Changes in plant function and root mycobiome caused by flood and drought in a riparian tree

2020 ◽  
Vol 40 (7) ◽  
pp. 886-903 ◽  
Author(s):  
Clara Martínez-Arias ◽  
Juan Sobrino-Plata ◽  
David Macaya-Sanz ◽  
Natalie Marie Aguirre ◽  
Carmen Collada ◽  
...  
Keyword(s):  
Starch Content ◽  
Stomatal Closure ◽  
Net Photosynthesis ◽  
Climatic Conditions ◽  
Dutch Elm Disease ◽  
Host Responses ◽  
Integrative Studies ◽  
Moderate Drought ◽  
Riparian Tree ◽  
Taxonomic Groups

Abstract Under increasingly harsh climatic conditions, conservation of threatened species requires integrative studies to understand stress tolerance. Riparian Ulmus minor Mill. populations have been massively reduced by Dutch Elm disease (DED). However, resistant genotypes were selected to restore lost populations. To understand the acclimation mechanisms to the succession of abiotic stresses, ramets of five DED-tolerant U. minor genotypes were subjected to flood and subsequently to drought. Physiological and biochemical responses were evaluated together with shifts in root-fungal assemblages. During both stresses, plants exhibited a decline in leaf net photosynthesis and an increase in percentage loss of stem hydraulic conductivity and in leaf and root proline content. Stomatal closure was produced by chemical signals during flood and hydraulic signals during drought. Despite broad similarities in plant response to both stresses, root-mycobiome shifts were markedly different. The five genotypes were similarly tolerant to moderate drought, however, flood tolerance varied between genotypes. In general, flood did not enhance drought susceptibility due to fast flood recovery, nevertheless, different responses to drought after flood were observed between genotypes. Associations were found between some fungal taxonomic groups and plant functional traits varying with flood and drought (e.g. proline, chlorophyll and starch content) indicating that the thriving of certain taxa depends on host responses to abiotic stress.

Performance of interior spruce seedlings treated with abscisic acid analogs

1996 ◽  
Vol 26 (12) ◽  
pp. 2061-2070 ◽  
Author(s):  
Steven C. Grossnickle ◽  
Raymund S. Folk ◽  
Suzanne R. Abrams ◽  
David I. Dunstan ◽  
Patricia A. Rose
Keyword(s):  
Environmental Conditions ◽  
Stomatal Closure ◽  
Frozen Storage ◽  
Net Photosynthesis ◽  
Severe Drought ◽  
Bud Break ◽  
Root Temperature ◽  
Interior Spruce ◽  
Moderate Drought ◽  
Aba Analogs

This research examined the performance of interior spruce (Piceaglauca (Moench) Voss × Piceaengelmannii Parry ex Engelm.) seedlings, each group treated with one of nine abscisic acid (ABA) analogs, during the initial stages of seedling establishment under a range of environmental conditions. Interior spruce seedlings were removed from frozen storage, ABA analog treatments were immediately applied, and seedlings were tested under low root temperature or moderate drought cycle conditions. Alternatively, seedlings were removed from frozen storage and held until bud break had occurred before ABA analog treatments were applied. These seedlings were then tested under severe drought or optimum environmental conditions. ABA analog 1, followed by ABA analog 2, had the most consistent performance of the nine tested ABA analogs under all combinations of environmental test conditions. These ABA analogs reduced needle conductance for 7–9 days when seedlings were tested under low root temperature conditions with only a reduction in net photosynthesis on the first day of testing. During three successive moderate drought cycles, seedlings treated with ABA analogs 1 and 2 had partial stomatal closure, thereby increasing mean shoot water potential by around 50%. During a severe drought, ABA analog 1 caused partial stomatal closure, which allowed seedlings to maintain a mean shoot water potential of greater than −3.0 MPa and a positive net photosynthesis up to 8 days longer than control seedlings. Under optimum environmental conditions, ABA analogs 1 and 2 reduced needle conductance for up to 7 days, with net photosynthesis reduced for 1 day. Root growth was not adversely affected in seedlings treated with any of the ABA analogs prior to bud break. However, when seedlings were treated after bud break, all ABA analogs reduced growth of long roots (>4.0 cm) by approximately 60%. ABA analogs 1 and 2 delayed bud break by 4 days, when compared with control seedlings. Results are discussed in reference to the establishment process of spruce seedlings on reforestation sites.

scholarly journals Differential Morpho-Physiological and Transcriptomic Responses to Heat Stress in Two Blueberry Species

2021 ◽  
Vol 22 (5) ◽  
pp. 2481
Author(s):  
Jodi Callwood ◽  
Kalpalatha Melmaiee ◽  
Krishnanand P. Kulkarni ◽  
Amaranatha R. Vennapusa ◽  
Diarra Aicha ◽  
...  
Keyword(s):  
Heat Stress ◽  
Molecular Mechanisms ◽  
Stomatal Closure ◽  
Enrichment Analysis ◽  
Leaf Size ◽  
Vaccinium Corymbosum ◽  
Climatic Conditions ◽  
Differentially Expressed ◽  
Transcriptomic Changes ◽  
Go Terms

Blueberries (Vaccinium spp.) are highly vulnerable to changing climatic conditions, especially increasing temperatures. To gain insight into mechanisms underpinning the response to heat stress, two blueberry species were subjected to heat stress for 6 and 9 h at 45 °C, and leaf samples were used to study the morpho-physiological and transcriptomic changes. As compared with Vaccinium corymbosum, Vaccinium darrowii exhibited thermal stress adaptation features such as small leaf size, parallel leaf orientation, waxy leaf coating, increased stomatal surface area, and stomatal closure. RNAseq analysis yielded ~135 million reads and identified 8305 differentially expressed genes (DEGs) during heat stress against the control samples. In V. corymbosum, 2861 and 4565 genes were differentially expressed at 6 and 9 h of heat stress, whereas in V. darrowii, 2516 and 3072 DEGs were differentially expressed at 6 and 9 h, respectively. Among the pathways, the protein processing in the endoplasmic reticulum (ER) was the highly enriched pathway in both the species: however, certain metabolic, fatty acid, photosynthesis-related, peroxisomal, and circadian rhythm pathways were enriched differently among the species. KEGG enrichment analysis of the DEGs revealed important biosynthesis and metabolic pathways crucial in response to heat stress. The GO terms enriched in both the species under heat stress were similar, but more DEGs were enriched for GO terms in V. darrowii than the V. corymbosum. Together, these results elucidate the differential response of morpho-physiological and molecular mechanisms used by both the blueberry species under heat stress, and help in understanding the complex mechanisms involved in heat stress tolerance.

scholarly journals Prospects of practical use of new potatoes state varietal resources in the north-east Forest-Steppe of Ukraine

2019 ◽  
pp. 15-21 ◽  
Author(s):  
N.S. Kozhushko ◽  
M.M. Sakhoshko ◽  
M.G. Bashtovyi ◽  
D.V. Smilyk ◽  
V.I. Avramenko ◽  
...  
Keyword(s):  
Starch Content ◽  
Growing Season ◽  
Potato Production ◽  
Climatic Conditions ◽  
Forest Steppe ◽  
North East ◽  
The North ◽  
Significant Difference ◽  
New Varieties ◽  
Growing Conditions

According to statistic evaluation of the manifestation of signs of the economic suitability of state sort resources of 2019 registration potatoes when growing it in different agro-climatic conditions, a significant advantage of influence of the forest-steppe conditions was determined. Compared to Polesia the increase of yield rose for 4.1 t/ha or 18 % (НІР05 = 2.18, Ffact= 12.65 > F05 = 4.22) and the amount of trade potato for 22.72 ha or 20 % (Ffact = 58.87). There is also a tendency to increase the number of starch from unit of area for – 17 % and the total consumer properties for – 8 %.  However, there is a significant difference in the reduction of storability of products by 45 % and the lack of it’s resistance to macrosporiosis and late blight. It was proved that the yield of varieties for cultivation in the polesia zone by 25.7 % depended on the duration of the growing season (Ffact = 4.14 > F05  = 0.06). The relationship between yield and starch of potatoes in Polesia zone varieties  was determined – 23.6 % and in forest-steppe – 17.3 % and starch collection, respectively, 33.6 % and 28.0 %. The dependence of storability of potato which grew in the polesia zone and in forest-steppe from the amount of starch for 15 % was mathematically proved (Ffact = 2.11 > F05 = 0.17) і 11.3 % (Ffact = 1.52 > F05 = 0.24); the dependence of storability on the duration of the growing season in the Forest-Steppe conditions was confirmed(Ffact = 0.66 > F05  = 0.43). In addition, the 12 % of tasting grade of the Forest-Steppe varieties depended on the starch content amount (Ffact = 1.65 > F05 = 0.22). According to the results of the regression analysis, mathematical models have been developed to predict yields, culinary and consumer properties, and the storability of new potato varieties under different growing conditions. The project of a conveyor for the consumption of fresh product in summer  period with the involvement of varieties of early ripeness in the Forest-Steppe was presented: 80 days after planting – Sanibel, 90 days after planting – Medison, Paroli, 95 days after planting – Bazalia, Bernina, 100 days after planting – Donata, in the Polesia zone ‒ 80 days after planting – Sanibel, 90 days after planting – Paroli, 95 days after planting –Bazalia, 100 days after planting – Medison, Bernina, Donata. By distribution of varieties by predicted high content of phytonutrients there were bred varieties with red skin and creamy flesh – Bazalia and Sanibel, by high content with yellow flesh – Baltic Rosa and moderate content with white flesh – Oleksandrit, Fotynia, Ricarda. Introduction of new varieties with certain high adaptive potential of storability and content of bioactive compounds in potatoes will provide growth of potato production in the conditions of the northeast forest-steppe and increase its competitiveness in the domestic and foreign markets and will solve the problem of year-round consumption of fresh quality products.

scholarly journals Evaluation of the Effect of Irrigation on Biometric Growth, Physiological Response, and Essential Oil of Mentha spicata (L.)

Water ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 2264 ◽  
Author(s):  
Marino ◽  
Ahmad ◽  
Ferreira ◽  
Alvino
Keyword(s):  
Water Stress ◽  
Essential Oil ◽  
Water Status ◽  
Stomatal Closure ◽  
Critical Role ◽  
Net Photosynthesis ◽  
Leaf Biomass ◽  
Water Losses ◽  
Mentha Spicata ◽  
Area Index

A field experiment was performed on spearmint (Mentha spicata L.) under different irrigation regimes in a hilly area of Southern Italy. Objectives of the study include evaluating the physiological and biometrical response of mint from plant establishment up to its complete maturation, as well as the yield composition in essential oil at two different dates. Increasing levels of water stress affected later developing leaves and plant’s water status and net photosynthesis (from the beginning of stress (DAT 63), while affecting negatively the biometric response very soon and significantly from 35 DAT. Photosynthesis limitation played a critical role from DAT 53 on, namely later, in the harvest period (DAT 35–70). Under severe water stress, crop restricted water losses by modulating stomatal closure and, at harvest, showing lowered mesophyll conductance. Irrigation treatments did not affect the concentration of organic compounds, while the yield of essential oils was negatively affected by water stress due to reduced crop growth, in terms of total and leaf biomass, leaf area index (LAI) and crop height.

Fungi from the Rhynie chert: a view from the dark side

2003 ◽  
Vol 94 (4) ◽  
pp. 457-473 ◽  
Author(s):  
T. N. Taylor ◽  
S. D. Klavins ◽  
M. Krings ◽  
E. L. Taylor ◽  
H. Kerp ◽  
...  
Keyword(s):  
Fossil Record ◽  
Molecular Data ◽  
Host Responses ◽  
Dark Side ◽  
Early Devonian ◽  
Mycorrhizal Associations ◽  
Rhynie Chert ◽  
Complex Ecosystem ◽  
Taxonomic Groups ◽  
Fungal Interactions

ABSTRACTThe exquisite preservation of organisms in the Early Devonian Rhynie chert ecosystem has permitted the documentation of the morphology and life history biology of fungi belonging to several major taxonomic groups (e.g., Chytridiomycota, Ascomycota, Glomeromycota). The Rhynie chert also provides the first unequivocal evidence in the fossil record of fungal interactions that can in turn be compared with those in modern ecosystems. These interactions in the Rhynie chert involve both green algae and macroplants, with examples of saprophytism, parasitism, and mutualism, including the earliest mycorrhizal associations and lichen symbiosis known to date in the fossil record. Especially significant are several types of specific host responses to fungal infection that indicate that these plants had already evolved methods of defence similar and perhaps analogous to those of extant plants. This suggests that mechanisms underlying the establishment and sustenance of associations of fungi with land plants were well in place prior to the Early Devonian. In addition, a more complete understanding of the microbial organisms involved in this complex ecosystem can also provide calibration points for phylogenies based on molecular data analysis. The richness of the microbial community in the Rhynie chert holds tremendous potential for documenting additional fungal groups, which permits speculation about further interactions with abiotic and biotic components of the environment.

Spatio-temporal changes of drought in Romania (1961-2015), according to atmospheric circulation types and teleconnection indices

2020 ◽  
Author(s):  
Radu-Vlad Dobri ◽  
Liviu Apostol ◽  
Lucian Sfîcă ◽  
Simona Țîmpu ◽  
Ion-Andrei Niță
Keyword(s):  
Atmospheric Circulation ◽  
Standardized Precipitation Index ◽  
Atmospheric Precipitation ◽  
Climatic Conditions ◽  
Extreme Drought ◽  
Drought Severity ◽  
Severe Drought ◽  
Circulation Types ◽  
Teleconnection Indices ◽  
Moderate Drought

<p>Drought can be determined by climatic conditions (atmospheric precipitation, water supply from soil accessible to the plant, moisture and air temperature and wind speed) but is also induced by environmental aspects some of them related to anthropogenic influences.</p><p>In order to monitor the drought and its impact for Romania, four indices were analyzed in the present study (SPI (Standardized Precipitation Index), PNI (Percent of Normal Index), DI (Deciles index), and ZSI (Z-score Index)), through Meteorological Drought Monitoring software, using the total daily amount of precipitation for 27 weather stations in Romania, of which 22 stations for the period 1961-2015, 4 stations for the period 1961-2000 and one station for the period 1964-2015.</p><p>Preliminary analyzes resulting from the use of these indices were correlated with 18 GWT (Großwettertypen) atmospheric circulation types of daily mean sea level pressure (SLP). This was done using COST733 class software to evaluate the influence of large-scale mechanisms of atmospheric circulation. Also, four teleconnection indices were used, more exactly AO (Arctic Oscillation), NAO (North Atlantic Oscillation), PNA (Pacific-North American Pattern) and AAO (Antarctic Oscillation) that are recognized for their effect on climatic conditions at European scale,  <br>provided by National Oceanic and Atmospheric Administration (NOAA) – Climate Prediction Center.</p><p>Therefore, according to the types of circulation, the amount of precipitation produced in certain areas and implicitly the degree of drought severity is influenced. The types of anticyclonal circulation 13, 16 or 18, for example, which occur on average in 46 (12.7%), 14 (3.9%) , respectively 20 (5.4%) days a year, cause less precipitation as known, compared to the types of cyclonal circulation 1, 2 or 17 for example with an average of 12 (3.2%), 12 (3.2%), respectively 19 (4.3%) days a year.</p><p>In terms of drought analysis indices, according to SPI, the entire analysis interval for Iasi, located in the northeast region of Romania, was 6 years of "moderately dry", 5 years of "severely dry", and one year of "extremely dry", unlike Cluj, located in the central western region, with two years of "moderately dry", 3 years of "severely dry" and two years of "extremely dry". In Bucharest, located in the southern region of Romania there were 4 "moderately dry" years and 5 "severely dry" years. In Iasi, according to the ZSI index with the same classifications as the SPI index, there were 3 "moderately drought" years, 7 "severely drought" years and 7 "extreme drought" years, while in Cluj there were 9, 3 and respectively 6 years and in Bucharest 7, 5 and respectively 6 years with the above classification.</p><p>According to the PNI index, there were 5 "moderate drought" years in Iasi and Cluj and 6 "moderate drought" years in Bucharest. Also, there were 9 "weak drought" years in Iasi, 3 in Cluj and 5 in Bucharest.</p><p>And last but not least, according to the DI index, at all 3 stations there were 5 "extreme drought" years, 6 "severe drought" years and 5 "moderate drought" years.</p>

scholarly journals Limitations to leaf photosynthesis in field-grown grapevine under drought — metabolic and modelling approaches

2002 ◽  
Vol 29 (4) ◽  
pp. 451 ◽  
Author(s):  
João P. Maroco ◽  
M. Lucília Rodrigues ◽  
Carlos Lopes ◽  
M. Manuela Chaves
Keyword(s):  
Gas Exchange ◽  
Stomatal Closure ◽  
Net Photosynthesis ◽  
Co2 Assimilation ◽  
Quantum Yields ◽  
Vitis Vinifera L ◽  
Lower Efficiency ◽  
Photosynthetic Rates ◽  
Fructose 1,6 Bisphosphate

The effects of a slowly-imposed drought stress on gas-exchange, chlorophyll a fluorescence, biochemical and physiological parameters of Vitis vinifera L. leaves (cv. Aragonez, syn. Tempranillo) growing in a commercial vineyard (South Portugal) were evaluated. Relative to well-watered plants (predawn water potential, ΨPD = –0.13 ± 0.01 MPa), drought-stressed plants (ΨPD = –0.97 ± 0.01 MPa) had lower photosynthetic rates (ca 70%), stomatal conductance, and PSII activity (associated with a higher reduction of the quinone A pool and lower efficiency of PSII open centres). Stomatal limitation to photosynthesis was increased in drought-stressed plants relative to well-watered plants by ca 44%. Modelled responses of net photosynthesis to internal CO2 indicated that drought-stressed plants had significant reductions in maximum Rubisco carboxylation activity (ca 32%), ribulose-1,5-bisphosphate regeneration (ca 27%), and triose phosphate (triose-P) utilization rates (ca 37%) relative to well-watered plants. There was good agreement between the effects of drought on modelled biochemical parameters, and in vitro activities of key enzymes of carbon metabolism, namely Rubisco, glyceraldehyde-3-phosphate dehydrogenase, ribulose-5-phosphate kinase and fructose-1,6-bisphosphate phosphatase. Quantum yields measured under both ambient (35 Pa) and saturating CO2 (100 Pa) for drought-stressed plants were decreased relative to well-watered plants, as well as maximum photosynthetic rates measured at light and CO2 saturating conditions (three times ambient CO2 levels). Although stomatal closure was a strong limitation to CO2 assimilation under drought, comparable reductions in electron transport, CO2 carboxylation, and utilization of triose-P capacities were also adaptations of the photosynthetic machinery to dehydration that slowly developed under field conditions. Results presented in this study confirm that modelling photosynthetic responses based on gas-exchange data can be successfully used to predict metabolic limitations to photosynthesis.

Barrier Zone Formation as a Resistance Mechanism of Elms to Dutch Elm Disease

IAWA Journal ◽  
1985 ◽  
Vol 6 (1) ◽  
pp. 71-77 ◽  
Author(s):  
K.J.M. Bonsen ◽  
R.J. Scheffer ◽  
D.M. Elgersma
Keyword(s):  
Time Course ◽  
Resistance Mechanism ◽  
Dutch Elm Disease ◽  
Host Responses ◽  
Moderate Degree ◽  
Zone Formation ◽  
Ophiostoma Ulmi ◽  
Time Course Study ◽  
High Degree ◽  
Barrier Zone

Host responses of elms susceptible and resistant to Dutch elm disease were histologically examined. In a time course study the susceptible elm clone Ulmus × hollandica 'Belgica' and U. × hollandica '390', a clone which shows a high degree of resistance to non-aggressive isolates and a moderate degree of resistance to aggressive isolates of Ophiostoma ulmi, were inoculated in twig or trunk with either an aggressive or a non-aggressive isolate of O. ulmi. For purposes of comparison, the susceptible elm U. americana and the more resistant clones U. × hollandica 'Groeneveld', U. 'Lobel' and U. 'Sapporo Autumn Gold' were included. Depending on clone-isolate compatibility, infected twigs reacted by a walling off process, by barrier zone formation, or failed to resist the infection and died. Trees inoculated into the trunk reacted comparably but in the case of a compatible combination they always formed a barrier zone and the cambium never died in the year of inoculation.

scholarly journals Mian: Interactive Web-Based 16S rRNA Operational Taxonomic Unit Table Data Visualization and Discovery Platform

2018 ◽  
Author(s):  
Boyang Tom Jin
Keyword(s):  
Feature Selection ◽  
Operational Taxonomic Unit ◽  
Host Responses ◽  
Exact Test ◽  
Alpha And Beta Diversity ◽  
Sequencing Technologies ◽  
Correlation Networks ◽  
Statistical Measures ◽  
Table Data ◽  
Taxonomic Groups

ABSTRACTIn recent years, there has been strong interest in examining the microbiome and its impact on human health and the environment. By leveraging modern sequencing technologies, investigators can quickly determine the composition of a given microbial sample. At the same time, the same investigations often yield an array of categorical and numerical metadata derived from the sequenced samples such as immunohistochemical measures or locality information. Understanding how the microbiome data is associated with this external metadata is essential in developing targeted treatments for chronic diseases or proposing bacteria-modulated host responses. While many R or Python libraries and command-line tools have been developed for specific analysis purposes, there are still relatively few tools to facilitate open-ended data exploration and hypothesis generation. Here we introduce Mian, an open-source web framework to interactively visualize or run a suite of statistical and feature selection tools on the microbiome to identify important taxonomic groups in the context of any provided categorical or numerical metadata. Visualizations include boxplots, correlation networks, and PCA or NMDS scatterplots. Tools include Fisher’s Exact Test, Boruta feature selection, alpha and beta diversity, and differential and correlational analysis. Mian supports multiple standard representations of the OTU table as input and optionally subsamples the data during the upload process. Users can also filter and aggregate the OTU table at different taxonomic levels and dynamically adjust analysis parameters to see how the visualizations, results, and statistical measures change in real-time. Mian is freely available at: miandata.org

scholarly journals The relationship of agroclimatic factors and the yield of potato varieties in the conditions of the near Moscow region

2021 ◽  
Vol 2 (44) ◽  
pp. 21-21
Author(s):  
Alexander Saakian ◽  
◽  
Keyword(s):  
Starch Content ◽  
Organic Fertilizer ◽  
Weather Conditions ◽  
Climatic Conditions ◽  
Hydrothermal Coefficient ◽  
Potato Varieties ◽  
Yield And Quality ◽  
Relationship Of ◽  
The Relationship

In 2005-2015. studied the effect of liquid organic fertilizer BIOOD-1 as foliar feeding on the yield and quality of various potato varieties in changing meteorological conditions. According to the results of the experiments, it was established that the most stable yields under various weather conditions were shown by the potatoes of the mid-season variety Bryansk delicacy. The low starch content of varieties Nevsky, Udacha was noted in years of high humidity, but with a lack of moisture, the starch content of these varieties increased in the background variant and in the experiment with organic fertilization. Keywords: CLIMATIC CONDITIONS, HYDROTHERMAL COEFFICIENT, MEAN SENIOR PRECIPITATION, POTATO, YIELD, STARCH, HUMIC FERTILIZERS

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