Resistance in Transgenic Tobacco Plants Expressing a Nonstructural Gene Sequence of Tobacco Mosaic Virus Is a Consequence of Markedly Reduced Virus Replication

1991 ◽  
Vol 4 (6) ◽  
pp. 579 ◽  
Author(s):  
John P. Carr
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Transgenic Tobacco ◽  
Virus Replication ◽  
Gene Sequence ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants

scholarly journals Replication of Coatless Mutant of Tobacco Mosaic Virus in Transgenic Tobacco Plants.

1993 ◽  
Vol 59 (2) ◽  
pp. 171-174
Author(s):  
Toshiya OGAWA ◽  
Masaharu YOSHIOKA ◽  
Jun YAMAYA ◽  
Yoshimi OKADA
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants

Secondary Plasmodesmata Are Specific Sites of Localization of the Tobacco Mosaic Virus Movement Protein in Transgenic Tobacco Plants

1992 ◽  
Vol 4 (8) ◽  
pp. 915 ◽  
Author(s):  
Biao Ding ◽  
James S. Haudenshield ◽  
Richard J. Hull ◽  
Shmuel Wolf ◽  
Roger N. Beachy ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Transgenic Tobacco ◽  
Movement Protein ◽  
Virus Movement ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Secondary Plasmodesmata

Disease response to tobacco mosaic virus in transgenic tobacco plants that constitutively express the pathogenesis-related PR1b gene

Virology ◽  
1989 ◽  
Vol 173 (1) ◽  
pp. 89-97 ◽  
Author(s):  
John R. Cutt ◽  
Mark H. Harpster ◽  
David C. Dixon ◽  
John P. Carr ◽  
Pamela Dunsmuir ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Disease Response ◽  
Pathogenesis Related

scholarly journals The 2b Silencing Suppressor of a Mild Strain of Cucumber mosaic virus Alone Is Sufficient for Synergistic Interaction with Tobacco mosaic virus and Induction of Severe Leaf Malformation in 2b-Transgenic Tobacco Plants

2011 ◽  
Vol 24 (6) ◽  
pp. 685-693 ◽  
Author(s):  
Shahid A. Siddiqui ◽  
Jari P. T. Valkonen ◽  
Minna-Liisa Rajamäki ◽  
Kirsi Lehto
Keyword(s):  
Tobacco Mosaic Virus ◽  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Transgenic Tobacco ◽  
Transgenic Tobacco Plants ◽  
Mild Strain ◽  
Tobacco Plants ◽  
2B Protein ◽  
Synergistic Relationship ◽  
Severe Leaf

Tobacco plants infected simultaneously by Tobacco mosaic virus (TMV) and Cucumber mosaic virus (CMV) are known to produce a specific synergistic disease in which the emerging leaves are filiformic. Similar developmental malformations are also caused to a lesser extent by the severe strains (e.g., Fny) of CMV alone, but mild strains (e.g., Kin) cause them only in mixed infection with TMV. We show here that transgenic tobacco plants expressing 2b protein of CMV-Kin produce filiformic symptoms when infected with TMV, indicating that only 2b protein is needed from CMV-Kin for this synergistic relationship. On the other hand, transgenic plants that express either the wild-type TMV genome or a modified TMV genome with its coat protein deleted or movement protein (MP) inactivated also develop filiformic or at least distinctly narrow leaves, while plants expressing the MP alone do not develop any malformations when infected with CMV-Kin. These results show that either TMV helicase/replicase protein or active TMV replication are required for this synergistic effect. The effect appears to be related to an efficient depletion of silencing machinery, caused jointly by both viral silencing suppressors, i.e., CMV 2b protein and the TMV 126-kDa replicase subunit.

Sign in / Sign up

Export Citation Format

Share Document