scholarly journals Effects of exogenous α-amylases, glucoamylases and proteases on ruminal in vitro dry matter and starch digestibility, gas production and volatile fatty acids of mature dent corn grain

2020 ◽  
Author(s):  
Felipe X Amaro ◽  
Donghyeon Kim ◽  
Mariele C N Agarussi ◽  
Vanessa P Silva ◽  
Tatiane Fernandes ◽  
...  
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
Block Design ◽  
Gas Production ◽  
Starch Digestibility ◽  
In Vivo Studies ◽  
Random Factor ◽  
Dent Corn ◽  
Corn Grain

Abstract Two separate experiments were carried out to evaluate the effects of incremental doses of 10 exogenous endo-acting α-amylase and exo-acting glucoamylase; 1LAT (bacterial α-amylase), 2AK, 3AC, 4Cs4, 5Trga, 6Afuga, 7Fvga, and 10Tg (fungal α-amylases, glucoamylases and α-glucosidase), 8Star and 9Syn (fungal amylase-mixtures; Exp. 1) and 3 exogenous proteases; 11P14L, 12P7L, and 13P30L (bacterial proteases; Exp. 2) on in vitro dry matter digestibility (IVDMD) and in vitro starch digestibility (IVSD) of mature dent corn grain using a batch culture system. Incremental doses of the exogenous enzymes (0, 0.25, 0.50, 0.75 and 1.00 mg/g of dried substrate) were applied directly to the substrate (0.5 g of ground corn, 4 mm) in sextuplicate (Exp. 1) or quadruplicate (Exp. 2) within F57 filter bags, which were incubated at 39°C in buffered rumen fluid for 7 h. Rumen fluid was collected 2-3 h after the morning feeding from 3 lactating dairy cows and pooled. Cows were consuming a mid-lactation total mixed ration (TMR; 1.60 Mcal/kg DM and 15.4%; net energy of lactation and crude protein, respectively). Three independent runs were carried out for each experiment. Data were analyzed as a randomized complete block design using run as the blocking factor. Dose was used as a fixed factor while run was considered a random factor. Linear, quadratic, and cubic orthogonal contrasts were also tested. In Exp. 1, enzymes 2AK, 3AC and 10Tg did not increase (P > 0.10) IVDMD and IVSD, whereas 0.25 mg of enzymes 1LAT, 5Trga and 8Star increased (P < 0.01) IVDMD by 23, 47 and 62% and IVSD by 35, 41 and 58%, respectively, compared with the control. Enzymes 4Cs4, 6Afuga, 7Fvga and 9Syn linearly increased IVDMD and IVSD (P < 0.01). Greatest increases in IVDMD (82.9%) and IVSD (85.9%) resulted with 1 mg of 6Afuga compared to control. In Exp. 2, the lowest dose of exogenous proteases 11P14L and 12P7L increased (P < 0.01) IVDMD by 98 and 87% and IVSD by 57 and 64%, respectively, whereas the highest dose of 13P30L increased (P = 0.02) IVDMD by 44.8% and IVSD by 30%, relative to the control. In conclusion, IVSD and IVDMD were increased by one α-amylase, certain glucoamylases and all proteases tested, with the glucoamylase 6Afuga in Exp. 1 and the neutral protease12P7L in Exp. 2, increasing IVDMD and IVSD to the greater extents. Future in vivo studies are required to validate these findings before these enzyme additives can be recommended for improving the digestibility of mature dent corn grain.

scholarly journals PSVI-42 Effect of increasing concentrations of aflatoxins (AFB1) on rumen gas production (GP), dry matter (DM) disappearance and fermentation parameters using the in vitro ANKOM gas technique

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 194-195
Author(s):  
Jean-Philippe Marden ◽  
Virginie Marquis ◽  
Kheira Hadjeba Medjdoub ◽  
Marine Lacombe
Keyword(s):  
Feed Efficiency ◽  
Dry Matter ◽  
In Vitro Model ◽  
Rumen Fluid ◽  
Gas Production ◽  
Corn Silage ◽  
Vitro Model ◽  
Fermentation Parameters ◽  
Cheese Cloth

Abstract Aflatoxins are secondary metabolites produced by Aspergillus species known to be the most prevalent contaminants in feedstuffs. In ruminants, contaminated AFB1 feeds usually exhibit symptoms including reduced feed efficiency and milk production and decreased appetite. The objective of this study was to investigate the effects of different concentrations of AFB1 on rumen fermentation parameters by using the ANKOM gas production protocol. Rumen fluid was collected from a cannulated dry dairy cow, filtered with cheese-cloth and diluted (1:1) with a standard buffer. Triplicates of 75 mL flasks were fed 0,75g of feed (79% corn silage, 15% alfalfa and 6% concentrates) and inoculated with 0 (blank), 0,2, 0,5, 1 and 2 ppm of AFB1. Flasks were placed in a rotating incubation at 39°C for 96h and connected to ANKOM GP system. After 96h of incubation, the contents of each flask were centrifuged. Supernatants were analyzed for total VFA and AFB1 while precipitates were dried at 104°C for DM disappearance. The experimentation was repeated weekly 3 times and named wk1, 2 and 3. Statistical analysis was done by SPSS using a univariate model. Results showed no significant differences on GP max at 96h among AFB1 concentrations. Only wk 1 showed that higher AFB1 concentration (2 ppm) decreased significantly (P < 0,05) DM disappearance (- 8,2 pts) when compared to the blank. Total VFA contents (75,0 ± 1,6 mM) were not affected by AFB1. Wk 2 and 3 did not show any difference neither on DM disappearance nor on VFA (89,1 ± 1,6 mM; 110,2 ± 4,8 mM). It can be concluded that our in vitro model, GP did not reflect DM disappearance and it can be put forward that rumen fluid with low total VFA concentrations (≤ 75 mM) could be more sensible to AFB1 challenge.

Comparison of inocula from sheep and cattle for thein vitrogas production technique under tropical conditions

1999 ◽  
Vol 1999 ◽  
pp. 151-151 ◽  
Author(s):  
I.C.S. Bueno ◽  
A.L. Abdalla ◽  
S.L.S. Cabral Filho ◽  
D.M.S.S. Vitti ◽  
E. Owen ◽  
...  
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
Small Ruminants ◽  
Gas Production ◽  
Barley Straw ◽  
Tropical Conditions ◽  
Production Techniques ◽  
Microbial Mass

The use of small ruminants, such as sheep, in metabolism studies is more convenient as handling problems are reduced and their maintenance costs are lower, in comparison with cattle. However in vivo digestibility estimates obtained at maintenance are known to differ between these two species. With the increased use ofin vitrogas production techniques, to evaluate ruminant feedingstuffs, it is of great importance to identify whether the species from which the rumen fluid inoculum is obtained has a significant influence on the results obtained.Rumen fluid samples were obtained from a non-lactating Holstein cow (C) and six wether sheep (S) offered the same diet (80 % tropical grass and 20 % dairy concentrate) and prepared so as to have similar dry matter (DM) contents and therefore potentially the microbial mass. Nine substrates (two tropical grasses 1-2, tropical alfalfa 3, barley straw 4, and five temperate grasses 5-9) were examined.

scholarly journals Comparison of three different rumen fluid as a source of inoculum to evaluate in vitro gas production and digestibility of elephant grass-concentrate mixture

2021 ◽  
Vol 888 (1) ◽  
pp. 012076
Author(s):  
H Soetanto ◽  
RM Aprilia ◽  
MS Pramita ◽  
I Banna
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Gas Production ◽  
Elephant Grass ◽  
Buffer Solution ◽  
Cattle Breeds ◽  
In Vitro Gas Production ◽  
Concentrate Mixture

Abstract This study aimed at elucidating the use of three different rumen fluid (RF) of indigenous cattle breeds i.e. Bali, Madura and Crossbred Ongole immediately after slaughtered at abattoir to evaluate the nutritive value of elephant grass( EG) -concentrate mixture using a standard in vitro gas production (IVGP) technique. Approximately 500 mg feed dry matter/syringe was added with 50 ml RF-buffer solution and incubated in a 39 0C water bath for 48 hours where gas production was observed at time intervals. Following termination of incubation the content was transferred into tare glass crucible to measure rumen dry matter (RDMD) and organic matter (ROMD) digestibility. The results showed that there was no significant different (P>0.05) in gas production parameters. In contrast, RDMD and ROMD differed significantly (P<0.01) among cattle breeds. RF from OCB resulted in the highest IVGP, RDMD and ROMD as compared with other RF sources. In conclusion, the use of RF from abattoir for IVGP measurement can be warranted using the same source of RF. The highest values resulted from OCB suggests that the abundance and variation in rumen microbiota may exist among cattle breeds.

scholarly journals Determination of in vitro rumen digestibility and potential feed value of tiger nut varieties

2021 ◽  
Vol 50 (5) ◽  
Author(s):  
T. Ayaşan ◽  
E. Sucu ◽  
I. Ülger ◽  
H. Hızlı ◽  
P. Cubukcu ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Nutritive Value ◽  
Energy Content ◽  
Rumen Fluid ◽  
Gas Production ◽  
Time Dependent ◽  
In Vivo Studies ◽  
In Vitro Digestibility ◽  
In Vitro Gas Production

Tiger nut (Cyperus esculentus L.), or chufa, is a plant that is found in nature and is cultivated for its edible tubers. The purpose of this study was to determine the chemical composition, nutritive value, and in vitro digestibility of three tiger nut varieties using the in vitro gas production technique. These varieties were Sarışeker (yellow), Introduction 1, and Balyumru (brown). Rumen fluid was obtained from two cannulated Holstein animals. Time-dependent in vitro gas production was monitored at 3, 6, 9, 12, 24, 48, 72, and 96 hours of incubation. The varieties differed in dry matter (DM), crude ash (CA), ether extract (EE), neutral detergent fibre (NDF), and non-fibre carbohydrate (NFC) content (P <0.05). They also differed in the instantaneous volume of gas produced and in time-dependent gas production. Balyumru produced more gas at the onset of incubation than Introduction 1 and Sarışeker. However, over time, the gas produced by digestion of Introduction 1 exceeded the other two varieties. The amounts of gas produced at each time-point were intercorrelated. It is recommended that these results should lead to further evaluation in in vivo studies. Keywords: chemical composition, energy content, in vitro gas production

scholarly journals 183 Effect of fermentation temperature on in vitro digestibility of a finishing diet

2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 62-63
Author(s):  
Miranda K Stotz ◽  
Sebastian E Mejia-Turcios ◽  
Andrea M Osorio ◽  
Nadira Espinoza ◽  
Philip M Urso ◽  
...  
Keyword(s):  
Rumen Fluid ◽  
Block Design ◽  
Random Effect ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Ph Change ◽  
Fermentation Rate ◽  
N Concentration ◽  
Effect Of Treatment

Abstract Heat stress (HS) has been indicated to increase ruminal temperature, increase digestibility, and reduce ruminal pH of steers fed high concentrate diets. However, it is unclear if this effect is the result of greater fermentation rate, slower passage rate, or a combination. The effect of ruminal incubation temperature on substrate digestibility and rate of fermentation were evaluated. Four cannulated British-crossbreed steers (BW = 520 kg ± 30 kg) consuming an 87% (DM) concentrate diet were utilized as rumen fluid donors in a randomized complete block design with two incubation treatments (CON=39°C and HS=42°C). Within block, duplicate 125 mL serum bottles containing 200 mg of dietary substrate were inoculated with a 2:1 buffer:ruminal fluid mixture and incubated for 24 h to measure total gas production, fermentation rate, fermentation lag, pH, and ammonia-N concentration. In vitro organic matter digestibility (IVOMD) was measured separately in 100 mL centrifuge tubes. Data were analyzed using the MIXED procedure of SAS using the fixed effect of treatment and random effect of rumen fluid donor (block). There was no treatment effect on total gas production (P = 0.92) or fermentation rate (P = 0.11); however, HS began fermenting substrate sooner than CON (P < 0.005). There was a significant effect of treatment on IVOMD where HS was greater compared to CON (79.3 vs. 70.4%; P = 0.05). Final pH and relative pH change were not different (P ≥ 0.25) likely due to buffering capacity of the in vitro technique Ammonia-N concentration was greater for HS than CON (7.92 vs. 5.33 mM; P < 0.05) and may indicate a temperature effect on ruminal nitrogen availability.In conclusion, it does not appear that incubating at a greater temperature affected the fermentation rate but likely induced a change in fermentation kinetics, which may have contributed to the greater overall IVOMD

scholarly journals Screening of Cyanide-Utilizing Bacteria from Rumen and In Vitro Evaluation of Fresh Cassava Root Utilization with Pellet Containing High Sulfur Diet

2020 ◽  
Author(s):  
Rittikeard Prachumchai ◽  
Anusorn Cherdthong ◽  
Metha Wanapat
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
In Vitro Study ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Cassava Root ◽  
Randomized Design ◽  
Completely Randomized Design

The current work aimed to screen the ruminal cyanide-utilizing bacteria and evaluate the influence of fresh cassava root (FCR) and pellets containing high sulfur (PELFUR) on cyanide content, kinetics of gas, in vitro degradability, and ruminal fermentation. The experiment was conducted in a Completely randomized design (CRD) for a screening of cyanide-utilizing bacteria and the dietary treatments were the level of cyanide at 0, 150, 300, and 450 ppm. A 5 &times; 3 factorial arrangement in a Completely randomized design was used for in vitro study. Factor A was the level of FCR at 0, 260, 350, 440, and 530 g/kg of 0.5 g dry matter (DM) substrate, and factor B was the level of PELFUR at 0, 15, and 30 g/kg DM substrate. Adding different doses of cyanide significantly affected cyanide-utilizing rumen bacterial growth (p &lt; 0.05). Increasing the concentration of cyanide from 0 to 150 and 150 to 300 ppm, resulted in an increase in cyanide-utilizing rumen bacteria of 38.2% and 15.0%, respectively. Increasing the FCR level to more than 260 g/kg of 0.5 g substrate could increase cumulative gas production (p &lt; 0.05), whereas increasing doses of PELFUR from 15 to 30 g/kg increased the cumulative gas production when compared with that of 0 g/kg PELFUR (p &lt; 0.05). Cyanide concentration in rumen fluid decreased with PELFUR (p &lt; 0.05) supplementation. Degradability of in vitro dry matter and organic matter following incubation increased at 12 and 24 h due to PELFUR supplementation with FCR and increased additionally with 15 g/kg PELFUR (p &lt; 0.05) in 440 g/kg FCR. Proportions of the total volatile fatty acids, acetic acid (C2), propionic acid (C3), and butyric acid, as well as the ratio of C2 to C3 among supplementations with FCR (p &lt; 0.05) were significantly different. As the proportion of FCR increased to 530 g/kg of the substrate, the volume of C3 increased by 14.6%. This is the first finding of bacteria in the rumen capable of utilizing cyanide, and cyanide might function as a nitrogen source for bacterial cell synthesis. Inclusion of FCR of 530 g/kg with 30 g/kg PELFUR could increase the cumulative gas production, the bacterial population, the in vitro degradability, the proportion of C3, and the rate of the disappearance of cyanide.

scholarly journals Effect of fermentation on mixed rumen contents and jackfruit peel using Aspergillus oryzae on in vitro gas production and digestibility

2022 ◽  
Vol 335 ◽  
pp. 00048
Author(s):  
Mashudi Mashudi ◽  
Wahyuni Nurmawati
Keyword(s):  
Organic Matter ◽  
Analysis Of Variance ◽  
Aspergillus Oryzae ◽  
Dry Matter ◽  
Block Design ◽  
Gas Production ◽  
Dry Matter Digestibility ◽  
Randomized Block Design ◽  
In Vitro Gas Production

The objective of this research was to investigate the effect of Aspergillus oryzae on fermentation of mixture of rumen contents and jackfruit peel on in vitro gas production and digestibility. The method used in this study was an experiment using a randomized block design (RBD) of 4 treatments and 3 replications. The treatments including of T0 = 50% rumen contents + 50% jackfruit peel, T1 = 40 % rumen contents + 60% jackfruit peel + 0,4% Aspergillus oryzae, T2 = 30% rumen contents + 70% jackfruit peel + 0,4% Aspergillus oryzae, T3 = 20% rumen contents + 80% jackfruit peel + 0,4% Aspergillus oryzae. Variables observed were gas production, dry matter digestibility (DMD), and organic matter digestibility (OMD). Data were analyzed by using Analysis of Variance (ANOVA) from Randomized Block Design, if there were significant effect between the treatments then tested with least significant different (LSD). The result showed that fermentation of mixture of rumen contents and jackfruit peel have highly significant effect (P<0.01) on gas production, and significant effect (P<0.05) on DMD and OMD. Gas production, DMD and OMD of fermented mixture of rumen contents and jackfruit peel are higher than control without fermentation. It is concluded that the higher jackfruit peels the higher gas production, DMD and OMD.

scholarly journals PSIX-10 In vitro dry matter digestibility, gas production and pH of silages of several maize hybrids

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 415-416
Author(s):  
Pedro Queiroz ◽  
Israel Alves Filho ◽  
Sergio Pereira Junior ◽  
Flavia Scarpino van Cleef ◽  
Jane Ezequiel ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Gas Production ◽  
Measured Data ◽  
Maize Hybrids ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Completely Randomized Design

Abstract The objective of this study was to determine the in vitro dry matter digestibility (IVDMD), gas production and pH of silages of 10 maize hybrids in Iturama, Brazil (XB 6012 BT, BM 709 VT PRO2, BM 815 VT PRO2, DKB 363 VT PRO3, AG 8740 VT PRO3, SYN 555 VIP3, SX 8555 VIP3, SX 7005 VIP3, DKB 390 VT PRO3, and RB 9789 VIP3). The maize was ensiled in experimental mini silos in a completely randomized design with 3 repetitions. After 120 d, a subsample from each mini silo was taken and the IVDMD was estimated using Ankom Daisy II and F-57 bags (n = 44). Rumen fluid from two cannulated Nellore steers were mixed and placed into fermenter jars containing Ankom buffers (1:4; v/v). After 48-h incubation (39ºC), 40 mL 6 N HCl and 8 g pepsin were added to each jar and incubated for another 24 h. For gas production and pH evaluations, penicillin-type glass vials (n = 40) were filled with McDougall buffer (20 mL), rumen fluid (10 mL) and substrates (200 mg) and incubated in a shaker (39ºC) for 24 h. The cumulative pressure was measured, vials were opened, and terminal pH was measured. Data were analyzed using the MIXED procedure of SAS. The DKB 363 VT PRO3 presented the greatest IVDMD (84.9%, P = 0.02) and the lowest pH (6.60, P = 0.002). The opposite was observed for DKB 390 VT PRO3, which presented the lowest IVDMD (71.1%) and the greatest pH (6.65). The BM 709 PRO2 produced more gas than the others (246.1 mL/g DM), while SYN 555 VIP3 produced less gas than the others (167.7 mL/g DM, P = 0.0005). In conclusion, the material DKB 363 VT PRO3 presented the most interesting characteristics for use as silage for beef cattle in Iturama, Brazil.

scholarly journals PSXIV-13 Effect of phytochemicals on in vitro rumen fermentation and methane production

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 445-446
Author(s):  
Aaron A Molho-Ortiz ◽  
Atmir Romero-Pérez ◽  
Efren Ramírez-Bribiesca ◽  
Claudia Marquez-Mota ◽  
Juan Carlos Ramírez-Orejel ◽  
...  
Keyword(s):  
Essential Oils ◽  
Methane Production ◽  
Dry Matter ◽  
Block Design ◽  
Rumen Fermentation ◽  
Gas Production ◽  
Lag Phase ◽  
Similar Response ◽  
Aqueous Extracts

Abstract The objective of this study was to evaluate the effects of eight phytochemicals from four plant species, in two presentations, essential oils (EO) and aqueous extracts (AE) of garlic (GEO, GAE), cinnamon (CEO, CAE), eucalyptus (EEO, EAE) and rosemary (REO, RAE) on rumen fermentation, using the in vitro gas production technique. The experiment was set up as a completely randomized block design in a 2 × 4 factorial arrangement of treatments. All treatments were incubated with 0.5 g of a basal diet (BD; 50% concentrate, 20% alfalfa and 30% corn silage, dry matter basis). Additionally, BD and BD with 30 ppm of sodium monensin (MON) were used as controls. Phytochemicals were evaluated at a single dose of 900 mg/L of inoculum. In vitro dry matter digestibility (IVDMD), maximum volume of gas (Vmax), gas production rate (S) and lag phase (L) were evaluated. Methane (CH4) was determined indirectly, by fixation of CO2present in gas samples with 1M KOH solution, Non-fixed gas was assumed to be CH4. Methane production was correlated with organic matter fermented in the rumen (mL CH4/g OMFR). Data were analyzed using PROC MIXED of SAS ©. The effects of treatments were tested for the following contrasts: EO Vs AE, W Vs EO, W Vs AE. Some essential oils (GEO, CEO, REO) decreased CH4 production (mL CH4/g OMFR) and IVDMD by 20.4% and 17.8% compared to control treatments (BD and MON) (P &lt; 0.05). Aqueous extracts showed a similar response (P &lt; 0.05) to control treatments. In conclusion the use of essential oils negatively affected rumen fermentation and the production of CH4 in P cinnamon essential oil.

scholarly journals Combining Orchardgrass and Alfalfa: Effects of Forage Ratios on In Vitro Rumen Degradation and Fermentation Characteristics of Silage Compared with Hay

Animals ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 59 ◽  
Author(s):  
Zhulin Xue ◽  
Nan Liu ◽  
Yanlu Wang ◽  
Hongjian Yang ◽  
Yuqi Wei ◽  
...  
Keyword(s):  
Dry Matter ◽  
Protein Production ◽  
Rumen Fluid ◽  
Gas Production ◽  
Neutral Detergent Fiber ◽  
Rumen Microbes ◽  
Rumen Degradation ◽  
Lactating Dairy Cows ◽  
Fermentation Parameters

This study aimed to investigate the effects of different forage ratios of orchardgrass (Dactylis glomerata) and alfalfa (Medicago sativa) on in vitro rumen degradation and fermentation characteristics. Orchardgrass and alfalfa were harvested separately and prepared as hay and silage mixtures at ratios of 100:0, 75:25, 50:50, 25:75, and 0:100 (w/w on a dry matter basis) and anaerobically incubated for 48 h with rumen fluid obtained from lactating dairy cows. Fermented residues and cultured fluids were used to determine nutrient degradability, fermentation parameters, and associative effect indices. Increasing the proportion of alfalfa in hay and silage mixtures quadratically increased in vitro organic matter disappearance (IVOMD, up +5.14%) and marginally decreased in vitro neutral detergent fiber disappearance (NDFD, down −1.79%). Meanwhile, increasing the proportion of alfalfa accelerated the rumen fermentation process (e.g., gas production) and remarkably enhanced the growth of rumen microbes as indicated by microbial protein production (MCP, 13.4% increase). Increments of rumen degradability and methane production were more pronounced in silage mixtures than hay mixtures. In combination, a forage ratio of 50:50 for orchardgrass and alfalfa is recommended for both hay and silage in order to improve the feed use potential in ruminants.

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