scholarly journals Cell-mediated and humoral immune responses in broiler chickens maintained on diets containing different levels of vitamin A

1997 ◽  
Vol 76 (10) ◽  
pp. 1368-1378 ◽  
Author(s):  
M. Lessard ◽  
D. Hutchings ◽  
N.A. Cave
2013 ◽  
Vol 56 (1) ◽  
pp. 842-850 ◽  
Author(s):  
M. M. Saei ◽  
A. A. Sadeghi ◽  
H. Ahmadvand

Abstract. A study was conducted to investigate the capability of Myrtus communis essential oil (MCE) in counteracting the deleterious effects of aflatoxin B1 (AFB1) on growth performance, serum biochemistry and humoral immune responses in broiler chickens. In a completely randomized design, 300 day-old male chicks were assigned to four treatments with five replicates of 15 birds for 42 days. Chickens, up to day 7 of age, were fed the same diet and then, they were fed the experimental diets. The dietary treatments were 1) the negative control (no dietary aflatoxin or MCE), 2) the positive control (diet containing AFB1 at 0.5 mg/kg, without MCE), 3) diet containing AFB1 at 0.5 mg/kg plus 500 mg/kg MCE, and 4) basal diet containing 500 mg/kg MCE, without AFB1. Growth performance was measured from day 7 to 42. Serum biochemical parameters, organ weights on day 42 and the antibody titers against Newcastle and influenza viruses on day 28 of age were determined. Addition of aflatoxin to diet decreased (P<0.05) the weight gain and feed intake and MCE supplementation diminished (P<0.05) the inhibitory effects of AFB1 on the growth performance. Addition of AFB1 to diet of chicks increased the serum activities of aspartate aminotransferase (AST), alkaline aminotransferase (ALT), alkaline phosphatase (ALP), and decreased the antibody titers against Newcastle and influenza viruses. Addition of MCE to diet alleviated the negative effects of AFB1 on these parameters (P<0.05). In conclusion, our results showed that addition of MCE may reduce the adverse effects of AFB1 on broiler chickens.


2020 ◽  
Vol 51 (6) ◽  
pp. 1716-1725
Author(s):  
Jawad & ALwan

Forty broiler chickens, One - day old were randomly divided into four equal groups:  1st group was immunized with 0.5 ml of whole sonicated salmonella antigens (WSSAgs), protein concentration 1.89 mg/ml. Two dose  two weeks intervals, S/C at 7 days old  and  the  chicks  fed   contaminated diet with  mycotoxins for 7 week,   2nd group was immunized with WSSAgs only and treated  as  1st group,  3rd group fed diet contaminated with  mycotoxins  and 4th group was fed  normal diets and served as control negative group, At 30 days, skin test, phagocytic index and serum levels of antibody titers were done, then 1st ,2nd and 3rd groups were inoculated with  high dose of  virulent S.typhimurium , (1ml containing   1  10 12  CFU/ml ), I/V, and  4th group was inoculated I/V,1ml  sterile normal saline and served as control negative group , all chicks were sacrificed at  3 weeks post infection, it was recorded that mycotoxin suppress the  cellular and  humoral immune responses , phagocytic activity ,in addition to high mortality rate were found in  chicks fed contaminated diet with  and without immunization.


Author(s):  
Katayoon Nofouzi ◽  
Sorayya Mirzazadeh ◽  
Monireh Khordadmehr ◽  
Mohammad Sadegh Madadi ◽  
Soheila Amininia ◽  
...  

Background and Objectives: Tsukamurella species are Gram-positive rods that exist in a broad range of environments. In this study, the efficacy of heat-killed Tsukamurella inchonensis on growth performance, intestinal morphology, and humoral immune responses of broiler chicken was evaluated. Materials and Methods: Ross broiler chicks in the cage were randomly allocated to five groups. Trail diets were prepared by adding 106 cells per bird of heat-killed T. inchonensis into the basal trading diet for group 1 continuously dosed for 24 h from day 1 to day 13, and for group 2, 24 h on days 1 to 5; 8; 9, 12 and 13. Group 3 was received 10 6 bacteria as a subcutaneous injection on days 1, 6, and 12. Groups 4 and 5 were not received T. inchonensis during the experiment period. Results: Feed intake (FI) and feed conversion ratio (FCR) were not altered by different delivery methods of T. inchonensis supplementation. The pulsed dosed in feed tended to provide higher body weight gain (BWG) than the negative control groups. T. inchonensis treatments, never less of the ways of delivery, boosted (P<0.05) the antibody titers to Newcastle disease virus (NDV), and avian influenza (AI) (H9N2) virus, especially when broiler chickens treated with pulse dosed in the feed. The most significant intestinal development (p<0.05) was observed between groups 1 and 2. There were no significant differences in the thymus, liver, and bursa of Fabricius relative weight. Still, there were significant increases in the relative weight of spleen on day 14 in vaccinated chickens treated with T. inchonensis pulse dosed. Conclusion: It seems that the supplementation of T. inchonensis in the broiler diet can improve intestinal morphology and humoral immune response, which was represented by increased antibody response to NDV, and AI vaccines significantly, but it cannot affect FI and FCR.


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