scholarly journals Isolation and Characterization of a Novel Peroxidase Gene ZPO-C Whose Expression and Function are Closely Associated with Lignification during Tracheary Element Differentiation

2006 ◽  
Vol 47 (4) ◽  
pp. 493-503 ◽  
Author(s):  
Yasushi Sato ◽  
Taku Demura ◽  
Ken Yamawaki ◽  
Yukina Inoue ◽  
Seiichi Sato ◽  
...  
Keyword(s):  
Tracheary Element ◽  
Tracheary Element Differentiation ◽  
Peroxidase Gene ◽  
Isolation And Characterization ◽  
And Function

scholarly journals Conservation of dishevelled structure and function between flies and mice: isolation and characterization of Dvl2

1996 ◽  
Vol 58 (1-2) ◽  
pp. 15-26 ◽  
Author(s):  
J. Klingensmith ◽  
Y. Yang ◽  
J.D. Axelrod ◽  
D.R. Beier ◽  
N. Perrimon ◽  
...  
Keyword(s):  
Structure And Function ◽  
Isolation And Characterization ◽  
And Function

scholarly journals ISOLATION AND CHARACTERIZATION OF AMBER SUPPRESSORS IN YEAST

Genetics ◽  
1976 ◽  
Vol 82 (2) ◽  
pp. 251-272
Author(s):  
Susan W Liebman ◽  
Fred Sherman ◽  
John W Stewart
Keyword(s):  
Amino Acids ◽  
Cytochrome C ◽  
Yeast Strain ◽  
Normal Amount ◽  
Amber Mutation ◽  
Genetic Analyses ◽  
Isolation And Characterization ◽  
And Function ◽  
Nonsense Suppressors

ABSTRACT Nonsense suppressors were obtained in a haploid yeast strain containing eight nutritional mutations, that are assumed to be amber or ochre, and the cyc1-179 amber mutation that has a UAG codon corresponding to position 9 in iso-1-cytochrome c. Previous studies established that the biosynthesis and function of iso-1-cytochrome c is compatible with replacements at position 9 of amino acids having widely different structures (Stewart and Sherman 1972). UV-induced revertants, selected on media requiring the reversion of one or two of the amber nutritional markers, were presumed to contain a suppressor if there was the unselected reversion of at least one other marker. The 1088 suppressors that were isolated could be divided into 78 phenotypic classes. Only 43 suppressors of three classes caused the production of more than 50% of the normal amount of iso-1-cytochrome c in the cyc1-179 strain. Genetic analyses indicated that all of these highly efficient amber suppressors are allelic to one or another of the eight suppressors which cause the insertion of tyrosine at ochre (UAA) codons (Gilmore, Stewart and Sherman 1971). Furthermore, only tyrosine has been identified at position 9 in iso-1-cytochrome cin cyc1-179 strains suppressed with these efficient amber suppressors.

scholarly journals Isolation and characterization of lung connective-tissue glycoproteins

1982 ◽  
Vol 203 (3) ◽  
pp. 593-601 ◽  
Author(s):  
C Lafuma ◽  
M Moczar ◽  
L Robert
Keyword(s):  
Proteinase Inhibitors ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Gel Filtration ◽  
Lung Parenchyma ◽  
Isolation And Characterization ◽  
Isoelectric Points ◽  
And Function

1. Glycoproteins of hamster, rat and baboon lung parenchyma were investigated by using [14C]glucosamine incorporation in vitro followed by sequential extraction of the macromolecular components and characterization of the glycoproteins in the extracts. 2. Slices of lung parenchyma maintained in vitro incorporated [U-14C]glucosamine linearly with time into non-diffusible macromolecules for up to 5h. All the macromolecule-associated 14C label was present as [14C]glucosamine. 3. These 14C-labelled macromolecules were extracted from previously delipidated and salt-extracted lung by 5M-guanidinium chloride in the presence of dithiothreitol and proteinase inhibitors before (extract A1) and after (extract A2) hydrolysis of the collagen by collagenase. The [14C]glucosamine-labelled glycoproteins in extracts A1 and A2 contained 55 and 5% respectively of the total [14C]glucosamine incorporated in the lung of all three species studied. 4. The [14C]glucosamine-labelled glycoproteins were analysed by gel-filtration chromatography, sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and isoelectric focusing. The major [14C]glucosamine-labelled glycoproteins of baboon lung parenchyma had apparent mol.wts. of about 400 000, 140 000 and 65 000 with isoelectric points respectively of 4.8, 5.4 and 5.4. The hamster lung glycoproteins with isoelectric points of 4.1 and 5.8 were devoid of hydroxyproline and contained galactose, mannose and N-acetylglucosamine. These experiments indicate that several distinct glycoproteins are synthesized in situ by the cells of pulmonary parenchyma and may well play a role in its structure and function.

Dendritic cell precursor populations of mouse blood: identification of the murine homologues of human blood plasmacytoid pre-DC2 and CD11c+ DC1 precursors

Blood ◽  
2003 ◽  
Vol 101 (4) ◽  
pp. 1453-1459 ◽  
Author(s):  
Meredith O'Keeffe ◽  
Hubertus Hochrein ◽  
David Vremec ◽  
Bernadette Scott ◽  
Paul Hertzog ◽  
...  
Keyword(s):  
Human Blood ◽  
Ex Vivo ◽  
Mouse Blood ◽  
Surface Phenotype ◽  
Isolation And Characterization ◽  
Tnf Α ◽  
Close Relationship ◽  
Factor Α ◽  
And Function

Immature and predendritic cells (pre-DCs) of human blood are the most readily accessible human DC sources available for study ex vivo. Murine homologues of human blood DCs have not been described. We report the isolation and characterization of 2 populations of precursor DCs in mouse blood. Mouse blood cells with the surface phenotype CD11cloCD11b−CD45RAhi closely resemble human plasmacytoid cells (or pre-DC2) by morphology and function. On stimulation with oligonucleotides containing CpG motifs (CpG), these cells make large amounts of type 1 interferons and rapidly develop into DCs that bear CD8, though they may be distinct from the CD8+ DCs in the unstimulated mouse. A second population of cells with the surface phenotype CD11c+CD11b+CD45RA− closely resembles the immediate precursors of pre-DC1, rapidly transforming into CD8− DCs after tumor necrosis factor-α (TNF-α) stimulation. These findings indicate the close relationship between human and mouse DCs, provided cells are obtained directly from equivalent source materials.

scholarly journals Isolation and characterization of Ulva prolifera actin1 gene and function verification of the 5′ flanking region as a strong promoter

Bioengineered ◽  
2017 ◽  
Vol 9 (1) ◽  
pp. 124-133 ◽  
Author(s):  
Chunhui Wu ◽  
Peng Jiang ◽  
Yang Guo ◽  
Jianguo Liu ◽  
Jin Zhao ◽  
...  
Keyword(s):  
Ulva Prolifera ◽  
Strong Promoter ◽  
Isolation And Characterization ◽  
Flanking Region ◽  
And Function

scholarly journals Isolation and Characterization of Nanobodies against a Zinc-Transporting P-Type ATPase

Antibodies ◽  
2018 ◽  
Vol 7 (4) ◽  
pp. 39 ◽  
Author(s):  
Elena Longhin ◽  
Christina Grønberg ◽  
Qiaoxia Hu ◽  
Annette Duelli ◽  
Kasper Andersen ◽  
...  
Keyword(s):  
Molecular Level ◽  
Structure And Function ◽  
Structural Studies ◽  
Catalytic Function ◽  
Isolation And Characterization ◽  
Key Players ◽  
Lipid Transporters ◽  
And Function ◽  
P Type

P-type ATPases form a large and ubiquitous superfamily of ion and lipid transporters that use ATP (adenosine triphosphate) to carry out their function. The IB subclass (PIB-ATPases) allows flux of heavy metals and are key players in metal detoxification, critical for human health, crops, and survival of pathogens. Nevertheless, PIB-ATPases remain poorly understood at a molecular level. In this study, nanobodies (Nbs) are selected against the zinc-transporting PIB-ATPase ZntA from Shigella sonnei (SsZntA), aiming at developing tools to assist the characterization of the structure and function of this class of transporters. We identify six different Nbs that bind detergent stabilized SsZntA. We further assess the effect of the Nbs on the catalytic function of SsZntA, and find that five nanobodies associate without affecting the function, while one nanobody significantly reduces the ATPase activity. This study paves the way for more refined mechanistical and structural studies of zinc-transporting PIB-ATPases.

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