scholarly journals Mapping and engineering of auxin-induced plasma membrane dissociation in BRX family proteins

2021 ◽  
Author(s):  
Samuel W H Koh ◽  
Petra Marhava ◽  
Surbhi Rana ◽  
Alina Graf ◽  
Bernard Moret ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Biological Activity ◽  
Plasma Membrane ◽  
Vascular Plant ◽  
Amino Acid Substitutions ◽  
Long Distance ◽  
Long Distance Transport ◽  
Complex Process ◽  
Necessary And Sufficient ◽  
Distance Transport

Abstract Angiosperms have evolved the phloem for the long-distance transport of metabolites. The complex process of phloem development involves genes that only occur in vascular plant lineages. For example, in Arabidopsis thaliana, the BREVIS RADIX (BRX) gene is required for continuous root protophloem differentiation, together with PROTEIN KINASE ASSOCIATED WITH BRX (PAX). BRX and its BRX-LIKE (BRXL) homologs are composed of four highly conserved domains including the signature tandem BRX domains that are separated by variable spacers. Nevertheless, BRX family proteins have functionally diverged. For instance, BRXL2 can only partially replace BRX in the root protophloem. This divergence is reflected in physiologically relevant differences in protein behavior, such as auxin-induced plasma membrane dissociation of BRX, which is not observed for BRXL2. Here we dissected the differential functions of BRX family proteins using a set of amino acid substitutions and domain swaps. Our data suggest that the plasma membrane-associated tandem BRX domains are both necessary and sufficient to convey the biological outputs of BRX function and therefore constitute an important regulatory entity. Moreover, PAX target phosphosites in the linker between the two BRX domains mediate the auxin-induced plasma membrane dissociation. Engineering these sites into BRXL2 renders this modified protein auxin-responsive and thereby increases its biological activity in the root protophloem context.

scholarly journals Cut and paste – Efficient cotyledon micrografting for Arabidopsis thaliana seedlings

2019 ◽  
Author(s):  
Kai Bartusch ◽  
Jana Trenner ◽  
Marcel Quint
Keyword(s):  
Arabidopsis Thaliana ◽  
Success Rates ◽  
Long Distance ◽  
Long Distance Transport ◽  
Light Intensities ◽  
Whole Plant ◽  
Plant Level ◽  
Distance Transport ◽  
Plant Seedlings

AbstractCotyledon micrografting represents a very useful tool for studying the central role of cotyledons during early plant development, especially their interplay with other plant organs with regard to long distance transport. While hypocotyl grafting methods are established, cotyledon grafting is still inefficient. By optimizing cotyledon micrografting, we aim for higher success rates and increased throughput in the model species Arabidopsis thaliana. We established a cut and paste cotyledon surgery procedure on a flat solid but moist surface which improved handling of small plant seedlings. Applying a specific cutting and joining pattern throughput was increased up to 40 seedlings per hour. The combination of short day conditions and low light intensities for germination and long day plus high light intensities and vertical plate positioning after grafting significantly increased ‘ligation’ efficiency. Together, we achieved up to 46 % grafting success in A. thaliana. Reconnection of vasculature was shown by successful transport of a vasculature-specific dye across the grafting site. On a whole plant level, plants with grafted cotyledons match plants with intact cotyledons in biomass production and rosette development. This cut and paste cotyledon-to-petiole grafting protocol simplifies the handling of plant seedlings in surgery, increases the number of grafted plants per hour and produces higher success rates for A. thaliana seedlings. The developed cotyledon micrografting method is also suitable for other plant species of comparable size.

scholarly journals AtOPT6 Protein Functions in Long-Distance Transport of Glutathione in Arabidopsis thaliana

2018 ◽  
Author(s):  
Arunee Wongkaew ◽  
Koki Asayama ◽  
Taisuke Kitaiwa ◽  
Shin-Ichi Nakamura ◽  
Katsuhiro Kojima ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Long Distance ◽  
Long Distance Transport ◽  
Protein Functions ◽  
Distance Transport

The Fine Structure of Phloem Cells

1985 ◽  
Vol 43 ◽  
pp. 632-635
Author(s):  
James Cronshaw
Keyword(s):  
Structural Studies ◽  
High Concentration ◽  
Long Distance ◽  
Phloem Tissue ◽  
Sieve Elements ◽  
Long Distance Transport ◽  
P Protein ◽  
Companion Cells ◽  
Electron Microscopical ◽  
Distance Transport

Long distance transport in plants takes place in phloem tissue which has characteristic cells, the sieve elements. At maturity these cells have sieve areas in their end walls with specialized perforations. They are associated with companion cells, parenchyma cells, and in some species, with transfer cells. The protoplast of the functioning sieve element contains a high concentration of sugar, and consequently a high hydrostatic pressure, which makes it extremely difficult to fix mature sieve elements for electron microscopical observation without the formation of surge artifacts. Despite many structural studies which have attempted to prevent surge artifacts, several features of mature sieve elements, such as the distribution of P-protein and the nature of the contents of the sieve area pores, remain controversial.

scholarly journals Comparative Analysis of Long-Distance Transportation with the Example of Sea and Rail Transport

Energies ◽  
2021 ◽  
Vol 14 (6) ◽  
pp. 1689
Author(s):  
Tomasz Neumann
Keyword(s):  
Eastern China ◽  
Time Cost ◽  
Topsis Method ◽  
Long Distance ◽  
Long Distance Transport ◽  
The Subject ◽  
Transport Analysis ◽  
Multi Criteria Analysis ◽  
The Relationship ◽  
Distance Transport

The subject of the article is a comparative long-distance transport analysis based on the relationship between central and eastern China and Poland. It provides an overview of issues related to long-haul China–Poland. The technique for order of preference by similarity to ideal solution (TOPSIS) method was proposed in the multi-criteria analysis. This method was briefly discussed, and its choice was justified. Then, the criteria adopted in the analysis were presented, i.e., time, cost, maximum number of containers, and ecology index. Multi-criteria analysis was carried out for three cases: the transport of one loading unit, 82 loading units, and 200 loading units. The geopolitical and operational situation on the transport route for the analyzed modes of transport was discussed.

scholarly journals Selecting sorting centres to avoid long distance transport of weaned beef calves

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
T. Morel-Journel ◽  
E. Vergu ◽  
J.-B. Mercier ◽  
N. Bareille ◽  
P. Ezanno
Keyword(s):  
Negative Impact ◽  
Historical Record ◽  
Management Tool ◽  
Long Distance ◽  
Beef Calves ◽  
Long Distance Transport ◽  
And Performance ◽  
Distance Transport ◽  
French Beef ◽  
General Concern

AbstractThe transport of weaned calves from cow–calf producers to fatteners is a general concern for the young bull industry due to its documented negative impact on the welfare, health and performance of the animals. These transfers are often managed by intermediaries who transport weaned calves to sorting centres, where they are grouped into batches before being sent to fattening units. In this study, we present an algorithm to limiting these transfer distances by appropriately selecting the sorting centre through which they must go. We tested the effectiveness of this algorithm on historical data from a French beef producer organization managing 136,892 transfers using 13 sorting centres. The results show a decrease in the transfer distances compared to the historical record, especially for the calves travelling over long distances (− 76 km, i.e. 18% on average for the 33% longest transfers). Moreover, the distribution of calves between the sorting centres proposed by the algorithm reveals differences in their efficiency in minimizing transfer distances. In addition to its usefulness as a management tool for the daily transport of cattle, this algorithm provides prospects for improving the management of the sorting centres themselves.

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