scholarly journals Differential Response to Abiraterone Acetate and Di-n-butyl Phthalate in an Androgen-Sensitive Human Fetal Testis Xenograft Bioassay

2013 ◽  
Vol 138 (1) ◽  
pp. 148-160 ◽  
Author(s):  
Daniel J. Spade ◽  
Susan J. Hall ◽  
Camelia M. Saffarini ◽  
Susan M. Huse ◽  
Elizabeth V. McDonnell ◽  
...  
Keyword(s):  
Differential Response ◽  
Abiraterone Acetate ◽  
Fetal Testis ◽  
Butyl Phthalate

scholarly journals Do Phthalates Affect Steroidogenesis by the Human Fetal Testis? Exposure of Human Fetal Testis Xenografts to Di-n-Butyl Phthalate

2012 ◽  
Vol 97 (3) ◽  
pp. E341-E348 ◽  
Author(s):  
R. T. Mitchell ◽  
A. J. Childs ◽  
R. A. Anderson ◽  
S. van den Driesche ◽  
P. T. K. Saunders ◽  
...  
Keyword(s):  
Fetal Testis ◽  
Butyl Phthalate

scholarly journals Comparative Effects of Di( n -Butyl) Phthalate Exposure on Fetal Germ Cell Development in the Rat and in Human Fetal Testis Xenografts

2015 ◽  
Vol 123 (3) ◽  
pp. 223-230 ◽  
Author(s):  
Sander van den Driesche ◽  
Chris McKinnell ◽  
Ana Calarrão ◽  
Laura Kennedy ◽  
Gary R. Hutchison ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cell Development ◽  
Germ Cell Development ◽  
Fetal Testis ◽  
Phthalate Exposure ◽  
Butyl Phthalate ◽  
Fetal Germ Cell

scholarly journals Abnormal Leydig Cell Aggregation in the Fetal Testis of Rats Exposed to Di (n-Butyl) Phthalate and Its Possible Role in Testicular Dysgenesis

Endocrinology ◽  
2005 ◽  
Vol 146 (2) ◽  
pp. 613-623 ◽  
Author(s):  
I. Kim Mahood ◽  
Nina Hallmark ◽  
Chris McKinnell ◽  
Marion Walker ◽  
Jane S. Fisher ◽  
...  
Keyword(s):  
Leydig Cell ◽  
Cell Aggregation ◽  
Fetal Testis ◽  
Testicular Dysgenesis ◽  
Butyl Phthalate

scholarly journals Reconstitution of rat fetal testis during the masculinisation programming window induces focal dysgenesis consistent with testicular dysgenesis syndrome

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Ellie Smart ◽  
Joni Macdonald ◽  
Lee B. Smith ◽  
Rod T. Mitchell
Keyword(s):  
Testicular Dysgenesis Syndrome ◽  
Fetal Testis ◽  
Specific Effects ◽  
Fetal Rat ◽  
Testicular Dysgenesis ◽  
Seminal Vesicle Weight ◽  
Consistent Feature ◽  
Butyl Phthalate ◽  
Testis Tissue

Abstract Focal dysgenesis is a consistent feature of testicular dysgenesis syndrome (TDS) in humans. Rodent studies show that perturbation of androgens (e.g. following phthalate exposure) during a fetal masculinisation programming window (MPW) predisposes to a TDS phenotype. This study aimed to determine whether dissociation and reconstitution of rat fetal testis tissue during the MPW can be used to model and manipulate seminiferous cord development, including induction of focal dysgenesis, as described in TDS. Dissociated fetal rat testes were xenotransplanted subcutaneously into recipient mice for 4 weeks. Transplanted mice were treated with vehicle or di-n-butyl-phthalate (DBP, a plasticising chemical known to induce testicular dysgenesis in vivo in rats). Testosterone production by the transplants was measured in recipient mice and immunofluorescence was performed on the retrieved transplants to identify features consistent with focal testicular dysgenesis. Re-aggregation of rat fetal testis tissue xenotransplants during the MPW results in reconstitution of seminiferous cords. Features of focal testicular dysgenesis were present in re-aggregated testis, including ectopic Sertoli cells and intratubular Leydig cells (ITLCs). DBP exposure of recipient mice reduced androgen-dependent seminal vesicle weight (8.3 vs 26.7 mg; p < 0.05), but did not enhance features of focal dysgenesis including number of ITLCs (0.07 vs 0.10 cells/mm2; p > 0.05). We conclude that seminiferous cord reformation during the MPW results in development of focal dysgenesis. The system may be used to separate specific effects (e.g. androgen suppression) of individual chemical exposures from other mechanisms that may be conserved in TDS.

The Differential Response Experience: National and State Portraits in Innovation

2007 ◽  
Author(s):  
Caren Kaplan ◽  
David Thompson ◽  
Kathryn Sapp ◽  
Lisa Merkel-Holguin
Keyword(s):  
Differential Response
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