scholarly journals pilG Gene Cluster and Split pilL Genes Involved in Pilus Biogenesis, Motility and Genetic Transformation in the Cyanobacterium Synechocystis sp. PCC 6803

2002 ◽  
Vol 43 (5) ◽  
pp. 513-521 ◽  
Author(s):  
Shizue Yoshihara ◽  
Xiaoxing Geng ◽  
Masahiko Ikeuchi
Keyword(s):  
Genetic Transformation ◽  
Gene Cluster ◽  
Pilus Biogenesis ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Analysis of promoter regions in the saxitoxin gene cluster identifies potential bottlenecks in heterologous biosynthesis

2019 ◽  
Author(s):  
Paul Michael D'Agostino ◽  
Bakir Al-Sinawi ◽  
Rabia Mazmouz ◽  
Julia Muenchhoff ◽  
Brett Anthony Neilan ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Local Anaesthetics ◽  
Bidirectional Promoter ◽  
Biosynthetic Gene Cluster ◽  
Reporter System ◽  
Promoter Regions ◽  
E Coli ◽  
Synechocystis Sp ◽  
Pcc 6803

Abstract Background: Dolichospermum circinale is a filamentous bloom-forming cyanobacterium responsible for biosynthesis of the paralytic shellfish toxins (PST), including saxitoxin. PSTs are neurotoxins and in their purified form are important analytical standards for monitoring the quality of water and seafood and biomedical research tools for studying neuronal sodium channels. More recently, PSTs have been recognised for their utility as local anaesthetics. Characterisation of the transcriptional elements within the saxitoxin ( sxt ) biosynthetic gene cluster (BGC) is a first step towards accessing these molecules for biotechnology. Results: In D. circinale AWQC131C the sxt BGC is transcribed from two bidirectional promoter regions encoding five individual promoters. These promoters were identified experimentally using 5ʹ RACE and their activity assessed via coupling to a lux reporter system in E. coli and Synechocystis sp. PCC 6803. Transcription of the predicted drug/metabolite transporter (DMT) encoded by sxtPER was found to initiate from two promoters, P sxtPER and P sxtPER2 . In E. coli , strong expression of lux from P sxtP , P sxtD and P sxtPER was observed while expression from P orf24 and P sxtPER2 was remarkably weaker. In contrast, heterologous expression in Synechocystis sp. PCC 6803 showed that expression of lux from P sxtP , P sxtPER , and P orf24 promoters was statistically higher compared to the non-promoter control, while P sxtD showed poor activity under the described conditions. Conclusions: Both of the heterologous hosts investigated in this study exhibited high expression levels from three of the five sxt promoters. These results indicate that future experiments to clone the complete sxt BGC into either heterologous host should result in the transcription of the complete pathway following engineering of the least active promoters. Therefore, heterologous expression of the sxt BGC in either E. coli or Synechocystis could be a viable option for producing PSTs for industrial or biomedical purposes.

scholarly journals A Gene Cluster Involved in Metal Homeostasis in the Cyanobacterium Synechocystis sp. Strain PCC 6803

2000 ◽  
Vol 182 (6) ◽  
pp. 1507-1514 ◽  
Author(s):  
Mario García-Domínguez ◽  
Luis Lopez-Maury ◽  
Francisco J. Florencio ◽  
José C. Reyes
Keyword(s):  
Gene Cluster ◽  
Metal Binding ◽  
Open Reading Frames ◽  
Response System ◽  
Metal Binding Domain ◽  
Carboxy Terminal Region ◽  
Carboxy Terminal ◽  
Reading Frames ◽  
Synechocystis Sp ◽  
Pcc 6803

ABSTRACT A gene cluster composed of nine open reading frames (ORFs) involved in Ni2+, Co2+, and Zn2+ sensing and tolerance in the cyanobacterium Synechocystis sp. strain PCC 6803 has been identified. The cluster includes an Ni2+response operon and a Co2+ response system, as well as a Zn2+ response system previously described. Expression of the Ni2+ response operon (nrs) was induced in the presence of Ni2+ and Co2+. Reduced Ni2+ tolerance was observed following disruption of two ORFs of the operon (nrsA and nrsD). We also show that the nrsD gene encodes a putative Ni2+ permease whose carboxy-terminal region is a metal binding domain. The Co2+ response system is composed of two divergently transcribed genes, corR and corT, mutants of which showed decreased Co2+ tolerance. Additionally, corR mutants showed an absence of Co2+-dependent induction of corT, indicating that CorR is a transcriptional activator of corT. To our knowledge, CorR is the first Co2+-sensing transcription factor described. Our data suggest that this region of theSynechocystis sp. strain PCC 6803 genome is involved in sensing and homeostasis of Ni2+, Co2+, and Zn2+.

The role of Slr1443 in pilus biogenesis in Synechocystis sp. PCC 6803: involvement in post-translational modification of pilins

2004 ◽  
Vol 315 (1) ◽  
pp. 179-186 ◽  
Author(s):  
Young Hye Kim ◽  
Young Mok Park ◽  
Soo-Jung Kim ◽  
Youn-Il Park ◽  
Jong-Soon Choi ◽  
...  
Keyword(s):  
Post Translational Modification ◽  
Pilus Biogenesis ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Cloning of the psbK gene from Synechocystis sp. PCC 6803 and characterization of photosystem II in mutants lacking PSII-K

1991 ◽  
Vol 266 (17) ◽  
pp. 11111-11115
Author(s):  
M. Ikeuchi ◽  
B. Eggers ◽  
G.Z. Shen ◽  
A. Webber ◽  
J.J. Yu ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Synechocystis Sp ◽  
Pcc 6803
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