scholarly journals Expression of Concern: “The Dual Roles of c-Jun NH2-Terminal Kinase Signaling in Cr(VI)-Induced Apoptosis in JB6 Cells”

2020 ◽  
Vol 175 (1) ◽  
pp. 145-145
Author(s):  
Young-Ok Son ◽  
John Andrew Hitron ◽  
Senping Cheng ◽  
Amit Budhraja ◽  
Zhuo Zhang ◽  
...  
Keyword(s):  
Kinase Signaling ◽  
Dual Roles ◽  
Jb6 Cells ◽  
Induced Apoptosis

scholarly journals The Dual Roles of c-Jun NH2-Terminal Kinase Signaling in Cr(VI)-Induced Apoptosis in JB6 Cells

2010 ◽  
Vol 119 (2) ◽  
pp. 335-345 ◽  
Author(s):  
Y.-O. Son ◽  
J. A. Hitron ◽  
S. Cheng ◽  
A. Budhraja ◽  
Z. Zhang ◽  
...  
Keyword(s):  
Kinase Signaling ◽  
Dual Roles ◽  
Jb6 Cells ◽  
Induced Apoptosis

scholarly journals Syntaxin-17-Dependent Mitochondrial Dynamics is Essential for Protection Against Oxidative-Stress-Induced Apoptosis

Antioxidants ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 522 ◽  
Author(s):  
Wang ◽  
Xiao ◽  
Huang ◽  
Liu
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Dynamics ◽  
Mitochondrial Fission ◽  
Autophagic Flux ◽  
Wild Type ◽  
Dual Roles ◽  
Defective Mutant ◽  
U2os Cells ◽  
Induced Apoptosis

In this study, cell death induced by the oxidant tert-butylhydroperoxide (tBH) was observed in U2OS cells; this phenotype was rescued by Syntaxin 17 (STX17) knockout (KO) but the mechanism is unknown. STX17 plays dual roles in autophagosome–lysosome fusion and mitochondrial fission. However, the contribution of the two functions of STX17 to apoptosis has not been extensively studied. Here, we sought to dissect the dual roles of STX17 in oxidative-stress-induced apoptosis by taking advantage of STX17 knockout cells and an autophagosome–lysosome fusion defective mutant of STX17. We generated STX17 knockout U2OS cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system and the STX17 knockout cells were reconstituted with wild-type STX17 and its autophagosome–lysosome fusion defective mutant. Autophagy was assessed by autophagic flux assay, Monomer red fluorescent protein (mRFP)–GFP–LC3 assay and protease protection assay. Golgi, endoplasmic reticulum (ER)/ER–Golgi intermediate compartment (ERGIC) and mitochondrial dynamics were examined by staining the different indicator proteins. Apoptosis was evaluated by caspase cleavage assay. The general reactive oxygen species (ROS) were detected by flow cytometry. In STX17 complete knockout cells, sealed autophagosomes were efficiently formed but their fusion with lysosomes was less defective. The fusion defect was rescued by wild-type STX17 but not the autophagosome–lysosome fusion defective mutant. No obvious defects in Golgi, ERGIC or ER dynamics were observed. Mitochondria were significantly elongated, supporting a role of STX17 in mitochondria fission and the elongation caused by STX17 KO was reversed by the autophagosome–lysosome fusion defective mutant. The clearance of protein aggregation was compromised, correlating with the autophagy defect but not with mitochondrial dynamics. This study revealed a mixed role of STX17 in autophagy, mitochondrial dynamics and oxidative stress response. STX17 knockout cells were highly resistant to oxidative stress, largely due to the function of STX17 in mitochondrial fission rather than autophagy.

scholarly journals Variations in IB1/JIP1 Expression Regulate Susceptibility of  -Cells to Cytokine-Induced Apoptosis Irrespective of C-Jun NH2-Terminal Kinase Signaling

Diabetes ◽  
2003 ◽  
Vol 52 (10) ◽  
pp. 2497-2502 ◽  
Author(s):  
Z. Ling ◽  
M. Van de Casteele ◽  
J. Dong ◽  
H. Heimberg ◽  
J.-A. Haefliger ◽  
...  
Keyword(s):  
Kinase Signaling ◽  
Induced Apoptosis

scholarly journals Silibinin Up-regulates DNA-Protein Kinase-dependent p53 Activation to Enhance UVB-induced Apoptosis in Mouse Epithelial JB6 Cells

2005 ◽  
Vol 280 (21) ◽  
pp. 20375-20383 ◽  
Author(s):  
Sivanandhan Dhanalakshmi ◽  
Chapla Agarwal ◽  
Rana P. Singh ◽  
Rajesh Agarwal
Keyword(s):  
Protein Kinase ◽  
P53 Activation ◽  
Jb6 Cells ◽  
Induced Apoptosis
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