scholarly journals Enhanced Susceptibility of Photosynthesis to Low-Temperature Photoinhibition due to Interruption of Chill-Induced Increase of S-Adenosylmethionine Decarboxylase Activity in Leaves of Spinach (Spinacia oleracea L.)

2002 ◽  
Vol 43 (2) ◽  
pp. 196-206 ◽  
Author(s):  
Lixiong He ◽  
Kazuyoshi Nada ◽  
Yoshihisa Kasukabe ◽  
Shoji Tachibana
Keyword(s):  
Low Temperature ◽  
Spinacia Oleracea ◽  
Decarboxylase Activity ◽  
Adenosylmethionine Decarboxylase ◽  
Spinacia Oleracea L

Long-term effects of temperature shifts on xanthophyll cycle and photoinhibition in spinach (Spinacia oleracea)

1999 ◽  
Vol 26 (2) ◽  
pp. 125 ◽  
Author(s):  
G. H. Krause ◽  
N. Carouge ◽  
H. Garden
Keyword(s):  
Low Temperature ◽  
Xanthophyll Cycle ◽  
Spinacia Oleracea ◽  
Temperature Shift ◽  
Long Term Effects ◽  
Xanthophyll Cycle Pigments ◽  
Spinacia Oleracea L ◽  
Autumn And Winter ◽  
Kinetics Of

The present study tested with spinach (Spinacia oleracea L.), whether leaves are capable of long-term acclimative responses of carotenoids when warm-grown (20°C) plants were subjected to a regime of low temperature (1–6°C) and excess light (250 mol m–2 s–1). About 17 days after the temperature shift, leaves of the third leaf pair were compared with the respective leaves of warm-grown control plants. The cold-treated leaves exhibited reduced susceptibility to photoinhibition (at 4°C) and considerably faster kinetics of ‘recovery’ (at 20°C), as determined by changes in the ratio of dark-adapted variable to maximum chlorophyll fluorescence, FV/FM. The temperature shift induced marked changes in the composition of photosynthetic pigments. In particular, the pool of xanthophyll cycle pigments, viola-, anthera- and zeaxanthin, based on chlorophyll a+b, was enlarged by about 50%. The proportion of xanthophyll cycle pigments referred to the sum of carotenoids increased by about 25% and, in excessive light, a larger fraction of violaxanthin became deepoxidized. Overall, in respect of carotenoid composition and xanthophyll cycle activity, leaves that had been acclimated by temperature shift were very similar to leaves acclimated by growth in the field during autumn and winter. The data show that in spinach leaves, photoprotective mechanisms can be induced by temperature shift without requirement for development and growth at low temperature.

Versuche zur präparativen Isolierung pflanzlicher Zellorganellen mittels der trägerfreien, kontinuierlichen Ablenkungselektrophorese

1964 ◽  
Vol 19 (11) ◽  
pp. 1072-1075 ◽  
Author(s):  
K. Hannig ◽  
W. Klofat ◽  
H. Endres
Keyword(s):  
Spinacia Oleracea ◽  
Taraxacum Officinale ◽  
Wird Eine ◽  
Spinacia Oleracea L ◽  
Helianthus Annus

Es wird eine präparative Methode zur Isolierung pflanzlicher Zellbestandteile mittels der trägerfreien, kontinuierlichen Ablenkungselektrophorese nach HANNIG 1, 2 kurz skizziert. Als Versuchsmaterial dienen Blätter von Spinat (Spinacia oleracea L.), Sonnenblumen (Helianthus annus L.) und Löwenzahn (Taraxacum officinale Web.).Die Verteilungskurven werden durch Extinktionsmessung der einzelnen Fraktionen in den Auffanggläschen erhalten. Die Definition der Teilchen erfolgt vorläufig morphologisch durch Anfärben und lichtmikroskopische Prüfung als auch durch elektronenmikroskopische Kontrolle nach vorheriger Präparation nach dem „negativ staining“ Verfahren 3,4 oder Kontrastierung mit Phosphorwolframsäure.Bei der Trennung werden Zellkerne und Zellkernfragmente, ganze Chloroplasten, „Mitochondrien“ und plasmatische Strukturen erhalten.

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