scholarly journals Salicylic Acid Carboxyl Methyltransferase Induced in Hairy Root Cultures of Atropa belladonna after Treatment with Exogeneously Added Salicylic Acid

2002 ◽  
Vol 43 (9) ◽  
pp. 1054-1058 ◽  
Author(s):  
Hiroyuki Fukami ◽  
Tomiko Asakura ◽  
Hiroshi Hirano ◽  
Keiko Abe ◽  
Koichiro Shimomura ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Atropa Belladonna ◽  
Carboxyl Methyltransferase

scholarly journals Enhanced production of valtrate in hairy root cultures of Valeriana jatamansi Jones by methyl jasmonate, jasmonic acid and salicylic acid elicitors

2020 ◽  
Vol 48 (2) ◽  
pp. 839-848
Author(s):  
Shuang ZHAO ◽  
Hong TANG
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Methyl Jasmonate ◽  
Hairy Root ◽  
Hairy Roots ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Potent Inducer ◽  
Valeriana Jatamansi ◽  
Pharmacologically Active

Valtrate is a pharmacologically active epoxyiridoid ester found in the roots and rhizomes of Valeriana jatamansi Jones. The plant produces only small amounts of this metabolite naturally, and so induction of hairy roots as well as elicitation can be useful to increase its commercial production. In this study, strain R1601 of Agrobacterium rhizogenes was used to induce hairy roots in V. jatamansi, and stable hairy root cultures of V. jatamansi were established successfully. The influence of three exogenous elicitors including methyl jasmonate (MJ), jasmonic acid (JA) and salicylic acid (SA) on valtrate production in the hairy root cultures of V. jatamansi was also investigated, and the 25-day-old hairy root cultures were treated with different concentrations of the elicitors at exposure time of 7 days. This present study showed that MJ (100 mg/L) highly promoted valtrate production at 7 days after elicitation, to a level of 3.63 times higher than that of non-elicited control. SA did not significantly increase the production of valtrate. This is the first-time study to assess the elicitation of hairy root cultures to promote valtrate biosynthesis in V. jatamansi and the resulting experiments demonstrated that MJ was indeed a potent inducer of valtrate biosynthesis.

Induction of Pyridine Alkaloid Formation in Transformed Root Cultures of Nicotiana tabacum

2009 ◽  
Vol 64 (11-12) ◽  
pp. 869-874 ◽  
Author(s):  
Rawia Zayed ◽  
Michael Wink
Keyword(s):  
Salicylic Acid ◽  
Nicotiana Tabacum ◽  
Hairy Root ◽  
Inhibitory Effect ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Fresh Weight ◽  
Root Tips ◽  
Transformed Root Cultures ◽  
Set Up

Hairy root cultures of Nicotiana tabacum were set up by excised root tips with Agrobacterium rhizogenes. The successful transformation was confirmed by analyzing rolC and virC genes using polymerase chain reaction (PCR). Hairy root cultures were employed to study the formation of pyridine alkaloids, mainly nicotine. The transformed cultures were incubated with potential elicitors, such as methyljasmonate, quercetin and salicylic acid, in order to stimulate the biosynthesis of pyridine alkaloids. Profile and amounts of pyridine alkaloids were analyzed using capillary GLC-MS. Treatment of the cultures with methyljasmonate (50 μM) increased the alkaloid accumulation ca. 7-fold up to a level of 0.58 mg/g fresh weight as compared to untreated controls. Quercetin (200 μM) enhanced the alkaloid production ca. 4-fold (0.34 mg/g fresh weight) within 24 h. In contrast salicylic acid in all tested concentrations decreased the alkaloid level to 1 μg/g fresh weight. Also the inhibitory effect of salicylic acid on the elicitation effect of methyljasmonate and quercetin was investigated.

scholarly journals Daidzein Production and HeLa Cytotoxicity of Bituminaria bituminosa Hairy Root Cultures

2017 ◽  
Vol 12 (11) ◽  
pp. 1934578X1701201
Author(s):  
Francesca D'Angiolillo ◽  
Cecilia Noccioli ◽  
Barbara Ruffoni ◽  
Roberto Scarpato ◽  
Luisa Pistelli ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Hairy Root ◽  
Folk Medicine ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Polyphenol Content ◽  
Antiproliferative Effects ◽  
Root Extracts ◽  
Bituminaria Bituminosa

Bituminaria bituminosa (L.) C.H. Stirt is a perennial species widely distributed in the Mediterranean basin and the Canary Islands. This species is used in folk medicine and currently has considerable pharmaceutical interest for its content in phenylpropanoids, furanocoumarins and pterocarpans. In vitro cultures (shoots and hairy roots) have been performed to obtain plant material useful for the production of these metabolites. Hairy root cultures were successfully established after inoculation of hypocotyls with the LBA 9402 A. rhizogenes strain. The HRPB3 line was selected for further analysis and elicited with chitosan and salicylic acid. All the HRPB3 cultures showed higher polyphenol content and greater DPPH-antioxidant activity than shoots cultured in vitro. The presence of isoflavone daidzein was detected in the hairy root extracts. The cytotoxic effect of HR extracts has been further tested on HeLa cells: the salicylic acid elicited HR exhibited good antiproliferative effects.

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