Pathotypes ofPuccinia hordeiwith Virulence for the Barley Leaf Rust Resistance GeneRph7 in the United States

Plant Disease ◽  
1993 ◽  
Vol 77 (9) ◽  
pp. 867 ◽  
Author(s):  
Brian J. Steffenson
Keyword(s):  
United States ◽  
Leaf Rust ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
The United States ◽  
Barley Leaf Rust ◽  
Barley Leaf

Validating molecular markers for barley leaf rust resistance genes Rph5 and Rph7

2007 ◽  
Vol 126 (5) ◽  
pp. 458-463 ◽  
Author(s):  
J. A. Mammadov ◽  
W. S. Brooks ◽  
C. A. Griffey ◽  
M. A. Saghai Maroof
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Barley Leaf Rust ◽  
Barley Leaf ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

scholarly journals Differentiation of Molecular Genotypes and Virulence Phenotypes of Puccinia triticina from Common Wheat in North America

2009 ◽  
Vol 99 (6) ◽  
pp. 750-758 ◽  
Author(s):  
M. E. Ordoñez ◽  
J. A. Kolmer
Keyword(s):  
United States ◽  
North America ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
The United States ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Wheat leaf rust caused by Puccinia triticina is widely distributed in the wheat growing regions of the United States and Canada, and is subject to selection for virulence phenotype by leaf rust resistance genes in wheat cultivars. The objective of this study was to determine the number of genetically differentiated groups of P. triticina that are currently present in North America. In total, 148 isolates of P. triticina from the 1980s to 2005 were collected from wheat-growing regions of the United States and Canada and tested for virulence on 20 lines of wheat with single genes for leaf rust resistance and for molecular genotype with 23 simple sequence repeat (SSR) markers. In total, 91 virulence phenotypes and 65 SSR genotypes were found. After removal of isolates with identical virulence and SSR genotypes, 125 isolates were included for further analysis. Bayesian cluster analysis indicated five different groups of isolates based on SSR genotypes that also differed for virulence to leaf rust resistance genes Lr2a, Lr2c, Lr3bg, Lr17, and Lr28. Isolates avirulent to Lr14a and Lr20 that have increased since 2003 had SSR genotypes identical or similar to older isolates in one of the five groups, indicating that these isolates were derived by mutation from the previously existing population of P. triticina. The representative collection of P. triticina isolates had characteristics consistent with an asexual dikaryotic population of genetically differentiated groups of SSR genotypes with high levels of heterozygosity and disequilibrium within which stepwise mutation at avirulence or virulence loci regularly occurs.

scholarly journals First Detection of Puccinia hordei Virulence to Barley Leaf Rust Resistance Gene Rph3 and Combination with Virulence to Rph7 in North America

Plant Disease ◽  
2013 ◽  
Vol 97 (6) ◽  
pp. 838-838 ◽  
Author(s):  
M. N. Rouse ◽  
C. A. Griffey ◽  
W. S. Brooks
Keyword(s):  
North America ◽  
Leaf Rust ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Puccinia Hordei ◽  
Barley Leaf Rust ◽  
Barley Leaf ◽  
Infection Types ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

Barley leaf rust, caused by Puccinia hordei Otth., has been problematic in United States barley (Hordeum vulgare L.) production in the Mid-Atlantic coast region and California. During the early 1990s, P. hordei pathotypes with virulence to resistance gene Rph7 caused average yield losses from 6 to 16% (3). ‘Doyce’ barley was released in 2003 and was described as being resistant to leaf rust (2). Initially in April 2010 and subsequently in spring 2011 and 2012, high severities and infection responses were observed on experimental plots of ‘Doyce’ in Warsaw and Blacksburg, Virginia. Three single uredinial isolates of P. hordei were derived from collections made from ‘Doyce’ barley. The isolates were characterized for virulence to barley leaf rust resistance genes by inoculating at least two replicates of a barley leaf rust differential set including 12 Rph genes (1). Previous methods used for inoculation, incubation, and pathotyping were followed (1). Infection types were scored on a 0 to 4 scale where 2 and below indicated resistance and 3 and above indicated susceptibility (4). The three isolates collected from Doyce barley displayed large pustules with infection types 3,3+ to cultivars Estate (Rph3) and Cebada Capa (Rph7). Avirulent isolates of P. hordei displayed infection types 0; to 0;1c to Estate and ;n to 0;1n to Cebada Capa (1). The data indicated that all three isolates were virulent to both barley leaf rust resistance genes Rph3 and Rph7. Though combined Rph3 and Rph7 virulence has been reported in the Mediterranean region, this is the first report of Rph3 virulence in North America. These isolates of P. hordei are virulent to important sources of resistance to barley leaf rust and threaten barley production in environments conducive for disease development in North America. References: (1) W. S. Brooks et al. Phytopathology 90:1131, 2000. (2) W. S. Brooks et al. Crop Sci. 45:792, 2005. (3) C. A. Griffey et al. Plant Dis. 78:256, 1994. (4) M. N. Levine and W. J. Cherewick. U.S. Dept. Agric. Tech. Bull. 1056, 1952.

Genetic mapping of a barley leaf rust resistance gene Rph26 introgressed from Hordeum bulbosum

2018 ◽  
Vol 131 (12) ◽  
pp. 2567-2580 ◽  
Author(s):  
Xiaohui Yu ◽  
Hoi Yee Kong ◽  
Vijitha Meiyalaghan ◽  
Seona Casonato ◽  
Soonie Chng ◽  
...  
Keyword(s):  
Genetic Mapping ◽  
Leaf Rust ◽  
Resistance Gene ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Rust Resistance Gene ◽  
Leaf Rust Resistance Gene ◽  
Hordeum Bulbosum ◽  
Barley Leaf Rust ◽  
Barley Leaf

scholarly journals Physiologic Specialization of Puccinia triticina on Wheat in the United States in 2005

Plant Disease ◽  
2007 ◽  
Vol 91 (8) ◽  
pp. 979-984 ◽  
Author(s):  
J. A. Kolmer ◽  
D. L. Long ◽  
M. E. Hughes
Keyword(s):  
United States ◽  
Leaf Rust ◽  
Great Plains ◽  
Durable Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
The United States ◽  
Ohio River ◽  
Virulence Phenotype ◽  
Common Phenotype

Collections of Puccinia triticina were obtained from rust-infected wheat leaves by cooperators throughout the United States and from surveys of wheat fields and nurseries in the Great Plains, Ohio River Valley, southeast, California, and Washington State, in order to determine the virulence of the wheat leaf rust population in 2005. Single uredinial isolates (797 in total) were derived from the collections and tested for virulence phenotype on lines of Thatcher wheat that are near-isogenic for leaf rust resistance genes Lr1, Lr2a, Lr2c, Lr3a, Lr9, Lr16, Lr24, Lr26, Lr3ka, Lr11, Lr17a, Lr30, LrB, Lr10, Lr14a, Lr18, Lr21, Lr28, and winter wheat lines with genes Lr41 and Lr42. In the United States in 2005, 72 virulence phenotypes of P. triticina were found. Virulence phenotype TDBGH, selected by virulence to resistance gene Lr24, was the most common phenotype in the United States, and was found throughout the Great Plains region. Virulence phenotype MCDSB with virulence to Lr17a and Lr26 was the second most common phenotype and was found widely in the wheat growing regions of the United States. Virulence phenotype MFPSC, which has virulence to Lr17a, Lr24, and Lr26, was the third most common phenotype, and was found in the Ohio Valley region, the Great Plains, and California. The highly diverse population of P. triticina in the United States will continue to present a challenge for the development of wheat cultivars with effective durable resistance to leaf rust.

scholarly journals Physiologic Specialization of Puccinia triticina on Wheat in the United States in 2007

Plant Disease ◽  
2009 ◽  
Vol 93 (5) ◽  
pp. 538-544 ◽  
Author(s):  
J. A. Kolmer ◽  
D. L. Long ◽  
M. E. Hughes
Keyword(s):  
United States ◽  
Winter Wheat ◽  
Leaf Rust ◽  
Great Plains ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
The United States ◽  
Northern Great Plains ◽  
Ohio River ◽  
Soft Red Winter Wheat

In 2007, leaf rust of wheat was severe throughout the Great Plains region of North America. Yield losses in wheat due to leaf rust were estimated to be 14% in Kansas. Collections of Puccinia triticina were obtained from rust-infected leaves provided by cooperators throughout the United States and from surveys of wheat fields and nurseries in the Great Plains, Ohio River Valley, southeast, California, and Washington State in order to determine the virulence of the wheat leaf rust population in 2007. Single uredinial isolates (868 in total) were derived from the collections and tested for virulence phenotype on lines of Thatcher wheat that are near-isogenic for leaf rust resistance genes Lr1, Lr2a, Lr2c, Lr3a, Lr9, Lr16, Lr24, Lr26, Lr3ka, Lr11, Lr17a, Lr30, LrB, Lr10, Lr14a, Lr18, Lr21, and Lr28, and on winter wheat lines with genes Lr41 and Lr42. Fifty-two virulence phenotypes were found. Virulence phenotypes TDBJG, MFPSC, and TDBJH were among the four most common phenotypes and were all virulent to resistance gene Lr24. These phenotypes were found throughout the Great Plains region. Phenotype MLDSD, with virulence to Lr9, Lr17, and Lr41, was also widely distributed in the Great Plains. In the soft red winter wheat region of the southeastern states, phenotypes TCRKG, with virulence to genes Lr11, Lr26, and Lr18, and MFGJH, with virulence to Lr24, Lr26, and Lr11, were among the common phenotypes. Virulence phenotypes with virulence to Lr16 were most frequent in the spring wheat region of the northern Great Plains. Virulence phenotypes with virulence to Lr11, Lr18, and Lr26 were most common in the soft red winter areas of the southeastern states and Ohio Valley. Virulence to Lr21 was not found in any of the tested isolates.

scholarly journals Physiologic Specialization of Puccinia triticina on Wheat in the United States in 2004

Plant Disease ◽  
2006 ◽  
Vol 90 (9) ◽  
pp. 1219-1224 ◽  
Author(s):  
J. A. Kolmer ◽  
D. L. Long ◽  
M. E. Hughes
Keyword(s):  
United States ◽  
Leaf Rust ◽  
Great Plains ◽  
Resistance Genes ◽  
Durable Resistance ◽  
Puccinia Triticina ◽  
Leaf Rust Resistance ◽  
The United States ◽  
Virulence Phenotype ◽  
Common Phenotype

Collections of Puccinia triticina were obtained from rust-infected wheat leaves by cooperators throughout the United States and from surveys of wheat fields and nurseries in the Great Plains, Ohio Valley, southeast, California, and Pacific Northwest, in order to determine the virulence of the wheat leaf rust population in 2004. Single uredinial isolates (757 in total) were derived from the collections and tested for virulence phenotype on lines of Thatcher wheat that are near-isogenic for leaf rust resistance genes Lr1, Lr2a, Lr2c, Lr3a, Lr9, Lr16, Lr24, Lr26, Lr3ka, Lr11, Lr17a, Lr30, LrB, Lr10, Lr14a, Lr18, Lr21, and Lr28, and winter wheat lines with genes Lr41 and Lr42. In the United States in 2004, 52 virulence phenotypes of P. triticina were found. Virulence phenotype MCDSB, selected by virulence to resistance genes Lr17a and Lr26, was the most common phenotype in the United States and was found in all wheat growing areas. Virulence phenotype TBBGG, with virulence to Lr2a, was the second most common phenotype and was found primarily in the spring wheat region of the north-central states. Virulence phenotype MBDSB, which has virulence to Lr17a, was the third most common phenotype and was found in all wheat growing areas except California. Phenotype TNRJJ, with virulence to genes Lr9, Lr24, and Lr41, was the fourth most common phenotype and occurred in the southeastern states and throughout the Great Plains region. Virulence phenotypes avirulent to a second gene in the Thatcher differential line with Lr1 increased in frequency in the United States in 2004. The highly diverse population of P. triticina in the United States will continue to present a challenge for the development of wheat cultivars with effective durable resistance.

scholarly journals Validating molecular markers for barley leaf rust resistance genes Rph20 and Rph24

2020 ◽  
Author(s):  
PM Dracatos ◽  
RF Park ◽  
D Singh
Keyword(s):  
Molecular Markers ◽  
Leaf Rust ◽  
Resistance Genes ◽  
Leaf Rust Resistance ◽  
Puccinia Hordei ◽  
Adult Plant ◽  
Barley Leaf Rust ◽  
Efficient Gene ◽  
Barley Leaf ◽  
Barley Germplasm

Improving resistance to barley leaf rust (caused by Puccinia hordei) is an important breeding objective in most barley growing regions worldwide. The development and subsequent utilisation of high-throughput PCR-based co-dominant molecular markers remains an effective approach to select genotypes with multiple effective resistance genes, permitting efficient gene deployment and stewardship. The genes Rph20 and Rph24 confer widely effective adult plant resistance (APR) to leaf rust, are common in European and Australian barley germplasm (often in combination), and act interactively to confer high levels of resistance (Dracatos et al. 2015; Zeims et al. 2017; Singh et al. 2018). Here we report on the development and validation of co-dominant insertion-deletion (indel) based PCR markers that are highly predictive for the Rph20 and Rph24 resistances.

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