scholarly journals An Alloplasmic Male-sterile Line of Brassica oleracea Harboring the Mitochondria from Diplotaxis muralis Expresses a Novel Chimeric Open Reading Frame, orf72

2006 ◽  
Vol 47 (4) ◽  
pp. 549-553 ◽  
Author(s):  
Tomotaka Shinada ◽  
Yosuke Kikuchi ◽  
Ryo Fujimoto ◽  
Sachie Kishitani
Keyword(s):  
Brassica Oleracea ◽  
Open Reading Frame ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Reading Frame

scholarly journals Engineering the AEG1 promoter from cotton to develop male sterile lines

2021 ◽  
Author(s):  
Kamlesh Kumar Soni ◽  
Amita Kush Mehrotra ◽  
Pradeep Kumar Burma
Keyword(s):  
Seed Production ◽  
Reporter Gene ◽  
Tissue Specificity ◽  
Specific Activity ◽  
Open Reading Frame ◽  
Gene Analysis ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Reading Frame ◽  
Regulatory Module

This work reports on modifying the Upstream Regulatory Module (URM, 1.5 Kb region upstream of the open reading frame) of Anther Expressing Gene 1 (AEG1) from cotton to achieve anther specific activity. AEG1 was identified in a previous study aimed to isolate a promoter with tapetum specific activity. Such a promoter could then be used to express barnase and barstar genes for developing male sterile and restorer lines for hybrid seed production in cotton. The AEG1 URM was observed to be active in tapetum as well as in roots making it unusable to drive the expression of barnase gene. Analysis of the URM showed the presence of several root specific motifs. Two modified AEG1 URMs were developed, by removing or mutating these motifs and its activity checked in tobacco. The activity of one of the modified URMs, AEG1(DelBmut) was restricted to the anther tissue as observed using the reporter gene beta-glucuronidase. The study also demonstrates that male sterile lines could be developed in tobacco using the AEG1(DelBmut) URM to express the barnase gene. This work thus shows the possibility of engineering promoters to achieve tissue specificity and to develop male sterile lines in cotton.

scholarly journals Transcript processing internal to a mitochondrial open reading frame is correlated with fertility restoration in male-sterile sorghum

1996 ◽  
Vol 10 (1) ◽  
pp. 123-133 ◽  
Author(s):  
Hoang V. Tang ◽  
Daryl R. Pring ◽  
Lynn C. Shaw ◽  
Reggie A. Salazar ◽  
Figuhr R. Muza ◽  
...  
Keyword(s):  
Fertility Restoration ◽  
Open Reading Frame ◽  
Male Sterile ◽  
Reading Frame ◽  
Transcript Processing

Influence of nuclear background on transcription of a chimeric gene (orf256) and coxI in fertile and cytoplasmic male sterile wheats

Genome ◽  
1994 ◽  
Vol 37 (2) ◽  
pp. 203-209 ◽  
Author(s):  
Jiasheng Song ◽  
Charles Hedgcoth
Keyword(s):  
Mitochondrial Dna ◽  
Open Reading Frame ◽  
Mitochondrial Rna ◽  
Coding Region ◽  
Male Sterile ◽  
Reading Frame ◽  
Cytoplasmic Male Sterile ◽  
Triticum Timopheevi ◽  
Nuclear Background ◽  
Flanking Region

Crosses between Triticum timopheevi, as maternal donor, and T. aestivum can lead to cytoplasmic male sterile (cms) plants. The T. timopheevi derived mitochondrial DNA from parental, cms, and fertility-restored lines differs from that of T. aestivum derived mtDNA in the coxI gene region. Our previous results for cms lines showed that there is an open reading frame, orf256, upstream from coxI in T. timopheevi derived mtDNA that is not present in T. aestivum DNA. The 5′ flanking region and the first 33 nucleotides of the coding region of orf256 are identical to the corresponding region of T. aestivum coxI, whereas the rest of orf256, including the 3′ flank, is not related to coxI. Also, the organization of orf256 and coxI on a HindIII fragment from T. timopheevi derived mtDNA are identical in T. timopheevi, cms, and fertility-restored lines. We now report that the DNA sequence of orf256 is identical in T. timopheevi, cms, and fertility-restored lines. Major transcripts in cms and fertility-restored lines encode both orf256 and coxI with 5′ termini like coxI mRNA of T. aestivum, whereas parental mitochondria from T. timopheevi have major transcripts with 5′ termini within the orf256 coding region. Mitochondria from cms and fertility-restored lines have the potential to produce a protein that would not be present in parental T. timopheevi or in T. aestivum.Key words: cytoplasmic male sterility, wheat, mitochondrial DNA, mitochondrial RNA, coxI.

Expression Characteristic on TaPDC-E1a Gene and Its Regulatory Enzymes Gene in Male Sterile Line of Wheat (Triticum aestivum)

2011 ◽  
Vol 37 (4) ◽  
pp. 620-628
Author(s):  
Long-Yu ZHANG ◽  
Lei YUAN ◽  
Shu-Ling YANG ◽  
Gai-Sheng ZHANG ◽  
Jun-Sheng WANG ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Regulatory Enzymes ◽  
Expression Characteristic ◽  
I Gene

Differential Expression of ATP Synthesis Related Gene in Fertility Conversion of Wheat BNS Male Sterile Line

2014 ◽  
Vol 40 (8) ◽  
pp. 1501
Author(s):  
Zhen WANG ◽  
Xiao-Jing FAN ◽  
Miao ZHANG ◽  
Fang-Ning ZHANG ◽  
Gui-Dong LI ◽  
...  
Keyword(s):  
Differential Expression ◽  
Atp Synthesis ◽  
Related Gene ◽  
Male Sterile ◽  
Male Sterile Line
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