scholarly journals Enhanced Ascorbate Regeneration Via Dehydroascorbate Reductase Confers Tolerance to Photo-Oxidative Stress inChlamydomonas reinhardtii

2016 ◽  
Vol 57 (10) ◽  
pp. 2104-2121 ◽  
Author(s):  
Shu-Tseng Lin ◽  
Chih-Wen Chiou ◽  
Yen-Lin Chu ◽  
Yu Hsiao ◽  
Yu-Fei Tseng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dehydroascorbate Reductase ◽  
Ascorbate Regeneration

Effect of peroxynitrite on glutaredoxin

2001 ◽  
Vol 20 (7) ◽  
pp. 373-376 ◽  
Author(s):  
G Aykaç-Toker ◽  
S Bulgurcuogélu ◽  
N Koçak-Toker
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Ascorbic Acid ◽  
Rat Liver ◽  
Dehydroascorbate Reductase ◽  
Active Elements ◽  
Thiol Homeostasis ◽  
Important Enzyme

Glutaredoxin is an important enzyme in thiol homeostasis. As a thioltransferase, it reduces oxidized thiols. It also has dehydroascorbate reductase (DHAR) activity to reduce dehydroascorbate (DHA) to ascorbic acid. Peroxynitrite (ONOO-) is one of the most active elements of oxidative stress that can be formed wherever nitric oxide and superoxide are produced simultaneously. ONOO- is known to react with free thiols easily. To observe the effect of ONOO- on glutaredoxin, rat liver cytosolic fractions were incubated with 0–250 MONOO -. Thioltransferase activity was found to be decreased as ONOO- concentration increased. The inhibition was not reversible with dithiothreitol (DTT). In cytosol besides glutaredoxin, another enzyme with DHAR activity is also present. In our study, the cytosolic DHAR activity which consisted both enzymes, was also inhibited by ONOO-, but DTT was able to return the activity almost completely.

scholarly journals Dehydroascorbate and dehydroascorbate reductase are phantom indicators of oxidative stress in plants

FEBS Letters ◽  
1997 ◽  
Vol 414 (3) ◽  
pp. 567-570 ◽  
Author(s):  
Susanne Morell ◽  
Hartmut Follmann ◽  
Mario De Tullio ◽  
Ingo Häberlein
Keyword(s):  
Oxidative Stress ◽  
Dehydroascorbate Reductase

scholarly journals Over-expression of dehydroascorbate reductase enhances oxidative stress tolerance in tobacco

2017 ◽  
Vol 25 ◽  
pp. 1-8 ◽  
Author(s):  
Lingmo Chang ◽  
Huan Sun ◽  
Hua Yang ◽  
Xuehua Wang ◽  
Zhizhe Su ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Tolerance ◽  
Dehydroascorbate Reductase ◽  
Oxidative Stress Tolerance ◽  
Over Expression

Insights into ascorbate regeneration in plants: investigating the redox and structural properties of dehydroascorbate reductases from Populus trichocarpa

2016 ◽  
Vol 473 (6) ◽  
pp. 717-731 ◽  
Author(s):  
Pierre-Alexandre Lallement ◽  
Thomas Roret ◽  
Pascale Tsan ◽  
José M. Gualberto ◽  
Jean-Michel Girardet ◽  
...  
Keyword(s):  
Kinetic Analysis ◽  
Structural Properties ◽  
Populus Trichocarpa ◽  
Nmr Structure ◽  
Dehydroascorbate Reductase ◽  
Cysteine Residues ◽  
Redox States ◽  
Mediated Oxidation ◽  
Ascorbate Regeneration ◽  
Dehydroascorbate Reduction

The resolution of a NMR structure of a poplar dehydroascorbate reductase (DHAR) coupled to kinetic analysis and GSSG-mediated oxidation treatments revealed that DHARs are highly specialized for dehydroascorbate reduction and adopt different redox states depending on the number of cysteine residues.

Lycopene alleviates sulfamethoxazole-induced hepatotoxicity in grass carp (Ctenopharyngodon idellus) via suppression of oxidative stress, inflammation and apoptosis

2020 ◽  
Vol 11 (10) ◽  
pp. 8547-8559
Author(s):  
Hongjing Zhao ◽  
Yu Wang ◽  
Mengyao Mu ◽  
Menghao Guo ◽  
Hongxian Yu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Secondary Metabolites ◽  
Human Health ◽  
Grass Carp ◽  
Aquatic Environment ◽  
Ctenopharyngodon Idellus

Antibiotics are used worldwide to treat diseases in humans and other animals; most of them and their secondary metabolites are discharged into the aquatic environment, posing a serious threat to human health.

Copper-dependent ATP7B up-regulation drives the resistance of TMEM16A-overexpressing head-and-neck cancer models to platinum toxicity

2019 ◽  
Vol 476 (24) ◽  
pp. 3705-3719 ◽  
Author(s):  
Avani Vyas ◽  
Umamaheswar Duvvuri ◽  
Kirill Kiselyov
Keyword(s):  
Oxidative Stress ◽  
Head And Neck ◽  
Transcriptional Activation ◽  
Platinum Resistance ◽  
Mrna Levels ◽  
Strong Positive Correlation ◽  
Platinum Compounds ◽  
Copper Chelation ◽  
Novel Approach ◽  
Cancer Models

Platinum-containing drugs such as cisplatin and carboplatin are routinely used for the treatment of many solid tumors including squamous cell carcinoma of the head and neck (SCCHN). However, SCCHN resistance to platinum compounds is well documented. The resistance to platinum has been linked to the activity of divalent transporter ATP7B, which pumps platinum from the cytoplasm into lysosomes, decreasing its concentration in the cytoplasm. Several cancer models show increased expression of ATP7B; however, the reason for such an increase is not known. Here we show a strong positive correlation between mRNA levels of TMEM16A and ATP7B in human SCCHN tumors. TMEM16A overexpression and depletion in SCCHN cell lines caused parallel changes in the ATP7B mRNA levels. The ATP7B increase in TMEM16A-overexpressing cells was reversed by suppression of NADPH oxidase 2 (NOX2), by the antioxidant N-Acetyl-Cysteine (NAC) and by copper chelation using cuprizone and bathocuproine sulphonate (BCS). Pretreatment with either chelator significantly increased cisplatin's sensitivity, particularly in the context of TMEM16A overexpression. We propose that increased oxidative stress in TMEM16A-overexpressing cells liberates the chelated copper in the cytoplasm, leading to the transcriptional activation of ATP7B expression. This, in turn, decreases the efficacy of platinum compounds by promoting their vesicular sequestration. We think that such a new explanation of the mechanism of SCCHN tumors’ platinum resistance identifies novel approach to treating these tumors.

Clinical aspects of reactive oxygen and nitrogen species

2004 ◽  
Vol 71 ◽  
pp. 121-133 ◽  
Author(s):  
Ascan Warnholtz ◽  
Maria Wendt ◽  
Michael August ◽  
Thomas Münzel
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Risk Factor ◽  
Endothelial Dysfunction ◽  
Vascular Tissue ◽  
Rapid Development ◽  
Reactive Oxygen ◽  
Clinical Aspects ◽  
No Production ◽  
Oxygen Species

Endothelial dysfunction in the setting of cardiovascular risk factors, such as hypercholesterolaemia, hypertension, diabetes mellitus and chronic smoking, as well as in the setting of heart failure, has been shown to be at least partly dependent on the production of reactive oxygen species in endothelial and/or smooth muscle cells and the adventitia, and the subsequent decrease in vascular bioavailability of NO. Superoxide-producing enzymes involved in increased oxidative stress within vascular tissue include NAD(P)H-oxidase, xanthine oxidase and endothelial nitric oxide synthase in an uncoupled state. Recent studies indicate that endothelial dysfunction of peripheral and coronary resistance and conductance vessels represents a strong and independent risk factor for future cardiovascular events. Ways to reduce endothelial dysfunction include risk-factor modification and treatment with substances that have been shown to reduce oxidative stress and, simultaneously, to stimulate endothelial NO production, such as inhibitors of angiotensin-converting enzyme or the statins. In contrast, in conditions where increased production of reactive oxygen species, such as superoxide, in vascular tissue is established, treatment with NO, e.g. via administration of nitroglycerin, results in a rapid development of endothelial dysfunction, which may worsen the prognosis in patients with established coronary artery disease.

Pancreatic stones shockwave lithotripsy induces oxidative stress. A study of lipoperoxidation products in pure pancreatic juice

2001 ◽  
Vol 120 (5) ◽  
pp. A217-A217
Author(s):  
C SPADA ◽  
S SANTINI ◽  
F FOSCHIA ◽  
M PANDOLFI ◽  
V PERRI ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pancreatic Juice ◽  
Shockwave Lithotripsy ◽  
Pure Pancreatic Juice ◽  
Pancreatic Stones
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