scholarly journals Conjugated Linoleic Acid and Fish Oil in Laying Hen Diets: Effects on Egg Fatty Acids, Thiobarbituric Acid Reactive Substances, and Tocopherols During Storage

2007 ◽  
Vol 86 (5) ◽  
pp. 953-958 ◽  
Author(s):  
G. Cherian ◽  
M.G. Traber ◽  
M.P. Goeger ◽  
S.W. Leonard
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Fish Oil ◽  
Conjugated Linoleic Acid ◽  
Thiobarbituric Acid ◽  
Laying Hen ◽  
Thiobarbituric Acid Reactive Substances

Conjugated Linoleic Acid Causes a Marked Increase in Liver α-Tocopherol and Liver α-Tocopherol Transfer Protein in C57BL/6 J Mice

2010 ◽  
Vol 80 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Pei-Min Chao ◽  
Wan-Hsuan Chen ◽  
Chun-Huei Liao ◽  
Huey-Mei Shaw
Keyword(s):  
Antioxidant Enzymes ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substances ◽  
Control Groups ◽  
Protein Levels ◽  
Transfer Protein ◽  
And Control

Conjugated linoleic acid (CLA) is a collective term for the positional and geometric isomers of a conjugated diene of linoleic acid (C18:2, n-6). The aims of the present study were to evaluate whether levels of hepatic α-tocopherol, α-tocopherol transfer protein (α-TTP), and antioxidant enzymes in mice were affected by a CLA-supplemented diet. C57BL/6 J mice were divided into the CLA and control groups, which were fed, respectively, a 5 % fat diet with or without 1 g/100 g of CLA (1:1 mixture of cis-9, trans-11 and trans-10, cis-12) for four weeks. α-Tocopherol levels in plasma and liver were significantly higher in the CLA group than in the control group. Liver α-TTP levels were also significantly increased in the CLA group, the α-TTP/β-actin ratio being 2.5-fold higher than that in control mice (p<0.01). Thiobarbituric acid-reactive substances were significantly decreased in the CLA group (p<0.01). There were no significant differences between the two groups in levels of three antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). The accumulation of liver α-tocopherol seen with the CLA diet can be attributed to the antioxidant potential of CLA and the ability of α-TTP induction. The lack of changes in antioxidant enzyme protein levels and the reduced lipid peroxidation in the liver of CLA mice are due to α-tocopherol accumulation.

scholarly journals Influence of Dietary Fish Oil on Conjugated Linoleic Acid and Other Fatty Acids in Milk Fat from Lactating Dairy Cows

2000 ◽  
Vol 83 (11) ◽  
pp. 2620-2628 ◽  
Author(s):  
D.C. Donovan ◽  
D.J. Schingoethe ◽  
R.J. Baer ◽  
J. Ryali ◽  
A.R. Hippen ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Fish Oil ◽  
Dairy Cows ◽  
Conjugated Linoleic Acid ◽  
Milk Fat ◽  
Lactating Dairy Cows ◽  
Dietary Fish

scholarly journals DNA damage by oxidized fatty acids detected by DNA/SPE biosensor

2021 ◽  
Vol 8 (1) ◽  
pp. 45-53
Author(s):  
Ľudmila Sirotová ◽  
Marcela Matulová
Keyword(s):  
Fatty Acids ◽  
Dna Damage ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Thiobarbituric Acid ◽  
Acid Oxidation ◽  
Thiobarbituric Acid Reactive Substances ◽  
Screen Printed Electrode ◽  
Oxidative Products ◽  
Oxidized Fatty Acids

Electrochemical DNA/screen-printed electrode biosensor (DNA/SPE biosensor) was tested for the detection of alterations in DNA formed as a consequence of the reaction between DNA and oxidative products of fatty acids. Interaction of DNA with a mixture of products generated during the oxidation of linoleic and oleic acids manifested DNA damage depending on a tested fatty acid and the presence of hydroperoxides and thiobarbituric acid reactive substances (TBARS) determined after the oxidation of fatty acids. A bigger extent of the DNA damage was registered in the case of the interaction with oxidized linoleic acid with the high content of TBARS. The results achieved suggest the possible application of DNA/SPE biosensor in the detection of an interaction between DNA and products of fatty acid oxidation.

Enrichment of conjugated linoleic acid (CLA) in the eggs of laying hens fed pure isomers of CLA or vaccenic acid

2006 ◽  
pp. 1-5
Author(s):  
Edison Muma ◽  
Samu Palander ◽  
Matti Näsi ◽  
Mikko Griinari
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Egg Quality ◽  
Vaccenic Acid ◽  
Complex Mixture ◽  
Egg Yolk ◽  
Laying Hen ◽  
Total Lipids ◽  
Total Fatty Acids

Conjugated linoleic acid (CLA) is a complex mixture of positional and geometric conjugated isomersof linoleic acid. Dairy products are the major dietary source of CLA and cis-9, trans-11 CLA (9,11CLA) is the predominant isomer resulting from the specific metabolism of feed derived fatty acids inthe rumen. Recent studies suggest endogenous synthesis of CLA by action of Δ-9 desaturase on trans-11 18:1 fatty acid (vaccenic acid, VA) is more important than ruminal production. In view of potentialhuman health benefits, several studies have attempted to enrich eggs with CLA by supplementing thediets of laying hen with mixtures of CLA. This has resulted in marked enrichment but also negativeeffects on egg quality and loss of hatchability of the eggs with concurrent increase in saturated fattyacid content in egg yolk. Such changes are consistent with Δ-9 desaturase inhibition especially associatedwith the presence of the trans-10, cis-12 CLA isomer (10,12 CLA) in the CLA mixtures. It istherefore worthwhile to determine which of the major CLA isomers present in the CLA mixtures, 9,11CLA or 10,12 CLA, is associated with the negative effects on egg quality. This was done by supplementingthe diets of laying hen with relatively pure forms of 9,11 CLA and 10,12 CLA. Furthermore,using relatively pure forms of 9,11 CLA and 10,12 CLA allowed us to determine the transfer efficiencyof 9,11 CLA and 10,12 CLA into egg yolk. In addition, endogenous conversion of VA to 9,11CLA in hens and deposition in egg yolk was examined.Experimental treatments consisted of diets supplemented with 0, 0.5, 1.0 and 1.5 g/d of CLA orVA. CLA isomer content (% of total fatty acids) of the materials was 83 and 7% (9,11 CLA), 9 and85% (10,12 CLA), 35 and 35% (Mix CLA) of 9,11 CLA and 10, 12 CLA, respectively. VA was suppliedin the form of dehydrated hydroxy stearic acid with trans-11 C18:1 content of 28%. The othermajor isomers in this product were cis-11, trans-12 and cis-12 C18:1 (28, 11 and 11%, respectively).There were three hens in each treatment and experimental diets were fed for 21 days. When dietswere supplemented with 9,11 CLA, 10,12 CLA and Mix CLA the transfer efficiencies (percentageof dietary CLA recovered in the egg) of 9,11 CLA and 10,12 CLA in egg yolk total lipids were 21 and<1%, 24 and 12%, 20 and 9%, respectively. Dietary VA was efficiently converted to 9,11 CLA anddeposited in the yolk. The slope of linear regression of VA plus 9,11 CLA versus 9,11 CLA in the eggyolk total lipids represents an estimate of the average conversion over the range of VA intakes studied.In this study 74% of VA available for desaturation was converted to 9,11 CLA. The transfer efficiencyof VA (percentage of dietary VA recovered as VA plus 9,11 CLA in the egg) was 14%. Dietary vaccenicacid in hens is efficiently converted to 9,11 CLA, but the overall efficiency of dietary VA use forCLA enrichment is quite low. Further studies examining the limitations of VA transfer to the liver andavailability for conversion by Δ-9 desaturase should be examined.

Sign in / Sign up

Export Citation Format

Share Document