scholarly journals First Report of the East-Asian Type of Bursaphelenchus mucronatus in Pinus sylvestris in Slovenia

Plant Disease ◽  
2005 ◽  
Vol 89 (9) ◽  
pp. 1015-1015
Author(s):  
G. Urek ◽  
S. Širca
Keyword(s):  
Pinus Sylvestris ◽  
East Asian ◽  
Abies Alba ◽  
Rflp Analysis ◽  
The European Union ◽  
Conifer Forest ◽  
Bursaphelenchus Mucronatus ◽  
Three Samples ◽  
Pine Trees ◽  
First Time

Bursaphelenchus mucronatus Mamiya & Enda, a nematode associated with aboveground tissues of pine trees, is very similar morphologically to the pine wood nematode (PWN), B. xylophilus, a devastating pest that has caused widespread losses to pine woods in some parts of Asia since the early 1900s. During 1999, B. xylophilus was identified for the first time in Europe in Portugal (3), leading to concern that pines and other conifers all over Europe might be endangered. To protect forests from PWN and other pests, the European Union and other countries now regulate the importation of all coniferous chips, sawn wood, and logs and also have organized surveys to determine the extend of PWN infestation. Slovenia has approximately 1,227,832 ha of forest that makes up more than 60% of the surface of the country. The percentage of conifers within forests is 47.9% and comprised mainly of Picea, Pinus, Abies, and Larix species (2). During the summers of 2002-2004, approximately 100 ha of conifer forests in Slovenia were surveyed for the presence of PWN. In total, 170 wood samples from dead and dying pine (Pinus sylvestris L., P. nigra Arnold), spruce (Picea abies L.), and fir (Abies alba Miller) were analyzed. B. xylophilus (Steiner & Buhrer) Nickle was not found in the area, but a closely related nematode, B. mucronatus, previously shown to be pathogenic on conifer seedlings (4), was isolated from samples of P. sylvestris. Of 34 P. sylvestris samples analyzed, B. mucronatus was found in three samples. Samples were taken from a conifer forest located in the central part of Slovenia near the international airport of Ljubljana. The species was identified morphologically and also characterized by polymerase chain reaction restriction (PCR)-fragment length polymorphism (RFLP) analysis on the basis of ITS-rDNA fragment patterns. According to RFLP patterns (1), the analyzed species was recognized as the East-Asian type of B. mucronatus. Beside these findings, the specimens of B. homanni (Braasch) and Bursaphelenchus sp. have also been found in Slovenia. The results of the survey show that PWN was not found in Slovenia, but three different species of the Bursaphelenchus genus were present. References: (1) H. Braasch et al. Nachrbl. Dtsch. Pflanzenschutzd. 51:312, 1999. (2) M. Jurc et al. Zb. Gozdarstva in Lesarstva 72:121, 2004. (3) M. M. Mota et al. Nematology 1:727, 1999. (4) J. R. Sutherland. Pine wilt nematode. Pages 19–20 in: Compendium of Conifer Diseases. E. M. Hansen and K. J. Lewis, eds. The American Phytopathological Society, St. Paul, MN. 1997.

scholarly journals Bursaphelenchus Fuchs, 1937 (Nematoda: Parasitaphelenchidae) species associated with Pinus species in northern Turkey

2008 ◽  
Vol 45 (2) ◽  
pp. 89-95 ◽  
Author(s):  
S. Akbulut ◽  
P. Vieira ◽  
A. Ryss ◽  
V. Valadas ◽  
A. Keten ◽  
...  
Keyword(s):  
Ribosomal Dna ◽  
Rflp Analysis ◽  
Pinus Nigra ◽  
Black Pine ◽  
First Report ◽  
Its Regions ◽  
Northern Regions ◽  
Pine Trees ◽  
Conifer Trees ◽  
First Time

AbstractA survey for Bursaphelenchus nematodes, associated with different conifer trees, was conducted in several forest areas in the northern regions of Turkey. Only pine trees (Pinus nigra, P. pinaster and P. sylvestris) yielded Bursaphelenchus specimens. Nematodes were identified using several morphological diagnostic characters of the genus (male spicule structure, number of lateral incisures, number and distribution of the male papillae, presence of female vulval flap), and confirmed by using RFLP analysis of the internal transcriber spacer (ITS) regions of ribosomal DNA. Three different species were identified from several sampled areas, namely B. mucronatus, B. pinophilus and B. sexdentati, representing a first report of the last two species for Turkey. The association of B. pinophilus with black pine (P. nigra) is herein reported for the first time.

Application of Novel Low Current OBIRCH Amplifier and Nanoprobing to Identify Subtle Leakages in Advanced Node Technologies

2018 ◽  
Author(s):  
Satish Kodali ◽  
Liangshan Chen ◽  
Yuting Wei ◽  
Tanya Schaeffer ◽  
Chong Khiam Oh
Keyword(s):  
Signal To Noise Ratio ◽  
Semiconductor Industry ◽  
Fault Isolation ◽  
Ring Oscillator ◽  
High Signal ◽  
The Novel ◽  
Resistance Change ◽  
Three Samples ◽  
Relative Threshold ◽  
First Time

Abstract Optical beam induced resistance change (OBIRCH) is a very well-adapted technique for static fault isolation in the semiconductor industry. Novel low current OBIRCH amplifier is used to facilitate safe test condition requirements for advanced nodes. This paper shows the differences between the earlier and novel generation OBIRCH amplifiers. Ring oscillator high standby leakage samples are analyzed using the novel generation amplifier. High signal to noise ratio at applied low bias and current levels on device under test are shown on various samples. Further, a metric to demonstrate the SNR to device performance is also discussed. OBIRCH analysis is performed on all the three samples for nanoprobing of, and physical characterization on, the leakage. The resulting spots were calibrated and classified. It is noted that the calibration metric can be successfully used for the first time to estimate the relative threshold voltage of individual transistors in advanced process nodes.

Comparative Study of a and b- pinene Content in Volatile Oils of Abies alba, Pinus sylvestris, Juniperus communis, Rosmarinus officinalis, Salvia officinalis and Coriandrum sativum

2018 ◽  
Vol 69 (9) ◽  
pp. 2338-2342
Author(s):  
Mariana Popescu ◽  
Diana Puiu ◽  
Anca Daniela Raiciu
Keyword(s):  
Pinus Sylvestris ◽  
Raw Materials ◽  
Abies Alba ◽  
Volatile Oil ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Coriandrum Sativum ◽  
Volatile Oils ◽  
Juniperus Communis ◽  
Chromatographic Profile

The study describes the composition of volatile oils obtained by steam distillation of water fir (Abies alba), pine (Pinus sylvestris), juniper (Juniperus communis), rosemary (Rosmarinus officinalis), sage (Salvia officinalis), and coriander (Coriandrum sativum) using the gas chromatography technique coupled with mass spectrometry (GC-MS). Chromatographic profile revealed the presence of specific components for each studied volatile oil. It noted the presence of a and b- pinene in all volatile oils studied at different concentrations. Volatile oils studied, from green sources and rich in a, b-pinene will be used as raw materials for creating products with anti-inflammatory, antiseptic, bronchodilators properties.

Feasibility and Evaluation of Automated Methods for Radiolabeling of Radiopharmaceutical Kits with Gallium-68

2019 ◽  
Vol 12 (3) ◽  
pp. 229-237 ◽  
Author(s):  
Alban Revy ◽  
François Hallouard ◽  
Sandrine Joyeux-Klamber ◽  
Andrea Skanjeti ◽  
Catherine Rioufol ◽  
...  
Keyword(s):  
The European Union ◽  
Preparation Time ◽  
Limit Of Quantification ◽  
Manual Method ◽  
Personnel Costs ◽  
Automated Method ◽  
Gallium 68 ◽  
The Right ◽  
First Time ◽  
Automated Methods

Objective: Recent gallium-68 labeled peptides are of increasing interest in PET imaging in nuclear medicine. Somakit TOC® is a radiopharmaceutical kit registered in the European Union for the preparation of [68Ga]Ga-DOTA-TOC used for the diagnosis of neuroendocrine tumors. Development of a labeling process using a synthesizer is particularly interesting for the quality and reproducibility of the final product although only manual processes are described in the Summary of Product (SmPC) of the registered product. The aim of the present study was therefore to evaluate the feasibility and value of using an automated synthesizer for the preparation of [68Ga]Ga-DOTA-TOC according to the SmPC of the Somakit TOC®. Methods: Three methods of preparation were compared; each followed the SmPC of the Somakit TOC®. Over time, overheads, and overexposure were evaluated for each method. Results: Mean±SD preparation time was 26.2±0.3 minutes for the manual method, 28±0.5 minutes for the semi-automated, and 40.3±0.2 minutes for the automated method. Overcost of the semi-automated method is 0.25€ per preparation for consumables and from 0.58€ to 0.92€ for personnel costs according to the operator (respectively, technician or pharmacist). For the automated method, overcost is 70€ for consumables and from 4.06€ to 6.44€ for personnel. For the manual method, extremity exposure was 0.425mSv for the right finger, and 0.350mSv for the left finger; for both the semi-automated and automated method extremity exposure were below the limit of quantification. Conclusion: The present study reports for the first time both the feasibility of using a [68Ga]- radiopharmaceutical kit with a synthesizer and the limits for the development of a fully automated process.

To bee or not to beet with neonicotinoids

2021 ◽  
Vol 23 (2) ◽  
pp. 103-109
Author(s):  
Lynda M. Warren
Keyword(s):  
Sugar Beet ◽  
Weather Conditions ◽  
The European Union ◽  
Environmental Standards ◽  
Economic Interests ◽  
Precautionary Approach ◽  
The United Kingdom ◽  
Commercial Sugar ◽  
The Uk ◽  
First Time

In January 2021 the UK government granted an application for authorisation to use thiamethoxam, a neonicotinoid pesticide, to protect commercial sugar beet crops from attack by viruses transmitted by aphids. This was the first time such an authorisation had been granted in the United Kingdom (UK) and there were concerns that it signalled a weakening of environmental standards now that the UK was no longer part of the European Union. In fact, similar authorisations had been granted by several European Member States in the last 2 years, despite the ban on the use of neonicotinoids introduced in 2018. Nevertheless, the reasons for granting the authorisation do suggest that the balance between adopting a precautionary approach to environmental protection and taking emergency action to protect economic interests may have shifted. It was acknowledged that the proposed mitigation to safeguard bees and other wildlife was not entirely satisfactory. In the end, due to unforeseen weather conditions it meant that the pesticide is not necessary, which in itself demonstrates that short-term emergency measures are unsuitable for dealing with the problem. If the sugar beet industry is to continue to prosper in the UK, it will need to be managed in a way that provides resistance to virus infection without the use of controversial chemicals.

scholarly journals Changes in Nitrogen-Fixing and Ammonia-Oxidizing Bacterial Communities in Soil of a Mixed Conifer Forest after Wildfire

2005 ◽  
Vol 71 (5) ◽  
pp. 2713-2722 ◽  
Author(s):  
Chris M. Yeager ◽  
Diana E. Northup ◽  
Christy C. Grow ◽  
Susan M. Barns ◽  
Cheryl R. Kuske
Keyword(s):  
Bacterial Communities ◽  
Fragment Length Polymorphism ◽  
Rflp Analysis ◽  
Ammonia Oxidizing Bacteria ◽  
Nitrogen Fixing ◽  
Conifer Forest ◽  
Mixed Conifer ◽  
Mixed Conifer Forest ◽  
In Fire ◽  
Sequence Types

ABSTRACT This study was undertaken to examine the effects of forest fire on two important groups of N-cycling bacteria in soil, the nitrogen-fixing and ammonia-oxidizing bacteria. Sequence and terminal restriction fragment length polymorphism (T-RFLP) analysis of nifH and amoA PCR amplicons was performed on DNA samples from unburned, moderately burned, and severely burned soils of a mixed conifer forest. PCR results indicated that the soil biomass and proportion of nitrogen-fixing and ammonia-oxidizing species was less in soil from the fire-impacted sites than from the unburned sites. The number of dominant nifH sequence types was greater in fire-impacted soils, and nifH sequences that were most closely related to those from the spore-forming taxa Clostridium and Paenibacillus were more abundant in the burned soils. In T-RFLP patterns of the ammonia-oxidizing community, terminal restriction fragments (TRFs) representing amoA cluster 1, 2, or 4 Nitrosospira spp. were dominant (80 to 90%) in unburned soils, while TRFs representing amoA cluster 3A Nitrosospira spp. dominated (65 to 95%) in fire-impacted soils. The dominance of amoA cluster 3A Nitrosospira spp. sequence types was positively correlated with soil pH (5.6 to 7.5) and NH3-N levels (0.002 to 0.976 ppm), both of which were higher in burned soils. The decreased microbial biomass and shift in nitrogen-fixing and ammonia-oxidizing communities were still evident in fire-impacted soils collected 14 months after the fire.

scholarly journals Scots pine (Pinus sylvestris) dynamics in the Welsh Marches during the mid to late-Holocene

The Holocene ◽  
2021 ◽  
pp. 095968362199465
Author(s):  
Dael Sassoon ◽  
William J Fletcher ◽  
Alastair Hotchkiss ◽  
Fern Owen ◽  
Liting Feng
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
Late Holocene ◽  
British Isles ◽  
Loss On Ignition ◽  
Peat Core ◽  
New Information ◽  
Pine Trees ◽  
Pollen Records

Around 4000 cal yr BP, Scots pine ( Pinus sylvestris) suffered a widespread demise across the British Isles. This paper presents new information about P. sylvestris populations found in the Welsh Marches (western central Britain), for which the long-term history and origins are poorly known. Two new pollen records were produced from the Lin Can Moss ombrotrophic bog (LM18) and the Breidden Hill pond (BH18). The LM18 peat core is supported by loss-on-ignition, humification analysis and radiocarbon dating. Lead concentrations were used to provide an estimated timeframe for the recent BH18 record. In contrast to many other Holocene pollen records from the British Isles, analysis of LM18 reveals that Scots pine grains were deposited continuously between c. 6900–300 cal yr BP, at frequencies of 0.3–5.4%. It is possible that individual Scots pine trees persisted through the wider demise on thin soils of steep drought-prone crags of hills or the fringes of lowland bogs in the Welsh Marches. At BH18, the record indicates a transition from broadleaved to mixed woodland, including conifer species introduced around AD 1850 including Picea and Pinus. The insights from BH18 suggest that the current populations may largely be the result of planting. Comparison of the LM18 findings with other regional pollen records highlights consistent patterns, including a Mid-Holocene maximum (ca. 7000 cal yr BP), long-term persistence at low pollen percentages and a Late-Holocene minimum (ca. 3000 cal yr BP). These distinctive trends encourage further studies on refugial areas for Scots pine in this region and elsewhere.

STECCHERINUM AURANTILAETUM (CORNER) BERNICCHIA ET GORJÓN (BASIDIOMYCOTA) IN THE FAR EAST OF RUSSIA

2021 ◽  
Vol 69 ◽  
Author(s):  
N.V. Bukharova ◽  
Keyword(s):  
Nature Reserve ◽  
East Asian ◽  
Far East ◽  
Original Description ◽  
General Distribution ◽  
Far East Of Russia ◽  
Autonomous Region ◽  
The Far East ◽  
Far Eastern ◽  
First Time

Steccherinum aurantilaetum is a predominantly East Asian polyporoid fungus from the Steccherinaceae. It was first discovered in the Krasnoarmeisky District of the Primorye and in the Khabarovsk Territory. Previously, it was known only in the «Kedrovaya Pad» Nature Reserve in the Primorye and in the «Bastak» Nature Reserve in the Jewish Autonomous Region (for the territory of Russia). An original description of the species based on Far Eastern material is given, and a map of the general distribution of S. aurantilaetum is presented for the first time.

Implementing the London Dumping Convention in East Asia

2017 ◽  
Vol 2 (2) ◽  
pp. 247-267 ◽  
Author(s):  
Keyuan Zou ◽  
Lei Zhang
Keyword(s):  
South Korea ◽  
East Asia ◽  
Carbon Storage ◽  
Marine Pollution ◽  
East Asian ◽  
The Philippines ◽  
New Challenges ◽  
The Republic ◽  
London Protocol ◽  
First Time

In 1972, the Convention on the Prevention of Marine Pollution by Dumping of Wastes and Other Matter (London Convention) was negotiated. It is a global treaty, for the first time, to regulate dumping of waste at sea worldwide. Following this global endeavor, the Protocol to the London Convention (London Protocol) was later agreed to further modernize the London Convention so as to reinforce the management of dumping of waste at sea. While in East Asia, only China, Japan, the Republic of Korea (South Korea) and the Philippines have acceded to the Convention and its Protocol, other countries do not show their willingness to sign them. Against this background, this article will address the responses of these East Asian states to the implementation of the London Convention, and analyze and assess their relevant laws and regulations with particular reference to China’s practice. In addition, it will focus on new challenges, such as offshore carbon storage, to the London Convention.

A revision of the subgenus Leucandrena of the genus Andrena of eastern Asia (Hymenoptera: Apoidea: Andrenidae)

Zootaxa ◽  
2009 ◽  
Vol 2145 (1) ◽  
pp. 36-46
Author(s):  
HUANLI XU ◽  
OSAMU TADAUCHI
Keyword(s):  
New Species ◽  
East Asian ◽  
Eastern Asia ◽  
New Synonyms ◽  
Two New Species ◽  
First Time

The subgenus Leucandrena of the genus Andrena of eastern Asia is revised, and seven species are recognized. Two new species, Andrena (Leucandrena) paramelanospila and Andrena (Leucandrena) banchan are described from Beijing and Xizang (China) respectively, and the male of Andrena (Leucandrena) melanospila Cockerell is described for the first time. A. (Euandrena) taisetsusana Tadauchi et Hirashima, and A. (Andrena) dolharubang Tadauchi and Xu are recognized as new synonyms of the Holarctic A. barbilabris (Kirby). A key to East Asian Leucandrena is given.

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