scholarly journals Glutathione and respiratory chain complex activity in duodenal mitochondria of broilers with low and high feed efficiency

2005 ◽  
Vol 84 (5) ◽  
pp. 782-788 ◽  
Author(s):  
C. Ojano-Dirain ◽  
M. Iqbal ◽  
T. Wing ◽  
M. Cooper ◽  
W. Bottje
Keyword(s):  
Respiratory Chain ◽  
Feed Efficiency ◽  
Chain Complex ◽  
Respiratory Chain Complex ◽  
Complex Activity ◽  
High Feed

Relationships between the activity of respiratory-chain complexes in pre- (biopsy) or post-slaughter muscle samples and feed efficiency in random-bred Ghezel lambs

2017 ◽  
Vol 57 (8) ◽  
pp. 1674
Author(s):  
M. J. Zamiri ◽  
R. Mehrabi ◽  
G. R. Kavoosi ◽  
H. Rajaei Sharifabadi
Keyword(s):  
Respiratory Chain ◽  
Feed Intake ◽  
Enzyme Activities ◽  
Feed Efficiency ◽  
Average Daily Gain ◽  
Chain Complex ◽  
Respiratory Chain Complex ◽  
Respiratory Chain Complexes ◽  
Chain Complexes ◽  
Daily Gain

The present study was conducted to determine the relationship between the activity of mitochondrial respiratory chain complexes in pre- and post-slaughter muscle samples and residual feed intake (RFI) in Ghezel male lambs born as a result of random mating. The study was based on the hypothesis that random-bred lambs with lower feed (or higher) RFI have lower (or higher) respiratory chain-complex activity in muscle samples. Lambs (n = 30) were fed a diet consisting of 70% concentrate and 30% alfalfa hay during a 70-day period. Individual feed intake and average daily gain were recorded to calculate the RFI, feed-conversion ratio (FCR) and adjusted FCR (aFCR). On the basis of these calculations, the lambs were classified into low and high groups for RFI, with FCR and aFCR (n = 22) being one standard deviation above or below the means; this was corroborated by Student’s t-test (P < 0.01). At the end of the experiment, a 10-g biopsy sample was taken from the posterior side of the left femoral biceps. After 24 h, the lambs were slaughtered, and a sample from the posterior side of the right femoral biceps was dissected for determination of mitochondrial protein and respiratory chain-complex activities (Complexes I–V). The RFI was not correlated with the metabolic bodyweight and average daily gain, but was positively correlated (r = 0.56) with the average daily feed intake (P < 0.01); mean daily feed intake in the low-RFI group was 200 g less than that in the high-RFI group. The FCR and aFCR were not significantly (P > 0.05) correlated with average daily feed intake (r = 0.39 and r = 0.36 respectively), but showed a negative correlation (P < 0.01) with average daily gain (r = –0.73 and r = –0.76 respectively). Although very high negative correlations were recorded between the activities of all five respiratory-chain complexes and RFI in muscle samples obtained before (–0.91 to –0.97) and after (–0.92 to –0.97) slaughter, Complexes I and V showed small negative correlations (–0.40) with FCR or aFCR (P < 0.05). Enzyme activities of the respiratory-chain Complexes I, III and V were not significantly different between the pre- and post-slaughter biopsy samples; however, the enzyme activities of respiratory-chain Complexes II and IV were slightly higher in post-slaughter samples (P < 0.01). These results suggested that it may be possible to use the enzymatic activity of respiratory-chain complexes in muscle biopsy samples for screening of lambs for RFI, providing a useful procedure for genetic selection of lambs for this component of feed efficiency. These encouraging results need to be verified in further experiments using other sheep breeds and a larger number of lambs.

scholarly journals Antimicrobial Susceptibility and Antibacterial Mechanism of Limonene against Listeria monocytogenes

Molecules ◽  
2019 ◽  
Vol 25 (1) ◽  
pp. 33 ◽  
Author(s):  
Yingjie Han ◽  
Zhichang Sun ◽  
Wenxue Chen
Keyword(s):  
Listeria Monocytogenes ◽  
Cell Membrane ◽  
Respiratory Chain ◽  
Antimicrobial Susceptibility ◽  
Chain Complex ◽  
Cell Membrane Permeability ◽  
Wall Structure ◽  
Antibacterial Mechanism ◽  
Respiratory Chain Complex ◽  
Complex Activity

Limonene is a monoterpenoid compound, which is founded in a lot of plants’ essential oils with good antibacterial activity against food-borne pathogens, but it has an ambiguous antimicrobial susceptibility and mechanism against Listeria monocytogenes (L. monocytogenes). In this study, the antimicrobial susceptibility of Limonene to L. monocytogenes was studied, and some new sights regarding its antibacterial mechanism were further explored. Scanning electron microscopy (SEM) verified that limonene caused the destruction of the cell integrity and wall structure of L. monocytogenes. The increase in conductivity and the leakage of intracellular biomacromolecules (nucleic acids and proteins) confirmed that limonene had an obvious effect on cell membrane permeability. The results of Propidium Iodide (PI) fluorescence staining were consistent with the results of the conductivity measurements. This indicated that limonene treatment caused damage to the L. monocytogenes cell membrane. Furthermore, the decrease in ATP content, ATPase (Na+K+-ATPase, Ca2+-ATPase) activity and respiratory chain complex activity indicated that limonene could hinder ATP synthesis by inhibiting the activity of the respiratory complex and ATPase. Finally, differential expression of proteins in the respiratory chain confirmed that limonene affected respiration and energy metabolism by inhibiting the function of the respiratory chain complex.

Exercise can induce temporary mitochondrial and contractile dysfunction linked to impaired respiratory chain complex activity

Metabolism ◽  
2012 ◽  
Vol 61 (1) ◽  
pp. 117-126 ◽  
Author(s):  
Maria Schoepe ◽  
Andrea Schrepper ◽  
Michael Schwarzer ◽  
Moritz Osterholt ◽  
Torsten Doenst
Keyword(s):  
Respiratory Chain ◽  
Chain Complex ◽  
Contractile Dysfunction ◽  
Respiratory Chain Complex ◽  
Complex Activity

Biochemical Assays of Respiratory Chain Complex Activity

2007 ◽  
pp. 93-119 ◽  
Author(s):  
Denise M. Kirby ◽  
David R. Thorburn ◽  
Douglass M. Turnbull ◽  
Robert W. Taylor
Keyword(s):  
Respiratory Chain ◽  
Chain Complex ◽  
Respiratory Chain Complex ◽  
Complex Activity ◽  
Biochemical Assays
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