scholarly journals Tandem CCCH Zinc Finger Proteins in Plant Growth, Development and Stress Response

2014 ◽  
Vol 55 (8) ◽  
pp. 1367-1375 ◽  
Author(s):  
Srimathi P. Bogamuwa ◽  
Jyan-Chyun Jang
Keyword(s):  
Stress Response ◽  
Plant Growth ◽  
Zinc Finger ◽  
Zinc Finger Proteins ◽  
Ccch Zinc Finger ◽  
Growth Development ◽  
Tandem Ccch Zinc Finger

scholarly journals BcMF30a and BcMF30c, Two Novel Non-Tandem CCCH Zinc-Finger Proteins, Function in Pollen Development and Pollen Germination in Brassica campestris ssp. chinensis

2020 ◽  
Vol 21 (17) ◽  
pp. 6428
Author(s):  
Liai Xu ◽  
Xingpeng Xiong ◽  
Weimiao Liu ◽  
Tingting Liu ◽  
Youjian Yu ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Chinese Cabbage ◽  
Pollen Development ◽  
Male Fertility ◽  
Pollen Germination ◽  
Brassica Campestris ◽  
Reproductive Development ◽  
Zinc Finger Proteins ◽  
Ccch Zinc Finger ◽  
Tandem Ccch Zinc Finger

Chinese cabbage (Brassica campestris) is an economically important leaf vegetable crop worldwide. Mounting studies have shown that cysteine-cysteine-cysteine-histidine (CCCH) zinc-finger protein genes are involved in various plant growth and development processes. However, research on the involvement of these genes in male reproductive development is still in its infancy. Here, we identified 11 male fertility-related CCCH genes in Chinese cabbage. Among them, a pair of paralogs encoding novel non-tandem CCCH zinc-finger proteins, Brassica campestris Male Fertility 30a (BcMF30a) and BcMF30c, were further characterized. They were highly expressed in pollen during microgametogenesis and continued to express in germinated pollen. Further analyses demonstrated that both BcMF30a and BcMF30c may play a dual role as transcription factors and RNA-binding proteins in plant cells. Functional analysis showed that partial bcmf30a bcmf30c pollen grains were aborted due to the degradation of pollen inclusion at the microgametogenesis phase, and the germination rate of viable pollen was also greatly reduced, indicating that BcMF30a and BcMF30c are required for both pollen development and pollen germination. This research provided insights into the function of CCCH proteins in regulating male reproductive development and laid a theoretical basis for hybrid breeding of Chinese cabbage.

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