amyloid 40, β-amyloid 42, and total-tau. We used linear regression, adjusting for relevant confounders, and corrected for multiple testing. We found no associations of self-rated sleep, actigraphy-estimated sleep and 24-h activity rhythms with neurofilament light chain after confounder adjustment and correction for multiple testing, except for a non-linear association of self-rated time in bed with neurofilament light chain (P = 2.5*10−4). Similarly, we observed no significant associations with β-amyloid 40, β-amyloid 42, and total-tau after multiple testing correction. We conclude that sleep and 24-h activity rhythm disturbances were not consistently associated with neuronal damage as indicated by plasma neurofilament light chain in this population-based sample middle-aged and elderly non-demented persons. Further studies are needed to determine the associations of sleep and 24-h activity rhythm disturbances with NfL-related neuronal damage.

scholarly journals Specific degradation of H. pylori urease by a catalytic antibody light chain

FEBS Journal ◽  
2005 ◽  
Vol 272 (17) ◽  
pp. 4497-4505 ◽  
Author(s):  
Emi Hifumi ◽  
Kenji Hatiuchi ◽  
Takuro Okuda ◽  
Akira Nishizono ◽  
Yoshiko Okamura ◽  
...  
Keyword(s):  
Light Chain ◽  
Catalytic Antibody ◽  
H Pylori ◽  
Specific Degradation
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