scholarly journals First Report of Ovulariopsis on Lupinus havardii

Plant Disease ◽  
2000 ◽  
Vol 84 (4) ◽  
pp. 487-487
Author(s):  
S. P. Fernández-Pavía ◽  
M. Valenzuela-Vázquez
Keyword(s):  
Powdery Mildew ◽  
Infected Plant ◽  
Severe Infection ◽  
Disease Spread ◽  
Vase Life ◽  
Cut Flowers ◽  
Severe Problem ◽  
First Report ◽  
Big Bend ◽  
Close Proximity

In 1998 and 1999 a severe powdery mildew was observed in Las Cruces, NM, on Big Bend Bluebonnets (Lupinus havardii) grown in the greenhouse for cut flowers and vase life studies. An undescribed powdery mildew has been reported on L. havardii (2), but it has been observed only occasionally on leaves and has not cause a severe problem. The powdery mildew observed in Las Cruces began in March and caused severe infection from May through July. The disease spread rapidly due to movement of the pathogen during pruning operations and the close proximity of plants. Plants were heavily infected when no fungicide was applied. Plants were sprayed with the fungicide azoxystrobin, with best control obtained at 687 mg/liter of water. When an infected plant was used as a source of inoculum, disease spread rapidly to healthy plants placed around the infected plant. Infected leaves had chlorotic lesions that later became necrotic. Mycelia, conidiophores, and conidia of the pathogen were observed on leaves and occasionally on petioles and stems. Ellipsoid cylindrical-to-clavate conidia were hyaline, one-celled, and measured 49 to 68.1 μm × 12.2 to 14.7 μm. Conidia were produced on upright, simple conidiophores measuring 171 to 245 μm × 4.9 to 7.3 μm. Fibrosin bodies and cleistothecia were not found. The fungus was identified as an Ovulariopsis sp. (1). This is the first documented report of an Ovulariopsis sp. on L. havardii. References: (1) U. Braun. Beih. Nova Hedwigia 89:1, 1987. (2) W. A. Mackay and T. D. Davis. HortScience 33:348, 1998.

scholarly journals First Report of Powdery Mildew on Potato Caused by Golovinomyces cichoracearum in California

Plant Disease ◽  
2004 ◽  
Vol 88 (3) ◽  
pp. 309-309 ◽  
Author(s):  
M. K. Romberg ◽  
J. J. Nuñez ◽  
J. J. Farrar
Keyword(s):  
New York ◽  
Powdery Mildew ◽  
Standard Error ◽  
Disease Spread ◽  
Disease Symptoms ◽  
First Report ◽  
Erysiphe Cichoracearum ◽  
Golovinomyces Cichoracearum ◽  
Plastic Bag ◽  
Field Report

In October 2003, potato plants in three fields (cv. Desiree, Satina, Midas, and Mondial) in Lancaster, California exhibited symptoms and signs of powdery mildew. Disease symptoms were most severe on cvs. Desiree and Santina. Disease expression was greater along sprinkler lines and in localized areas from which the disease spread to surrounding plants. Severely affected plants began collapsing just prior to water cutoff. Early symptoms comprise small dark areas on the adaxial surface of leaves, along the veins, and at the petioles. Dark lesions consisting of mycelia and conidiophores were also visible on the main stems of affected plants. As the disease progressed, leaves were covered by a gray powdery fungal mass, and older leaves became necrotic. Conidial chains arising from the hyaline, epiphytic mycelia consisted of two to eight conidia. The cylindric to doliform conidia measured 16.8 to 22.8 μm wide (mean = 19.2, standard error = 0.36, N = 30) × 28.8 to 45.6 μm long (mean = 32.4, standard error = 0.75, N = 30). No cleistothecia were observed. Identification of the causal agent as Golovinomyces cichoracearum (synonyms G. orontii and Erysiphe cichoracearum) based on morphology was confirmed by internal transcribed spacer (ITS)-polymerase chain reaction (PCR). Conidia were washed off the affected leaves, concentrated by filtration and centrifugation, and sonicated to release genomic DNA. PCR was performed on the sonicated conidia with primers ITS4 and ITS5 (2), and the resulting amplicon was purified and sequenced. BLAST analysis of the ITS sequence revealed a 99% homology to E. cichoracearum from an Ambrosia sp. (GenBank Accession No. AF011292). Pathogenicity was confirmed on potato seedlings cv. Red La Soda. Inoculations were performed twice on six plants (three pots) each time. A sterile brush was used to transfer conidia from the affected leaves to seedlings consisting of two to three fully expanded leaves. A plastic bag was placed around each pot containing two seedlings for 1 to 2 days and then removed. Noninoculated controls were stroked with a sterile brush, placed in a plastic bag for 1 to 2 days, and kept in the greenhouse on a separate bench. Two control plants were included for each inoculation. Plants were maintained in a greenhouse at approximately 25 to 28°C and 40 to 60% relative humidity. After 7 days, dark spots were visible on the leaves of all inoculated plants, and conidiophores with conidia identical to those of the isolate used as the inoculum source were apparent after 10 days. The controls showed no disease symptoms or signs. To our knowledge, this is the first report of powdery mildew caused by G. cichoracearum on potato in California. The first field report of the disease was from Washington in 1950 (1), with subsequent reports from Utah and Ohio. References: (1) J. D. Menzies. Plant Dis. Rep. 34:140, 1950. (2) T. J. White et al. PCR Protocols. Academic Press, New York, 1990.

scholarly journals Postharvest Characteristics of cut Inflorescences of Lupinus havardii

1995 ◽  
Vol 5 (3) ◽  
pp. 247-249 ◽  
Author(s):  
Tim D. Davis ◽  
Wayne A. Mackay ◽  
Narendra Sankhla
Keyword(s):  
Geographic Range ◽  
Vase Life ◽  
Cut Flowers ◽  
Postharvest Storage ◽  
Breeding Lines ◽  
Silver Thiosulfate ◽  
Big Bend ◽  
Flower Abscission

Big Bend bluebonnet (Lupinus havardii Wats.) is native to a narrow geographic range in southwestern Texas and produces attractive blue inflorescences (racemes) that may be used as cut flowers. Several crops were produced in the greenhouse to determine postharvest-characteristics of the cut inflorescences. Without any postharvest conditioning treatments, the inflorescences held in water had an average vase life of about 7 days. During this period, an average of 13 flowers abscised per inflorescence. When preconditioned for 4 hours in 40 to 160 mg·liter−1 silver thiosulfate (STS), vase life increased to 10 to 12 days and fewer than three flowers abscised per inflorescence. A commercial floral preservative (Oasis) had no effect on flower abscission or vase life of STS-treated inflorescences. Flower abscission and vase life were the same whether STS-treated inflorescences were placed in floral foam moistened with water or in water alone. Storing STS-preconditioned inflorescences in water at 5C for 72 hours did not affect flower abscission or vase life compared to the unstored control. Dry postharvest storage at 5C for 72 hours caused noticeable wilting, but, on dehydration, these inflorescences still had a vase life of about 8 days. Postharvest characteristics of pink-and white-flowered breeding lines were the same as for the blue-flowered line. These results indicate that cut inflorescences of L. havardii have desirable postharvest qualities and can be stored for up to 72 hours without seriously limiting vase life.

scholarly journals First Report of Powdery Mildew on Carrot Caused by Erysiphe heraclei in Michoacan, Mexico

Plant Disease ◽  
2010 ◽  
Vol 94 (4) ◽  
pp. 483-483 ◽  
Author(s):  
G. Rodríguez-Alvarado ◽  
R. Rodríguez-Fernández ◽  
A. Soto-Plancarte ◽  
S. P. Fernández-Pavía
Keyword(s):  
Powdery Mildew ◽  
Morphological Characteristics ◽  
Signs And Symptoms ◽  
Internal Transcribed Spacers ◽  
First Report ◽  
Daucus Carota L ◽  
5.8S Rdna ◽  
Rdna Sequences ◽  
Close Proximity ◽  
Pathogenicity Tests

Carrot (Daucus carota L. subsp. sativus (Hoffm.) Arcang.) is planted as a home-grown vegetable in the central region of Michoacan, Mexico. Powdery mildew was observed on carrot plants cv. Nantesa at several locations near Morelia, Michoacan during March 2009. Affected plants had abundant, white, superficial conidia and mycelium on leaves and stems. All plants at each of five locations surveyed had powdery mildew symptoms with percent foliage coverage ranging from 50 to 80%. Mycelial growth was amphigenous, mainly on the upper leaf surface, covering the whole leaf and with irregular patches on inflorescences and stems. Hyphae were ectophytic with lobed appressoria. Conidiophores presented foot cells 22.5 to 35 (30) × 5.75 to 7 (6.3) μm followed by two cells, one shorter and one longer than the foot cell. Conidia were produced singly, most subcylindric to cylindric, lacked fibrosin bodies, and measured 31.2 to 42 (36.2) × 8.7 to 11.2 (10.5) μm. The teleomorph was not observed. Genomic DNA was extracted from infected leaves; sequences of the internal transcribed spacers (ITS) inclusive of 5.8S rDNA were amplified using previously described primers specific for Erysiphales (3). The ITS sequences shared 100% homology to Erysiphe heraclei specimen VPRI41227 from carrot in Australia (GenBank Accession No. EU371725). On the basis of the morphological characteristics observed and the ITS rDNA sequences, the pathogen was identified as E. heraclei DC. The ITS sequence was deposited in NCBI as Accession No. GU252368. Pathogenicity tests were conducted twice on a total of 10 healthy 8-week-old carrot plants cv. Nantesa. Infected plants were placed in close proximity to healthy plants and maintained in a greenhouse at 27 ± 5°C. Initial signs and symptoms were observed 3 weeks after inoculation and appeared as small, white colonies, which later coalesced and covered most of the foliage. Microscopic examination of the conidia and mycelial morphology matched the originally described pathogen, E. heraclei. Powdery mildew caused by this pathogen has been extensively reported on diverse species and genera of the Apiaceae in Europe and remains one of the most important diseases of carrot (2). The appearance of E. heraclei in diverse regions on a variety of umbelliferous crops indicates that formae speciales have spread, infecting different and specific hosts (1–3). Recently, E. heraclei has been reported on parsley in Puebla, Mexico (4). To our knowledge, this is the first report of E. heraclei causing powdery mildew on carrot in Michoacan, Mexico. This pathogen should be considered as a threat to commercial carrot crops in Mexico. Other crops in the Apiaceae may not be at risk in this area if this powdery mildew is specific for carrots. References: (1) B. J. Aegerter. Page 22 in: Compendium of Umbelliferous Crop Diseases. The American Phytopathological Society, St. Paul, MN, 2002. (2) U. Braun. The Powdery Mildew (Erysiphales) of Europe. Gustav Fischer-Verlag. Jena, Germany, 1995. (3) J. H. Cunnington et al. Australas. Plant Pathol. 32:421, 2003. (4) M. J. Yáñez-Morales et al. Schlechtendalia 19:47, 2009.

scholarly journals First Report of Powdery Mildew of Pumpkin Caused by Golovinomyces cichoracearum in Neelum Valley, Azad Kashmir

Plant Disease ◽  
2012 ◽  
Vol 96 (6) ◽  
pp. 906-906
Author(s):  
I. Mukhtar ◽  
R. Khurram ◽  
A. Hannan ◽  
Z. Hayat
Keyword(s):  
Powdery Mildew ◽  
Infected Plant ◽  
Causal Agent ◽  
Morphological Observation ◽  
Fungal Culture ◽  
Voucher Specimen ◽  
First Report ◽  
Golovinomyces Cichoracearum ◽  
Azad Kashmir ◽  
Basal Septum

During July 2011, a severe powdery mildew outbreak was recorded in pumpkin (Cucurbita moschata Duch.) fields in economically poor areas in the Neelum Valley (Leswa, Mir Pura, Jura, Kundal Shahi, and Bela). Disease symptoms included grayish white circular to irregular patches consisting of epiphytic mycelia and conidia on both surfaces of the leaves of infected vines. Fungal mycelia and conidiophores were also visible in white powdery patches on the main stems, leaves, and petioles of affected plants. Leaves became necrotic with age. Powdery mildew symptoms were more severe on pumpkin vines located in the shade, and severely affected vines collapsed. A voucher specimen (IR00027) has been deposited in First Fungal Culture Bank of the Pakistan (FCBP), Institute of Agriculture Sciences, University of the Punjab, Lahore, Pakistan. For morphological observation, a clear tape strip was used to remove fungal tissue from infected leaves. The tape was mounted on microscope slides with water and examined with a light microscope (2). Conidiophores were unbranched, cylindrical, erect and arose singly on hyphal cells, 110 to 200 × 6 to 12 μm, composed of a foot cell and three to eight barrel-shaped conidia formed in chains with a sinuate edge, followed with a basal septum at the branching point or slightly displaced from the mycelium. Foot cells were 50 to 80 × 6 to 12 μm, and slightly swollen with constriction at the base. Conidia were cylindrical to doliiform, 30 to 50 × 14 to 20 μm and produced in chains. The length/breadth ratio of the conidia was 1.8 to 2.6. Fibrosin bodies were absent in both conidiophores and conidia. No cleistothecia were observed. Identification of the causal agent as Golovinomyces cichoracearum (DC.) V.P. Heluta (synonym Erysiphe cichoracearum) was based on morphology (1). Pathogenicity was determined using field-infected plant leaves transported to a greenhouse. Fresh conidia of field isolates of G. cichoracearum were transferred by a sterile brush from the affected leaves to fully expanded four to five healthy leaves of three 40-day-old vines. For the control, three vines were stroked with a clean sterile brush and control plants were placed 100 m away from the inoculated plants. For disease development 20°C and 80% relative humidity was maintained in the greenhouse. A plastic sheet was placed around each vine for a week and removed later. Inoculated vines developed visible white spots of powdery mildew on the leaves after 10 days in the greenhouse, whereas control plants remained asymptomatic. Fungal colony and conidial morphology on the leaves of inoculated plants were as described above. Previously, G. cichoracearum has been reported on other cucurbits (3) in the plains of Pakistan. To our knowledge, this is the first report of occurrence of G. cichoracearum as a causal agent of powdery mildew on pumpkin in Neelum Valley, Azad Kashmir. References: (1) U. Braun. Beih. Nova Hedwigia 89:1, 1987. (2) J. C. Correll et al. Plant Dis. 71:248, 1987. (3) A. Wahid et al. Pak. J. Agric. Res. 9:209, 1988.

scholarly journals 844 PB 488 POST-HARVEST CHARACTERISTICS OF CUT FLOWER SPIKBS OF LUPINUS HAVARDII

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 554c-554
Author(s):  
Tim D. Davis ◽  
Wayne A. Mackay ◽  
N. Sankhla
Keyword(s):  
Rio Grande ◽  
Geographical Range ◽  
Vase Life ◽  
Blue Flower ◽  
Cut Flower ◽  
Cut Flowers ◽  
Silver Thiosulfate ◽  
Post Harvest ◽  
Big Bend ◽  
Ethylene Inhibitor

Lupinus havardii (Big Bend bluebonnet) is native to a narrow geographical range along the Rio Grande River in southwest Texas and produces attractive blue flower spikes which have potential as cut flowers. Without any post-harvest treatments, these spikes had an average vaselife in water of about 7 d. During this period, an average of about 13 florets were abscised per spike. When preconditioned for 4 h in 40-80 mg/liter silver thiosulfate (STS), vaselife increased to 11 days and only 1-3 florets were abscised per spike. Post-harvest treatment of the spikes with 25-50 mg/liter oxime ether, a new ethylene inhibitor, surprisingly enhanced floret abscission and shortened vaselife. The basis for this response is not clear. Storage of STS-preconditioned spikes in water at 5C for 72 h only decreased vaselife by about one day compared to unstored controls. Dry post-harvest storage at 5C for 72 h caused severe wilting, but upon rehydration these spikes still had a vase/life of about 8 d. These results indicate mat cut flower spikes of L. havardii have good post-harvest qualities and can be stored for up to 3d without seriously limiting vaselife.

scholarly journals First Report of Powdery Mildew of Platanus × acerifolia and P. occidentalis Caused by Erysiphe platani in Greece

Plant Disease ◽  
2015 ◽  
Vol 99 (2) ◽  
pp. 286-286 ◽  
Author(s):  
E. K. Ligoxigakis ◽  
E. A. Markakis ◽  
I. A. Papaioannou ◽  
M. A. Typas
Keyword(s):  
Powdery Mildew ◽  
Infected Plant ◽  
Control Measures ◽  
Universal Primers ◽  
Morphological Description ◽  
Its2 Region ◽  
Conidial Suspension ◽  
Platanus Acerifolia ◽  
First Report ◽  
Young Leaves

London planetrees (Platanus × acerifolia, syn. P. × hispanica), American sycamores (P. occidentalis), and oriental planes (P. orientalis) are widely planted as urban shade trees throughout Greece and many other countries. In June 2012, typical symptoms of a powdery mildew were detected on all sycamores (10 trees) along a central avenue of Heraklion (Crete, Greece), with the disease affecting approximately 80% of the leaves of all infected trees. In August 2013, similar symptoms were observed on 20% of the leaves of all three London planes in a small grove in the Vrysses area of Lasithi (Crete, Greece). In both cases, the disease was severe, with white superficial colonies developing amphigenously on leaves, twigs, floral peduncles, inflorescences, and fruits. The colonies were initially distinct and circular but gradually enlarged and often coalesced to cover the entire leaf blade. Young leaves appeared curled and chlorotic, occasionally leading to defoliation. For the morphological description of the pathogen, samples from seven infected P. occidentalis and three P. × acerifolia trees were microscopically characterized. In all samples, the pathogen's mycelium was branched, septate, and hyaline, with lobed appressoria; conidiophores were erect, cylindrical, unbranched, and consisted of three to four (to five) cells; and conidia were single or in short chains (two to four), ellipsoid or doliiform, with a truncated base and rounded apex. Their dimensions were 24.3 to 48.6 × 15.8 to 27.9 μm (averaging 39.2 × 21.2 μm; n = 100), and their surfaces appeared reticulate. The teleomorph was never observed. Total fungal DNA was extracted from conidia harvested from affected leaves of one infected plant of each of P. occidentalis and P. × acerifolia planes, and the ITS1-5.8S-ITS2 region was PCR-amplified with universal primers 18S-ITS1 and 28S-ITS2 (2) and sequenced (GenBank Accession Nos. KM068123 and KM068124, respectively). A BLASTn search of GenBank revealed 100% identity of both samples to Erysiphe platani strains described on P. orientalis in Greece (JQ365943) and P. occidentalis in Brazil (KF499270). Based on the morphological and molecular analyses, the pathogen was identified as E. platani (Howe) U. Braun & S. Takam. (formerly known as Microsphaera platani Howe) (1). To prove pathogenicity and fulfill Koch's postulates, 10 1-year-old seedlings of each of P. occidentalis and P. × acerifolia hosts were artificially inoculated with conidia obtained from naturally infected plants of the corresponding species, with two methods: (i) five plants of each host were dusted with conidia from diseased leaves, and (ii) the remaining five seedlings of each plane were sprayed with a conidial suspension of the fungus (107 conidia ml−1), while five additional control plants of each species were treated only with sterile distilled water. All plants were maintained in the greenhouse at 25 ± 3°C, with 90% humidity. Powdery mildew symptoms, which appeared 9 and 15 days after inoculation on all dusted and sprayed plants, respectively, were similar to those observed on naturally infected trees, whereas no symptoms were observed on control plants. Although E. platani is known to infect plane species in several parts of the world (1), including oriental planes (P. orientalis and P. orientalis var. cretica) in Greece (3), this is the first report of E. platani causing disease of P. occidentalis and P. × acerifolia in Greece, underlining the need for appropriate control measures to prevent significant losses to the local ornamental industry. References: (1) U. Braun and R. T. A. Cook. Taxonomic Manual of the Erysiphales (Powdery Mildews), CBS Biodiversity Series No. 11. CBS, Utrecht, 2012. (2) I. A. Papaioannou et al. Eur. J. Plant Pathol. 136:577, 2013. (3) D. J. Vakalounakis and E. Klironomou. EPPO Bull. 25:463, 1995.

scholarly journals First Report of Powdery Mildew on Greenhouse Tomatoes Caused by Oidium neolycopersici in Michoacan, Mexico

Plant Disease ◽  
2007 ◽  
Vol 91 (12) ◽  
pp. 1684-1684
Author(s):  
G. Rodríguez-Alvarado ◽  
J. García-López ◽  
R. Rodríguez-Fernández ◽  
S. P. Fernández-Pavía ◽  
E. Garay-Serrano
Keyword(s):  
Powdery Mildew ◽  
Disease Incidence ◽  
Morphological Characteristics ◽  
Severe Infection ◽  
The United States ◽  
Oidium Neolycopersici ◽  
Razor Blade ◽  
First Report ◽  
Tomato Seedlings ◽  
Greenhouse Tomatoes

During June and July of 2007, powdery mildew-infected tomato (Lycopersicum esculentum Mill. cv. Reserve) plants were observed in a commercial greenhouse with an open hydroponic system in Morelia County. Disease incidence increased from 0.5% to more than 90% in 1 month. Infected plants showed leaves with irregular areas of dense, white mycelium covering most of the upper surface. Microscopic analysis showed hyaline, septate hyphae with lobed appressoria. Conidia were ellipsoid to ovoid and 30 to 45 (38) μm × 15 to 20 (16) μm. Conidiophores were erect, 80 to 120 (103) μm, consisted of a foot cell 42 to 67 (56) μm, and two to three short cells. Conidia were produced singly. On the basis of the observed morphological characteristics, the fungus was identified as Oidium neolycopersici L. Kiss (1). Pathogenicity tests were conducted on fourth true-leaf tomato seedlings cv. Reserve under greenhouse conditions (22 ± 5°C). Inoculation was performed by transferring conidia from infected leaves to the leaves of uninfected tomato seedlings with a single-edged razor blade. Powdery mildew symptoms began to develop 7 days after inoculation. Symptoms and morphological characteristics were similar to those observed in the commercial greenhouse. Noninoculated plants remained healthy throughout the experiments. To our knowledge, this is the first report of O. neolycopersici causing powdery mildew on tomato in Michoacan, Mexico. This disease has been reported from Canada, Europe, Japan, the United States (2), and Venezuela (3) on greenhouse and field tomato crops. The observed high incidence and severe infection indicates that this disease may become an important problem in greenhouse tomatoes in Mexico. References: (1) L. Kiss et al. Mycol. Res. 105:684, 2001. (2) L. Kiss et al. Plant Dis. 89:491, 2005. (3) J. O. Montilla et al. Plant Dis. 91:910, 2007.

First Report of Powdery Mildew of Kalanchoe blossfeldiana caused by Sphaerotheca fuliginea (Podosphaera fuliginea) in the Pacific Northwest

2003 ◽  
Vol 4 (1) ◽  
pp. 39
Author(s):  
Dean A. Glawe ◽  
Rita Hummel ◽  
Grace Jack
Keyword(s):  
Powdery Mildew ◽  
Pacific Northwest ◽  
Sphaerotheca Fuliginea ◽  
First Report ◽  
Major Disease ◽  
The Pacific ◽  
Kalanchoe Blossfeldiana ◽  
Extension Center ◽  
Kalanchoë Blossfeldiana

Kalanchoe blossfeldiana Poelln. is a common ornamental houseplant. Although powdery mildew is a major disease of this species, there are no published reports of it in the Pacific Northwest. In August, 2002, powdery mildew was observed on six indoor K. blossfeldiana plants in an office and adjacent laboratory at the Puyallup Research and Extension Center. Accepted for publication 25 March 2003. Published 17 April 2003.

scholarly journals 24-Epibrasinolide Modulates the Vase Life of Lisianthus Cut Flowers by Modulating ACC Oxidase Enzyme Activity and Physiological Responses

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 995
Author(s):  
Mohammad Darvish ◽  
Habib Shirzad ◽  
Mohammadreza Asghari ◽  
Parviz Noruzi ◽  
Abolfazl Alirezalu ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Water Absorption ◽  
Ethylene Production ◽  
Oxidase Activity ◽  
Acc Oxidase ◽  
Postharvest Quality ◽  
Vase Life ◽  
Dependent Manner ◽  
Cut Flowers ◽  
Oxidase Enzyme

Ethylene is the most important factor playing roles in senescence and deterioration of harvested crops including cut flowers. Brassinosteroids (BRs), as natural phytohormones, have been reported to differently modulate ethylene production and related senescence processes in different crops. This study was carried out to determine the effects of different levels of 24-epibrassinolide (EBL) on ACC oxidase enzyme activity, the final enzyme in ethylene biosynthesis pathway, vase life, and senescence rate in lisianthus cut flowers. Harvested flowers were treated with EBL (at 0, 3, 6, and 9 µmol/L) and kept at 25 °C for 15 days. The ACC oxidase activity, water absorption, malondialdehyde (MDA) production and vase solution absorption rates, chlorophyll and anthocyanin contents, and the vase life of the flowers were evaluated during and at the end of storage. EBL at 3 µmol/L significantly (p ≤ 0.01) enhanced the flower vase life by decreasing the ACC oxidase activity, MDA production and senescence rates, and enhancing chlorophyll and anthocyanin biosynthesis and accumulation, relative water content, and vase solution absorption rates. By increasing the concentration, EBL negatively affected the flower vase life and postharvest quality probably via enhancing the ACC oxidase enzyme activity and subsequent ethylene production. EBL at 6 and 9 µmol/L and in a concentration dependent manner, enhanced the ACC oxidase activity and MDA production rate and decreased chlorophyll and anthocyanin accumulation and water absorption rate. The results indicate that the effects of brassinosteroids on ethylene production and physiology of lisianthus cut flowers is highly dose dependent.

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