scholarly journals Differential Contribution of Endoplasmic Reticulum and Chloroplast ω-3 Fatty Acid Desaturase Genes to the Linolenic Acid Content of Olive (Olea europaea) Fruit

2015 ◽  
Vol 57 (1) ◽  
pp. 138-151 ◽  
Author(s):  
M. Luisa Hernández ◽  
M. Dolores Sicardo ◽  
José M. Martínez-Rivas
Keyword(s):  
Endoplasmic Reticulum ◽  
Fatty Acid ◽  
Linolenic Acid ◽  
Acid Content ◽  
Olea Europaea ◽  
Fatty Acid Desaturase ◽  
Linolenic Acid Content ◽  
Differential Contribution ◽  
Olea Europaéa

New layers in understanding and predicting α-linolenic acid content in plants using amino acid characteristics of omega-3 fatty acid desaturase

2014 ◽  
Vol 54 ◽  
pp. 14-23 ◽  
Author(s):  
Zahra Zinati ◽  
Fatemeh Zamansani ◽  
Amir Hossein KayvanJoo ◽  
Mahdi Ebrahimi ◽  
Mansour Ebrahimi ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Linolenic Acid ◽  
Acid Content ◽  
Fatty Acid Desaturase ◽  
Linolenic Acid Content ◽  
Omega 3 ◽  
Omega 3 Fatty Acid

Omega-3 fatty acid desaturase (FAD3, FAD7, FAD8) gene expression and linolenic acid content in cowpea leaves submitted to drought and after rehydration

2009 ◽  
Vol 65 (2-3) ◽  
pp. 162-169 ◽  
Author(s):  
Maria-Lucia Torres-Franklin ◽  
Anne Repellin ◽  
Van-Biet Huynh ◽  
Agnès d’Arcy-Lameta ◽  
Yasmine Zuily-Fodil ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acid ◽  
Linolenic Acid ◽  
Acid Content ◽  
Fatty Acid Desaturase ◽  
Linolenic Acid Content ◽  
Omega 3 ◽  
Omega 3 Fatty Acid

Effect of transplanting date on the fatty acid composition, oil content and yield of evening primrose (Oenothera biennis L.) seed

1994 ◽  
Vol 74 (1) ◽  
pp. 129-131 ◽  
Author(s):  
Robert C. Roy ◽  
Peter H. White ◽  
Alex F. More ◽  
John G. Hendel ◽  
Robert Pocs ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Key Words ◽  
Linolenic Acid ◽  
Acid Content ◽  
Oil Content ◽  
Linolenic Acid Content ◽  
Oenothera Biennis ◽  
Evening Primrose ◽  
One Year

The influence of transplanting time on the growth of evening primrose (Oenothera biennis L.) was investigated for 3 yr. Delaying transplanting reduced the yield of seed in two of these years. The amount of oil in the seed was not influenced by time of transplanting, and only in one year did the γ-linolenic acid content of the oil decrease with delays in transplanting. Key words: Evening primrose, transplanting date, oil content, γ-linolenic acid

scholarly journals Heterologous Production of Dihomo-γ-Linolenic Acid in Saccharomyces cerevisiae

2007 ◽  
Vol 73 (21) ◽  
pp. 6965-6971 ◽  
Author(s):  
Hisashi Yazawa ◽  
Hitoshi Iwahashi ◽  
Yasushi Kamisaka ◽  
Kazuyoshi Kimura ◽  
Tsunehiro Aki ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Linolenic Acid ◽  
Acid Content ◽  
Kluyveromyces Lactis ◽  
Fatty Acid Content ◽  
Fatty Acid Desaturase ◽  
Dry Weight ◽  
Medium Composition

ABSTRACT To make dihomo-γ-linolenic acid (DGLA) (20:3n-6) in Saccharomyces cerevisiae, we introduced Kluyveromyces lactis Δ12 fatty acid desaturase, rat Δ6 fatty acid desaturase, and rat elongase genes. Because Fad2p is able to convert the endogenous oleic acid to linoleic acid, this allowed DGLA biosynthesis without the need to supply exogenous fatty acids on the media. Medium composition, cultivation temperature, and incubation time were examined to improve the yield of DGLA. Fatty acid content was increased by changing the medium from a standard synthetic dropout medium to a nitrogen-limited minimal medium (NSD). Production of DGLA was higher in the cells grown at 15�C than in those grown at 20�C, and no DGLA production was observed in the cells grown at 30�C. In NSD at 15�C, fatty acid content increased up until day 7 and decreased after day 10. When the cells were grown in NSD for 7 days at 15�C, the yield of DGLA reached 2.19 μg/mg of cells (dry weight) and the composition of DGLA to total fatty acids was 2.74%. To our knowledge, this is the first report describing the production of polyunsaturated fatty acids in S. cerevisiae without supplying the exogenous fatty acids.

scholarly journals Training status diverges muscle diacylglycerol accumulation during free fatty acid elevation

2014 ◽  
Vol 307 (1) ◽  
pp. E124-E131 ◽  
Author(s):  
Lisa S. Chow ◽  
Douglas G. Mashek ◽  
Erin Austin ◽  
Lynn E. Eberly ◽  
Xuan-Mai Persson ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Insulin Sensitivity ◽  
Free Fatty Acid ◽  
Linolenic Acid ◽  
Acid Content ◽  
Training Status ◽  
Linolenic Acid Content ◽  
Lipid Infusion ◽  
Palmitoyl Carnitine ◽  
Liquid Chromatography Tandem Mass

How endurance training alters muscle lipid metabolism while preserving insulin sensitivity remains unclear. Because acute free fatty acid (FFA) elevation by lipid infusion reduces insulin sensitivity, we hypothesized that training status would alter accumulation of muscle triacylglycerol (TAG), diacylglycerol (DAG), ceramide, and acylcarnitine during acute FFA elevation. Trained ( n = 15) and sedentary ( n = 13) participants matched for age, sex, and BMI received either a 6-h infusion of lipid (20% Intralipid at 90 ml/h) or glycerol (2.25 g/100 ml at 90 ml/h) during a hyperinsulinemic euglycemic clamp. Muscle biopsies were taken at 0, 120, and 360 min after infusion initiation to measure intramyocellular concentrations of TAG, DAG, ceramides, and acylcarnitines by liquid chromatography-tandem mass spectrometry. Trained participants had a higher V̇o2 max and insulin sensitivity than sedentary participants. The lipid infusion produced a comparable elevation of FFA (594 ± 90 μmol/l in trained, 721 ± 30 μmol/l in sedentary, P = 0.4) and a decline in insulin sensitivity (−44.7% trained vs. −47.2% sedentary, P = 0.89). In both groups, lipid infusion increased the linoleic and linolenic acid content of TAG without changing total TAG. In the sedentary group, lipid infusion increased total, oleic, and linoleic acid and linolenic acid content of DAG. Regardless of training status, lipid infusion did not alter total ceramide, saturated ceramide, palmitoyl-carnitine, or oleoyl-carnitine. We conclude that during acute FFA elevation, trained adults have a similar decline in insulin sensitivity with less accumulation of muscle DAG than sedentary adults, suggesting that lipid-induced insulin resistance can occur without elevation of total muscle DAG.

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