scholarly journals Methyl Jasmonate Affects Morphology, Number and Activity of Endoplasmic Reticulum Bodies in Raphanus sativus Root Cells

2014 ◽  
Vol 56 (1) ◽  
pp. 61-72 ◽  
Author(s):  
Maxime Gotté ◽  
Rajgourab Ghosh ◽  
Sophie Bernard ◽  
Eric Nguema-Ona ◽  
Maïté Vicré-Gibouin ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Methyl Jasmonate ◽  
Raphanus Sativus ◽  
Root Cells

scholarly journals POLYRIBOSOMES AND CISTERNAL ACCUMULATIONS IN ROOT CELLS OF RADISH

1965 ◽  
Vol 27 (2) ◽  
pp. 423-432 ◽  
Author(s):  
Howard T. Bonnett ◽  
Eldon H. Newcomb
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Raphanus Sativus ◽  
Phase Contrast ◽  
Animal Cells ◽  
Root Cells ◽  
Common Component ◽  
Raphanus Sativus L ◽  
Root Hair Formation ◽  
Hair Formation

The zone of root hair formation of seedling radish roots, Raphanus sativus L., was studied by phase-contrast and electron microscopy. Localized dilations of the endoplasmic reticulum, which contained a moderately dense proteinaceous material, were found to be a common component of the cytoplasm in cells of the epidermis and cortex. The surfaces of these dilations were covered with polyribosomes in discrete coils commonly composed of 15 to 17 ribosomes. The function of these structures and the fate of the material accumulated in them are unknown. Their similarity to structures described in some types of animal cells is discussed.

Methyl Jasmonate Inhibits Postharvest Sprouting of Radishes

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 458b-458
Author(s):  
Chien Y. Wang
Keyword(s):  
Weight Loss ◽  
Root Growth ◽  
Methyl Jasmonate ◽  
Raphanus Sativus ◽  
Secondary Effect

Treatment of topped radishes (Raphanus sativus L., cv. Cherry Belle) with methyl jasmonate was effective in inhibiting postharvest sprouting of new leaves and the growth of roots. Radishes were trimmed to 10-mm tops and dipped in various methyl jasmonate suspensions for 3 min. After storage at 15 °C for 7 days, the growths of new leaves were 26, 22, 7, 3, and 1 mm in 0, 10–5, 10–4, 10–3, or 2 × 10–3 M methyl jasmonate-treated radishes, respectively. The lengths of root growth were also reduced by methyl jasmonate particularly at higher concentrations. These treatments also substantially reduced weight loss possibly as a secondary effect. Fumigation with methyl jasmonate vapor in enclosed containers was also effective in inhibiting the sprouting of leaves and root growth, but to a lesser extent than dipping treatments. Radishes stored at 0 °C did not show any new growth of leaves or roots, and therefore were not affected by the methyl jasmonate treatments.

scholarly journals The Endoplasmic Reticulum and the Golgi Structures in Maize Root Cells

1959 ◽  
Vol 5 (3) ◽  
pp. 501-506 ◽  
Author(s):  
W. Gordon Whaley ◽  
Hilton H. Mollenhauer ◽  
Joyce E. Kephart
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Wall ◽  
Electron Microscope ◽  
Nuclear Envelope ◽  
Epoxy Resin ◽  
Maize Root ◽  
Root Tips ◽  
Animal Cells ◽  
Root Cells ◽  
Vesicular Structure

Maize root tips were fixed in potassium permanganate, embedded in epoxy resin, sectioned to show silver interference color, and studied with the electron microscope. All the cells were seen to contain an endoplasmic reticulum and apparently independent Golgi structures. The endoplasmic reticulum is demonstrated as a membrane-bounded, vesicular structure comparable in many aspects to that of several types of animal cells. With the treatment used here the membranes appear smooth surfaced. The endoplasmic reticulum is continuous with the nuclear envelope and, by contact at least, with structures passing through the cell wall. The nuclear envelope is characterized by discontinuities, as previously reported for animal cells. The reticula of adjacent cells seem to be in contact at or through the plasmodesmata. Because of these contacts the endoplasmic reticulum of a given cell appears to be part of an intercellular system. The Golgi structures appear as stacks of platelet-vesicles which apparently may, under certain conditions, produce small vesicles around their edges. Their form changes markedly with development of the cell.

scholarly journals THE STRUCTURE OF SOME CYTOPLASMIC COMPONENTS OF PLANT CELLS IN RELATION TO THE BIOCHEMICAL PROPERTIES OF ISOLATED PARTICLES

1957 ◽  
Vol 3 (1) ◽  
pp. 61-70 ◽  
Author(s):  
A. J. Hodge ◽  
E. M. Martin ◽  
R. K. Morton
Keyword(s):  
Endoplasmic Reticulum ◽  
Wheat Root ◽  
Dense Material ◽  
Animal Cells ◽  
Wheat Roots ◽  
Root Cells ◽  
Intact Cells ◽  
Thin Sections ◽  
Isolated Mitochondria ◽  
Silver Beet

1. Electron micrographs of thin sections of material fixed with buffered osmium tetroxide have been used for comparison of the fine structure of isolated cytoplasmic particles from silver beet petioles and roots of germinating wheat with that of the cytoplasm of the intact cells. 2. Mitochondria of wheat roots have an external double membrane and poorly oriented internal double membranes. As compared with the structures seen in situ, the isolated mitochondria showed evidence of some disorganisation of the fine internal structure, probably due to osmotic effects. The possible influence of such changes on the enzymic properties of the isolated mitochondria is discussed. 3. The isolated plant microsomes are mainly spherical vesicular structures consisting of (a) an outer membrane enclosing (b) either an homogeneous slightly dense material (wheat root microsomes) or some granular dense material (silver beet microsomes) and (c) small dense particles, mostly associated with the vesicle membranes. 4. The cytoplasm of the wheat root cells does not contain any structures similar to the isolated microsomes but has a very dense reticular network, consisting of membranes with associated small dense particles, here called the endoplasmic reticulum. The observations indicate that the isolated microsomes arise mainly by rupture and transformation of the membranes of this structure. The effects of such extensive changes in the lipoprotein membranes on the enzymic activities of the endoplasmic reticulum, as studied in isolated microsomes, is discussed. 5. Meristematic wheat root cells contain structures which consist of smooth membranes with associated vacuoles and are similar to the Golgi zones of animal cells. The membranes of these zones probably contribute to the microsomal fraction under the conditions of preparation used for the enzymic and chemical studies previously reported.

scholarly journals Analyses of Ca2+ Accumulation and Dynamics in the Endoplasmic Reticulum of Arabidopsis Root Cells Using a Genetically Encoded Cameleon Sensor

2013 ◽  
Vol 163 (3) ◽  
pp. 1230-1241 ◽  
Author(s):  
Maria Cristina Bonza ◽  
Giovanna Loro ◽  
Smrutisanjita Behera ◽  
Andrea Wong ◽  
Jörg Kudla ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Root Cells ◽  
Arabidopsis Root

scholarly journals The amounts and rates of export of polysaccharides found within the membrane system of maize root cells

1974 ◽  
Vol 142 (1) ◽  
pp. 139-144 ◽  
Author(s):  
Dianna J. Bowles ◽  
D. H. Northcote
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Wall ◽  
Golgi Apparatus ◽  
Cell Surface ◽  
Rough Endoplasmic Reticulum ◽  
Membrane System ◽  
Maize Root ◽  
Wall Material ◽  
Cell Wall Material ◽  
Root Cells

1. Maize seedling roots were incubated in vivo with d-[U-14C]glucose for 2, 5, 10, 15, 30 and 45min. The total incorporation of radioactivity into polysaccharide components in isolated fractions was investigated, and the pattern of incorporation into different polysaccharide components within the rough endoplasmic reticulum, Golgi apparatus and exported material was analysed. 2. The membrane compartments reached a saturation value of radioactivity in polysaccharide components by 30min incubation. Radioactivity in exported polysaccharide continued to increase after that time. The latter was formed and maintained by a steady-state turnover of polysaccharide synthesis and transport from the membrane system. 3. If the only access of the slime polysaccharide to the cell surface is via dictyosome-derived vesicles, the amount of slime components in the Golgi apparatus would have to be displaced every 0.3min in order to maintain the observed rates of increase in slime. This is in contrast with a displacement time of about 2.5min that is necessary for polysaccharide components in the Golgi apparatus to produce the observed increase in cell-wall material. The activity of the membrane system in the production of maize root slime is 8 times as great as that of the membrane system involved in cell-wall synthesis. 4. If the amount of polysaccharide material in the Golgi apparatus is maintained only by inflow of polymeric material from the rough endoplasmic reticulum the total amount of slime components in the rough endoplasmic reticulum would have to be displaced every 7min to maintain a constant amount in the Golgi apparatus. If the endoplasmic reticulum contributed directly to the cell surface in the synthesis of cell-wall material, displacement times necessary to maintain the observed rate of polymer production would be very slow.

scholarly journals High voltage electron microscopy of membrane interactions in wheat.

1980 ◽  
Vol 28 (10) ◽  
pp. 1129-1132 ◽  
Author(s):  
F Marty
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Membrane Flow ◽  
Root Cells ◽  
Selective Labeling ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron ◽  
Triticum Sativum ◽  
Zinc Iodide ◽  
Conventional Electron Microscopy

Structural interactions between intracellular membranes in root cells of Triticum sativum L. have been investigated with the 3 MeV electron microscope in Toulouse after selective labeling of the membranes with either zinc iodide-osmium tetroxide or phosphotungstate at low pH. Direct membrane continuities between endoplasmic reticulum, Golgi apparatus, and biogenetically derived compartments of the exoplasmic space are more extensive than commonly suggested from conventional electron microscopy. The results are discussed with reference to current concepts suggesting a membrane flow from the endoplasmic reticulum to the terminal components, plasmalemma, and vacuoles.

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