scholarly journals Isotope ratio laser spectroscopy to disentangle xylem-transported from locally respired CO2 in stem CO2 efflux

2021 ◽  
Author(s):  
Roberto L Salomón ◽  
Linus De Roo ◽  
Bodé Samuel ◽  
Boeckx Pascal ◽  
Kathy Steppe
Keyword(s):  
Laser Spectroscopy ◽  
Isotope Ratio ◽  
Co2 Efflux ◽  
Stem Co2 Efflux

Isotope ratio laser spectroscopy to disentangle xylem-transported from locally respired CO2 in stem CO2 efflux

2019 ◽  
Vol 39 (5) ◽  
pp. 819-830 ◽  
Author(s):  
Roberto L Salomón ◽  
Linus De Roo ◽  
Samuel Bodé ◽  
Pascal Boeckx ◽  
Kathy Steppe
Keyword(s):  
Laser Spectroscopy ◽  
Isotope Ratio ◽  
Co2 Efflux ◽  
Stem Co2 Efflux

A new automated stem CO2 efflux chamber based on industrial ultra-low-cost sensors

2019 ◽  
Author(s):  
Johannes Brändle ◽  
Norbert Kunert
Keyword(s):  
Gas Exchange ◽  
Low Cost ◽  
Climatic Conditions ◽  
Co2 Efflux ◽  
Diurnal Changes ◽  
Lowland Forest ◽  
Stem Respiration ◽  
Stem Co2 Efflux ◽  
Gas Exchange System ◽  
Controlling Processes

Abstract Tree autotrophic respiratory processes, especially stem respiration or stem CO2 efflux (Estem), are important components of the forest carbon budget. Despite the efforts to investigate the controlling processes of Estem in the last years a considerable lack in our knowledge remains on the abiotic and biotic drivers affecting Estem dynamics. It has been strongly advocated that long-term measurements would shed light into those processes. The expensive scientific instruments needed to measure gas exchange has prevented from applying Estem measurements on a larger temporal and spatial scale. Here, we present an automated closed dynamic chamber system based on inexpensive and industrially broadly applied CO2 sensors reducing the costs for the sensing system to a minimum. The CO2 sensor was cross-calibrated with a commonly used gas exchange system in the laboratory and in the field, and we found very good accordance of these sensors. We tested the system under harsh tropical climatic conditions, characterized by heavy tropical rainfall events, extreme humidity, and temperatures, in a moist lowland forest in Malaysia. We recorded Estem of three Dyera costulata trees with our prototype over various days. The variation of Estem was large among the three tree individuals and varied by 7.5-fold. However, clear diurnal changes in Estem were present in all three tree individuals. One tree showed high diurnal variation in Estem and the relationship between Estem and temperature was characterized by a strong hysteresis. The large variations found within one single tree species highlights the importance of continuous measurement to quantify ecosystem carbon fluxes.

scholarly journals Low-cost chamber design for simultaneous CO2 and O2 flux measurements between tree stems and the atmosphere

2021 ◽  
Author(s):  
Juliane Helm ◽  
Henrik Hartmann ◽  
Martin Göbel ◽  
Boaz Hilman ◽  
David Herrera ◽  
...  
Keyword(s):  
Low Cost ◽  
Co2 Flux ◽  
Co2 Efflux ◽  
Stem Respiration ◽  
Ecosystem Carbon ◽  
O2 Concentration ◽  
Flux Measurements ◽  
Stem Co2 Efflux ◽  
Tree Stem

Abstract Tree stem CO2 efflux is an important component of ecosystem carbon fluxes and has been the focus of many studies. While CO2 efflux can easily be measured, a growing number of studies have shown that it is not identical with actual in situ respiration. Complementing measurements of CO2 flux with simultaneous measurements of O2 flux provides an additional proxy for respiration, and the combination of both fluxes can potentially help getting closer to actual measures of respiratory fluxes. To date, however, the technical challenge to measure relatively small changes in O2 concentration against its high atmospheric background has prevented routine O2 measurements in field applications. Here we present a new and low-cost field-tested device for autonomous real-time and quasi-continuous long-term measurements of stem respiration by combining CO2 (NDIR based) and O2 (quenching based) sensors in a tree stem chamber. Our device operates as a cyclic closed system and measures changes in both CO2 and O2 concentration within the chamber over time. The device is battery-powered with a > 1 week power independence and data acquisition is conveniently achieved by an internal logger. Results from both field and laboratory tests document that our sensors provide reproducible measurements of CO2 and O2 exchange fluxes under varying environmental conditions.

scholarly journals Internal respiration of Amazon tree stems greatly exceeds external CO<sub>2</sub> efflux

2012 ◽  
Vol 9 (12) ◽  
pp. 4979-4991 ◽  
Author(s):  
A. Angert ◽  
J. Muhr ◽  
R. Negron Juarez ◽  
W. Alegria Muñoz ◽  
G. Kraemer ◽  
...  
Keyword(s):  
Tropical Trees ◽  
Co2 Efflux ◽  
Water Stream ◽  
New Approach ◽  
Stem Respiration ◽  
Internal Flux ◽  
Stem Co2 Efflux ◽  
Internal Co2 ◽  
Global Carbon ◽  
Net Release

Abstract. Respiration in tree stems is an important component of forest carbon balance. The rate of CO2 efflux from the stem has often been assumed to be a measure of stem respiration. However, recent work in temperate forests has demonstrated that stem CO2 efflux can either overestimate or underestimate respiration rate because of emission or removal of CO2 by transport in xylem water. Here, we studied gas exchange from stems of tropical forest trees using a new approach to better understand respiration in an ecosystem that plays a key role in the global carbon cycle. Our main questions were (1) is internal CO2 transport important in tropical trees, and, if so, (2) does this transport result in net release of CO2 respired in the roots at the stem, or does it cause the opposite effect of net removal of stem-respired CO2? To answer these questions, we measured the ratio of stem CO2 efflux to O2 influx. This ratio, defined here as apparent respiratory quotient (ARQ), is expected to equal 1.0 if carbohydrates are the substrate for respiration, and the net transport of CO2 in the xylem water is negligible. Using a stem chamber approach to quantifying ARQ, we found values of 0.66 ± 0.18. These low ARQ values indicate that a large portion of respired CO2 (~ 35%) is not emitted locally, and is probably transported upward in the stem. ARQ values of 0.21 ± 0.10 were found for the steady-state gas concentration within the stem, sampled by in-stem equilibration probes. These lower values may result from the proximity to the xylem water stream. In contrast, we found ARQ values of 1.00 ± 0.13 for soil respiration. Our results indicate the existence of a considerable internal flux of CO2 in the stems of tropical trees. If the transported CO2 is used in the canopy as a substrate for photosynthesis, it could account for up to 10% of the C fixed by the tree, and perhaps serve as a mechanism that buffers the response of the tree to changing CO2 levels. Our results also indicate, in agreement with previous work, that the widely used CO2 efflux approach for determining stem respiration is unreliable. We demonstrate here a field applicable approach for measuring the O2 uptake rate, which we suggest to be a more appropriate method to estimate stem respiration rates.

scholarly journals Implications of CO<sub>2</sub> pooling on δ<sup>13</sup>C of ecosystem respiration and leaves in Amazonian forest

2008 ◽  
Vol 5 (3) ◽  
pp. 779-795 ◽  
Author(s):  
A. C. de Araújo ◽  
J. P. H. B. Ometto ◽  
A. J. Dolman ◽  
B. Kruijt ◽  
M. J. Waterloo ◽  
...  
Keyword(s):  
Soil Moisture ◽  
Carbon Isotope ◽  
Isotope Ratio ◽  
Ecosystem Respiration ◽  
Nitrogen Concentration ◽  
Carbon Isotope Ratio ◽  
Dry Season ◽  
Small Scale ◽  
Supporting Evidence ◽  
Co2 Efflux

Abstract. The carbon isotope of a leaf (δ13Cleaf) is generally more negative in riparian zones than in areas with low soil moisture content or rainfall input. In Central Amazonia, the small-scale topography is composed of plateaus and valleys, with plateaus generally having a lower soil moisture status than the valley edges in the dry season. Yet in the dry season, the nocturnal accumulation of CO2 is higher in the valleys than on the plateaus. Samples of sunlit leaves and atmospheric air were collected along a topographical gradient in the dry season to test whether the δ13Cleaf of sunlit leaves and the carbon isotope ratio of ecosystem respired CO2 (δ13CReco) may be more negative in the valley than those on the plateau. The δ13Cleaf was significantly more negative in the valley than on the plateau. Factors considered to be driving the observed variability in δ13Cleaf were: leaf nitrogen concentration, leaf mass per unit area (LMA), soil moisture availability, more negative carbon isotope ratio of atmospheric CO2 (δ13Ca) in the valleys during daytime hours, and leaf discrimination (Δleaf). The observed pattern of δ13Cleaf might suggest that water-use efficiency (WUE) is higher on the plateaus than in the valleys. However, there was no full supporting evidence for this because it remains unclear how much of the difference in δ13Cleaf was driven by physiology or &amp;delta13Ca. The δ13CReco was more negative in the valleys than on the plateaus on some nights, whereas in others it was not. It is likely that lateral drainage of CO2 enriched in 13C from upslope areas might have happened when the nights were less stable. Biotic factors such as soil CO2 efflux (Rsoil) and the responses of plants to environmental variables such as vapor pressure deficit (D) may also play a role. The preferential pooling of CO2 in the low-lying areas of this landscape may confound the interpretation of δ13Cleaf and δ13CReco.

scholarly journals Implications of CO<sub>2</sub> pooling on δ<sup>13</sup>C of ecosystem respiration and leaves in Amazonian forest

2007 ◽  
Vol 4 (6) ◽  
pp. 4459-4506
Author(s):  
A. C. de Araújo ◽  
J. P. H. B. Ometto ◽  
A. J. Dolman ◽  
B. Kruijt ◽  
M. J. Waterloo ◽  
...  
Keyword(s):  
Soil Moisture ◽  
Carbon Isotope ◽  
Isotope Ratio ◽  
Ecosystem Respiration ◽  
Nitrogen Concentration ◽  
Carbon Isotope Ratio ◽  
Dry Season ◽  
Atmospheric Co2 ◽  
Small Scale ◽  
Co2 Efflux

Abstract. The carbon isotope of a leaf (δ13Cleaf) is generally more negative in riparian zones than in areas with low soil moisture content or rainfall input. In Central Amazonia, the small-scale topography is composed of plateaus and valleys, with plateaus generally being drier than the valley edges in the dry season. The nocturnal accumulation of CO2 is higher in the valleys than on the plateaus in the dry season. The CO2 stored in the valleys takes longer to be released than that on the plateaus, and sometimes the atmospheric CO2 concentration (ca) does not drop to the same level as on the plateaus at any time during the day. Samples of sunlit leaves and atmospheric air were collected along a topographical gradient to test whether the δ13Cleaf of sunlit leaves and the carbon isotope ratio of ecosystem respired CO2 (δ13CR) may be more negative in the valley than those on the plateau. The δ13Cleaf was significantly more negative in the valley than on the plateau. Factors considered to be driving the observed variability in δ13Cleaf were: leaf nitrogen concentration, leaf mass per unit area (LMA), soil moisture availability, more negative carbon isotope ratio of atmospheric CO2 (δ13Ca) in the valleys during daytime hours, and leaf discrimination (Δleaf). The observed pattern of δ13Cleaf suggests that water-use efficiency (WUE) may be higher on the plateaus than in the valleys. The ;13CR was more negative in the valleys than on the plateaus on some nights, whereas in others it was not. It is likely that lateral drainage of CO2 enriched in 13C from upslope areas might have happened when the nights were less stable. Biotic factors such as soil CO2 efflux (Rsoil) and the responses of plants to environmental variables such as vapor pressure deficit (D) may also play a role.

scholarly journals Variability in temperature dependence of stem CO2 efflux from Norway spruce trees

2018 ◽  
Vol 38 (9) ◽  
pp. 1333-1344 ◽  
Author(s):  
Eva Darenova ◽  
Manuel Acosta ◽  
Radek Pokorny ◽  
Marian Pavelka
Keyword(s):  
Temperature Dependence ◽  
Norway Spruce ◽  
Co2 Efflux ◽  
Stem Co2 Efflux
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