scholarly journals Intraspecies differences in cold hardiness, carbohydrate content and  -amylase gene expression of Vaccinium corymbosum during cold acclimation and deacclimation

2012 ◽  
Vol 32 (12) ◽  
pp. 1533-1540 ◽  
Author(s):  
J. H. Lee ◽  
D. J. Yu ◽  
S. J. Kim ◽  
D. Choi ◽  
H. J. Lee
Keyword(s):  
Gene Expression ◽  
Cold Acclimation ◽  
Cold Hardiness ◽  
Vaccinium Corymbosum ◽  
Carbohydrate Content ◽  
Amylase Gene

scholarly journals Study of Cold Tolerance in Blueberry Using EST Libraries, cDNA Microarrays, and Subtractive Hybridization

HortScience ◽  
2008 ◽  
Vol 43 (7) ◽  
pp. 1975-1981 ◽  
Author(s):  
Lisa J. Rowland ◽  
Anik L. Dhanaraj ◽  
Dhananjay Naik ◽  
Nadim Alkharouf ◽  
Ben Matthews ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cold Acclimation ◽  
Subtractive Hybridization ◽  
Vaccinium Corymbosum ◽  
Cdna Libraries ◽  
Woody Perennial ◽  
Floral Buds ◽  
Cold Room ◽  
Study Gene Expression ◽  
Low Temperature Exposure

To gain a better understanding of changes in gene expression associated with cold acclimation in the woody perennial blueberry (Vaccinium corymbosum L.) and ultimately use this information to develop more freeze-tolerant cultivars, a genomics approach based on the analysis of expressed sequence tags (ESTs) and microarrays was undertaken. Initially, two standard cDNA libraries, constructed using RNA from cold-acclimated (CA) and nonacclimated (NA) floral buds of the blueberry cultivar Bluecrop, were used for the generation of ≈2400 ESTs, half from each library. Putative functions were assigned to cDNAs based on homology to other genes/ESTs from GenBank. From contig analyses, 796 and 865 unique transcripts were identified from the CA and NA libraries, respectively. The most highly abundant cDNAs, that were picked many more times from one library than from the other, were identified as representing potentially differentially expressed transcripts. A cDNA microarray was constructed and used to study gene expression under cold-acclimating conditions in the field and cold room. Results indicated that the abundance of transcripts of numerous blueberry genes change during cold acclimation, including genes not found previously to be cold-responsive in Arabidopsis, and, interestingly, more transcripts were found to be upregulated under cold room conditions than under field conditions. Finally, forward and reverse subtracted cDNA libraries were prepared from ‘Bluecrop’ RNA to enrich for transcripts that are expressed at higher levels in floral buds at 400 h and at 0 h of low-temperature exposure, respectively. Many genes encoding putative transcription factors and other proteins related to signal transduction were identified from both libraries.

Changes in cold hardiness and carbohydrate content in peach ( Prunus persica ) trunk bark and wood tissues during cold acclimation and deacclimation

2017 ◽  
Vol 219 ◽  
pp. 45-52 ◽  
Author(s):  
Duk Jun Yu ◽  
Ju Young Hwang ◽  
Sun Woo Chung ◽  
Hee Duk Oh ◽  
Seok Kyu Yun ◽  
...  
Keyword(s):  
Cold Acclimation ◽  
Cold Hardiness ◽  
Prunus Persica ◽  
Carbohydrate Content

scholarly journals 194 COLD HARDINESS AND CARBOHYDRATE CONTENT OF FOUR CULTIVARS OF FIELD-GROWN SOUTHERN MAGNOLIA

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 457a-457
Author(s):  
C. L. Haynes ◽  
O. M. Lindstrom ◽  
M. A. Dirr ◽  
R. Severson
Keyword(s):  
Cold Acclimation ◽  
Cold Hardiness ◽  
Carbohydrate Content ◽  
Carbohydrate Analysis ◽  
Magnolia Grandiflora ◽  
Cold Hardy

Cold hardiness and carbohydrate content of 4 cultivars of field-grown southern magnolia (Magnolia grandiflora L.) were determined monthly during the 1992-1993 winter. Initially, `Claudia Wannamaker', `Little Gem', `Timeless Beauty', and `Victoria' had similar stem and leaf cold hardiness estimates of -6C in October. However, by February `Claudia Wannamaker' and `Victoria' stems were 6 and 3C more cold hardy than `Little Gem' and `Timeless Beauty' stems. `Claudia Wannamaker' leaves were also 6C more cold hardy than `Little Gem' and `Timeless Beauty' leaves in February. Carbohydrate analysis indicates increases in oligosaccharides during cold acclimation in fall.

Evidence for the up-regulation of stearoyl-ACP (Δ9) desaturase gene expression during cold acclimation

2004 ◽  
Vol 81 (2) ◽  
pp. 125-135 ◽  
Author(s):  
Sandra E. Vega ◽  
Alfonso H. del Rio ◽  
John B. Bamberg ◽  
Jiwan P. Palta
Keyword(s):  
Gene Expression ◽  
Cold Acclimation ◽  
Desaturase Gene ◽  
Δ9 Desaturase

Regulation of Alpha-Amylase Gene Expression

1986 ◽  
pp. 47-54
Author(s):  
H. C. Hurst ◽  
O. Hagenbüchle ◽  
U. Schibler ◽  
P. H. Shaw ◽  
D. L. Cribbs ◽  
...  
Keyword(s):  
Gene Expression ◽  
Alpha Amylase ◽  
Amylase Gene ◽  
Alpha Amylase Gene

Signal Transduction and Gene Expression During Cold Acclimation of Alfalfa

2018 ◽  
pp. 57-71
Author(s):  
Rajinder S. Dhindsa ◽  
Antonio Monroy ◽  
Lawrence Wolfraim ◽  
Guangyuan Dong
Keyword(s):  
Gene Expression ◽  
Signal Transduction ◽  
Cold Acclimation

scholarly journals Effect of Fall Fertilization on Freeze Resistance of Deciduous Versus Evergreen Azaleas

2010 ◽  
Vol 28 (4) ◽  
pp. 235-239
Author(s):  
Frank P. Henning ◽  
Timothy J. Smalley ◽  
Orville M. Lindstrom ◽  
John M. Ruter
Keyword(s):  
Nutrient Uptake ◽  
Cold Acclimation ◽  
Cold Hardiness ◽  
High Rate ◽  
Freeze Resistance ◽  
Flower Production ◽  
Moderate Rate

Abstract Plants that maintain their leaves throughout winter may respond differently to fall fertilization than deciduous plants. The effects of fall fertilization on cold hardiness, nutrient uptake, growth and flower production of evergreen versus deciduous azaleas were studied. Rhododendron canescens (Michx.) Sweet and R. × satsuki ‘Wakaebisu’ were grown in containers, outdoors in Athens, GA, under three fall fertigation regimes applied daily as 0.5 liter (0.13 gal) solutions containing: 1) 75 mg·liter−1 N from August 1 through September 29, 2) 75 mg·liter−1 N from August 1 through November 28, and 3) 125 mg·liter−1 N from August 1 through November 28. Stem freeze resistance was analyzed monthly November through March. Growth of azaleas that received 120 days of extended fertigation (August 1 through November 28) was not increased compared to azaleas that received 60 days of extended fertigation (August 1 through September 29). Growth of the two taxa did not differ in their response to fertilization treatments. The high rate of extended fertilization 125 mg·liter−1 N (from August 1 through November 28) reduced stem freeze resistance November through February, while the moderate rate of extended fertilization (75 mg·liter−1 N from August 1 through November 28) reduced azalea freeze resistance in December. Fall fertilization regimes did not produce differences in the timing of cold acclimation, or deacclimation of R. canescens and R. × satsuki. The high rate of extended fertilization promoted early budbreak of R. × satsuki and postponed flower budbreak of R. canescens. Flower production of R. canescens was not affected by fall fertilization, but the high rate of extended fertilization increased flower production of R. × satsuki compared to plants that received the moderate rate of fertilization 75 mg·liter−1 N from August 1 through September 29.

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