scholarly journals Acrylonitrile-Induced Oxidative Stress and Oxidative DNA Damage in Male Sprague-Dawley Rats

2009 ◽  
Vol 111 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Xinzhu Pu ◽  
Lisa M. Kamendulis ◽  
James E. Klaunig
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals Oxidative stress, DNA damage, and antioxidant enzyme activity induced by hexavalent chromium in Sprague-Dawley rats

2009 ◽  
Vol 24 (1) ◽  
pp. 66-73 ◽  
Author(s):  
Anita K. Patlolla ◽  
Constance Barnes ◽  
Clement Yedjou ◽  
V. R. Velma ◽  
Paul B. Tchounwou
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Enzyme Activity ◽  
Antioxidant Enzyme ◽  
Hexavalent Chromium ◽  
Antioxidant Enzyme Activity ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals JAK Inhibition Prevents DNA Damage and Apoptosis in Testicular Ischemia-Reperfusion Injury via Modulation of the ATM/ATR/Chk Pathway

2021 ◽  
Vol 22 (24) ◽  
pp. 13390
Author(s):  
Farah Khashab ◽  
Farah Al-Saleh ◽  
Nora Al-Kandari ◽  
Fatemah Fadel ◽  
May Al-Maghrebi
Keyword(s):  
Dna Damage ◽  
Reperfusion Injury ◽  
Oxidative Dna Damage ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Specific Inhibitor ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Testicular Ischemia ◽  
Ap Sites

Testicular ischemia reperfusion injury (tIRI) causes oxidative stress-induced DNA damage leading to germ cell apoptosis (GCA). The aim of the study is to establish a direct link between JAK2 activation and the DNA damage response (DDR) signaling pathways and their role in tIRI-induced GCA using AG490, a JAK2 specific inhibitor. Male Sprague Dawley rats (n = 36) were divided into three groups: sham, unilateral tIRI and tIRI + AG490 (40 mg/kg). During tIRI, augmentation in the phosphorylation levels of the JAK2/STAT1/STAT3 was measured by immunohistochemistry. Observed spermatogenic arrest was explained by the presence of considerable levels of DSB, AP sites and 8OHdG and activation of caspase 9, caspase 3 and PARP, which were measured by colorimetric assays and TUNEL. The ATM/Chk2/H2AX and ATR/Chk1 pathways were also activated as judged by their increased phosphorylation using Western blot. These observations were all prevented by AG490 inhibition of JAK2 activity. Our findings demonstrate that JAK2 regulates tIRI-induced GCA, oxidative DNA damage and activation of the ATM/Chk2/H2AX and ATR/Chk1 DDR pathways, but the cell made the apoptosis decision despite DDR efforts.

scholarly journals Curcumin Modulates Nitrosative Stress, Inflammation and DNA Damage and Protects against Ochratoxin A-Induced Hepatotoxicity and Nephrotoxicity in Rats

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1239
Author(s):  
Consiglia Longobardi ◽  
Sara Damiano ◽  
Emanuela Andretta ◽  
Francesco Prisco ◽  
Valeria Russo ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Dna Damage ◽  
Ochratoxin A ◽  
Oxidative Dna Damage ◽  
Nitrosative Stress ◽  
Antioxidant Activities ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Natural Polyphenol ◽  
Oxide Synthase

Ochratoxin A (OTA) is a fungal toxin of critical concern for food safety both for human health and several animal species, also representing a cancer threat to humans. Curcumin (CURC) is a natural polyphenol that has anti-apoptotic, anti-inflammatory, and antioxidant effects. The aim of this study was to investigate the cytoprotective effect of CURC against OTA-induced nephrotoxicity and hepatotoxicity through the study of the nitrosative stress, pro-inflammatory cytokines, and deoxyribonucleic acid (DNA) damage. Sprague Dawley rats were daily treated with CURC (100 mg/kg b.w.), OTA (0.5 mg/kg b.w), or CURC with OTA by oral gavage for 14 days. Our results demonstrated that OTA exposure was associated with significant increase of pro-inflammatory and DNA oxidative-damage biomarkers. Moreover, OTA induced the inducible nitric oxide synthase, (iNOS) resulting in increased nitric oxide (NO) levels both in kidney and liver. The co-treatment OTA + CURC counteracted the harmful effects of chronic OTA treatment by regulating inflammation, reducing NO levels and oxidative DNA damage in kidney and liver tissues. Histology revealed that OTA + CURC treatment determinates mainly an Iba1+ macrophagic infiltration with fewer CD3+ T-lymphocytes in the tissues. In conclusion, we evidenced that CURC exerted cytoprotective and antioxidant activities against OTA-induced toxicity in rats.

Serum of 8-OHdG as a potential biomarker of oxidative DNA damage in workers exposed to harmful working environments

2020 ◽  
pp. 783-783
Author(s):  
I. A. Umnyagina ◽  
L. A. Strakhova ◽  
T. V. Blinova
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Blood Serum ◽  
Oxidative Dna Damage ◽  
Working Environment ◽  
Working Environments ◽  
Potential Biomarker ◽  
High Level ◽  
Damage Marker

In the blood serum of 70% individuals exposed to harmful factors of the working environment, a high level of oxidative stress and the DNA damage marker 8-Hydroxy-2’-Deoxyguanosine (8-OHdG) were detected.

Effects of Dietary Supplements on Space Radiation-Induced Oxidative Stress in Sprague-Dawley Rats

2004 ◽  
Vol 162 (5) ◽  
pp. 572-579 ◽  
Author(s):  
Jun Guan ◽  
X. Steven Wan ◽  
Zhaozong Zhou ◽  
Jeffrey Ware ◽  
Jeremiah J. Donahue ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dietary Supplements ◽  
Space Radiation ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Radiation Induced
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