scholarly journals Proteomic Analysis of Embryonic Proteins Synthesized from Long-Lived mRNAs During Germination of Rice Seeds

2012 ◽  
Vol 53 (4) ◽  
pp. 687-698 ◽  
Author(s):  
Naoto Sano ◽  
Hadian Permana ◽  
Ryota Kumada ◽  
Yoshihito Shinozaki ◽  
Takanari Tanabata ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Rice Seeds

scholarly journals Proteomic Analysis Reveals Different Involvement of Embryo and Endosperm Proteins during Aging of Yliangyou 2 Hybrid Rice Seeds

2016 ◽  
Vol 7 ◽  
Author(s):  
Ying-Xue Zhang ◽  
Heng-Heng Xu ◽  
Shu-Jun Liu ◽  
Ni Li ◽  
Wei-Qing Wang ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Hybrid Rice ◽  
Endosperm Proteins ◽  
Rice Seeds

Proteomic analysis in different development stages on SP0 generation of rice seeds after space flight

2020 ◽  
Vol 26 ◽  
pp. 34-45 ◽  
Author(s):  
Deyong Zeng ◽  
Jie Cui ◽  
Yishu Yin ◽  
Meng Zhang ◽  
Shan Shan ◽  
...  
Keyword(s):  
Space Flight ◽  
Proteomic Analysis ◽  
Development Stages ◽  
Rice Seeds

scholarly journals Proteomic analysis of stress-related proteins in rice seeds during the desiccation phase of grain filling

2013 ◽  
Vol 30 (2) ◽  
pp. 147-156 ◽  
Author(s):  
Naoto Sano ◽  
Shunpei Masaki ◽  
Takanari Tanabata ◽  
Tetsuya Yamada ◽  
Tadashi Hirasawa ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Grain Filling ◽  
Rice Seeds ◽  
Related Proteins

scholarly journals Shotgun Proteomic Analysis Highlights the Roles of Long-Lived mRNAs and De Novo Transcribed mRNAs in Rice Seeds upon Imbibition

2019 ◽  
Vol 60 (11) ◽  
pp. 2584-2596 ◽  
Author(s):  
Naoto Sano ◽  
Yumiko Takebayashi ◽  
Alexandra To ◽  
Corinne Mhiri ◽  
Lo�c Rajjou ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Energy Production ◽  
De Novo ◽  
Proteome Analysis ◽  
Tca Cycle ◽  
Initial Energy ◽  
Translational Machinery ◽  
Liquid Chromatography Tandem Mass ◽  
Rice Seeds ◽  
Seed Germination Proteins

Abstract During seed germination, proteins are translated not only from mRNAs newly transcribed upon imbibition but also from long-lived mRNAs that are synthesized during seed maturation and stored in the mature dry seeds. To clarify the distinct roles of proteins translated from long-lived mRNAs and de novo transcribed mRNAs in germinating rice embryos, proteome analysis based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combining the use of a transcriptional inhibitor was performed. We observed that α-amanitin significantly represses transcription in germinating embryos; nevertheless, the embryos could germinate, albeit slowly. The proteomic analysis revealed that a total of 109 proteins were translated from long-lived mRNAs associated with germination as well as 222 proteins whose expression were dependent on de novo transcription upon imbibition. Transcriptomic datasets available in public databases demonstrated that mRNAs of the 222 proteins notably increased during germination while those of the 109 proteins highly accumulated in dry embryos and constitutively expressed upon imbibition. Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that many of the 109 proteins from long-lived mRNAs are implicated in energy production such as glycolysis or annotated as nucleotide binding proteins, while the 222 proteins are involved in pathways such as pyruvate metabolism and TCA cycle following glycolysis, and momilactones biosynthesis. We propose that long-lived mRNAs support initial energy production and activation of translational machinery upon imbibition whereas de novo transcription accelerates the energy production after glycolysis, which enables rice seeds to germinate vigorously.

895: Proteomic Analysis of Sera from Bladder Cancer Patients with MALDI-TOF-MS

2007 ◽  
Vol 177 (4S) ◽  
pp. 297-297
Author(s):  
Kristina Schwamborn ◽  
Rene Krieg ◽  
Ruth Knüchel-Clarke ◽  
Joachim Grosse ◽  
Gerhard Jakse
Keyword(s):  
Bladder Cancer ◽  
Cancer Patients ◽  
Proteomic Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Proteomic analysis of the anti-obesity effect of Taeumjowui-Tang in high fat diet-induced obese mice

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
E Changkyun Park ◽  
SY Lee ◽  
SH Yun ◽  
WY Kim ◽  
Y Yi ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
High Fat Diet ◽  
Obese Mice ◽  
High Fat
Sign in / Sign up

Export Citation Format

Share Document