scholarly journals First Report of Petunia vein clearing virus in Israel

Plant Disease ◽  
2000 ◽  
Vol 84 (2) ◽  
pp. 201-201 ◽  
Author(s):  
A. Gera ◽  
N. Sikron ◽  
J. Cohen ◽  
M. Zeidan
Keyword(s):  
Restriction Analysis ◽  
The United States ◽  
Distinct Pattern ◽  
Nutrient Deficiencies ◽  
Thin Sections ◽  
First Report ◽  
Virus Particles ◽  
Vein Clearing ◽  
Nucleic Acid Extract ◽  
Dna Fragment

Petunia vein clearing virus (PVCV), a possible member of the cauli-movirus group, was detected in several cultivars of vegetatively propagated petunias grown in commercial nurseries in Israel. Symptoms associated with PVCV infection in petunia cv. Chaplin consisted of stunted young shoots, leaf yellowing, and severe vein clearing. Virus incidence within this cultivar was 40 to 60%. Symptoms were most frequently expressed when plants were subjected to temperatures above 20°C and nutrient deficiencies. The virus was transmitted to petunia only by grafting, however, not by aphids or mechanical inoculation. Leaf dip preparations and thin sections of leaf tissue were analyzed by transmission electron microscopy. Spherical virus particles (45 to 50 nm diameter) were observed in samples from symptomatic petunia plants. In immunoelectron microscopy, the virus particles produced a distinct pattern with the specific antiserum against PVCV provided by D. Lesemann (Biologische Bundesanstalt, Braunschweig, Germany). Infection with PVCV also was confirmed by polymerase chain reaction with total nucleic acid extract preparations. Two primer pairs—5′-GAGGTCAGAGCAAGTCAGAGG-3′ (nucleotides 4339 to 4359) and 5′-GTAATGATTTGACTTGTTGAG-3′ (nucleotides 5055 to 5075)—were designed to flank a 736-bp sequence in the RNA-dependent RNA polymerase gene of the PVCV genome (GenBank Accession no. U95208). A DNA fragment of the predicted size was visualized in agarose gels. Authenticity of the amplified DNA fragment was confirmed by restriction analysis. The virus has been detected in the past in Germany (1) and recently in the United States (2). This is the first report of PVCV in commercial nurseries in Israel. References: (1) D. Lesemann and R. Casper. Phytopathology 63:1118, 1973. (2) B. Lockhart and D. Lesemann. Plant Dis. 82:262, 1998.

scholarly journals Improved Detection of Petunia Vein Clearing Caulimovirus

HortScience ◽  
2000 ◽  
Vol 35 (7) ◽  
pp. 1279-1282 ◽  
Author(s):  
M. Zeidan ◽  
Noga Sikron ◽  
J. Cohen ◽  
A. Gera
Keyword(s):  
Electron Microscopy ◽  
Restriction Analysis ◽  
Leaf Tissue ◽  
Polyclonal Antiserum ◽  
Virus Particles ◽  
Vein Clearing ◽  
Transmission Electron ◽  
High Dilutions ◽  
Pair Sequence ◽  
Dna Fragment

Petunia vein clearing virus (PVCV), a possible member of the caulimovirus group, was detected in several cultivars of vegetatively propagated petunias (Petunia ×hybrida Hort. Volm.-Andr.) grown in commercial nurseries. Leaf dip preparations and ultrathin sections of leaf tissue were analyzed by transmission electron microscopy (TEM). Spherical virus particles, 45-50 nm in diameter, were observed in samples taken from symptomatic petunia plants. The virus was purified and a polyclonal antiserum was prepared. In immuno-specific electron microscopy (ISEM), the PVCV antiserum-treated samples reacted with a distinct decoration on the virus suspect particles. A polymerase chain reaction (PCR)-based assay was used to detect PVCV in total nucleic acid extracts derived from infected petunia plants. Two primer pairs were designed to flank a 736-base-pair sequence located in the RNA-dependent RNA polymerase gene of the PVCV genome. A DNA fragment of predicted size was visualized in agarose gels. The authenticity of the amplified DNA fragment was confirmed by restriction analysis and by hybridization with the virus-specific PVCV DNA probe. The virus could be detected efficiently in high dilutions of sap extracted from infected petunia plants.

scholarly journals First Report of ‘Candidatus Liberibacter asiaticus’ Associated with Huanglongbing on Citrus latifolia in Martinique and Guadeloupe, French West Indies

Plant Disease ◽  
2014 ◽  
Vol 98 (5) ◽  
pp. 683-683 ◽  
Author(s):  
G. Cellier ◽  
A. Moreau ◽  
N. Cassam ◽  
B. Hostachy ◽  
P. Ryckewaert ◽  
...  
Keyword(s):  
West Indies ◽  
Plant Protection ◽  
The United States ◽  
Nutrient Deficiencies ◽  
Candidatus Liberibacter Asiaticus ◽  
Protein Subunit ◽  
French West Indies ◽  
First Report ◽  
Candidatus Liberibacter ◽  
Liberibacter Asiaticus

Huanglongbing is an unculturable vascular citrus pathogen transmitted from infected to healthy plants through grafting or by citrus psyllids, Diaphorina citri mainly in Asia and America and Trioza erytreae in Africa. This phloem limited gram-negative bacterium causes dramatic yield losses and is classified into three species based on 16S rDNA sequence analysis (2): (i) ‘Candidatus Liberibacter asiaticus’ (Las), the most epidemiologically active, widespread and heat tolerant species; (ii) ‘Ca. L. africanus’ (Laf), only found in Africa; and (iii) the newly described ‘Ca. L. americanus’ (Lam), which appeared in 2005 in Brazil (5). Considered as a quarantine organism in America and Europe, Las is actively affecting North America and Asia, and research is leading toward psyllid management and resistance breeding. Despite the fact that Reunion Island has successfully controlled Las by introducing a psyllid parasitoid, Tamarixia radiata (1), this strategy was less effective or reproducible within other territories. D. citri was first detected in Guadeloupe in 1998, where the control of the the psyllid population has been effective with T. radiata (3); and was first detected in Martinique in 2012. Following the outbreak in the United States and the Caribbean, and also supported by reports of symptoms in citrus orchards, local National Plant Protection Organizations (NPPO) organized a detection survey across both islands to verify the occurrence of Huanglongbing. Since 2012, 450 sites were prospected each year in Martinique and Guadeloupe, where 20 leaves from 10 to 30 trees were analyzed. DNA extraction was performed (DNeasy Plant Mini Kit, Qiagen) on fresh or dried leaf midribs, along with negative control midribs (Citrus paradisi ‘Star Rubis’) and PCR amplification was done with the species-specific primers A2/J5 (4) and GB1/GB3 (5). Only Las-specific 703-bp amplicons were obtained (n = 43) and 20 were sequenced (Beckman Coulter Genomics, United Kingdom; sequences available through GenBank Accession Nos. KF699074 to KF699093) and blasted against the National Center for Biotechnology Information non-redondant database (NCBI-nr). BLAST analysis revealed 100% identity with the 50S ribosomal protein subunit L1 (rplA) and L10 (rplJ) of ‘Ca. L. asiaticus’ (all strains), and no significant homology to other organisms. Additionally, sequence assembly on a reference genome (NC_012985) showed 100% homology. Huanglongbing was detected in Guadeloupe on March 2012 at Le Moule (East coast) in a Tahiti lime orchard (C. latifolia) and crossed the island in 6 months. Las was detected in Martinique on May 2013 on Tahiti lime (C. latifolia) at Bellefontaine (Northwest) in a private garden and at Le Lorrain (Northeast) in an orchard. Other species from the Rutaceae family were affected by HLB (C. reticulat and C. sinensis) on both islands; however, few of the positive samples showed HLB symptoms (blotchy mottle patterns and green islands on leaves), but presented symptoms similar to nutrient deficiencies. Despite the former presence of T. radiata in Guadeloupe and its detection in Martinique a few weeks after the detection of D. citri, where it had a mean parasitism rate of 70%, an outbreak of HLB spread across both islands. These analyses confirm the presence of HLB in Martinique and Guadeloupe and to our knowledge represent the first report of Las in the French West Indies. Introduction events remain unclear, but this report raises the importance of plant certification, psyllid population control, and surveillance of territories close to the French West Indies, with regards to the risk that HLB presents to citrus production worldwide. References: (1) B. Aubert et al. Fruits. 38, 1983. (2) J. M. Bové. J. Plant Pathol. 88:1, 2006. (3) J. Etienne et al. Fruits. 56:05, 2001. (4) A. Hocquellet et al. Mol. Cell. Probes 13:5, 1999. (5) D. C. Teixeira et al. Mol. Cell. Probes 19:3, 2005.

scholarly journals First Report of Clubroot of Eruca sativa Caused by Plasmodiophora brassicae in Brazil

Plant Disease ◽  
2004 ◽  
Vol 88 (5) ◽  
pp. 573-573 ◽  
Author(s):  
M. L. Paz Lima ◽  
A. C. Café-Filho ◽  
N. L. Nogueira ◽  
M. L. Rossi ◽  
L. R. Schuta
Keyword(s):  
United States ◽  
Plasmodiophora Brassicae ◽  
The United States ◽  
Eruca Sativa ◽  
American Phytopathological Society ◽  
Susceptible Host ◽  
Thin Sections ◽  
First Report ◽  
Western Asia ◽  
Resting Spores

Eruca sativa Mill. (family Brassicaceae), with its origin in western Asia, is a culinary and pharmacological species cultivated in Europe, Brazil, and other countries. In the United States, it is a minor crop known as arugula or roquette. Clubroot on E. sativa has not been reported in Brazil and has been reported once in the United States in 1914 (1,2,3). On several occasions since 2000, stunted and wilted plants (cv. Rúcula Cultivada) were collected from growers' fields and greenhouses that had been direct-seeded in Vargem Bonita, DF (two fields and one greenhouse) and Quatro Barras, PR (two fields). The infected arugula crops were found in areas where other plants from the genus Brassica were traditionally cultivated. Disease incidence in individual fields varied from 20 to 80%. Diseased plants were severely affected with hypertrophic, malformed roots, and root galls resembling Woronin's description (4). Plasmodia and resting spores in thin sections prepared from root galls were observed with compound and electron microscopes. Pathogenicity tests were conducted on arugula and Brassica pekinensis (Lour.) Rupr. (universal host) with inoculum from naturally infected arugula. The soil of potted test plants at the four-to-five-leaf stage was drenched with a suspension of resting spores. Symptoms identical to those observed on the original plants were produced on all inoculated plants 2 to 3 weeks after inoculation. Control plants remained symptomless. The pathogen was positively identified as Plasmodiophora brassicae Wor. with the combination of macroscopic and microscopic symptoms and signs of the disease and pathogen. P. brassicae was first reported in Brazil in 1965 in the state of São Paulo and in the 1980s in Distrito Federal on several members of the Brassicae. To our knowledge, this is the first report of P. brassicae infecting E. sativa in Brazil. Arugula is a susceptible host and should not be planted on P. brassicae-infested land. References: (1) D. Farr et al. Fungi on Plants and Plant Products in the United States. The American Phytopathological Society, St. Paul, MN. 1989. (2) D. F. Farr et al. Fungal Databases. Systematic Botany and Mycology Laboratory, On-line publication. ARS, USDA, 2003. (3) J. S. Karling. The Plasmodiophorales. Published by J. S. karling, NY. 1942. (4) M. S. Woronin. Plasmodiophora brassicae the Cause of Cabbage Hernia. Phytopathological Classics 4. The American Phytopathological Society, Ithaca, NY, 1934.

scholarly journals First Report of Tomato chlorosis virus Infecting Tomato Crops in Brazil

Plant Disease ◽  
2008 ◽  
Vol 92 (12) ◽  
pp. 1709-1709 ◽  
Author(s):  
J. C. Barbosa ◽  
A. P. M. Teixeira ◽  
A. G. Moreira ◽  
L. E. A. Camargo ◽  
A. Bergamin Filho ◽  
...  
Keyword(s):  
Consensus Sequence ◽  
The United States ◽  
Sao Paulo ◽  
São Paulo ◽  
Rt Pcr ◽  
Greenhouse Whitefly ◽  
Thin Sections ◽  
First Report ◽  
Tomato Chlorosis Virus ◽  
Biotype B

During 2006 and 2007 in the region of Sumaré, state of São Paulo, Brazil, surveys were done on tomato (Solanum lycopersicum L.) virus diseases in three open field-grown crops. The data revealed low incidence (0.25 to 3.42%) of randomly distributed plants exhibiting interveinal chlorosis and some necrosis on the basal leaves. Symptoms were only observed on old fruit-bearing plants. Preliminary analysis of thin sections of symptomatic leaves from one plant by transmission electron microscopy revealed the presence of aggregates of thin, flexible, and elongated particles in some phloem vessels, suggesting infection with a member of the genus Crinivirus, family Closteroviridae. Total RNA was extracted separately from leaves of 10 symptomatic plants and used for one-step reverse transcription (RT)-PCR using the HS-11/HS-12 primer pair, which amplifies a fragment of 587 bp from the highly conserved region of the heat shock protein (HSP-70) homolog gene reported for Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus (ToCV) (1). The RT-PCR product was subsequently tested by nested-PCR for single detection of TICV and ToCV using primer pairs TIC-3/TIC-4 and ToC-5/ToC-6, respectively (1). Only one fragment of approximately 463 bp was amplified from 7 of the 10 plants with the primer pair specific for ToCV. No amplification was obtained with the primers specific for TICV. Two amplicons of 463 bp were purified and directly sequenced in both directions. Sequence comparisons of the 463-bp consensus sequence (GenBank Accession No. EU868927) revealed 99% identity with the reported sequence of ToCV from the United States (GenBank Accession No. AY903448) (3). Virus-free adults of Bemisia tabaci biotype B confined on symptomatic tomato leaves for a 24-h acquisition access period were able to transmit the virus to healthy tomato plants, which reproduced the original symptoms on the bottom leaves 65 days after inoculation under greenhouse conditions. Infection from transmission was confirmed by RT-PCR using the HS-11/HS-12 primer pair. In addition to B. tabaci biotype B, the greenhouse whitefly, Trialeurodes vaporariorum, has also been reported as a vector of ToCV, although it is less efficient than the B. tabaci biotype B in transmission of this virus (4). T. vaporariorum, which was previously considered limited to greenhouses, was recently reported in tomato and green bean (Phaseolus vulgaris L.) crops under field conditions in São Paulo State (2). Therefore, it might also contribute to the spread of ToCV in tomato crops in São Paulo. To our knowledge, this is the first report of ToCV in Brazil and South America. References: (1) C. I. Dovas et al. Plant Dis.86:1345, 2002. (2) A. L. Lourenção et al. Neotrop. Entomol. 37:89, 2008. (3) W. M. Wintermantel et al. Arch. Virol. 15:2287, 2005. (4) W. M. Wintermantel and G. C. Wisler. Plant Dis. 90:814, 2006.

scholarly journals First report of a Sheathoid Nematode (Hemicriconemoides litchi) on Longan in Taiwan

Plant Disease ◽  
2021 ◽  
Author(s):  
Che-Chang Liang ◽  
Chin-Wei Chiu ◽  
P. Janet Chen
Keyword(s):  
Tail Length ◽  
Its Region ◽  
The United States ◽  
The Body ◽  
Molecular Characteristics ◽  
Nutrient Deficiencies ◽  
First Report ◽  
Relationship Analysis ◽  
Final Population ◽  
Stylet Length

Longan (Dimocarpus longan Lour.) is a subtropical fruit, widely grown in China, Taiwan, and Southeast Asia. Longan has a high commercial value, US$242,200 of dried longan fruits are exported to the United States from Taiwan every year (Wang et al. 2010). A soil sample from a longan orchard located in Changhua County, Taiwan (24.0162657, 120.529-1457) was collected in June 2019. Sheathoid nematodes were the dominant species in this sample and over 40 adult individuals per 100 g soil were found. In December 2019, sheathoid nematodes were collected again from the same tree and processed for identification by morphological and molecular characteristics. Nematodes were extracted using the modified Baermann funnel method (Wu et al. 2010) for 24 h. The morphometric data from fifteen females with variant tail types were taken. All individuals had a closely sheath, lip region set off with two annuli, stylet frequently slightly dorsally curved with rounded knobs, no vulval flaps, tail narrowing to a broadly rounded terminus, or tapering to a truncate end, anus situated 3-4 annuli posterior to the vulva. The body length = 552 μm (483 to 616 μm), body width at mid-body = 34.02 μm (27.75 to 40.03 μm), a = 15.93 (14.73 to 18.87), b = 4.65 (4.04 to 5.06), V% = 91.57 (86.74 to 92.56), stylet length = 66.66 μm (63.51 to 69.6 μm), tail length = 28.48 μm (23.56 to 37.45 μm), ring number 114 to 130, Rs = 14 to 19, Roes = 22 to 28, RV = 9 to 13, Ran = 5 to 9 and RVan = 3 to 4. Since the stylet length were less than 70μm, they are more fitted to be Hemicriconemoides litchi (Van den Berg et al. 2015). DNA samples extracted using VIAGEN® DirectPCR lysis buffer from single females (n = 10) were processed to amplify the 28S D2-D3 expansion segment and the ITS region using primers sets D2A and D3B, TW81 and AB28, respectively (Van den Berg et al. 2014). The D2-D3 region of the nematodes collected in this study (MT-539384) shared 99% similarity with several H. litchi sequences deposited in the GenBank database (e.g. KP192481, KF856540), and the ITS region (MT556011) also shared 99% similarity with several H. litchi sequences (e.g. KP192482, GQ354786). Therefore, based on morphological, molecular data and phylogenetic relationship analysis (Nguyen et al. 2020) the nematodes from the Longan orchard were determined to be H. litchi. To measure the reproductive factor (Rf), 60 sheathoid nematodes (57 females and three males) recovered from Baermann extraction were inoculated onto root systems of a longan tree in a 15-cm-d pot filled with sterilized soil. One hundred and sixty eight days after inoculation, three 100 cm3 subsamples of soil from the pot were processed as mentioned previously, and the average number was 48/100 cm3 of soil. The final population was approximately 768 nematodes per 1600 cm3 of soil with the Rf value of 12.8 confirming Longan as a host, although no symptoms were observed. The DNA from three individuals in the three subsamples with variant tail types were used to obtain D2-D3 and ITS region sequences, and confirmed the species as H. litchi. Hemicriconemoides spp. is associated with root malformation and nutrient deficiencies on agricultural fruit trees (Milne et al. 1971; McSorley et al. 1980); however, aboveground evidence of damage may not become immediately obvious and the importance of sheathoid nematodes is easily overlooked (Chen et al. 2011). This is the first report of H. litchi as a parasite of longan tree in Taiwan.

scholarly journals First Report of Passiflora latent virus in Banana Passionfruit (Passiflora tarminiana) in New Zealand

Plant Disease ◽  
2008 ◽  
Vol 92 (3) ◽  
pp. 486-486 ◽  
Author(s):  
J. Tang ◽  
G. R. G. Clover ◽  
B. J. R. Alexander ◽  
B. D. Quinn
Keyword(s):  
Electron Microscopy ◽  
New Zealand ◽  
The United States ◽  
Polyclonal Antiserum ◽  
Latent Virus ◽  
Control Measures ◽  
Chenopodium Amaranticolor ◽  
Important Species ◽  
First Report ◽  
Virus Particles

Passiflora latent virus (PLV) naturally infects cultivated and wild Passiflora species in Australia, Germany, Israel and the United States (1–3). In March 2004, chlorotic lesions were observed on leaves of three vines of Passiflora tarminiana on one site in Auckland, New Zealand. Chenopodium amaranticolor and C. quinoa inoculated with sap from symptomatic leaves developed chlorotic local spots, followed by systemic leaf chlorosis and necrosis. Local symptoms appeared more quickly on C. quinoa (12 days) than on C. amaranticolor (20 days). No symptoms were observed on inoculated plants of Nicotiana benthamiana, N. clevelandii, N. occidentalis, N. tabacum, or Phaseolus vulgaris. Electron microscopy of crude sap preparations from infected C. quinoa, C. amaranticolor, N. occidentalis, and P. tarminiana showed flexuous, filamentous virus particles approximately 650 nm long. Plants of P. tarminiana and the three inoculated indicator species containing virus particles tested positive by PLV polyclonal antiserum in double-antibody sandwich (DAS)-ELISA (DSMZ, Braunschweig, Germany) and immunosorbent electron microscopy (Stephan Winter, DSMZ, personal communication). Nucleic acid was extracted from leaves of plants of each of the four viruliferous species with an RNeasy Plant Mini Kit (Qiagen, Doncaster, Australia) and then used in reverse transcription (RT)-PCR tests with novel forward (5′-CGAGACACACGCAAACGAA-3′) and reverse (5′-CAGCAAAGCAAAGACACGA-3′) primers specific to a 523-bp fragment of the PLV polyprotein. PCR products of the expected size were obtained, and an amplicon from P. tarminiana was directly sequenced (GenBank Accession No. EU257510). A BLAST search in GenBank showed 94% nucleotide sequence identity with a PLV isolate from Israel (GenBank Accession No. DQ455582). To our knowledge, this is the first finding of PLV in P. tarminiana and the first report of the virus in New Zealand. Chenopodium spp. have been reported previously as experimental hosts (2,3), and this study revealed that N. occidentalis also can be infected latently with PLV. P. tarminiana is a weed in New Zealand and subject to active control measures to manage the species. Economically important species such as P. edulis and P. ligularis are potentially susceptible to the virus. These species are not grown commercially in the surrounding area but are common in domestic Auckland gardens. Infected vines were removed from the site and destroyed, and symptomatic vines have not been observed at other sites. References: (1) R. D. Pares et al. Plant Dis. 81:348, 1997. (2) S. Spiegel et al. Arch. Virol. 152:181, 2007. (3) A. A. Stihll et al. Plant Dis. 76:843, 1992.

First Report of Turnip vein-clearing virus in Garlic Mustard (Alliaria petiolata) in the United States

2014 ◽  
Vol 15 (4) ◽  
pp. 157-158
Author(s):  
Ben Lockhart ◽  
Dimitre Mollov ◽  
Shauna Mason ◽  
Sara Bratsch
Keyword(s):  
United States ◽  
The United States ◽  
Alliaria Petiolata ◽  
Garlic Mustard ◽  
First Report ◽  
Vein Clearing

The objective of the study was to establish the identity of a tobamo-like virus occurring naturally in garlic mustard in Minnesota, and to determine its role in the etiology of the disease. This is the first report of TVCV infection in garlic mustard. Accepted 18 September 2014. Published 14 October 2014.

scholarly journals First Report of Apricot vein clearing-associated virus Infecting flowering apricot (Prunus mume) in the United States

Plant Disease ◽  
2021 ◽  
Author(s):  
Maher Al Rwahnih ◽  
Nourolah Soltani ◽  
Reid Soltero Brisbane ◽  
Tongyan Tian ◽  
Deborah Anne Golino
Keyword(s):  
High Throughput Sequencing ◽  
De Novo ◽  
The United States ◽  
Cdna Libraries ◽  
Mechanical Transmission ◽  
Viral Agent ◽  
Sequencing Analysis ◽  
First Report ◽  
Vein Clearing ◽  
Biological Studies

Apricot vein clearing-associated virus is the type species of genus Prunevirus, family Betaflexiviridae. The virus was first discovered from an Italian apricot tree (Prunus armeniaca) showing leaf vein clearing and mottling symptoms (Elbeaino et al. 2014). Since then, apricot vein clearing-associated virus (AVCaV) has been reported in symptomatic and asymptomatic plants from other countries (Marais et al. 2015; Kinoti et al. 2017; Kubaa et al. 2014). In 2018, a domestic selection of a flowering apricot (P. mume cv. Peggy Clarke) (PC01) with no discernible foliar virus-like symptoms was received for inclusion in the Foundation Plant Services (UC-Davis) collection. The plant originated from a private Prunus collection located in California. Total nucleic acids (TNA) were isolated from PC01 leaves using MagMax Plant RNA Isolation Kit (Thermo Fisher Scientific). The TNA were analyzed for a panel of 15 Prunus-infecting viruses by reverse-transcription quantitative PCR (RT-qPCR) (Diaz-Lara et al. 2020). In addition, to screen for sap-transmissible viruses, young leaves of PC01 were homogenized in inoculation buffer and were rubbed onto leaves of herbaceous indicator plants, Chenopodium amaranticolor, C. quinoa, Cucumis sativus, and Nicotiana clevelandii (Rowhani et al. 2005). The source PC01 tested negative for the 15 screened viruses. Interestingly, vein clearing symptoms were observed on leaves of C. quinoa and C. amaranticolor plants (Figure S1). These results suggested the presence of a mechanically transmissible virus in PC01. To determine the identity of mechanically transmissible viral agent, symptomatic C. quinoa and PC01 plant were advanced for high throughput sequencing analysis. Aliquots of TNA from PC01 and C. quinoa were rRNA-depleted and used for cDNA library preparation with TruSeq Stranded Total RNA kit (Illumina). The raw reads were trimmed, de novo assembled, and subsequently were annotated using tBLASTx algorithm (Al Rwahnih et al. 2018). A total of 47,261,138 and 8,812,296 single-end reads were obtained from cDNA libraries of PC01 and C. quinoa, respectively. The de novo assembly generated near-complete contigs resembling AVCaV genome ) from both PC01 and C. quinoa, which were 99.8% identical at the nucleotide level. The longest contig (8,342 nucleotides, 73.5x coverage depth) obtained from PC01 was further completed using SMARTer RACE 5’/3’ kit (Takara Bio). The complete genome sequence of AVCaV-PC01 is 8,364 nucleotides long (GenBank: MK170158). The full-length virus genome was compared with GenBank database using BLASTn, which the best hit corresponded to KY132099 with 98% identity. Additionally, AVCaV infection was confirmed in both PC01 selection and the symptomatic C. quinoa by RT-PCR as previously described (Marais et al. 2015). Lastly, symptomatic leaves of C. quinoa were used in leaf dip method to visualize virus particles by transmission electron microscope. As a result, flexuous rod-shaped virions were observed from leaf dips of symptomatic C. quinoa plants (Figure S2). Therefore, our results represent the first report of AVCaV in California, USA. Furthermore, mechanical transmission of an AVCaV isolate infecting flowering apricot to herbaceous hosts was confirmed. Field surveys and biological studies are underway to determine the prevalence of AVCaV in commercial orchards and assess its effect on tree performance.

scholarly journals First Report of Catharanthus mosaic virus in Mandevilla in the United States

Plant Disease ◽  
2015 ◽  
Vol 99 (1) ◽  
pp. 165-165 ◽  
Author(s):  
D. Mollov ◽  
M. A. Guaragna ◽  
B. Lockhart ◽  
J. A. M. Rezende ◽  
R. Jordan
Keyword(s):  
United States ◽  
Amino Acid ◽  
Mosaic Virus ◽  
Consensus Sequence ◽  
The United States ◽  
Amino Acid Sequences ◽  
Universal Primers ◽  
Rt Pcr ◽  
First Report ◽  
Virus Particles

Mandevilla (Apocynaceae) is an ornamental tropical vine popular for its bright and attractive flowers. During 2012 to 2013, 12 Mandevilla sp. samples from Minnesota and Florida nurseries were submitted for analysis at the University of Minnesota Plant Disease Clinic. Plants showed mosaic symptoms, leaf deformation, premature leaf senescence, and vine dieback. Filamentous virus particles with modal lengths 700 to 900 nm were observed by transmission electron microscopy (TEM) in partially purified preparations from symptomatic leaves. Partially purified virions were obtained using 30% sucrose cushion centrifuged at 109,000 gmax for 2 h at 10°C (5). No other virus particles were observed in these samples, nor were any observed in non-symptomatic samples. One sample was submitted as potted plant (Mandevilla ‘Sunmandeho’ Sun Parasol Giant White) and was kept under greenhouse conditions for subsequent analyses. Total RNA (Qiagen) was extracted from this sample, and Potyvirus was detected using the universal primers Poty S (5′-GGN AAY AAY AGY GGN CAR CC-3′) and PV1 (5′-20(T)V-3′) (1) by reverse transcription (RT)-PCR (3). The amplified product was the expected ~1.7-kb, corresponding to the partial nuclear inclusion body gene, the coat protein (CP) gene, and the 3′ end untranslated region. The RT-PCR amplicon was cloned (NEB) and sequenced, and the 1,720-bp consensus sequence was deposited in GenBank (Accession No. KM243928). NCBI BLAST analysis at the nucleotide level revealed highest identity (83%) with an isolate of Catharanthus mosaic virus (CatMV) from Brazil (Accession No. DQ365928). Pairwise analysis of the predicted 256 amino acid CP revealed 91% identity with the CatMV Brazilian isolate (ABI94824) and 68% or less identity with other potyviruses. Two potyviruses are usually considered the same species if their CP amino acid sequences are greater than 80% identical (2). Serological analysis of the infected sample Mandevilla ‘Sunmandeho’ Sun Parasol Giant White using a CatMV specific antiserum (4) resulted in positive indirect ELISA reactions. CatMV has been previously reported in periwinkle (Catharanthus roseus) in Brazil (4). Based on the analyses by TEM, RT-PCR, nucleotide and amino acid sequence identities, and serological reactivity, we identify this virus as a U.S. Mandevilla isolate of CatMV. To our knowledge, this is the first report of Catharanthus mosaic virus both in the United States and in Mandevilla. References: (1) J. Chen et al. Arch Virol. 146:757, 2001. (2) A. Gibbs and K. Ohshima. Ann. Rev. Phytopathol. 48:205, 2010. (3) R. L. Jordan et al. Acta Hortic. 901:159, 2011. (4) S. C. Maciell et al. Sci. Agric. Piracicaba, Brazil. 68:687, 2011. (5) D. Mollov et al. Arch Virol. 158:1917, 2013.

scholarly journals First Report of Tomato infectious chlorosis virus in Spain

Plant Disease ◽  
2002 ◽  
Vol 86 (6) ◽  
pp. 696-696 ◽  
Author(s):  
M. I. Font ◽  
P. Martínez-Culebras ◽  
M. C. Jorda ◽  
D. Louro ◽  
A. M. Vaira ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
The United States ◽  
Mixed Infections ◽  
Chain Reaction ◽  
First Report ◽  
Dna Fragment ◽  
Polymerase Chain ◽  
Plant Pathol ◽  
Tomato Chlorosis Virus ◽  
Tomato Infectious Chlorosis Virus

During the summer and autumn of 2001, symptoms of interveinal yellowing, bronzing, brittleness, and rolling of lower leaves were observed in greenhouse- and field-grown tomato (Lycopersicon esculentum) plants in Castellon Province in eastern Spain. Symptoms resembled those caused by the whitefly-transmitted criniviruses (1,2). Total RNA was extracted from 28 samples of symptomatic leaves collected in three greenhouses and one field and analyzed by reverse transcription-polymerase chain reaction using primers specific for Tomato chlorosis virus (ToCV) (1) and Tomato infectious chlorosis virus (TICV) (2). The 501-bp TICV-specific DNA fragment was amplified in four samples collected during the summer in three greenhouses and one field, and the 439-bp ToCV-specific DNA fragment was amplified in 15 samples collected during the autumn in the same three greenhouses; no mixed infections were found. The DNA fragments amplified from TICV were sequenced and showed 99 to 100% identity with the TICV isolates (GenBank Accession Nos. U67449 and AY048855) from the United States and Italy, respectively, confirming the diagnosis. One sequence was deposited as GenBank Accession No. AF479662. To our knowledge, this is the first report of TICV in Spain and the second in Europe. References: (1) D. Louro et al. Eur. J. Plant Pathol. 106:539, 2000. (2) A. M. Vaira et al. Phytoparasitica. In Press.

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