Deep Metazoan Phylogeny: The Backbone of the Tree of Life (New Insights from Analyses of Molecules, Morphology, and Theory of Data Analysis).—Edited by J. Wolfgang Wägele and Thomas Bartholomaeus.

AbstractMost phylogenetic analyses assume that a single evolutionary history underlies one gene. However, both biological processes and errors in dataset assembly can violate this assumption causing intragenic conflict. The extent to which this conflict is present in empirical datasets is not well documented. However, if common, it would have far-reaching implications for phylogenetic analyses. Here, we examined several large phylogenomic datasets from diverse taxa using a fast and simple method to identify well supported intragenic conflict. We found conflict to be highly variable between datasets, from 1% to more than 92% of genes investigated. To better characterize patterns of conflict, we analyzed four genes with no obvious data assembly errors in more detail. Analyses on simulated data highlighted that alignment error may be one major source of conflict. Whether as part of data analysis pipelines or in order to explore potential biologically compelling intragenic processes, analyses of within gene signal should become common. The method presented here provides a relatively fast means for identifying conflicts that is agnostic to the generating process. Datasets identified with high intragenic conflict may either have significant errors in dataset assembly or represent conflict generated by biological processes. Conflicts that are the result of error should be identified and discarded or corrected. For those conflicts that are the result of biological processes, these analyses contribute to the growing consensus that, similar to genomes, genes themselves may exhibit multiple conflicting evolutionary histories across the tree of life.

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Microchemical imaging in biomedical research

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100130225 ◽

1992 ◽

Vol 50 (2) ◽

pp. 1118-1119

Author(s):

P. Ingram

Keyword(s):

Data Analysis ◽

Electron Probe ◽

The Other ◽

X Ray ◽

Cell Element ◽

Physiological Information ◽

Functional States ◽

Elemental Maps ◽

Electron Energy Loss ◽

Secondary Ion

It is well established that unique physiological information can be obtained by rapidly freezing cells in various functional states and analyzing the cell element content and distribution by electron probe x-ray microanalysis. (The other techniques of microanalysis that are amenable to imaging, such as electron energy loss spectroscopy, secondary ion mass spectroscopy, particle induced x-ray emission etc., are not addressed in this tutorial.) However, the usual processes of data acquisition are labor intensive and lengthy, requiring that x-ray counts be collected from individually selected regions of each cell in question and that data analysis be performed subsequent to data collection. A judicious combination of quantitative elemental maps and static raster probes adds not only an additional overall perception of what is occurring during a particular biological manipulation or event, but substantially increases data productivity. Recent advances in microcomputer instrumentation and software have made readily feasible the acquisition and processing of digital quantitative x-ray maps of one to several cells.

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Perceptions of a Collaborative Professional Learning Program: Seeing the “Bigger Picture”

Perspectives of the ASHA Special Interest Groups ◽

10.1044/2019_persp-19-00041 ◽

2020 ◽

Vol 5 (1) ◽

pp. 290-303

Author(s):

P. Charlie Buckley ◽

Kimberly A. Murza ◽

Tami Cassel

Keyword(s):

Data Analysis ◽

Professional Learning ◽

Social Communication ◽

Special Educators ◽

Service Providers ◽

Integrative Approach ◽

Theory Approach ◽

Learning Program ◽

Speech Language Pathologists ◽

Related Service

Purpose The purpose of this study was to explore the perceptions of special education practitioners (i.e., speech-language pathologists, special educators, para-educators, and other related service providers) on their role as communication partners after participation in the Social Communication and Engagement Triad (Buckley et al., 2015 ) yearlong professional learning program. Method A qualitative approach using interviews and purposeful sampling was used. A total of 22 participants who completed participation in either Year 1 or Year 2 of the program were interviewed. Participants were speech-language pathologists, special educators, para-educators, and other related service providers. Using a grounded theory approach (Glaser & Strauss, 1967 ) to data analysis, open, axial, and selective coding procedures were followed. Results Three themes emerged from the data analysis and included engagement as the goal, role as a communication partner, and importance of collaboration. Conclusions Findings supported the notion that educators see the value of an integrative approach to service delivery, supporting students' social communication and engagement across the school day but also recognizing the challenges they face in making this a reality.

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Communicative Performance of Adolescents with Severe Speech Impairment

Journal of Speech and Hearing Disorders ◽

10.1044/jshd.5403.403 ◽

1989 ◽

Vol 54 (3) ◽

pp. 403-421 ◽

Cited By ~ 7

Author(s):

Beth M. Dalton ◽

Jan L. Bedrosian

Keyword(s):

Data Analysis ◽

Peer Interactions ◽

Consistent Pattern ◽

Clinical Implications ◽

Speech Impairment ◽

Communication Mode ◽

Mode Function ◽

Communicative Performance ◽

Therapy Room ◽

Communication Board

The communicative performance of 4 preoperational-level adolescents, using limited speech, gestures, and communication board techniques, was examined in a two-part investigation. In Part 1, each subject participated in an academic interaction with a teacher in a therapy room. Data were transcribed and coded for communication mode, function, and role. Two subjects were found to predominantly use the speech mode, while the remaining 2 predominantly used board and one other mode. The majority of productions consisted of responses to requests, and the initiator role was infrequently occupied. These findings were similar to those reported in previous investigations conducted in classroom settings. In Part 2, another examination of the communicative performance of these subjects was conducted in spontaneous interactions involving speaking and nonspeaking peers in a therapy room. Using the same data analysis procedures, gesture and speech modes predominated for 3 of the subjects in the nonspeaking peer interactions. The remaining subject exhibited minimal interaction. No consistent pattern of mode usage was exhibited across the speaking peer interactions. In the nonspeaking peer interactions, requests predominated. In contrast, a variety of communication functions was exhibited in the speaking peer interactions. Both the initiator and the maintainer roles were occupied in the majority of interactions. Pertinent variables and clinical implications are discussed.

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