scholarly journals Spatiotemporal control of miR398 biogenesis via chromatin remodeling and kinase signaling ensures proper ovule development

2021 ◽  
Author(s):  
Hanyang Cai ◽  
Liping Liu ◽  
Man Zhang ◽  
Mengnan Chai ◽  
Youmei Huang ◽  
...  
Keyword(s):  
Chromatin Remodeling ◽  
Female Gametophyte ◽  
Early Stage ◽  
Mirna Biogenesis ◽  
Ovule Development ◽  
Kinase Signaling ◽  
Control Female ◽  
Microrna Gene ◽  
Receptor Kinase Signaling ◽  
Spatiotemporal Control

Abstract The coordinated development of sporophytic and gametophytic tissues is essential for proper ovule patterning and fertility. However, the mechanisms regulating their integrated development remain poorly understood. Here, we report that the Swi2/Snf2-Related1 (SWR1) chromatin remodeling complex acts with the ERECTA receptor kinase signaling pathway to control female gametophyte and integument growth in Arabidopsis thaliana by inhibiting transcription of the microRNA gene MIR398c in early-stage megagametogenesis. Moreover, pri-miR398c is transcribed in the female gametophyte but is then translocated to and processed in the ovule sporophytic tissues. Together, SWR1 and ERECTA also activate ARGONAUTE10 (AGO10) expression in the chalaza; AGO10 sequesters miR398, thereby ensuring the expression of three AGAMOUS-LIKE (AGL) genes (AGL51, AGL52, and AGL78) in the female gametophyte. In the context of sexual organ morphogenesis, these findings suggest that the spatiotemporal control of miRNA biogenesis, resulting from coordination between chromatin remodeling and cell signaling, is essential for proper ovule development in Arabidopsis.

scholarly journals The mac1 Gene: Controlling the Commitment to the Meiotic Pathway in Maize

Genetics ◽  
1996 ◽  
Vol 142 (3) ◽  
pp. 1009-1020 ◽  
Author(s):  
William F Sheridan ◽  
Nadezhda A Avalkina ◽  
Ivan I Shamrov ◽  
Tatyana B Batyea ◽  
Inna N Golubovskaya
Keyword(s):  
Female Gametophyte ◽  
Embryo Sac ◽  
Flowering Plants ◽  
Normal Female ◽  
Ovule Development ◽  
Partial Sterility ◽  
Embryo Sac Formation ◽  
Female Gametophyte Development ◽  
Normal Meiosis ◽  
Pleiotropic Mutation

Abstract The switch from the vegetative to the reproductive pathway of development in flowering plants requires the commitment of the subepidermal cells of the ovules and anthers to enter the meiotic pathway. These cells, the hypodermal cells, either directly or indirectly form the archesporial cells that, in turn, differentiate into the megasporocytes and microsporocytes. We have isolated a recessive pleiotropic mutation that we have termed multiple archesporial cells1 (macl) and located it to the short arm of chromosome 10. Its cytological phenotype suggests that this locus plays an important role in the switch of the hypodermal cells from the vegetative to the meiotic (sporogenous) pathway in maize ovules. During normal ovule development in maize, only a single hypodermal cell develops into an archesporial cell and this differentiates into the single megasporocyte. In macl mutant ovules several hypodermal cells develop into archesporial cells, and the resulting megasporocytes undergo a normal meiosis. More than one megaspore survives in the tetrad and more than one embryo sac is formed in each ovule. Ears on mutant plants show partial sterility resulting from abnormalities in megaspore differentiation and embryo sac formation. The sporophytic expression of this gene is therefore also important for normal female gametophyte development.

scholarly journals Myogenic MicroRNA Expression Requires ATP-Dependent Chromatin Remodeling Enzyme Function

2010 ◽  
Vol 30 (13) ◽  
pp. 3176-3186 ◽  
Author(s):  
Chandrashekara Mallappa ◽  
Brian T. Nasipak ◽  
Letitiah Etheridge ◽  
Elliot J. Androphy ◽  
Stephen N. Jones ◽  
...  
Keyword(s):  
Chromatin Remodeling ◽  
Ex Vivo ◽  
Chromatin Accessibility ◽  
Microrna Expression ◽  
Atpase Subunit ◽  
Chromatin Remodelers ◽  
Actin Organization ◽  
Microrna Gene ◽  
Tissue Culture Model ◽  
Microrna Processing

ABSTRACT Knockdown of the Brg1 ATPase subunit of SWI/SNF chromatin remodeling enzymes in developing zebrafish caused stunted tail formation and altered sarcomeric actin organization, which phenocopies the loss of the microRNA processing enzyme Dicer, or the knockdown of myogenic microRNAs. Furthermore, myogenic microRNA expression and differentiation was blocked in Brg1 conditional myoblasts differentiated ex vivo. The binding of Brg1 upstream of myogenic microRNA sequences correlated with MyoD binding and accessible chromatin structure in satellite cells and myofibers, and it was required for chromatin accessibility and microRNA expression in a tissue culture model for myogenesis. The results implicate ATP-dependent chromatin remodelers in myogenic microRNA gene regulation.

scholarly journals Emerging mechanisms to fine-tune receptor kinase signaling specificity

2020 ◽  
Vol 57 ◽  
pp. 41-51 ◽  
Author(s):  
Sergio Galindo-Trigo ◽  
Patrick Blümke ◽  
Rüdiger Simon ◽  
Melinka A Butenko
Keyword(s):  
Receptor Kinase ◽  
Kinase Signaling ◽  
Signaling Specificity ◽  
Receptor Kinase Signaling ◽  
Fine Tune

scholarly journals Organization of the Receptor-Kinase Signaling Array That RegulatesEscherichia coliChemotaxis

2002 ◽  
Vol 277 (39) ◽  
pp. 36748-36754 ◽  
Author(s):  
Mikhail N. Levit ◽  
Thorsten W. Grebe ◽  
Jeffry B. Stock
Keyword(s):  
Receptor Kinase ◽  
Kinase Signaling ◽  
Receptor Kinase Signaling

Receptor Kinase Signaling Pathways in Plant-Microbe Interactions

2012 ◽  
Vol 50 (1) ◽  
pp. 451-473 ◽  
Author(s):  
Meritxell Antolín-Llovera ◽  
Martina K. Ried ◽  
Andreas Binder ◽  
Martin Parniske
Keyword(s):  
Signaling Pathways ◽  
Receptor Kinase ◽  
Kinase Signaling ◽  
Microbe Interactions ◽  
Receptor Kinase Signaling

scholarly journals RNA-seq Analysis Reveals Gene Expression Profiling of Female Fertile and Sterile Ovules of Pinus Tabulaeformis Carr. during Free Nuclear Mitosis of the Female Gametophyte

2018 ◽  
Vol 19 (8) ◽  
pp. 2246 ◽  
Author(s):  
Yang Yao ◽  
Rui Han ◽  
Zaixin Gong ◽  
Caixia Zheng ◽  
Yuanyuan Zhao
Keyword(s):  
Gene Expression ◽  
Female Gametophyte ◽  
Expression Profiles ◽  
Signal Transduction Pathway ◽  
Gene Expression Profiles ◽  
Pinus Tabulaeformis ◽  
Cdna Libraries ◽  
Pcr Analysis ◽  
Transcriptional Networks ◽  
Ovule Development

The development of the female gametophyte (FG) is one of the key processes of life cycle alteration between the haploid gametophyte and the diploid sporophytes in plants and it is required for successful seed development after fertilization. It is well demonstrated that free nuclear mitosis (FNM) of FG is crucial for the development of the ovule. However, studies of the molecular mechanism of ovule and FG development focused mainly on angiosperms, such as Arabidopsis thaliana and further investigation of gymnosperms remains to be completed. Here, Illumina sequencing of six transcriptomic libraries obtained from developing and abortive ovules at different stages during free nuclear mitosis of magagametophyte (FNMM) was used to acquire transcriptome data and gene expression profiles of Pinus tabulaeformis. Six cDNA libraries generated a total of 71.0 million high-quality clean reads that aligned with 63,449 unigenes and the comparison between developing and abortive ovules identified 7174 differentially expressed genes (DEGs). From the functional annotation results, DEGs involved in the cell cycle and phytohormone regulation were highlighted to reveal their biological importance in ovule development. Furthermore, validation of DEGs from the phytohormone signal transduction pathway was performed using quantitative real-time PCR analysis, revealing the dynamics of transcriptional networks and potential key components in the regulation of FG development in P. tabulaeformis were identified. These findings provide new insights into the regulatory mechanisms of ovule development in woody gymnosperms.

Sign in / Sign up

Export Citation Format

Share Document