scholarly journals Expression and RNA Interference-Induced Silencing of the Dammarenediol Synthase Gene in Panax ginseng

2006 ◽  
Vol 47 (12) ◽  
pp. 1653-1662 ◽  
Author(s):  
Jung Yeon Han ◽  
Yong Soo Kwon ◽  
Deok Chun Yang ◽  
Young Rim Jung ◽  
Yong Eui Choi
Keyword(s):  
Rna Interference ◽  
Panax Ginseng ◽  
Dammarenediol Synthase

scholarly journals PgMYB2, a MeJA-Responsive Transcription Factor, Positively Regulates the Dammarenediol Synthase Gene Expression in Panax Ginseng

2019 ◽  
Vol 20 (9) ◽  
pp. 2219 ◽  
Author(s):  
Tuo Liu ◽  
Tiao Luo ◽  
Xiangqian Guo ◽  
Xian Zou ◽  
Donghua Zhou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Panax Ginseng ◽  
Growth Regulation ◽  
Ginseng Root ◽  
Myb Transcription Factor ◽  
Mobility Shift ◽  
Binding Interaction ◽  
Mobility Shift Assay ◽  
Dammarenediol Synthase

The MYB transcription factor family members have been reported to play different roles in plant growth regulation, defense response, and secondary metabolism. However, MYB gene expression has not been reported in Panax ginseng. In this study, we isolated a gene from ginseng adventitious root, PgMYB2, which encodes an R2R3-MYB protein. Subcellular localization revealed that PgMYB2 protein was exclusively detected in the nucleus of Allium cepa epidermis. The highest expression level of PgMYB2 was found in ginseng root and it was significantly induced by plant hormones methyl jasmonate (MeJA). Furthermore, the binding interaction between PgMYB2 protein and the promoter of dammarenediol synthase (DDS) was found in the yeast strain Y1H Gold. Moreover, the electrophoretic mobility shift assay (EMSA) identified the binding site of the interaction and the results of transiently overexpressing PgMYB2 in plants also illustrated that it may positively regulate the expression of PgDDS. Based on the key role of PgDDS gene in ginsenoside synthesis, it is reasonable to believe that this report will be helpful for the future studies on the MYB family in P. ginseng and ultimately improving the ginsenoside production through genetic and metabolic engineering.

SNPs of dammarenediol synthase gene were associated with the accumulation of ginsenosides in DAMAYA ginseng , a cultivar of Panax ginseng C. A. Mey.

2016 ◽  
Vol 17 ◽  
pp. 194-200 ◽  
Author(s):  
Chunsong Cheng ◽  
Wenru Wu ◽  
Baoming Huang ◽  
Liang Liu ◽  
Pei Luo ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Dammarenediol Synthase

scholarly journals Alteration of Panax ginseng saponin composition by overexpression and RNA interference of the protopanaxadiol 6-hydroxylase gene (CYP716A53v2)

2016 ◽  
Vol 40 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Seong-Bum Park ◽  
Ju-Hyeon Chun ◽  
Yong-Wook Ban ◽  
Jung Yeon Han ◽  
Yong Eui Choi
Keyword(s):  
Rna Interference ◽  
Panax Ginseng ◽  
Ginseng Saponin ◽  
Hydroxylase Gene

RNA Interference Technology

2005 ◽  
Author(s):  
Andrew Fire ◽  
Marshall Nirenberg
Keyword(s):  
Rna Interference

The potential analgesic and anti-inflammatory activities of Panax ginseng head BuOH fraction in vivo

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
JH Lee ◽  
JH Lee ◽  
YM Lee ◽  
PN Kim ◽  
CS Jeong
Keyword(s):  
Panax Ginseng ◽  
Anti Inflammatory

The oncolytic adenovirus Ad-iREP-2 utilizes p53-dependent rna interference for tumor-selective replication

2008 ◽  
Vol 46 (09) ◽  
Author(s):  
E Gürlevik ◽  
P Schache ◽  
L Zender ◽  
MP Manns ◽  
S Kubicka ◽  
...  
Keyword(s):  
Rna Interference ◽  
Oncolytic Adenovirus ◽  
Tumor Selective

Comparison of quality and ginsenoside contents in roots of Panax ginseng at various ages in Gangwon, Korea

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
HN Jeong ◽  
KJ Lee ◽  
JS Jeong ◽  
YM Mo ◽  
SJ Lee ◽  
...  
Keyword(s):  
Panax Ginseng

Comparison of growth characteristics and quality of Korean ginseng (Panax ginseng C.A. Meyer) using different shade materials

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
HH Kim ◽  
BJ Seong ◽  
KS Lee ◽  
SI Kim ◽  
JM Geun ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Growth Characteristics ◽  
Korean Ginseng
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