scholarly journals Identification of IAA Transport Inhibitors Including Compounds Affecting Cellular PIN Trafficking by Two Chemical Screening Approaches Using Maize Coleoptile Systems

2012 ◽  
Vol 53 (10) ◽  
pp. 1671-1682 ◽  
Author(s):  
Takeshi Nishimura ◽  
Naoyuki Matano ◽  
Taichi Morishima ◽  
Chieko Kakinuma ◽  
Ken-ichiro Hayashi ◽  
...  
Keyword(s):  
Chemical Screening ◽  
Maize Coleoptile ◽  
Transport Inhibitors ◽  
Screening Approaches

Discovery of New AKT1 Inhibitors by Combination of In silico Structure Based Virtual Screening Approaches and Biological Evaluations

2019 ◽  
Author(s):  
Filip Fratev ◽  
Denisse A. Gutierrez ◽  
Renato J. Aguilera ◽  
suman sirimulla
Keyword(s):  
Virtual Screening ◽  
Success Rate ◽  
Protein Interactions ◽  
In Silico ◽  
Biological Data ◽  
In Vitro Binding ◽  
Vitro Binding ◽  
Screening Protocol ◽  
Screening Approaches

AKT1 is emerging as a useful target for treating cancer. Herein, we discovered a new set of ligands that inhibit the AKT1, as shown by in vitro binding and cell line studies, using a newly designed virtual screening protocol that combines structure-based pharmacophore and docking screens. Taking together with the biological data, the combination of structure based pharamcophore and docking methods demonstrated reasonable success rate in identifying new inhibitors (60-70%) proving the success of aforementioned approach. A detail analysis of the ligand-protein interactions was performed explaining observed activities.<br>

UJI IMUNOSTIMULATOR DARI KOMBINASI EKSTRAK ETANOL HERBA BINARA (ARTEMISIA VULGARIS LINN) DAN EKSTRAK ETANOL DAUN PUCUK MERAH (SYZYGIUM OLEANA) DENGAN METODE HIPERSENSITIVITAS TIPE LAMBAT PADA TIKUS JANTAN

2019 ◽  
Vol 1 (2) ◽  
pp. 22-26
Author(s):  
Romauli Anna Teresia Marbun ◽  
Aminah Syarifuddin ◽  
Montysory Silalahi ◽  
Radika Bella Fista Ginting
Keyword(s):  
Antioxidant Activity ◽  
Immune System ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Positive Control ◽  
Artemisia Vulgaris ◽  
Chemical Screening ◽  
Chemical Content ◽  
High Antioxidant Activity ◽  
New Treatments

Diseases mediated by the immune system are difficult problems to treat such as human immunodeficiency virus (HIV) and other lethal viruses. Infections that occur in normal people are generally brief and rarely leave permanent damage. Treatment of this disease requires an aggressive and innovative approach to the development of new treatments so that it requires the role of immunomodulators to improve the immune system. A substance that acts as an enhancer or immune enhancer can be obtained by using herbs that are efficacious as immunostimulants. One of the herbs used is herbal binara (Artemisia vulgaris L) which has been studied as a potential immunomodulator with high antioxidant activity. Previous research also stated that red shoots (Syzygium oleana) were studied as potential immunomodulators with high antioxidant activity. Several other species such as Syzygium samarangense have 16 flavonoida compounds which show pharmacological immunological activity. The purpose of this study was to determine the content of secondary metabolites of ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) and to determine the best dose of extract from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) can reduce the volume of swelling of mouse feet. Examination of the chemical content of secondary metabolites from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) is carried out by chemical screening and characterization of simplicia and extract. The method used is the slow type hypersensitivity method. In this test the independent variable is the secondary metabolite of ethanol extract of herb binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) with four concentrations (50, 100, 200 and 400 mg / kgBB). The positive control used by Stimuno dose is 32.5 mg / kgBB

Investigation of Compounds and Biological Activity of Selected Indonesian Marine Sponges

2020 ◽  
Vol 10 (3) ◽  
pp. 312-321
Author(s):  
Idin Sahidin ◽  
Carla W. Sabandar ◽  
Wahyuni ◽  
Rini Hamsidi ◽  
Sandra Aulia Mardikasari ◽  
...  
Keyword(s):  
Biological Activity ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Marine Sponges ◽  
Chemical Screening ◽  
Methanol Extracts ◽  
Ms Analysis ◽  
Antioxidant Agents

Background: Marine sponges provided a great source of natural products with promising biological activity. This study was aimed to investigate the chemical constituents of methanol extracts of selected Indonesian marine sponges (Callyspongia sp., Clathria sp., Melophlus sarasinorum, and Xestospongia sp.), collected from the Saponda Islands, Southeast Sulawesi, Indonesia as well as to evaluate their antimicrobial and antioxidant activities. Methods: LCMS/MS analysis used to identify the compounds. Agar well diffusion and DPPH assays were used to evaluate the antimicrobial and antioxidant activities. Results: Chemical screening reported alkaloids, terpenoids, steroids, and saponins from all investigated sponges. The LC-MS/MS analysis identified various compounds which mainly contained steroids. Antimicrobial activity (against Bacillus subtilis, Escherichia coli, Salmonella enterica, and Candida albicans) was only shown by the Xestospongia sp. extract. Meanwhile, extracts of M. sarasinorum, Xestospongia sp., and Callyspongia sp. exhibited potent radical scavenging activity. Conclusion: The study concluded that the selected sponges could provide various groups of compounds. Methanol extracts of these sponges could be used as sources of antimicrobial and antioxidant agents.

Vesicular Monoamine Transport Inhibitors

Hypertension ◽  
1996 ◽  
Vol 28 (3) ◽  
pp. 414-420 ◽  
Author(s):  
Manjula Mahata ◽  
Sushil K. Mahata ◽  
Robert J. Parmer ◽  
Daniel T. O'Connor
Keyword(s):  
Monoamine Transport ◽  
Transport Inhibitors
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