scholarly journals Chicken embryo extract mitigates growth and morphological changes in a spontaneously immortalized chicken embryo fibroblast cell line

2005 ◽  
Vol 84 (9) ◽  
pp. 1423-1431 ◽  
Author(s):  
S.A. Christman ◽  
B.W. Kong ◽  
M.M. Landry ◽  
D.N. Foster
Keyword(s):  
Cell Line ◽  
Chicken Embryo ◽  
Morphological Changes ◽  
Chicken Embryo Fibroblast ◽  
Fibroblast Cell ◽  
Chicken Embryo Fibroblast Cell ◽  
Fibroblast Cell Line ◽  
Embryo Extract

scholarly journals The jaagsiekte sheep retrovirus envelope gene induces transformation of the avian fibroblast cell line DF-1 but does not require a conserved SH2 binding domain

2002 ◽  
Vol 83 (11) ◽  
pp. 2733-2742 ◽  
Author(s):  
Thomas E. Allen ◽  
Kate J. Sherrill ◽  
Sara M. Crispell ◽  
Matthew R. Perrott ◽  
Jonathan O. Carlson ◽  
...  
Keyword(s):  
Cell Line ◽  
Fluorescent Protein ◽  
Transmembrane Domain ◽  
Mutational Analysis ◽  
Morphological Changes ◽  
Fibroblast Cell ◽  
Envelope Gene ◽  
Avian Embryo ◽  
Fibroblast Cell Line ◽  
Jaagsiekte Sheep Retrovirus

Ovine pulmonary adenocarcinoma, caused by jaagsiekte sheep retrovirus (JSRV), is a naturally occurring retrovirus-induced pulmonary neoplasm of sheep. We report here that expression of the JSRV env gene is sufficient to transform an avian embryo fibroblast cell line, DF-1. DF-1 cells transfected with an avian sarcoma–leukaemia retroviral expression vector containing the JSRV env gene [pRCASBP(A)-J:env] exhibited changes consistent with transformation, including contraction and rounding of cells with formation of dense foci. Transfection with a reporter construct expressing the green fluorescent protein did not induce morphological changes in DF-1 cells, eliminating the possibility that the vector, the transfection protocol or culturing techniques were responsible for the transformed phenotype. When pRCASBP(A)-J:env-transfected cells were inoculated into nude mice, tumours formed, verifying that the DF-1 cells were tumorigenic. Analysis of the JSRV env gene revealed a conserved tyrosine (597) and methionine (600) residue in the cytoplasmic tail within the transmembrane domain of the envelope, which creates a known binding site of SH2 domains in the p85 subunit of phosphatidylinositol 3-kinase. However, when this tyrosine residue was mutated to serine or alanine, transformation was not affected. Furthermore, mutation of the methionine residue to valine or leucine also failed to eliminate JSRV env-mediated transformation. These results are in contrast to mutational analysis performed in JSRV env-transformed murine NIH-3T3 cells in which both the tyrosine and methionine residues are necessary for transformation. These findings suggest that more than one mechanism may be involved in JSRV env-mediated transformation.

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