scholarly journals An HR-Induced Tobacco Peroxidase Gene Is Responsive to Spermine, but Not to Salicylate, Methyl Jasmonate, and Ethephon

2000 ◽  
Vol 13 (2) ◽  
pp. 210-216 ◽  
Author(s):  
Susumu Hiraga ◽  
Hiroyuki Ito ◽  
Hiromoto Yamakawa ◽  
Norihiro Ohtsubo ◽  
Shigemi Seo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Methyl Jasmonate ◽  
Hypersensitive Reaction ◽  
N Gene ◽  
Pr Genes ◽  
Tobacco Plants ◽  
Induced Expression ◽  
Peroxidase Gene ◽  
Induction Kinetics ◽  
Tobacco Peroxidase

In Tobacco mosaic virus (TMV)-infected tobacco plants carrying the N resistance gene, a hypersensitive reaction or response (HR) occurs to enclose the virus in the infected tissue. Although a contribution of peroxidases to the resistance has been proposed, no evidence has been presented that tobacco peroxidase genes respond to HR. Here, we describe the HR-induced expression of a tobacco peroxidase gene (tpoxC1) whose induction kinetics were slightly different from those of acidic and basic tobacco pathogenesis-related (PR) protein genes. Interestingly, tpoxC1 was insensitive to the inducers of PR genes such as salicylic acid, methyl jasmonate, and ethephon. Spermine activated tpoxC1 gene expression at a low level and both acidic and basic PR gene expression at a considerably higher level. These results indicate that the induced expression of tpoxC1 is regulated differently from that of classical tobacco PR genes in the N gene-mediated self-defense system in tobacco plants.

scholarly journals Wound-Induced Expression of a Tobacco Peroxidase is Not Enhanced by Ethephon and Suppressed by Methyl Jasmonate and Coronatine

2000 ◽  
Vol 41 (2) ◽  
pp. 165-170 ◽  
Author(s):  
S. Hiraga ◽  
H. Ito ◽  
K. Sasaki ◽  
H. Yamakawa ◽  
I. Mitsuhara ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Induced Expression ◽  
Tobacco Peroxidase

scholarly journals Interleukin-1β Induced Matrix Metalloproteinase Expression in Human Periodontal Ligament-Derived Mesenchymal Stromal Cells under In Vitro Simulated Static Orthodontic Forces

2021 ◽  
Vol 22 (3) ◽  
pp. 1027
Author(s):  
Christian Behm ◽  
Michael Nemec ◽  
Alice Blufstein ◽  
Maria Schubert ◽  
Xiaohui Rausch-Fan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Periodontal Ligament ◽  
Induced Expression ◽  
Gene And Protein Expression ◽  
Tensile Strains ◽  
Orthodontic Forces ◽  
Low Magnitude

The periodontal ligament (PDL) responds to applied orthodontic forces by extracellular matrix (ECM) remodeling, in which human periodontal ligament-derived mesenchymal stromal cells (hPDL-MSCs) are largely involved by producing matrix metalloproteinases (MMPs) and their local inhibitors (TIMPs). Apart from orthodontic forces, the synthesis of MMPs and TIMPs is influenced by the aseptic inflammation occurring during orthodontic treatment. Interleukin (IL)-1β is one of the most abundant inflammatory mediators in this process and crucially affects the expression of MMPs and TIMPs in the presence of cyclic low-magnitude orthodontic tensile forces. In this study we aimed to investigate, for the first time, how IL-1β induced expression of MMPs, TIMPs and how IL-1β in hPDL-MSCs was changed after applying in vitro low-magnitude orthodontic tensile strains in a static application mode. Hence, primary hPDL-MSCs were stimulated with IL-1β in combination with static tensile strains (STS) with 6% elongation. After 6- and 24 h, MMP-1, MMP-2, TIMP-1 and IL-1β expression levels were measured. STS alone had no influence on the basal expression of investigated target genes, whereas IL-1β caused increased expression of these genes. In combination, they increased the gene and protein expression of MMP-1 and the gene expression of MMP-2 after 24 h. After 6 h, STS reduced IL-1β-induced MMP-1 synthesis and MMP-2 gene expression. IL-1β-induced TIMP-1 gene expression was decreased by STS after 6- and 24-h. At both time points, the IL-1β-induced gene expression of IL-1β was increased. Additionally, this study showed that fetal bovine serum (FBS) caused an overall suppression of IL-1β-induced expression of MMP-1, MMP-2 and TIMP-1. Further, it caused lower or opposite effects of STS on IL-1β-induced expression. These observations suggest that low-magnitude orthodontic tensile strains may favor a more inflammatory and destructive response of hPDL-MSCs when using a static application form and that this response is highly influenced by the presence of FBS in vitro.

scholarly journals Lithium dramatically potentiates neurotensin/neuromedin N gene expression.

1988 ◽  
Vol 263 (28) ◽  
pp. 13983-13986
Author(s):  
P R Dobner ◽  
A S Tischler ◽  
Y C Lee ◽  
S R Bloom ◽  
S R Donahue
Keyword(s):  
Gene Expression ◽  
N Gene ◽  
Neuromedin N

scholarly journals Heat-induced expression of albumin during early stages of rat embryo development.

1987 ◽  
Vol 7 (12) ◽  
pp. 4599-4602 ◽  
Author(s):  
U K Srinivas ◽  
C J Revathi ◽  
M R Das
Keyword(s):  
Gene Expression ◽  
Heat Shock ◽  
Embryonic Liver ◽  
Rat Embryo ◽  
Adult Liver ◽  
Stages Of Development ◽  
Induced Expression ◽  
Molecular Events ◽  
Internal Change ◽  
Shock Proteins

An examination of heat-induced expression of proteins in tissues from adult and embryonic liver in rats shows that albumin, which is constitutively expressed in adult liver and is not synthesized in embryos before 16 days of gestation, appears in liver cells at earlier stages of development upon heat shock. On the basis of available evidence for the expression of heat shock proteins at distinct stages of development and on the basis of our findings, it may be argued that there could be common molecular events taking place during development and as a result of heat shock. We suggest also that one of the consequences of heat shock could be an internal change of pH within the cell which, in turn, might trigger alterations in gene expression.

scholarly journals Lignocellulose Affects Mn2+ Regulation of Peroxidase Transcript Levels in Solid-State Cultures of Pleurotus ostreatus

2002 ◽  
Vol 68 (6) ◽  
pp. 3156-3158 ◽  
Author(s):  
Roni Cohen ◽  
Oded Yarden ◽  
Yitzhak Hadar
Keyword(s):  
Gene Expression ◽  
Solid State ◽  
Direct Effect ◽  
Pleurotus Ostreatus ◽  
Metal Ion ◽  
Transcript Levels ◽  
Peroxidase Gene ◽  
Defined Media ◽  
Cotton Stalks ◽  
Encoding Genes

ABSTRACT The effect of Mn2+ amendment on peroxidase gene expression was studied during Pleurotus ostreatus growth on cotton stalks. Four peroxidase-encoding genes were expressed differentially and in a manner different from that observed in defined media. Mn2+ affects mnp3 expression even 2 h after its addition to the cultures, suggesting a direct effect of the metal ion on expression.

The effects of methyl jasmonate on growth, gene expression and metabolite accumulation in Isatis indigotica Fort.

2022 ◽  
Vol 177 ◽  
pp. 114482
Author(s):  
Rui Liu ◽  
Zirong Wang ◽  
Jinyu Zheng ◽  
Zhaojin Xu ◽  
Xue Tang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Methyl Jasmonate ◽  
Isatis Indigotica ◽  
Metabolite Accumulation
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