scholarly journals Stress Tolerance Profiling of a Collection of Extant Salt-Tolerant Rice Varieties and Transgenic Plants Overexpressing Abiotic Stress Tolerance Genes

2015 ◽  
Vol 56 (10) ◽  
pp. 1867-1876 ◽  
Author(s):  
Ken-ichi Kurotani ◽  
Kazumasa Yamanaka ◽  
Yosuke Toda ◽  
Daisuke Ogawa ◽  
Maiko Tanaka ◽  
...  
2020 ◽  
pp. 139-173
Author(s):  
Chandra Obul Reddy Puli ◽  
Chandra Sekhar Akila ◽  
Varakumar Pandit ◽  
Sravani Konduru ◽  
Suresh Raju Kandi ◽  
...  

2011 ◽  
Vol 58 (7) ◽  
pp. 693-721 ◽  
Author(s):  
Syed Sarfraz Hussain ◽  
Hasnain Raza ◽  
Irfan Afzal ◽  
Mahmood Akhtar Kayani

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Supratim Basu ◽  
Aryadeep Roychoudhury

The present study considered transcriptional profiles and protein expression analyses from shoot and/or root tissues under three abiotic stress conditions, namely, salinity, dehydration, and cold, as well as following exogenous abscisic acid treatment, at different time points of stress exposure in threeindicarice varieties, IR-29 (salt sensitive), Pokkali, and Nonabokra (both salt tolerant). The candidate genes chosen for expression studies wereHKT-1, SOS-3, NHX-1, SAPK5, SAPK7, NAC-1, Rab16A, OSBZ8, DREBP2, CRT/DREBP, WRKY24,andWRKY71, along with the candidate proteins OSBZ8, SAMDC, and GST. Gene expression profile revealed considerable differences between the salt-sensitive and salt-tolerant rice varieties, as the expression in the latter was higher even at the constitutive level, whereas it was inducible only by corresponding stress signals in IR-29. Whether in roots or shoots, the transcriptional responses to different stressors peaked following 24 h of stress/ABA exposure, and the transcript levels enhanced gradually with the period of exposure. The generality of stress responses at the transcriptional level was therefore time dependent. Heat map data also showed differential transcript abundance in the three varieties, correlating the observation with transcript profiling.In silicoanalysis of the upstream regions of all the genes represented the existence of conserved sequence motifs in single or multiple copies that are indispensable to abiotic stress response. Overall, the transcriptome and proteome analysis undertaken in the present study indicated that genes/proteins conferring tolerance, belonging to different functional classes, were overrepresented, thus providing novel insight into the functional basis of multiple stress tolerance inindicarice varieties. The present work will pave the way in future to select gene(s) for overexpression, so as to generate broad spectrum resistance to multiple stresses simultaneously.


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