scholarly journals Mucin Gene Expression and Mucin Content in the Chicken Intestinal Goblet Cells Are Affected by In Ovo Feeding of Carbohydrates

2006 ◽  
Vol 85 (4) ◽  
pp. 669-673 ◽  
Author(s):  
A. Smirnov ◽  
E. Tako ◽  
P.R. Ferket ◽  
Z. Uni
Keyword(s):  
Gene Expression ◽  
Goblet Cells ◽  
In Ovo ◽  
Mucin Gene ◽  
In Ovo Feeding

scholarly journals A50 MODULATION OF GOBLET CELL ACTIVITY DURING GIARDIA DUODENALIS INFECTION: A ROLE FOR PAR2

2021 ◽  
Vol 4 (Supplement_1) ◽  
pp. 6-7
Author(s):  
E Fekete ◽  
C B Amat ◽  
T Allain ◽  
M Hollenberg ◽  
K Mihara ◽  
...  
Keyword(s):  
Gene Expression ◽  
Goblet Cell ◽  
Cysteine Protease ◽  
Protease Activity ◽  
Calcium Release ◽  
Cell Activity ◽  
Goblet Cells ◽  
Giardia Infection ◽  
Mucus Production ◽  
Mucin Gene

Abstract Background Giardia duodenalis has been shown to alter the structure of the intestinal mucus layers during infection via obscure mechanisms. We hypothesize that goblet cell activity may be disrupted in part due to proteolytic activation of protease-activated receptor 2 (PAR2) by Giardia proteases, resulting in disruption of mucus production and secretion by intestinal goblet cells. Aims Characterize alterations in goblet cell activity during Giardia infection, focusing on the roles of Giardia protease activity and PAR2. Methods Chinese hamster ovary cells transfected with nano-luciferase tagged PAR2 were incubated with Giardia NF or GSM trophozoites. Cleavage within the activation domain results in release of enzymes into the supernatant. Luminescence in the supernatant was measured as an indication of PAR cleavage by Giardia. LS174T, a human colonic mucus-producing cell line, was infected with Giardia trophozoites (isolates NF, WB, S2, and GSM). Prior to infection, trophozoites were treated with E64, a broad-spectrum cysteine protease inhibitor, and LS174T were treated with a PAR2 antagonist, a calcium chelator, or an ERK1/2 inhibitor. Quantitative PCR (qPCR) was performed for the MUC2 mucin gene. Wild-type (WT) and PAR2 knockout (KO) mice were infected with Giardia. Colonic mucus was stained using fluorescein-coupled wheat-germ agglutinin (WGA), and qPCR was performed for Muc2 and Muc5ac. Results Giardia trophozoites cleaved PAR2 within the N-terminal activation domain in a cysteine protease-dependent manner. Cleavage was isolate dependent, with isolates that show higher protease activity cleaving at a higher rate. High protease activity Giardia isolates increased MUC2 gene expression in LS714T. This increase was attenuated by inhibition of Giardia cysteine protease activity, and by antagonism of PAR2, inhibition of calcium release, or inhibition of ERK1/2 activity in LS174T cells. Both Muc2 and Muc5ac expression were upregulated in the colons of WT mice in response to Giardia infection, while in the jejunum Muc2 expression decreased and Muc5ac expression increased. In KO, no changes in gene expression were seen in the colon in response to Giardia infection, while in the jejunum, Muc2 expression was unchanged and Muc5ac expression decreased. Both WT infected and KO noninfected mice showed thinning of the colonic mucus layer compared to WT controls. There was some recovery in thickness in KO infected mice. Conclusions PAR2 plays a significant role in the regulation of mucin gene expression in mice and in a human colonic cell line. Results suggest that Giardia cysteine proteases cleave and activate PAR2, leading to calcium release and activation of the MAPK pathway in goblet cells, ultimately leading to altered mucin gene expression. Findings identify a novel regulatory pathway for mucus production by intestinal goblet cells. Funding Agencies CAG, CCC

TEMPORAL ANALYSIS OF GOBLET CELLS AND MUCIN GENE EXPRESSION IN MURINE MODELS OF ALLERGIC ASTHMA

2003 ◽  
Vol 29 (8) ◽  
pp. 549-565 ◽  
Author(s):  
Shahriar Shahzeidi ◽  
Pawandeep K. Aujla ◽  
Tracey J. Nickola ◽  
Yajun Chen ◽  
M. Zuhdi Alimam ◽  
...  
Keyword(s):  
Gene Expression ◽  
Allergic Asthma ◽  
Goblet Cells ◽  
Temporal Analysis ◽  
Murine Models ◽  
Mucin Gene

scholarly journals Differential expression of growth and immunity related genes influenced by in ovo supplementation of amino acids in broiler chickens

2014 ◽  
Vol 59 (No. 9) ◽  
pp. 399-408 ◽  
Author(s):  
S.K. Bhanja ◽  
M. Sudhagar ◽  
A. Goel ◽  
N. Pandey ◽  
M. Mehra ◽  
...  
Keyword(s):  
Gene Expression ◽  
Amino Acids ◽  
Growth Hormone ◽  
Broiler Chickens ◽  
In Ovo ◽  
Immune Genes ◽  
Mucin Gene ◽  
Igf I ◽  
Insulin Like Growth Factors

The present study was aimed at investigating the role of in ovo administered amino acids: lysine, arginine, threonine or methionine plus cysteine (Met+Cys) in 14-day embryos on expression profile of growth (chicken growth hormone (cGH), insulin like growth factors (IGF) I and II, and mucin) and immunity related genes (IL-2, IL-4, IL-6, IL-12, TNF-α, and IFN-γ). On incubation day (ID) 18, higher (P < 0.01) cGH and mucin gene expression was observed in lysine, threonine, arginine or Met+Cys injected embryos, while IGF-II expression was higher in threonine, arginine or Met+Cys injected embryos on ID 20. Expression of growth genes was down regulated (P < 0.01) on day of hatch in most of the amino acids injected chicks. On day 7 post-hatch (PH), threonine or arginine exhibited higher expression of cGH, IGF-I, and IGF-II but higher mucin gene expression only on day 14 PH. Threonine or Met+Cys injected birds had higher expression of IL-6 and TNF-α, while arginine injected birds had higher TNF-α expression. Lysine, threonine or Met+Cys injected birds had higher IL-2, but lower of IL-12 and IFN-γ gene expression. It is concluded that arginine and threonine enhanced the expression of growth related genes, while threonine and Met+Cys modulated expression of immune genes in broiler chickens.  

Expression of Mucin Genes in Chronic Ethmoiditis

2000 ◽  
Vol 14 (3) ◽  
pp. 163-170 ◽  
Author(s):  
Hak Hyun Jung ◽  
Je Hyuck Lee ◽  
Young Tae Kim ◽  
Sang Duck Lee ◽  
Jae Hoon Park
Keyword(s):  
Gene Expression ◽  
Electron Microscope ◽  
Chronic Inflammation ◽  
Goblet Cells ◽  
Rt Pcr ◽  
Electron Microscopic ◽  
Mucin Gene ◽  
Mucin Genes ◽  
Microscopic Studies ◽  
Muc Genes

We have investigated the profiles of MUC genes expressed in chronic ethmoiditis mucosa and normal ethmoid mucosa using RT-PCR, and the morphology of chronic ethmoiditis by a combination of light and electron microscope was observed. In the light- and electron-microscopic studies, chronic ethmoiditis mucosa revealed increased numbers of goblet cells with higher production of mucus in comparison to normal ethmoid mucosa. RT-PCR of cDNAs from three normal ethmoid mucosa revealed the same pattern of mucin gene expression, such as MUC5AC and MUC8. However, RT-PCR of cDNAs from eight chronic ethmoiditis mucosa showed the expression of two MUC1, six MUC4, eight MUC5AC, five MUC5B, seven MUC7, and eight MUC8. MUC2 and MUC6 were not detected. These results suggest that MUC4, MUC5AC, MUC5B, MUC7, and MUC8 are major mucins in the ethmoid mucosa and are up-regulated by chronic inflammation.

Mucin gene expression in OME: cellular localization

1998 ◽  
Vol 23 (3) ◽  
pp. 281-282
Author(s):  
Hutton ◽  
Guo ◽  
Birchall ◽  
Pearson
Keyword(s):  
Gene Expression ◽  
Cellular Localization ◽  
Mucin Gene

Mucin gene expression and lymph node metastasis in early gastric cancer

1998 ◽  
Vol 114 ◽  
pp. A623
Author(s):  
HR Kim ◽  
SJ Pang ◽  
HY Jung ◽  
WS Hong ◽  
YI Min ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Early Gastric Cancer ◽  
Node Metastasis ◽  
Mucin Gene
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