scholarly journals Heat shock protein 101 (HSP101) promotes flowering under nonstress conditions

2021 ◽  
Author(s):  
Feng Qin ◽  
Buzhu Yu ◽  
Weiqi Li
Keyword(s):  
Arabidopsis Thaliana ◽  
Gene Regulation ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Regulation Mechanism ◽  
Multiple Gene ◽  
Late Flowering ◽  
Expression Of Genes ◽  
Shock Proteins

Abstract Heat shock proteins (HSPs) are stress-responsive proteins that are conserved across all organisms. Heat shock protein 101 (HSP101) has an important role in thermotolerance owing to its chaperone activity. However, if and how it functions in development under nonstress conditions is not yet known. By using physiological, molecular, and genetic methods, we investigated the role of HSP101 in the control of flowering in Arabidopsis (Arabidopsis thaliana (L.) Heynh.) under nonstress conditions. Knockout and overexpression of HSP101 cause late and early flowering, respectively. Late flowering can be restored by rescue of HSP101. HSP101 regulates the expression of genes involved in the six known flowering pathways; the most negatively regulated genes are FLOWERING LOCUS C (FLC) and SHORT VEGETATIVE PHASE (SVP); downstream integrators of the flowering pathways are positively regulated. The late-flowering phenotype of loss-of-HSP101 mutants is suppressed by both the mutations of FLC and SVP. The responses of flowering time to exogenous signals do not change in HSP101 mutants. HSP101 is also found in nonspecific regions according to subcellular localization. We found that HSP101 promotes flowering under nonstress conditions and that this promotion depends on FLC and SVP. Our data suggest that this promotion could occur through a multiple gene regulation mechanism.

scholarly journals CAESAREAN SECTION AND THE RISK OF POSTPARTAL DEPRESSION: IS THERE A POSSIBLE ROLE OF HEAT SHOCK PROTEINS?

2020 ◽  
Author(s):  
Petra ◽  
Krunoslav Šporčić ◽  
Marko Ćaćić ◽  
Krešimir Reiner ◽  
Matko Pavlović ◽  
...  
Keyword(s):  
Heat Shock ◽  
Caesarean Section ◽  
Heat Shock Proteins ◽  
Surgical Stress ◽  
Meta Analysis ◽  
Randomised Control Trial ◽  
Expression Of Genes ◽  
The One ◽  
Shock Proteins

Data on the potential connection between surgical stress during caesarean section and the role of heat shock proteins in development of postpartal depression is lacking in the literature. This is a narrative review with a goal to establish the potential role of heat shock proteins during caesarean section and development of postpartal depression. Systemic hyperinflammatory state, suchas the one that occurs during surgery, may trigger protective cell reaction, which is usually called the heat shock response. Results of several researches bring strong evidence of correlation between expression of genes coding for family of heat shock proteins with the onset of depressive symptoms. Also, a recent meta-analysis established caesarean section as a risk factor for development of postpartal depression. It is obvious that heat shock proteins play a certain role in development of psychiatric disorders. However, a role of heat shock proteins in development of postpartal depression remains open for debate. We emphasise the need for a randomised control trial which would enable an answer to the mentioned issue.

scholarly journals OsMSR3, a Small Heat Shock Protein, Confers Enhanced Tolerance to Copper Stress in Arabidopsis thaliana

2019 ◽  
Vol 20 (23) ◽  
pp. 6096 ◽  
Author(s):  
Yanchun Cui ◽  
Manling Wang ◽  
Xuming Yin ◽  
Guoyun Xu ◽  
Shufeng Song ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Growth And Development ◽  
Oryza Sativa L ◽  
Metal Resistance ◽  
Copper Stress ◽  
Cu Stress ◽  
Responsive Gene

Copper is a mineral element essential for the normal growth and development of plants; however, excessive levels can severely affect plant growth and development. Oryza sativa L. multiple stress-responsive gene 3 (OsMSR3) is a small, low-molecular-weight heat shock protein (HSP) gene. A previous study has shown that OsMSR3 expression improves the tolerance of Arabidopsis to cadmium stress. However, the role of OsMSR3 in the Cu stress response of plants remains unclear, and, thus, this study aimed to elucidate this phenomenon in Arabidopsis thaliana, to further understand the role of small HSPs (sHSPs) in heavy metal resistance in plants. Under Cu stress, transgenic A. thaliana expressing OsMSR3 showed higher tolerance to Cu, longer roots, higher survival rates, biomass, and relative water content, and accumulated more Cu, abscisic acid (ABA), hydrogen peroxide, chlorophyll, carotenoid, superoxide dismutase, and peroxidase than wild-type plants did. Moreover, OsMSR3 expression in A. thaliana increased the expression of antioxidant-related and ABA-responsive genes. Collectively, our findings suggest that OsMSR3 played an important role in regulating Cu tolerance in plants and improved their tolerance to Cu stress through enhanced activation of antioxidative defense mechanisms and positive regulation of ABA-responsive gene expression.

scholarly journals THE ROLE OF THE HEAT SHOCK PROTEIN 70 IN THE PATHOGENESIS OF CARDIOVASCULAR PATHOLOGY

2019 ◽  
Vol 21 (2) ◽  
pp. 201-208
Author(s):  
O. A. Ponasenko ◽  
L. V. Gankovskaya ◽  
O. A. Svitich
Keyword(s):  
Innate Immunity ◽  
Cardiovascular Diseases ◽  
Heat Shock ◽  
Heat Shock Proteins ◽  
Heat Shock Protein ◽  
Arterial Hypertension ◽  
Heat Shock Protein 70 ◽  
Cardiovascular Pathology ◽  
Shock Proteins

The problem of studying cardiovascular diseases (CVD) for a long time remains extremely important, and, therefore, there are many works that offer new ways to diagnose and treat this group of diseases. Great opportunities are provided by the study of molecular interactions for a more accurate understanding of the pathogenesis of cardiovascular pathology. Many studies have recently been devoted to finding potential markers of CVD risk with the aim of more accurate and early diagnosis. In this review we analyze the latest literature data dedicated to the role of heat shock protein 70 (HSP70) in cardiovascular pathology. HSP70 take part in such processes as arterial hypertension, coronary heart disease, and atherosclerosis. In atherogenesis, serum heat shock proteins 70 play a major role. It has been proven that in patients with a high concentration of heat shock protein molecules circulating in the blood, increased values of the carotid intima-media complex were observed. The important role of antibodies to circulating HSP70 is noted. Found an association of high levels of these antibodies with atherosclerosis in patients with arterial hypertension in history, with myocardial infarction. Low levels of anti-HSP70 antibodies are observed in patients with acute coronary syndrome. This proves the complexity of the mechanism and the dual role of antibodies against serum heat shock proteins 70. Thus, antibodies against heat shock proteins 70 can be assessed as a protective marker, and as a predictor, which requires further study, and the HSP70 molecules themselves can somehow to participate in the development of cardiovascular pathologies. Much attention is paid to the role of the inflammatory process and the mechanisms of innate immunity in CVD. As it is currently believed that Danger-associated molecular patterns (DAMPs) are involved in the pathogenesis of these pathologies in the context of a “hazard/damage” model. According to this model, the triggering factor is stress, leading to the release of DAMPs and their binding to innate immunity receptors - Toll-like receptors (TLRs). Activation of TLRs triggers the signaling cascade in the cell leading to the synthesis of pro-inflammatory cytokines. This contributes to the development of inflammation, which can provoke the emergence of new pathological processes in the body and worsen the course of existing diseases. The identification of new potential markers and knowledge of the molecular mechanisms of the pathogenesis of CVD can play an important role in the development of a new individual approach to the prevention of cardiovascular diseases.

scholarly journals CLINICAL AND THERAPEUTIC SIGNIFICANCE OF HEAT SHOCK PROTEINS

2021 ◽  
Vol 9 (02) ◽  
pp. 961-964
Author(s):  
Sreeja Nannapaneni ◽  
◽  
Gnana Sri Deepika Vusthepalli ◽  
Pavan Santhosh Guptha Vusthepalli ◽  
Kusuma Naredla ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Heat Shock Protein ◽  
Cell Biology ◽  
Experimental Investigations ◽  
Immunological Responses ◽  
Immune Mediated ◽  
Clinical Biomarker ◽  
Shock Proteins

Multiple experimental investigations have been successful in suggesting the role of heat shock protein as a clinical biomarker and therapeutic target in several diseases. All living cells, from the simplest prokaryote to the most complex multicellular organism, contain heat shock proteins-molecular chaperones that are responsible for management of unfolded polypeptides within the cell. In view of the fundamental role of heat shock proteins in maintenance of protein homeostasis, it seems likely that malfunctions associated with members of heat shock protein families would have pathological effects. Such effects might be minimal under normal physiological conditions, but could be exacerbated at times. This review provides an overview of the cell biology and immunology of heat shock proteins focusing predominantly on immunological responses to heat shock proteins in a range of immune-mediated diseases and in infectious diseases.

scholarly journals A new antisarcoma strategy: multisubtype heat shock protein/peptide immunotherapy combined with PD-L1 immunological checkpoint inhibitors

2021 ◽  
Author(s):  
H. Li ◽  
X. Sui ◽  
Z. Wang ◽  
H. Fu ◽  
Z. Wang ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Lung Metastasis ◽  
Checkpoint Inhibitors ◽  
Combined Therapy ◽  
Peptide Vaccine ◽  
Tumor Environment ◽  
Immune Check Point Inhibitor ◽  
Shock Proteins ◽  
Sarcoma Treatment

AbstractOsteosarcoma, a common malignant tumor in orthopedics, often has a very poor prognosis after lung metastasis. Immunotherapy has not achieved much progress in the treatment because of the characteristics of solid tumors and immune environment of osteosarcoma. The tumor environment is rather essential for sarcoma treatment. Our previous study demonstrated that heat shock proteins could be used as antitumor vaccines by carrying tumor antigen peptides, and we hypothesize that an anti-osteosarcoma effect may be increased with an immune check point inhibitor (PD-L1 inhibitor) as a combination treatment strategy. The present study prepared a multisubtype mixed heat shock protein osteosarcoma vaccine (mHSP/peptide vaccine) and concluded that the mHSP/peptide vaccine was more effective than a single subtype heat shock protein, like Grp94. Therefore, we used the mHSP/peptide vaccine in combination with a PD-L1 inhibitor to treat osteosarcoma, and the deterioration of osteosarcoma was effectively hampered. The mechanism of combined therapy was investigated, and AKT expression participates with sarcoma lung metastasis. This study proposed an antisarcoma strategy via stimulation of the immune system as a further alternative approach for sarcoma treatment and elucidated the mechanism of combined therapy.

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