scholarly journals Chickpea WRKY70 Regulates the Expression of a Homeodomain-Leucine Zipper (HD-Zip) I Transcription Factor CaHDZ12, which Confers Abiotic Stress Tolerance in Transgenic Tobacco and Chickpea

2017 ◽  
Vol 58 (11) ◽  
pp. 1934-1952 ◽  
Author(s):  
Senjuti Sen ◽  
Joydeep Chakraborty ◽  
Prithwi Ghosh ◽  
Debabrata Basu ◽  
Sampa Das
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Leucine Zipper ◽  
Abiotic Stress Tolerance

scholarly journals TheSsDREBTranscription Factor from the Succulent HalophyteSuaeda salsaEnhances Abiotic Stress Tolerance in Transgenic Tobacco

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Xu Zhang ◽  
Xiaoxue Liu ◽  
Lei Wu ◽  
Guihong Yu ◽  
Xiue Wang ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Drought Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Abiotic Stress Tolerance ◽  
Soluble Sugars ◽  
Sequence Alignments ◽  
Multiple Sequence ◽  
Dreb Transcription Factor

Dehydration-responsive element-binding (DREB) transcription factor (TF) plays a key role for abiotic stress tolerance in plants. In this study, a novel cDNA encoding DREB transcription factor, designatedSsDREB, was isolated from succulent halophyteSuaeda salsa. This protein was classified in the A-6 group of DREB subfamily based on multiple sequence alignments and phylogenetic characterization. Yeast one-hybrid assays showed that SsDREB protein specifically binds to the DRE sequence and could activate the expression of reporter genes in yeast, suggesting that the SsDREB protein was a CBF/DREB transcription factor. Real-time RT-PCR showed thatSsDREBwas significantly induced under salinity and drought stress. Overexpression ofSsDREBcDNA in transgenic tobacco plants exhibited an improved salt and drought stress tolerance in comparison to the nontransformed controls. The transgenic plants revealed better growth, higher chlorophyll content, and net photosynthesis rate, as well as higher level of proline and soluble sugars. The semiquantitative PCR of transgenics showed higher expression of stress-responsive genes. These data suggest that theSsDREBtranscription factor is involved in the regulation of salt stress tolerance in tobacco by the activation of different downstream gene expression.

Ectopic expression of a fruit phytoene synthase from Citrus paradisi Macf. promotes abiotic stress tolerance in transgenic tobacco

2012 ◽  
Vol 39 (12) ◽  
pp. 10201-10209 ◽  
Author(s):  
Luciana C. Cidade ◽  
Tahise M. de Oliveira ◽  
Amanda F. S. Mendes ◽  
Amanda F. Macedo ◽  
Eny I. S. Floh ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Transgenic Tobacco ◽  
Abiotic Stress Tolerance ◽  
Ectopic Expression ◽  
Phytoene Synthase ◽  
Citrus Paradisi

Heterologous Overexpression of ZmHDZIV13 Enhanced Drought and Salt Tolerance in Arabidopsis and Tobacco

2021 ◽  
Author(s):  
Fang Wang ◽  
Peng Fang ◽  
Huiping Yan ◽  
Xiangzhuo Ji ◽  
Yunling Peng
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Salt Tolerance ◽  
Leucine Zipper ◽  
Abiotic Stress Tolerance ◽  
Heterologous Overexpression ◽  
Malondialdehyde Content ◽  
Drought And Salt Tolerance ◽  
Relative Water ◽  
Dependent Signaling Pathway

Abstract The homeodomain leucine zipper (HD-Zip) IV transcription factor is indispensable in the response of plants to abiotic stress. Systematic studies have been carried out in Arabidopsis, rice and other species from which a series of stress resistance-related genes have been isolated. However, the function of the HD-Zip-IV protein in maize is not clear. In this study, we cloned the HD-Zip-IV gene ZmHDZIV13 and identified its function in the stress response. Our phylogenetic analysis showed that ZmHDZIV13 and AtHDG11 had high homology and might have similar functions. The heterologous overexpression of ZmHDZIV13 in Arabidopsis resulted in sensitivity to abscisic acid (ABA), salt tolerance during germination and drought tolerance in seedlings. Under drought stress, the transgenic Arabidopsis showed stronger drought resistance than the wild-type showed (control). The malondialdehyde content of ZmHDZIV13 transgenic plants was lower than that of the control, and the relative water content and proline content were significantly higher than those of the control. After the drought was relieved, the expression of P5CS1, RD22, RD29B, RD29A, NCED3 and ERD1 were upregulated in transgenic Arabidopsis. Also, modified tobacco plants (35S::ZmHDZIV13) exhibited proper stomatal changes in response to drought conditions. These results show that ZmHDZIV13, as a stress-responsive transcription factor, plays a role in the positive regulation of abiotic stress tolerance and can regulate an ABA-dependent signaling pathway to regulate drought response in plants.

Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis

2015 ◽  
Vol 35 (3) ◽  
pp. 655-666 ◽  
Author(s):  
Yangyang Yuan ◽  
Linchuan Fang ◽  
Sospeter Karanja Karungo ◽  
Langlang Zhang ◽  
Yingying Gao ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Stress Tolerance ◽  
Abiotic Stress Tolerance ◽  
Vitis Amurensis ◽  
Gras Transcription Factor
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